HLA-DR antigen frequencies in Mexican patients with dengue virus infection: HLA-DR4 as a possible genetic resistance factor for dengue hemorrhagic fever

2002 ◽  
Vol 63 (11) ◽  
pp. 1039-1044 ◽  
Author(s):  
Curtis LaFleur ◽  
Julio Granados ◽  
Gilberto Vargas-Alarcon ◽  
Jorge Ruíz-Morales ◽  
Cynthia Villarreal-Garza ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Genetic Resistance ◽  
Hemorrhagic Fever ◽  
Resistance Factor ◽  
Dengue Virus Infection ◽  
Hla Dr ◽  
Mexican Patients

scholarly journals The Role Activity of Complement, TNFα & IL12 in Pathogenesis Dengue Virus

2010 ◽  
Vol 1 (1) ◽  
pp. 44
Author(s):  
Soegeng Soegijanto ◽  
Dian Dwi Sary ◽  
Budi Setiawan ◽  
Atsushi Yamanaka
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Study Groups ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Complement Activity ◽  
Grade Iii

Dengue Virus infection is always found in some part of the world especially South East Asia including Indonesia. The pathogenesis of Dengue Virus infection is still controversial. The aim of this study is to analyze the role complement activity, TNFα & IL12 in Dengue Virus infection especially in pathogenesis of Dengue Virus infection. Cross sectional study had been done since February 2009 in Dr. Soetomo Hospital Surabaya. Blood Sera of Dengue Virus infection were collected from Dengue Fever, and Dengue Hemorrhagic Fever patient who had been care in Paediatric. Dengue patients and time schedule for taking blood sample for examination CH50, TNFα & IL12 as follow: on the first day on admission, the second day, the third day. Study groups of patients as follow: Dengue Fever, 36; Dengue Hemorrhagic Fever grade I, 37; Dengue Hemorrhagic Fever grade II, 10; Dengue Hemorrhagic Fever grade III, 18; Dengue Hemorrhagic Fever grade IV, 6. In this study found that the higher activity complement which lower level CH50 was more identified on Dengue Shock Syndrome and Dengue Hemorrhagic Fever grade III than Dengue Fever cases. A concept of our study was focusing on manifestation of vascular leakage, measurement of complement activity CH50, TNFα & IL12 and clinical manifestation Dengue Hemorrhagic Fever. The examination of TNFα & IL12 in our study supported the role the activity complement. The conclusion are measurement CH50, TNFα & IL12 can be used as a predictive factor of the degree of Dengue Virus infection

scholarly journals LIMFOSIT PLASMA BIRU DAN JUMLAH LEUKOSIT PADA PASIEN ANAK INFEKSI VIRUS DENGUE DI MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Rosnatalia D. Arruan ◽  
Glady Rambert ◽  
Firginia Manoppo
Keyword(s):  
Informed Consent ◽  
Virus Infection ◽  
Dengue Virus ◽  
Laboratory Test ◽  
Dengue Hemorrhagic Fever ◽  
Blood Test ◽  
Hemorrhagic Fever ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Routine Blood

