IMMORTALIZATION OF RABBIT ARTICULAR CHONDROCYTES BY SV40 LARGE T ANTIGEN UNDER THE CONTROL OF TYPE II COLLAGEN REGULATORY SEQUENCES: PHENOTYPE STUDY OF THE CHONDROCYTES CULTURED IN 3 DIMENSIONS

1996 ◽  
Vol 88 (1-2) ◽  
pp. 74-74
Author(s):  
Nathalie STEIMBERG ◽  
Sophie THENET-GAUCI ◽  
Monique ADOLPHE
Keyword(s):  
Articular Chondrocytes ◽  
Type Ii Collagen ◽  
T Antigen ◽  
Regulatory Sequences ◽  
Type Ii ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
Rabbit Articular Chondrocytes

scholarly journals Microfilament modification by dihydrocytochalasin B causes retinoic acid-modulated chondrocytes to reexpress the differentiated collagen phenotype without a change in shape.

1988 ◽  
Vol 106 (1) ◽  
pp. 161-170 ◽  
Author(s):  
P D Benya ◽  
P D Brown ◽  
S R Padilla
Keyword(s):  
Retinoic Acid ◽  
Cell Shape ◽  
Articular Chondrocytes ◽  
Type Ii Collagen ◽  
Phenotypic Change ◽  
Type Iii Collagen ◽  
Type I ◽  
Type Ii ◽  
The Stability ◽  
Rabbit Articular Chondrocytes

Primary monolayers of rabbit articular chondrocytes synthesize high levels of type II collagen and proteoglycan. This capacity was used as a marker for the expression of the differentiated phenotype. Such cells were treated with 1 microgram/ml retinoic acid (RA) for 10 d to produce a modulated collagen phenotype devoid of type II and consisting of predominantly type I trimer and type III collagen. After transfer to secondary culture in the presence of RA, the stability of the RA-modulated phenotype was investigated by culture in the absence of RA. Little reexpression of type II collagen synthesis occurred in this period unless cultures were treated with 3 X 10(-6) M dihydrocytochalasin B to modify microfilament structures. Reexpression of the differentiated phenotype began between days 6-8 and was essentially complete by day 14. Substantial reexpression occurred by day 8 without a detectable increase in cell rounding. Colony formation, characteristic of primary chondrocytes, was infrequent even after reexpression was complete. These data suggest that the integrity of microfilament cytoskeletal structures can be a source of regulatory signals that mechanistically appear to be more proximal to phenotypic change than the overt changes in cell shape that accompany reexpression of subculture-modulated chondrocytes in agarose culture.

Fucoidan from Undaria pinnatifida regulates type II collagen and COX-2 expression via MAPK and PI3K pathways in rabbit articular chondrocytes

Biologia ◽  
2017 ◽  
Vol 72 (11) ◽  
Author(s):  
Abdul Rehman Phull ◽  
Song Ja Kim
Keyword(s):  
Articular Chondrocytes ◽  
Undaria Pinnatifida ◽  
Brown Seaweed ◽  
Type Ii Collagen ◽  
Sulfated Polysaccharide ◽  
Type Ii ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Cox 2 ◽  
Rabbit Articular Chondrocytes

AbstractFucoidan is a sulfated polysaccharide widely distributed in brown seaweed. It exhibits several bioactivities, such as anti-cancer, anti-tumor, anti-microbial, anti-diabetic and anti-oxidant properties. However, the effects of fucoidan in chondrocytes are not well established. Previously, we have reported

SV40 large T antigen immortalization of human articular chondrocytes

1995 ◽  
Vol 31 (3) ◽  
pp. 174-177 ◽  
Author(s):  
Bénédicte Benoit ◽  
Sophie Thenet-Gauci ◽  
Françoise Hoffschir ◽  
Patrice Penfornis ◽  
Sylvie Demignot ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
T Antigen ◽  
Human Articular Chondrocytes ◽  
Large T Antigen ◽  
Sv40 Large T Antigen
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