scholarly journals Gambaran Klinis dan Respon Imun Penderita Demam Berdarah Dengue di Rumah Sakit Kristen Lindi Mara Sumba Timur Selama Bulan Januari Sampai dengan Desember 2018

2020 ◽  
pp. 209-216
Author(s):  
Ruben Wadu Wila ◽  
Roy Nusa
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Features ◽  
Dengue Hemorrhagic Fever ◽  
Clinical Symptoms ◽  
Secondary Infection ◽  
Hemorrhagic Fever ◽  
The Body ◽  
Dengue Virus Infection ◽  
Infected Female

Dengue hemorrhagic fever (DHF) is an arboviral disease that is widespread worldwide. Dengue virus is transmitted to humans through the bite of an infected female Aedes mosquito. Infection of one of the viral serotypes in the majority of cases causes no symptoms or may cause clinical symptoms. Dengue virus infection causes the body to respond clinically and form antibodies. This research study is to provide clinical features and immune response in patients with Dengue hemorrhagic fever. This article uses an observational study design of secondary data of both IgM and IgG sequential examinations of 52 people with Dengue hemorrhagic fever infections at Lindi maria hospital in 2018.The results of the descriptive analysis showed that male population with Dengue hemorrhagic fever was 55.8%, and the highest rate of infection was in adolescents 34.6%. The most clinical features found were fever 91.4%; headache 24.0%; swallow pain 20.8%; vomiting 21.8%; and gastric pain18.7%. Serological examination showed 19.2% positive IgM; 36.5% IgG; and 44.2% positive both IgM and IgG. The Dengue virus infection in 52 patients in East Sumba is mostly secondary infection (80.8%) with the most infected age group is the adult group (≥ 16 years old).

scholarly journals The Role Activity of Complement, TNFα & IL12 in Pathogenesis Dengue Virus

2010 ◽  
Vol 1 (1) ◽  
pp. 44
Author(s):  
Soegeng Soegijanto ◽  
Dian Dwi Sary ◽  
Budi Setiawan ◽  
Atsushi Yamanaka
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Study Groups ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Complement Activity ◽  
Grade Iii

Dengue Virus infection is always found in some part of the world especially South East Asia including Indonesia. The pathogenesis of Dengue Virus infection is still controversial. The aim of this study is to analyze the role complement activity, TNFα & IL12 in Dengue Virus infection especially in pathogenesis of Dengue Virus infection. Cross sectional study had been done since February 2009 in Dr. Soetomo Hospital Surabaya. Blood Sera of Dengue Virus infection were collected from Dengue Fever, and Dengue Hemorrhagic Fever patient who had been care in Paediatric. Dengue patients and time schedule for taking blood sample for examination CH50, TNFα & IL12 as follow: on the first day on admission, the second day, the third day. Study groups of patients as follow: Dengue Fever, 36; Dengue Hemorrhagic Fever grade I, 37; Dengue Hemorrhagic Fever grade II, 10; Dengue Hemorrhagic Fever grade III, 18; Dengue Hemorrhagic Fever grade IV, 6. In this study found that the higher activity complement which lower level CH50 was more identified on Dengue Shock Syndrome and Dengue Hemorrhagic Fever grade III than Dengue Fever cases. A concept of our study was focusing on manifestation of vascular leakage, measurement of complement activity CH50, TNFα & IL12 and clinical manifestation Dengue Hemorrhagic Fever. The examination of TNFα & IL12 in our study supported the role the activity complement. The conclusion are measurement CH50, TNFα & IL12 can be used as a predictive factor of the degree of Dengue Virus infection

HLA-DR antigen frequencies in Mexican patients with dengue virus infection: HLA-DR4 as a possible genetic resistance factor for dengue hemorrhagic fever