Abstract: Dengue is an arboviral disease that is widely spread across the world by Aedes mosquitoes. The early onset of dengue virus infection is difficult to identify due to indistinctive clinical symptoms. Therefore, simple laboratory test is required in the early diagnosis of dengue virus infection. Laboratory test that include routine blood test and blue plasma lymphocyte are important as rapid and relatively cheap diagnostic tool. Blue plasma lymphocytes derived from lymphoid which acts as an immune response that can be found in 4% of peripheral blood on 98% dengue hemorrhagic fever cases. Besides the increases in blue plasma lymphocytes, dengue virus infection can also causes leucopenia. Leucopenia in Dengue Hemorrhagic Fever (DHF) is characterized by the decrease of neutrophile count that happened during the early critical phase (third day of fever). This is a cross-sectional study using samples of 37 children who are infected with dengue virus in RS Advent Manado, RS TNI AD Wolter Monginsidi, RS Pancaran Kasih Manado during December 2014 to January 2015. Inclusion criteria are children below age 15, who have NS1 or IgG/IgM positive, who are on the third until seventh day of fever, whose parents or guardian have signed the informed consent. Conclusion: Leucopenia (leukocyte count below 4000/mm3) was found in children with dengue virus infection who had their routine blood test. Blue plasma lymphocytes are not always found in patients with NS1-AG, IgG/IgM positive.Keywords: dengue virus infection, leucocyte, blue plasma lymphocyteAbstrak: Dengue adalah penyakit arboviral yang paling banyak tersebar di dunia yang ditularkan nyamuk Aedes. Awal infeksi virus dengue sulit untuk dikenali sebab tidak menunjukkan gejala klinis khas. Karena itu, pemeriksaan laboratorium sederhana dapat berperan dalam pengenalan infeksi virus, adalah pemeriksaan darah rutin dan limfosit plasma biru yang cepat dan murah. Limfosit plasma biru sebagai respon imun dengan persentase 4% di darah tepi pada 98% kasus demam beradarah dengue. Selain peningkatan limfosit plasma biru, infeksi virus dengue juga dapat menyebabkan leukopenia. Leukopenia pada demam berdarah dengue didominasi penurunan jumlah neutrofil yang terjadi pada awal fase kritis (hari ketiga demam). Penelitian ini menggunakan desain cross sectional dengan sampel penelitian 37 pasien anak yang terinfeksi virus dengue di RS Advent Manado, RS TNI AD Wolter Mongisidi, RS Pancaran Kasih Manado pada bulan Desember 2014 sampai Januari 2015. Kriteria masukan adalah Anak usia kurang dari 15 tahun, hari ketiga sampai ketujuh demam, NS1 atau IgG/IgM positif, Orangtua atau wali bersedia menandatangani informed consent. Simpulan: Pada pasien anak dengan infeksi virus dengue yang dilakukan pemeriksaan darah rutin ditemukan adanya leukopenia dengan jumlah leukosit dibawah 4000/mm3.Kata kunci: infeksi virus dengue, leukosit, limfosit plasma biru

scholarly journals Gambaran Klinis dan Respon Imun Penderita Demam Berdarah Dengue di Rumah Sakit Kristen Lindi Mara Sumba Timur Selama Bulan Januari Sampai dengan Desember 2018

2020 ◽  
pp. 209-216
Author(s):  
Ruben Wadu Wila ◽  
Roy Nusa
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Features ◽  
Dengue Hemorrhagic Fever ◽  
Clinical Symptoms ◽  
Secondary Infection ◽  
Hemorrhagic Fever ◽  
The Body ◽  
Dengue Virus Infection ◽  
Infected Female

Dengue hemorrhagic fever (DHF) is an arboviral disease that is widespread worldwide. Dengue virus is transmitted to humans through the bite of an infected female Aedes mosquito. Infection of one of the viral serotypes in the majority of cases causes no symptoms or may cause clinical symptoms. Dengue virus infection causes the body to respond clinically and form antibodies. This research study is to provide clinical features and immune response in patients with Dengue hemorrhagic fever. This article uses an observational study design of secondary data of both IgM and IgG sequential examinations of 52 people with Dengue hemorrhagic fever infections at Lindi maria hospital in 2018.The results of the descriptive analysis showed that male population with Dengue hemorrhagic fever was 55.8%, and the highest rate of infection was in adolescents 34.6%. The most clinical features found were fever 91.4%; headache 24.0%; swallow pain 20.8%; vomiting 21.8%; and gastric pain18.7%. Serological examination showed 19.2% positive IgM; 36.5% IgG; and 44.2% positive both IgM and IgG. The Dengue virus infection in 52 patients in East Sumba is mostly secondary infection (80.8%) with the most infected age group is the adult group (≥ 16 years old).

scholarly journals CORRELATION OF DENGUE VIRUS SEROTYPE AND DVI SEVERITY IN ADULT PATIENTS

2018 ◽  
Vol 24 (2) ◽  
pp. 185
Author(s):  
Suci Andriani ◽  
Aryati Aryati ◽  
Usman Hadi
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Real Time ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Adult Patients ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
Virus Serotype ◽  
Degree Of Severity

