scholarly journals Effect of ethanol extract of HPRS, a Traditional Chinese Medicine formula, on HCT116 cell Line

2016 ◽  
Vol 36 (6) ◽  
pp. 760-767 ◽  
Author(s):  
Lu Jun ◽  
Xu Jun ◽  
Shi Qin
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Cell Line ◽  
Hct116 Cell ◽  
Ethanol Extract ◽  
Hct116 Cell Line ◽  
Chinese Medicine Formula

Fabrication of a monolithic adsorbent with multi-sites and its application in the extraction of active components from traditional Chinese medicine formula

2021 ◽  
pp. 106591
Author(s):  
Fanrong Sun ◽  
Laisen Wang ◽  
Changqing Yu ◽  
Haiyan Liu ◽  
Hongyuan Yan ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Active Components ◽  
Chinese Medicine Formula

scholarly journals Regulation of Transforming Growth Factor α Expression in a Growth Factor-Independent Cell Line

1998 ◽  
Vol 18 (1) ◽  
pp. 303-313 ◽  
Author(s):  
Gillian M. Howell ◽  
Lisa E. Humphrey ◽  
Barry L. Ziober ◽  
Rana Awwad ◽  
Basker Periyasamy ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Antisense Rna ◽  
Promoter Activity ◽  
Transforming Growth Factor ◽  
Hct116 Cell ◽  
Malignant Phenotype ◽  
Hct116 Cell Line ◽  
Transforming Growth Factor Α ◽  
Factor Α

ABSTRACT Aberrant transcriptional regulation of transforming growth factor α (TGFα) appears to be an important contributor to the malignant phenotype and the growth factor independence with which malignancy is frequently associated. However, little is known about the molecular mechanisms responsible for dysregulation of TGFα expression in the malignant phenotype. In this paper, we report on TGFα promoter regulation in the highly malignant growth factor-independent cell line HCT116. The HCT116 cell line expresses TGFα and the epidermal growth factor receptor (EGFR) but is not growth inhibited by antibodies to EGFR or TGFα. However, constitutive expression of TGFα antisense RNA in the HCT116 cell line resulted in the isolation of clones with markedly reduced TGFα mRNA and which were dependent on exogenous growth factors for proliferation. We hypothesized that if TGFα autocrine activation is the major stimulator of TGFα expression in this cell line, TGFα promoter activity should be reduced in the antisense TGFα clones in the absence of exogenous growth factor. This was the case. Moreover, transcriptional activation of the TGFα promoter was restored in an antisense-TGFα-mRNA-expressing clone which had reverted to a growth factor-independent phenotype. Using this model system, we were able to identify a 25-bp element within the TGFα promoter which conferred TGFα autoregulation to the TGFα promoter in the HCT116 cell line. In the TGFα-antisense-RNA-expressing clones, this element was activated by exogenous EGF. This 25-bp sequence contained no consensus sequences of known transcription factors so that the TGFα or EGF regulatory element within this 25-bp sequence represents a unique element. Further characterization of this 25-bp DNA sequence by deletion analysis revealed that regulation of TGFα promoter activity by this sequence is complex, as both repressors and activators bind in this region, but the overall expression of the activators is pivotal in determining the level of response to EGF or TGFα stimulation. The specific nuclear proteins binding to this region are also regulated in an autocrine-TGFα-dependent fashion and by exogenous EGF in EGF-deprived TGFα antisense clone 33. This regulation is identical to that seen in the growth factor-dependent cell line FET, which requires exogenous EGF for optimal growth. Moreover, the time response of the stimulation oftrans-acting factor binding by EGF suggests that the effect is directly due to growth factor and not mediated by changes in growth state. We conclude that this element appears to represent the major positive regulator of TGFα expression in the growth factor-independent HCT116 cell line and may represent the major site of transcriptional dysregulation of TGFα promoter activity in the growth factor-independent phenotype.

scholarly journals Clinical Therapeutic Effects of Aspirin in Combination with Fufang Danshen Diwan, a Traditional Chinese Medicine Formula, on Coronary Heart Disease: A Systematic Review and Meta-Analysis