2002 ◽  
Vol 63 (11) ◽  
pp. 1039-1044 ◽  
Author(s):  
Curtis LaFleur ◽  
Julio Granados ◽  
Gilberto Vargas-Alarcon ◽  
Jorge Ruíz-Morales ◽  
Cynthia Villarreal-Garza ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Genetic Resistance ◽  
Hemorrhagic Fever ◽  
Resistance Factor ◽  
Dengue Virus Infection ◽  
Hla Dr ◽  
Mexican Patients

scholarly journals LIMFOSIT PLASMA BIRU DAN JUMLAH LEUKOSIT PADA PASIEN ANAK INFEKSI VIRUS DENGUE DI MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Rosnatalia D. Arruan ◽  
Glady Rambert ◽  
Firginia Manoppo
Keyword(s):  
Informed Consent ◽  
Virus Infection ◽  
Dengue Virus ◽  
Laboratory Test ◽  
Dengue Hemorrhagic Fever ◽  
Blood Test ◽  
Hemorrhagic Fever ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Routine Blood

Abstract: Dengue is an arboviral disease that is widely spread across the world by Aedes mosquitoes. The early onset of dengue virus infection is difficult to identify due to indistinctive clinical symptoms. Therefore, simple laboratory test is required in the early diagnosis of dengue virus infection. Laboratory test that include routine blood test and blue plasma lymphocyte are important as rapid and relatively cheap diagnostic tool. Blue plasma lymphocytes derived from lymphoid which acts as an immune response that can be found in 4% of peripheral blood on 98% dengue hemorrhagic fever cases. Besides the increases in blue plasma lymphocytes, dengue virus infection can also causes leucopenia. Leucopenia in Dengue Hemorrhagic Fever (DHF) is characterized by the decrease of neutrophile count that happened during the early critical phase (third day of fever). This is a cross-sectional study using samples of 37 children who are infected with dengue virus in RS Advent Manado, RS TNI AD Wolter Monginsidi, RS Pancaran Kasih Manado during December 2014 to January 2015. Inclusion criteria are children below age 15, who have NS1 or IgG/IgM positive, who are on the third until seventh day of fever, whose parents or guardian have signed the informed consent. Conclusion: Leucopenia (leukocyte count below 4000/mm3) was found in children with dengue virus infection who had their routine blood test. Blue plasma lymphocytes are not always found in patients with NS1-AG, IgG/IgM positive.Keywords: dengue virus infection, leucocyte, blue plasma lymphocyteAbstrak: Dengue adalah penyakit arboviral yang paling banyak tersebar di dunia yang ditularkan nyamuk Aedes. Awal infeksi virus dengue sulit untuk dikenali sebab tidak menunjukkan gejala klinis khas. Karena itu, pemeriksaan laboratorium sederhana dapat berperan dalam pengenalan infeksi virus, adalah pemeriksaan darah rutin dan limfosit plasma biru yang cepat dan murah. Limfosit plasma biru sebagai respon imun dengan persentase 4% di darah tepi pada 98% kasus demam beradarah dengue. Selain peningkatan limfosit plasma biru, infeksi virus dengue juga dapat menyebabkan leukopenia. Leukopenia pada demam berdarah dengue didominasi penurunan jumlah neutrofil yang terjadi pada awal fase kritis (hari ketiga demam). Penelitian ini menggunakan desain cross sectional dengan sampel penelitian 37 pasien anak yang terinfeksi virus dengue di RS Advent Manado, RS TNI AD Wolter Mongisidi, RS Pancaran Kasih Manado pada bulan Desember 2014 sampai Januari 2015. Kriteria masukan adalah Anak usia kurang dari 15 tahun, hari ketiga sampai ketujuh demam, NS1 atau IgG/IgM positif, Orangtua atau wali bersedia menandatangani informed consent. Simpulan: Pada pasien anak dengan infeksi virus dengue yang dilakukan pemeriksaan darah rutin ditemukan adanya leukopenia dengan jumlah leukosit dibawah 4000/mm3.Kata kunci: infeksi virus dengue, leukosit, limfosit plasma biru

scholarly journals CORRELATION OF DENGUE VIRUS SEROTYPE AND DVI SEVERITY IN ADULT PATIENTS

2018 ◽  
Vol 24 (2) ◽  
pp. 185
Author(s):  
Suci Andriani ◽  
Aryati Aryati ◽  
Usman Hadi
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Real Time ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Adult Patients ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
Virus Serotype ◽  
Degree Of Severity