The clinical manifestation of dengue virus infection is often not clear, varies widely from mild to severe. Exposure of dengue virus which serotype is different from a previous infection is a risk factor for the severe manifestation of dengue virus infection. Dengue hemorrhagic fever is classified into four degrees of severity based on clinical manifestations and laboratory results. Real-time RT-PCR Dengue can detect dengue virus serotype in early dengue virus infection. The aimed of this study was to prove the correlation between dengue virus serotype and degree of severity in adult patients. This study was a cross-sectional observational design done in February until July 2016. Subjects consisted of 100 dengue virus infection patients. Serum of the patients was examined using Real-time RT-PCR Dengue (Simplexa™ Dengue). It was shown that from 46 patients with DENV-3 serotype was 63%, DENV-2 serotype 17.4%, DENV-1 serotypes 17.4% and mixed infection of DENV-1 and DENV-3 serotype 2.2%. There was not any DENV-4 serotype. Dengue Hemorrhagic Fever (DHF) stage I was 47.8%, DHF stage II was 30.4%, DHF stage III was 10.9% and Dengue Fever was 10.9%. There was not any DHF stage IV. There was not enough evidence that DENV-3 correlated with the degree of severity (p= 0.510). Based on this research, DENV-3 serotype was the dominant serotype prevalent at the Dr. Soetomo Hospital. There was no correlation between viral dengue serotype and severity in dengue adult patients in this study. 

scholarly journals UPDATE MANAGEMENT OF DENGUE COMPLICATION IN PEDIATRIC

2015 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Soegeng Soegijanto
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Performance ◽  
Kidney Injury ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Liver Involvement ◽  
Dengue Virus Infection ◽  
Important Health ◽  
Grade Iii

Dengue virus infection is one of the important health problems in Indonesia, although the mortality rate has been decreased but many dengue shock syndrome cases is very difficult to be solving handled. It might be due to nature course of dengue virus infection is very difficult to predict of the earlier time of severity occur. THE AIM To get idea to make update management of dengue complication in pediatric. MATERIAL AND METHOD Data were compiled from Dr. Soetomo Hospital Surabaya in 2009. The diagnosis of all cases was based on criteria WHO 1997 and PCR examination in Institute Tropical Disease for identified serotype of dengue virus infection. The unusual cases of dengue virus infection were treated following the new WHO protocol in 2009. RESULT There were only 3 cases with serotype DEN 1, consisted 2 cases had age 1–4 years and 1 had age 5–14 years. 2 cases showed a severe clinical performance as dengue shock syndrome and 1 case showed as unusual case of dengue virus infection. Three report cases of: a. Dengue hemorrhagic fever grade III which liver involvement and had bilateral pleural effusion; b. Dengue hemorrhagic grade III with liver involvement and encephalopathy; c. Dengue hemorrhagic grade III with liver involvement acute kidney injury, myocardial involvement and encephalopathy. All the patients were treated according to new edition WHO protocol and all of the involving organ recovered along with the improvement of the disease. CONCLUSION Update management of dengue complication pediatric should be learned carefully used for helping unusual cases of dengue virus infection.

scholarly journals Roles for Endothelial Cells in Dengue Virus Infection

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Nadine A. Dalrymple ◽  
Erich R. Mackow
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Capillary Permeability ◽  
Virus Disease ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Dengue Virus Infection ◽  
Barrier Functions ◽  
Dengue Disease ◽  
Primary Fluid

Dengue viruses cause two severe diseases that alter vascular fluid barrier functions, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The endothelium is the primary fluid barrier of the vasculature and ultimately the effects of dengue virus infection that cause capillary leakage impact endothelial cell (EC) barrier functions. The ability of dengue virus to infect the endothelium provides a direct means for dengue to alter capillary permeability, permit virus replication, and induce responses that recruit immune cells to the endothelium. Recent studies focused on dengue virus infection of primary ECs have demonstrated that ECs are efficiently infected, rapidly produce viral progeny, and elicit immune enhancing cytokine responses that may contribute to pathogenesis. Furthermore, infected ECs have also been implicated in enhancing viremia and immunopathogenesis within murine dengue disease models. Thus dengue-infected ECs have the potential to directly contribute to immune enhancement, capillary permeability, viremia, and immune targeting of the endothelium. These effects implicate responses of the infected endothelium in dengue pathogenesis and rationalize therapeutic targeting of the endothelium and EC responses as a means of reducing the severity of dengue virus disease.