2016 ◽  
Vol 39 (5) ◽  
pp. 1955-1963 ◽  
Author(s):  
Jianchun Huang ◽  
Xiaojun Tang ◽  
Fangxing Ye ◽  
Junhui He ◽  
Xiaolong Kong
Keyword(s):  
Systematic Review ◽  
Coronary Heart Disease ◽  
Heart Disease ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Randomized Clinical Trials ◽  
Meta Analysis ◽  
Aspirin Therapy ◽  
Therapeutic Effects ◽  
Chinese Medicine Formula

Background/Aims: Coronary heart disease is characterized by vascular stenosis or occlusion resulting in myocardial ischemia, hypoxia and necrosis. In China, the combination of aspirin and Fufang Danshen Diwan (FDD), a traditional Chinese medicine formula, has been suggested in the treatment of coronary heart disease. There have been several studies comparing the effectiveness of aspirin alone and in combination with FDD to treat coronary artery disease; however, it remains unclear whether combined aspirin therapy is superior. This study was thus designed to clarify this issue through a systematic review and meta-analysis. Methods: Databases including PubMed, EMBASE, China National Knowledge Infrastructure (CNKI) database, Wanfang Data and VIP Information were searched. Papers were reviewed systematically by two researchers and analyzed using Cochrane software Revman 5.1. Results: Fourteen randomized controlled trials enrolling 1367 subjects were included. Meta-analyses revealed that aspirin in combination with FDD was significantly more effective at alleviating angina pectoris and improving electrocardiogram (ECG) results relative to aspirin therapy alone, reflected by the summary effects for the clinical markedly effective (OR = 2.45; 95% CI 1.95-3.08) and the total effective (OR = 3.92; 95% CI 2.87-5.36) rates. In addition, combined aspirin and FDD was significantly more efficacious than aspirin monotherapy at improving blood lipid levels, as indicated by the following outcomes: 1) reduction of TC level (SMD −1.12; 95% CI −1.49 to −0.76); 2) reduction of TG level (SMD −0.94; 95% CI −1.15 to -0.74); 3) reduction of LDL level (SMD -0.68; 95% CI -0.88 to -0.48); and 4) improvement of HDL level (SMD 0.52; 95% CI 0.04 to 0.99 ). No serious adverse events were reported in any of the included trials. Conclusion: The present meta-analysis demonstrated that aspirin in combination with FDD was more effective than aspirin alone for treating coronary heart disease. More full-scale randomized clinical trials with reliable designs are recommended to further evaluate the clinical benefits and long-term effectiveness of FDD for the treatment of coronary heart disease.

scholarly journals Flavonoid profiling of a traditional Chinese medicine formula of Huangqin Tang using high performance liquid chromatography

2016 ◽  
Vol 6 (2) ◽  
pp. 148-157 ◽  
Author(s):  
Tao Li ◽  
Shuaixing Zhuang ◽  
Yiwei Wang ◽  
Yanli Wang ◽  
Weihao Wang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
High Performance ◽  
Chinese Medicine Formula

The Combination of Aricept with a Traditional Chinese Medicine Formula, Smart Soup, May Be a Novel Way to Treat Alzheimer's Disease

2015 ◽  
Vol 45 (4) ◽  
pp. 1185-1195 ◽  
Author(s):  
Ying Wang ◽  
Ying Wang ◽  
Yi Sui ◽  
Hongshuang Yu ◽  
Xiaoheng Shen ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Chinese Medicine Formula

scholarly journals Jianpi Jiedu decoction, a traditional Chinese medicine formula, inhibits tumorigenesis, metastasis, and angiogenesis through the mTOR/HIF-1α/VEGF pathway

2018 ◽  
Vol 224 ◽  
pp. 140-148 ◽  
Author(s):  
Weijun Peng ◽  
Shaofang Zhang ◽  
Zheyu Zhang ◽  
Panpan Xu ◽  
Dan Mao ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Chinese Medicine Formula ◽  
Vegf Pathway

Assessment of the Anticancer and Antigenotoxic potential of Costus speciosus extract against HCT116 cell line

2017 ◽  
Vol 29 (1) ◽  
Author(s):  
Eman Althubaiti ◽  
Abdulkader Shaikh Omar ◽  
Mohammed Almatry ◽  
Yasir Anwar ◽  
Soufyan ElAssouli
Keyword(s):  
Cell Line ◽  
Hct116 Cell ◽  
Hct116 Cell Line ◽  
Costus Speciosus
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