scholarly journals Evaluation of Glucose-Methylene-Blue-Mueller-Hinton Agar for E-Test Minimum Inhibitory Concentration Determination in Candida spp.

2007 ◽  
Vol 25 (4) ◽  
pp. 432-433
Author(s):  
MR Capoor ◽  
D Rawat ◽  
D Nair ◽  
M Deb ◽  
P Aggarwal
Keyword(s):  
Methylene Blue ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Candida Spp ◽  
Concentration Determination ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

scholarly journals Disk Diffusion-Based Methods for Determining Candida parapsilosis Susceptibility to Anidulafungin

2003 ◽  
Vol 47 (9) ◽  
pp. 3018-3020 ◽  
Author(s):  
Zekaver Odabasi ◽  
Victor Paetznick ◽  
Beth P. Goldstein ◽  
John H. Rex ◽  
Luis Ostrosky-Zeichner
Keyword(s):  
Methylene Blue ◽  
Dimethyl Sulfoxide ◽  
Candida Parapsilosis ◽  
Tween 80 ◽  
Disk Diffusion ◽  
Broth Microdilution ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

ABSTRACT Zone diameters for anidulafungin by disk diffusion for 139 isolates of C. parapsilosis were compared with MICs by NCCLS M27-A2 broth microdilution. The comparison was poor unless the disks were prepared by dissolving anidulafungin in 1% dimethyl sulfoxide plus 0.1% Tween 80 and testing on Mueller-Hinton agar flooded with glucose and methylene blue.

Assembly and antifungal effect of a new fluconazole-carrier nanosystem

2020 ◽  
Vol 15 (4) ◽  
pp. 273-285 ◽  
Author(s):  
Taynara MT de Lima ◽  
Laís S Arias ◽  
Letícia F Afanaci ◽  
Raphael FB Ferraresse ◽  
Francisco N de S Neto ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Candida Glabrata ◽  
Inhibitory Concentration ◽  
Fungal Infections ◽  
Oxide Nanoparticles ◽  
Total Biomass ◽  
Planktonic Cells ◽  
Antifungal Effect ◽  
Concentration Determination ◽  
Antifungal Effects

Aim: To assemble, characterize and assess the antifungal effects of a new fluconazole (FLZ)-carrier nanosystem. Materials & methods: The nanosystem was prepared by loading FLZ on chitosan (CS)-coated iron oxide nanoparticles (IONPs). Antifungal effects were evaluated on planktonic cells (by minimum inhibitory concentration determination) and on biofilms (by quantification of cultivable cells, total biomass, metabolism and extracellular matrix) of Candida albicans and Candida glabrata. Results: Characterization results ratified the formation of a nanosystem (<320 nm) with FLZ successfully embedded. IONPs-CS-FLZ nanosystem reduced minimum inhibitory concentration values and, in general, showed similar antibiofilm effects compared with FLZ alone. Conclusion: IONPs-CS-FLZ nanosystem was more effective than FLZ mainly in inhibiting Candida planktonic cells. This nanocarrier has potential to fight fungal infections.

scholarly journals Attempts to Access a Series of Pyrazoles Lead to New Hydrazones with Antifungal Potential Against Candida Species Including Azole-Resistant Strains

2021 ◽  
Author(s):  
Georgiana NEGRU ◽  
Laure KAMUS ◽  
Elena Bîcu ◽  
Sergiu Shova ◽  
Boualem SENDID ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Agents ◽  
Inhibitory Concentration ◽  
Pyrazole Derivatives ◽  
Mammalian Cell Line ◽  
Cell Toxicity ◽  
Candida Spp ◽  
X Ray ◽  
Antifungal Potential ◽  
Resistant Strains

The treatment of benzylidenemalononitriles 3a-c with phenylhydrazines 4a-n in refluxing ethanol did not provide pyrazole derivatives but furnished hydrazones 1a-o. The structure of hydrazones was secured by X-Ray analysis. Newly synthesized hydrazones 1a-o were tested against 8 Candida spp. strains in a dose response assay to determine the minimum inhibitory concentration (MIC99). Five compounds 1c, 1d, 1i, 1k and 1l were identified as promising antifungal agents against Candida spp. (C. albicans SC5314, C. glabrata, C. tropicalis, C. parapsilosis and C. glabrata (R azoles)) with MIC99 values ranging from 16 to 32 &micro;g/mL. To further evaluate the antifungal potential of the active compounds, they have been assayed against a mammalian cell line HEK293 to determine general cell toxicity and on NCI-60 cancer cell lines panel, demonstrating selectivity antifungal activity over cytotoxicity.