scholarly journals Upaya Pencegahan dan Pengendalian Demam Berdarah Dengue dalam Bentuk Peta Tematik di Kecamatan Rappocini

bionature ◽  
2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Suci Wulandhani ◽  
A. Bida Purnamasari ◽  
Ryan Humardani Syam Pratomo
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Hemorrhagic Fever ◽  
Viral Disease ◽  
High Rate ◽  
Dengue Virus Infection ◽  
Dengue Disease ◽  
Keywords Prevention ◽  
Short Time ◽  
And Control

Abstract. Dengue hemorrhagic fever (DHF) is a disease caused by dengue virus infection. One symptom of dengue virus infection is high fever and headache. Dengue virus is a virus from the genus Flavivirus, family of Flaviviridae. This dengue fever is a dangerous viral disease because it can cause sufferers to die in a very short time / several days. The existence and population density are often associated with transmission, endemicity and Extraordinary Events (EE) of DHF. Disease mapping by utilizing digital technology to support epidemiological investigations and also as a tool to monitor regional conditions for dengue disease. The purpose of this study was to determine efforts to prevent and control dengue disease by mapping. The results of the study show that mapping can be seen as increasing and decreasing the number of cases of dengue disease so that it can assist in data management and reporting of information to monitor areas at risk of contracting dengue disease. Suggestions given need to do further research using other variables that are the cause of the high rate of dengue cases, so that it can be utilized by related agencies as the basis of the information system supporting the decision on preventive measures to combat dengue. Keywords: prevention and control, DHF, mapping.

scholarly journals Induction of interferon alpha from human lymphocytes by autologous, dengue virus-infected monocytes.

1987 ◽  
Vol 166 (4) ◽  
pp. 999-1010 ◽  
Author(s):  
I Kurane ◽  
F A Ennis
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Human Lymphocytes ◽  
Killer Cell ◽  
Cell Contact ◽  
Dengue Virus Infection ◽  
Ifn Gamma ◽  
Ifn Alpha ◽  
Hla Dr ◽  
Cell Fractions

Human monocytes actively replicate dengue virus. To dissect the primary immune responses to dengue virus-infected monocytes (DV-monocytes), we analyzed the interaction between autologous DV-monocytes and the peripheral blood lymphocytes (PBL) of dengue nonimmune donors. Interferon (IFN) activity was detected when PBL were cultured with DV-monocytes. Cell contact between PBL and DV-monocytes was required for IFN production; however, MHC compatibility between PBL and monocytes was not necessary. DV-monocytes fixed with paraformaldehyde or glutaraldehyde, which produced no infectious virus, also induced high levels of IFN from PBL. The ability of DV-monocytes to induce IFN correlated with the appearance of dengue antigens. The PBL that produce IFN were characterized by FACS sorting using monoclonal and polyclonal antibodies. HLA-DR+ and T3- cells produced high titers of IFN, while HLA-DR- and T3+ cells produced very low or undetectable levels of IFN. Moderate titers of IFN were produced by cells contained in B cell fractions (surface immunoglobulin-positive, B1+, and Leu-12+), and cells contained in natural killer cell fractions (Leu-11+ and OKM1+). Therefore, IFN-producing cells are heterogeneous, and the predominant producer cells are characterized as HLA-DR+ and non-T lymphocytes. The IFN produced was characterized by RIA using mAbs to IFN-alpha and IFN-gamma. The IFN-alpha was the predominant IFN produced; in addition, a low level of IFN-gamma was also detected in some experiments. The culture fluids obtained from PBL exposed to autologous DV-monocytes, which contained high IFN activity, completely inhibited dengue virus infection of monocytes. These results suggest that IFN-alpha produced by PBL exposed to DV-monocytes may play an important role in controlling primary dengue virus infection.

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