Survival and neurite outgrowth of rat cortical neurons in three-dimensional agarose and collagen gel matrices

The spatial distribution of neurons and activity-dependent neurite outgrowth shape long-range interaction, recurrent local connectivity and the modularity in neuronal networks. We investigated how this mesoscale architecture develops by interaction of neurite outgrowth, cell migration and activity in cultured networks of rat cortical neurons and show that simple rules can explain variations of network modularity. In contrast to theoretical studies on activity-dependent outgrowth but consistent with predictions for modular networks, spontaneous activity and the rate of synchronized bursts increased with clustering, whereas peak firing rates in bursts increased in highly interconnected homogeneous networks. As Ca2+ influx increased exponentially with increasing network recruitment during bursts, its modulation was highly correlated to peak firing rates. During network maturation, long-term estimates of Ca2+ influx showed convergence, even for highly different mesoscale architectures, neurite extent, connectivity, modularity and average activity levels, indicating homeostatic regulation towards a common set-point of Ca2+ influx.

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Effects of docosahexaenoic acid on the survival and neurite outgrowth of rat cortical neurons in primary cultures

The Journal of Nutritional Biochemistry ◽

10.1016/j.jnutbio.2005.02.002 ◽

2005 ◽

Vol 16 (9) ◽

pp. 538-546 ◽

Cited By ~ 66

Author(s):

Dehua Cao ◽

Renhao Xue ◽

Jun Xu ◽

Zhili Liu

Keyword(s):

Docosahexaenoic Acid ◽

Neurite Outgrowth ◽

Cortical Neurons ◽

Primary Cultures ◽

Rat Cortical Neurons

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The Protective Effects of Jatrorrhizine on β-Amyloid (25-35)-Induced Neurotoxicity in Rat Cortical Neurons

CNS & Neurological Disorders - Drug Targets ◽

10.2174/1871527311211080013 ◽

2013 ◽

Vol 11 (8) ◽

pp. 1030-1037 ◽

Cited By ~ 17

Author(s):

Tao Luo ◽

Wei Jiang ◽

Yan Kong ◽

Sheng Li ◽

Feng He ◽

...

Keyword(s):

Cortical Neurons ◽

Protective Effects ◽

Rat Cortical Neurons ◽

Β Amyloid

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Targeting hepatocyte growth factor in epithelial–stromal interactions in an in vitro experimental model of human periodontitis

Odontology ◽

10.1007/s10266-021-00625-0 ◽

2021 ◽

Author(s):

Yoko Yamaguchi ◽

Akira Saito ◽

Masafumi Horie ◽

Akira Aoki ◽

Patrick Micke ◽

...

Keyword(s):

Growth Factor ◽

Hepatocyte Growth Factor ◽

Experimental Studies ◽

Three Dimensional ◽

Collagen Gel ◽

Neutralizing Antibody ◽

Chronic Inflammatory Disease ◽

Collagen Degradation ◽

Hepatocyte Growth

AbstractPeriodontitis is a chronic inflammatory disease leading to progressive connective tissue degradation and loss of the tooth-supporting bone. Clinical and experimental studies suggest that hepatocyte growth factor (HGF) is involved in the dysregulated fibroblast–epithelial cell interactions in periodontitis. The aim of this study was to explore effects of HGF to impact fibroblast-induced collagen degradation. A patient-derived experimental cell culture model of periodontitis was applied. Primary human epithelial cells and fibroblasts isolated from periodontitis-affected gingiva were co-cultured in a three-dimensional collagen gel. The effects of HGF neutralizing antibody on collagen gel degradation were tested and transcriptome analyses were performed. HGF neutralizing antibody attenuated collagen degradation and elicited expression changes of genes related to extracellular matrix (ECM) and cell adhesion, indicating that HGF signaling inhibition leads to extensive impact on cell–cell and cell–ECM interactions. Our study highlights a potential role of HGF in periodontitis. Antagonizing HGF signaling by a neutralizing antibody may represent a novel approach for periodontitis treatment.

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Extending In-Plane Impedance Measurements from 2D to 3D Cultures: Design Considerations

Bioengineering ◽

10.3390/bioengineering8010011 ◽

2021 ◽

Vol 8 (1) ◽

pp. 11

Author(s):

Sorel E. De Leon ◽

Lana Cleuren ◽

Zay Yar Oo ◽

Paul R. Stoddart ◽

Sally L. McArthur

Keyword(s):

Three Dimensional ◽

3D Culture ◽

Collagen Gel ◽

Impedance Measurement ◽

Impedance Spectrum ◽

3D Models ◽

Low Frequencies ◽

3D Cell Cultures ◽

3D Collagen ◽

2D And 3D

Three-dimensional (3D) cell cultures have recently emerged as tools for biologically modelling the human body. As 3D models make their way into laboratories there is a need to develop characterisation techniques that are sensitive enough to monitor the cells in real time and without the need for chemical labels. Impedance spectroscopy has been shown to address both of these challenges, but there has been little research into the full impedance spectrum and how the different components of the system affect the impedance signal. Here we investigate the impedance of human fibroblast cells in 2D and 3D collagen gel cultures across a broad range of frequencies (10 Hz to 5 MHz) using a commercial well with in-plane electrodes. At low frequencies in both 2D and 3D models it was observed that protein adsorption influences the magnitude of the impedance for the cell-free samples. This effect was eliminated once cells were introduced to the systems. Cell proliferation could be monitored in 2D at intermediate frequencies (30 kHz). However, the in-plane electrodes were unable to detect any changes in the impedance at any frequency when the cells were cultured in the 3D collagen gel. The results suggest that in designing impedance measurement devices, both the nature and distribution of the cells within the 3D culture as well as the architecture of the electrodes are key variables.

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