Erythropoietin restores glutathione peroxidase activity in 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine-induced neurotoxicity in C57BL mice and stimulates murine astroglial glutathione peroxidase production in vitro

2002 ◽  
Vol 321 (1-2) ◽  
pp. 73-76 ◽  
Author(s):  
Sermin Genc ◽  
Mustafa Akhisaroglu ◽  
Filiz Kuralay ◽  
Kursad Genc
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Glutathione Peroxidase Activity ◽  
C57bl Mice

Ascorbate interacts with sodium selenite to increase glutathione peroxidase activity in selenium-deficient chick duodena cultured in vitro

1989 ◽  
Vol 20 (1-2) ◽  
pp. 87-94 ◽  
Author(s):  
Mary S. Cupp ◽  
Gerald F. Combs ◽  
Robert A. Corradino
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Sodium Selenite ◽  
Glutathione Peroxidase Activity

Increased glutathione peroxidase activity in human blood mononuclear cells upon in vitro incubation with n-3 fatty acids

1994 ◽  
Vol 47 (8) ◽  
pp. 1315-1323 ◽  
Author(s):  
Catherine Joulain ◽  
Annie F. Prigent ◽  
Georges Némoz ◽  
Michel Lagarde
Keyword(s):  
Fatty Acids ◽  
Glutathione Peroxidase ◽  
Human Blood ◽  
Peroxidase Activity ◽  
Mononuclear Cells ◽  
Glutathione Peroxidase Activity ◽  
In Vitro Incubation ◽  
Blood Mononuclear Cells

Vitamin E protection of obesity-enhanced vascular calcification in uremic rats

2014 ◽  
Vol 306 (4) ◽  
pp. F422-F429 ◽  
Author(s):  
A. Peralta-Ramírez ◽  
A. Montes de Oca ◽  
A. I. Raya ◽  
C. Pineda ◽  
I. López ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Calcium Concentration ◽  
Soft Tissues ◽  
Zucker Rats ◽  
Glutathione Peroxidase Activity ◽  
Obese Rats

This study aimed to determine the extent of extraskeletal calcification in uremic Zucker rats, by comparing obese and lean phenotypes, and to evaluate the influence of vitamin E (VitE) on the development of calcifications in both uremic rats and human vascular smooth muscle cells (HVSMCs) cultured in vitro. Zucker rats of lean and obese phenotypes with normal renal function [control (C); C-lean and C-obese groups] and with uremia [5/6 nephrectomy (Nx); Nx-lean and Nx-obese groups] and uremic rats treated with VitE (Nx-lean + VitE and Nx-obese + VitE groups) were studied. Uremic groups were subjected to Nx, fed a 0.9% phosphorus diet, and treated with calcitriol (80 ng/kg ip). The aortic calcium concentration was significantly higher ( P < 0.05) in Nx-obese rats (10.0 ± 2.1 mg/g tissue) than in Nx-lean rats (3.6 ± 1.3 mg/g tissue). A decrease in plasma glutathione peroxidase activity was observed in Nx-obese rats compared with Nx-lean rats (217.2 ± 18.2 vs. 382.3 ± 15.5 nmol·min−1·ml−1, P < 0.05). Treatment with VitE restored glutathione peroxidase activity and reduced the aortic calcium concentration to 4.6 ± 1.3 mg/g tissue. The differences in mineral deposition between Nx-lean, Nx-obese, Nx-lean + VitE, and Nx-obese + VitE rats were also evidenced in other soft tissues. In HVSMCs incubated with high phosphate, VitE also prevented oxidative stress and reduced calcium content, bone alkaline phosphatase, and gene expression of core-binding factor-α1. In conclusion, uremic obese rats develop more severe calcifications than uremic lean rats and VitE reduces oxidative stress and vascular calcifications in both rats and cultures of HVSMCs.