The clinical manifestation of dengue virus infection is often not clear, varies widely from mild to severe. Exposure of dengue virus which serotype is different from a previous infection is a risk factor for the severe manifestation of dengue virus infection. Dengue hemorrhagic fever is classified into four degrees of severity based on clinical manifestations and laboratory results. Real-time RT-PCR Dengue can detect dengue virus serotype in early dengue virus infection. The aimed of this study was to prove the correlation between dengue virus serotype and degree of severity in adult patients. This study was a cross-sectional observational design done in February until July 2016. Subjects consisted of 100 dengue virus infection patients. Serum of the patients was examined using Real-time RT-PCR Dengue (Simplexa™ Dengue). It was shown that from 46 patients with DENV-3 serotype was 63%, DENV-2 serotype 17.4%, DENV-1 serotypes 17.4% and mixed infection of DENV-1 and DENV-3 serotype 2.2%. There was not any DENV-4 serotype. Dengue Hemorrhagic Fever (DHF) stage I was 47.8%, DHF stage II was 30.4%, DHF stage III was 10.9% and Dengue Fever was 10.9%. There was not any DHF stage IV. There was not enough evidence that DENV-3 correlated with the degree of severity (p= 0.510). Based on this research, DENV-3 serotype was the dominant serotype prevalent at the Dr. Soetomo Hospital. There was no correlation between viral dengue serotype and severity in dengue adult patients in this study. 

scholarly journals UPDATE MANAGEMENT OF DENGUE COMPLICATION IN PEDIATRIC

2015 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Soegeng Soegijanto
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Performance ◽  
Kidney Injury ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Liver Involvement ◽  
Dengue Virus Infection ◽  
Important Health ◽  
Grade Iii

Dengue virus infection is one of the important health problems in Indonesia, although the mortality rate has been decreased but many dengue shock syndrome cases is very difficult to be solving handled. It might be due to nature course of dengue virus infection is very difficult to predict of the earlier time of severity occur. THE AIM To get idea to make update management of dengue complication in pediatric. MATERIAL AND METHOD Data were compiled from Dr. Soetomo Hospital Surabaya in 2009. The diagnosis of all cases was based on criteria WHO 1997 and PCR examination in Institute Tropical Disease for identified serotype of dengue virus infection. The unusual cases of dengue virus infection were treated following the new WHO protocol in 2009. RESULT There were only 3 cases with serotype DEN 1, consisted 2 cases had age 1–4 years and 1 had age 5–14 years. 2 cases showed a severe clinical performance as dengue shock syndrome and 1 case showed as unusual case of dengue virus infection. Three report cases of: a. Dengue hemorrhagic fever grade III which liver involvement and had bilateral pleural effusion; b. Dengue hemorrhagic grade III with liver involvement and encephalopathy; c. Dengue hemorrhagic grade III with liver involvement acute kidney injury, myocardial involvement and encephalopathy. All the patients were treated according to new edition WHO protocol and all of the involving organ recovered along with the improvement of the disease. CONCLUSION Update management of dengue complication pediatric should be learned carefully used for helping unusual cases of dengue virus infection.

scholarly journals Roles for Endothelial Cells in Dengue Virus Infection

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Nadine A. Dalrymple ◽  
Erich R. Mackow
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Capillary Permeability ◽  
Virus Disease ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Dengue Virus Infection ◽  
Barrier Functions ◽  
Dengue Disease ◽  
Primary Fluid

Dengue viruses cause two severe diseases that alter vascular fluid barrier functions, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The endothelium is the primary fluid barrier of the vasculature and ultimately the effects of dengue virus infection that cause capillary leakage impact endothelial cell (EC) barrier functions. The ability of dengue virus to infect the endothelium provides a direct means for dengue to alter capillary permeability, permit virus replication, and induce responses that recruit immune cells to the endothelium. Recent studies focused on dengue virus infection of primary ECs have demonstrated that ECs are efficiently infected, rapidly produce viral progeny, and elicit immune enhancing cytokine responses that may contribute to pathogenesis. Furthermore, infected ECs have also been implicated in enhancing viremia and immunopathogenesis within murine dengue disease models. Thus dengue-infected ECs have the potential to directly contribute to immune enhancement, capillary permeability, viremia, and immune targeting of the endothelium. These effects implicate responses of the infected endothelium in dengue pathogenesis and rationalize therapeutic targeting of the endothelium and EC responses as a means of reducing the severity of dengue virus disease.