scholarly journals Disk diffusion Method in Enriched Mueller Hinton agar for determining susceptibility of Candida isolates from various clinical specimens

2020 ◽  
Vol 28 (1) ◽  
pp. 28-33
Author(s):  
Nabeela Mahboob ◽  
Hasina Iqbal ◽  
Mushtaque Ahmed ◽  
Md Mehedi Hasan Magnet ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Methylene Blue ◽  
Amphotericin B ◽  
Candida Species ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Disk Diffusion ◽  
Clinical Specimens ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Background: Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threatening invasive diseases. Recently there is a serious concern with increased resistance of antifungal drugs and its consequences. Thus, identification of Candida and its antifungal susceptibility testing has a paramount significance in the management of Candidal infections. The aim of the study was to determine antifungal susceptibility pattern of Candida by Mueller-Hinton agar media supplemented with glucose and methylene blue for disk diffusion testing of fluconazole, miconazole, clotrimazole, amphotericin B and nystatin. Methods: A total of 35 Candida species was isolated from 2000 clinical specimens over 6 month’s period from July 2016 to December 2016. Growths on Blood agar and chromogenic agar were evaluated for colony appearance and microscopic examination. Antifungal susceptibility testing was performed by disk diffusion using Mueller-Hinton agar supplemented with glucose and methylene blue. Results: Candida species were more sensitive to clotrimazole (88.58%) and amphotericin B (88.58%) followed by nystatin ((77.14%), miconazole (74.29%) whereas fluconazole showed the highest level of resistance (60%). Conclusions: The increase in resistance to fluconazole is of serious concern as it is the most commonly used azole for candidiasis. The sensitivity profile of Candida isolates will be helpful to choose appropriate antifungal agents, thus decreasing patient’s morbidity and mortality. J Dhaka Medical College, Vol. 28, No.1, April, 2019, Page 28-33

scholarly journals Comparison of Automated and Traditional Minimum Inhibitory Concentration Procedures for Microbiological Cosmetic Preservatives

1996 ◽  
Vol 79 (6) ◽  
pp. 1294-1299 ◽  
Author(s):  
Melissa E Lenczewski ◽  
Sean T McGavin ◽  
Karl Vandyke
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Color Change ◽  
Gram Negative Bacteria ◽  
Cosmetic Products ◽  
Mueller Hinton ◽  
Visual Color ◽  
Redox Indicator ◽  
Tetrazolium Violet ◽  
Mueller Hinton Broth

Abstract Minimum inhibitory concentration (MIC) is used to test resistance of microorganisms against antibiotics and to test cosmetic preservatives. This research expanded traditional MIC with automation and application of colorimetric endpoint MIC. All experiments included common cosmetic preservatives and microorganisms used in testing preservative efficacy. An autodilutor using three 96-well microliter plates processed 6 preservatives against 1 microorganism in 15 min. The unique tip design made it possible to accurately deliver viscous test materials that cannot be dispensed accurately with vacuum or fluid-filled systems. Tetrazolium violet, a redox indicator, provided a visual color change from clear to purple at the MIC. Optimum concentration of tetrazolium violet was 0.01 % with addition of 0.2% glucose to Mueller-Hinton broth for both gram-positive and gram-negative bacteria. The colorimetric endpoint was evident after 24 h from previously cryogenically stored organisms that were thawed before use and after 4 h for 18–24 h broth cultures subcultured from agar plates. The autodilutor accurately pipetted viscous cosmetic products such as hand lotion and shampoo, which cannot be pipetted with a traditional micropipetter.

scholarly journals Antimicrobial Activity of Some Derivatives of 1,4-Dihydropyridines

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Prabha Mehta ◽  
Prabha Verma
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Aromatic Aldehyde ◽  
Multicomponent Reaction ◽  
Inhibitory Concentration ◽  
Ammonium Hydroxide ◽  
Pharmacological Properties ◽  
Mueller Hinton ◽  
Derivatives Of ◽  
Mueller Hinton Broth

Hantzsch reported the synthesis of functionalized 1,4-dihydropyridines via three-component condensation of an aromatic aldehyde, ketoester, and ammonium hydroxide. This multicomponent reaction is of much importance due to excellent pharmacological properties of dihydropyridines. In this account, we synthesized some halo- and nitrophenyl dihydropyridines and evaluated their antimicrobial activity. The minimum inhibitory concentration (MIC) was determined by microdilution technique in Mueller Hinton broth. The MICs were recorded after 24 hours of incubation at 37°C. These results showed that these compounds exhibited significant to moderate activities against both Gram-(+) and Gram-(−) organisms.

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