In Vivo and in Vitro Variations of Human Erythrocyte Glutathione Peroxidase Activity as Result of Cells Ageing, Selenium Availability and Peroxide Activation

1978 ◽  
Vol 39 (3) ◽  
pp. 399-408 ◽  
Author(s):  
G. Perona ◽  
G. C. Guidi ◽  
A. Piga ◽  
R. Cellerino ◽  
R. Menna ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Human Erythrocyte ◽  
Glutathione Peroxidase Activity ◽  
Peroxide Activation

Glutathione Peroxidase And Malondialdehyde Production In The Arachidonic Acid Metabolism Of Human Platelets

1981 ◽  
Author(s):  
G C Guidi ◽  
R Schiavon ◽  
G L Avventi ◽  
G Perona
Keyword(s):  
Arachidonic Acid ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Acid Metabolism ◽  
Inverse Correlation ◽  
Human Platelets ◽  
Arachidonic Acid Metabolism ◽  
Glutathione Peroxidase Activity ◽  
Physiological Variations

A series of functional parameters, including the aggregability triggered by various agents, the in vitro malondialdehyde production and the glutathio ne peroxidase activity, has been investigated in platelets from normal blood donors.Glutathione peroxidase activity assays showed a significant inverse correlation with malondialdehyde induced by arachidonic acid but not with aggregation data and malondialdehyde induced by thrombin. Moreover, arachidonic acid generates in human platelets lysates large amounts of hydrogen acceptor substrate(s) for the glutathione peroxidase with peculiar kinetic features. These are related to ma londialdehyde production and to partial inhibition by acetyl-salicilic acid and are likely connected with prostaglandin metabolism.Our data suggest that physiological variations in glutathione peroxidase activity are important in human platelet arachidonic acid metabolism, because they modulate the biosynthesis of key end-products, as thromboxane A2, whose malondialdehyde is an inde.

scholarly journals Two immunologically distinct Yc-type subunits are present in rat lung glutathione S-transferases

1984 ◽  
Vol 224 (1) ◽  
pp. 335-338 ◽  
Author(s):  
S V Singh ◽  
Y C Awasthi
Keyword(s):  
Glutathione Peroxidase ◽  
Rat Liver ◽  
Peroxidase Activity ◽  
Gel Electrophoresis ◽  
Rat Lung ◽  
Glutathione Peroxidase Activity ◽  
Glutathione S Transferase ◽  
Two Dimensional Gel Electrophoresis ◽  
Glutathione S Transferases

Two types of 25 000-Mr subunits are present in rat lung glutathione S-transferase I (pI 8.8). These subunits, designated Yc and Yc', are immunologically and functionally distinct from each other. The homodimers YcYc (pI 10.4) and Yc'Yc' (pI 7.6) obtained by hybridization in vitro of the two subunits of glutathione S-transferase I (pI 8.8) were isolated and characterized. Results of these studies indicate that only the Yc subunits express glutathione peroxidase activity and cross-react with the antibodies raised against glutathione S-transferase B (YaYc) or rat liver. The Yc' subunits do not express glutathione peroxidase activity and do not cross-react with the antibodies raised against glutathione S-transferase B of rat liver. The amino acid compositions of these two subunits are also different. These two subunits can also be separated by the two-dimensional gel electrophoresis of glutathione S-transferase I (pI 8.8) of rat lung.

Glutathione peroxidase activity in seminal plasma and its relationship to classical sperm parameters and in vitro fertilization-intracytoplasmic sperm injection outcome

2012 ◽  
Vol 97 (4) ◽  
pp. 852-857.e1 ◽  
Author(s):  
Lorena Crisol ◽  
Roberto Matorras ◽  
Fermín Aspichueta ◽  
Antonia Expósito ◽  
Maria Luisa Hernández ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Glutathione Peroxidase ◽  
Seminal Plasma ◽  
Peroxidase Activity ◽  
Intracytoplasmic Sperm Injection ◽  
Sperm Parameters ◽  
Glutathione Peroxidase Activity ◽  
Vitro Fertilization

Low Selenium Status in the Elderly Influences Thyroid Hormones

1995 ◽  
Vol 89 (6) ◽  
pp. 637-642 ◽  
Author(s):  
Oliviero Olivieri ◽  
Domenico Girelli ◽  
Margherita Azzini ◽  
Anna Maria Stanzial ◽  
Carla Russo ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Thyroid Hormones ◽  
Peroxidase Activity ◽  
Controlled Trial ◽  
The Elderly ◽  
Serum Selenium ◽  
Selenium Status ◽  
Glutathione Peroxidase Activity ◽  
Double Blind ◽  
Double Blind Placebo