scholarly journals Protective Capacity of the Human Anamnestic Antibody Response during Acute Dengue Virus Infection

2016 ◽  
Vol 90 (24) ◽  
pp. 11122-11131 ◽  
Author(s):  
Meihui Xu ◽  
Roland Züst ◽  
Ying Xiu Toh ◽  
Jennifer M. Pfaff ◽  
Kristen M. Kahle ◽  
...  
Keyword(s):  
Virus Infection ◽  
Antibody Response ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Immune Memory ◽  
Protective Capacity ◽  
Dengue Virus Infection ◽  
Immune Correlates

ABSTRACT Half of the world's population is exposed to the risk of dengue virus infection. Although a vaccine for dengue virus is now available in a few countries, its reported overall efficacy of about 60% is not ideal. Protective immune correlates following natural dengue virus infection remain undefined, which makes it difficult to predict the efficacy of new vaccines. In this study, we address the protective capacity of dengue virus-specific antibodies that are produced by plasmablasts a few days after natural secondary infection. Among a panel of 18 dengue virus-reactive human monoclonal antibodies, four groups of antibodies were identified based on their binding properties. While antibodies targeting the fusion loop of the glycoprotein of dengue virus dominated the antibody response, two smaller groups of antibodies bound to previously undescribed epitopes in domain II of the E protein. The latter, largely serotype-cross-reactive antibodies, demonstrated increased stability of binding at pH 5. These antibodies possessed weak to moderate neutralization capacity in vitro but were the most efficacious in promoting the survival of infected mice. Our data suggest that the cross-reactive anamnestic antibody response has a protective capacity despite moderate neutralization in vitro and a moderate decrease of viremia in vivo . IMPORTANCE Antibodies can protect from symptomatic dengue virus infection. However, it is not easy to assess which classes of antibodies provide protection because in vitro assays are not always predictive of in vivo protection. During a repeat infection, dengue virus-specific immune memory cells are reactivated and large amounts of antibodies are produced. By studying antibodies cloned from patients with heterologous secondary infection, we tested the protective value of the serotype-cross-reactive “recall” or “anamnestic” response. We found that results from in vitro neutralization assays did not always correlate with the ability of the antibodies to reduce viremia in a mouse model. In addition, a decrease of viremia in mice did not necessarily improve survival. The most protective antibodies were stable at pH 5, suggesting that antibody binding in the endosomes, after the antibody-virus complex is internalized, might be important to block virus spread in the organism.

Effects of Ascogregarina culicis (Eugregarinorida: Lecudinidae) on Mosquito Size and Dengue Virus Infection in Aedes aegypti (Diptera: Culicidae)

2020 ◽  
Author(s):  
Panpim Thongsripong ◽  
Dawn M Wesson
Keyword(s):  
Virus Infection ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infection Rate ◽  
Wing Length ◽  
The Body ◽  
Global Public Health ◽  
Dengue Virus Infection ◽  
Significant Difference ◽  
The Impact

Abstract Dengue virus infection, transmitted via mosquito bites, poses a substantial risk to global public health. Studies suggest that the mosquito’s microbial community can profoundly influence vector-borne pathogen transmissions, including dengue virus. Ascogregarina culicis (Ross) of the phylum Apicomplexa is among the most common parasites of Aedes aegypti (Linnaeus), the principal vector of dengue. Despite a high prevalence worldwide, including in the areas where dengue is endemic, the impact of A. culicis on Ae. aegypti vector competence for dengue virus is unknown. This study aimed to investigate the effects of A. culicis infection on mosquito size and fitness, as measured by wing length, and the susceptibility to dengue virus infection in Ae. aegypti. Our results showed that there was no statistically significant difference in wing lengths between Ae. aegypti infected and not infected with A. culicis. Furthermore, A. culicis infection did not significantly affect dengue virus infection or disseminated infection rate. However, there was a significant association between shorter wings and higher dengue virus infection rate, whereby a 0.1-mm increase in wing length decreased the odds of the mosquito being infected by 32%. Thus, based on our result, A. culicis infection does not influence the body size and dengue virus infection in Ae. aegypti. This study helps to shed light on a common but neglected eukaryotic mosquito parasite.

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