1. Iodothyronine 5′-deiodinase, which is mainly responsible for peripheral triiodothyronine (T3) production, has recently been demonstrated to be a selenium-containing enzyme. In the elderly, reduced peripheral conversion of thyroxine (T4) to T3 and overt hypothyroidism are frequently observed. 2. We measured serum selenium and erythrocyte glutathione peroxidase (as indices of selenium status), thyroid hormones and thyroid-stimulating hormone in 109 healthy euthyroid subjects (52 women, 57 men), carefully selected to exclude abnormally low thyroid hormone levels induced by acute or chronic diseases or calorie restriction. The subjects were subdivided into three age groups. To avoid conditions of undernutrition or malnutrition, dietary records were obtained for a sample of 24 subjects, randomly selected and representative of the whole population for age and sex. 3. In order to properly assess the influence of selenium status on iodothyronine 5′-deiodinase type I activity, a double-blind placebo-controlled trial was also carried out on 36 elderly subjects, resident at a privately owned nursing home. 4. In the free-living population, a progressive reduction of the T3/T4 ratio (due to increased T4 levels) and of selenium and erythrocyte glutathione peroxidase activity was observed with advancing age. A highly significant linear correlation between T4, T3/T4 and selenium was observed in the population as a whole (for T4, R = −0.312, P < 0.002; for T3/T4 ratio, R = 0.32, P < 0.01) and in older subjects (for T4, R = −0.40, P < 0.05; for T3/T4 ratio, R = 0.54, P < 0.002). 5. The main result of the double-blind placebo-controlled trial was a significant improvement of selenium indices and a decrease in the T4 level in selenium-treated subjects; serum selenium, erythrocyte glutathione peroxidase activity and thyroid hormones did not change in placebo-treated subjects. 6. We concluded that selenium status influences thyroid hormones in the elderly, mainly modulating T4 levels.

scholarly journals Selenium levels and glutathione peroxidase activity in patients with ataxia-telangiectasia: association with oxidative stress and lipid status biomarkers

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Itana Gomes Alves Andrade ◽  
Fabíola Isabel Suano-Souza ◽  
Fernando Luiz Affonso Fonseca ◽  
Carolina Sanchez Aranda Lago ◽  
Roseli Oselka Saccardo Sarni
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Oxidized Ldl ◽  
Reference Value ◽  
Glutathione Peroxidase Activity ◽  
Oxidative Stress Biomarkers ◽  
Apo B ◽  
Lipid Status ◽  
And Oxidative Stress

Abstract Introduction Ataxia-Telangiectasia (A-T) is a multi-system disorder that may be associated with endocrine changes, oxidative stress in addition to inflammation. Studies suggest that selenium is a trace element related to protection against damage caused by oxidative stress. Objective To describe the plasma levels of selenium and erythrocyte glutathione peroxidase activity in A-T patients and to relate them to oxidative stress and lipid status biomarkers. Methods This is a cross-sectional and controlled study evaluating 22 A-T patients (age median, 12.2 years old) matched by gender and age with 18 healthy controls. We evaluated: nutritional status, food intake, plasma selenium levels, erythrocyte glutathione peroxidase activity, lipid status, inflammation and oxidative stress biomarkers. Results Adequate levels of selenium were observed in 24/36 (66.7%) in this evaluated population. There was no statistically significant difference between the groups in selenium levels [47.6 μg/L (43.2–57.0) vs 54.6 (45.2–62.6) μg/dL, p = 0.242]. Nine of A-T patients (41%) had selenium levels below the reference value. The A-T group presented higher levels of LDL-c, non-HDL-c, oxidized LDL, Apo B, Apo-B/Apo-A-I1, LDL-c/HDL-c ratio, malondialdehyde [3.8 µg/L vs 2.8 µg/L, p = 0.029] and lower Apo-A-I1/HDL-c and glutathione peroxidase activity [7300 U/L vs 8686 U/L, p = 0.005]. Selenium levels were influenced, in both groups, independently, by the concentrations of oxidized LDL, malonaldehyde and non-HDL-c. The oxidized LDL (AUC = 0.849) and ALT (AUC = 0.854) were the variables that showed the greatest discriminatory power between groups. Conclusion In conclusion, we observed the presence of selenium below the reference value in nearly 40% and low GPx activity in A-T patients. There was a significant, inverse and independent association between selenium concentrations and oxidative stress biomarkers. Those data reinforce the importance of assessing the nutritional status of selenium in those patients.

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