Failure of dogs to shed oocysts after being fed bovine fetuses naturally infected by Neospora caninum

2001 ◽  
Vol 97 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Nadia Bergeron ◽  
Gilles Fecteau ◽  
Alain Villeneuve ◽  
Christiane Girard ◽  
Julie Paré
Keyword(s):  
Neospora Caninum ◽  
Bovine Fetuses

Detection of Neospora caninum DNA in cases of bovine and ovine abortion in the South-West of Scotland

Parasitology ◽  
2019 ◽  
Vol 146 (07) ◽  
pp. 979-982 ◽  
Author(s):  
P. M. Bartley ◽  
S. Guido ◽  
C. Mason ◽  
H. Stevenson ◽  
F. Chianini ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Economic Losses ◽  
The South ◽  
Farming Community ◽  
South West ◽  
Reproductive Losses ◽  
Bovine Fetuses ◽  
Cattle Farming ◽  
Ovine Fetal ◽  
Cattle And Sheep

AbstractNeospora caninum is a commonly diagnosed cause of reproductive losses in farmed ruminants worldwide. This study examined 495 and 308 samples (brain, heart and placenta) which were collected from 455 and 119 aborted cattle and sheep fetuses, respectively. DNA was extracted and a nested Neospora ITS1 PCR was performed on all samples. The results showed that for bovine fetuses 79/449 brain [17.6% (14.2–21.4)], 7/25 heart [28.0% (12.1–49.4)] and 5/21 placenta [23.8% (8.2–47.2)] were PCR positive for the presence of Neospora DNA. Overall 82/455 [18.0% (14.6–21.7)] of the bovine fetuses tested positive for the presence of N. caninum DNA in at least one sample. None (0/308) of the ovine fetal samples tested positive for the presence of Neospora DNA in any of the tissues tested. The results show that N. caninum was associated with fetal losses in cattle (distributed across South-West Scotland), compared to sheep in the same geographical areas where no parasite DNA was found. Neospora is well distributed amongst cattle in South-West Scotland and is the potential cause of serious economic losses to the Scottish cattle farming community; however, it does not appear to be a problem amongst the Scottish sheep flocks.

Immune responses in pregnant cattle and bovine fetuses following experimental infection with Neospora caninum

2001 ◽  
Vol 87 (10) ◽  
pp. 817-825 ◽  
Author(s):  
Andrianarivo A. ◽  
Barr B. ◽  
Anderson M. ◽  
Rowe J. ◽  
Packham A. ◽  
...  
Keyword(s):  
Immune Responses ◽  
Experimental Infection ◽  
Neospora Caninum ◽  
Bovine Fetuses

scholarly journals Neospora caninum in bovine fetuses of Minas Gerais, Brazil: genetic characteristics of rDNA

2011 ◽  
Vol 20 (4) ◽  
pp. 281-288 ◽  
Author(s):  
Domingos Sávio dos Santos ◽  
Michele Placedino Andrade ◽  
Mary Suzan Varaschin ◽  
Antônio Marcos Guimarães ◽  
Christian Hirsch
Keyword(s):  
Skeletal Muscle ◽  
Genetic Differentiation ◽  
Phylogenetic Tree ◽  
Neospora Caninum ◽  
Minas Gerais ◽  
Genetic Characteristics ◽  
Three Samples ◽  
Bovine Fetuses ◽  
Host Parasite ◽  
The Brain

Neosporosis is an important cause of abortion in cattle and information on their genetics and host parasite relationships are desirable. Neospora caninum samples obtained from 24 bovine fetuses from Minas Gerais, were genetically analyzed in part of the rDNA region, coding for rRNAs. Previously, brain, heart, liver, skeletal muscle, lung, kidney, spleen, thymus, lymph nodes, ovary or testis, uterus and skin of the ear were analyzed by conventional histopathology and immunohistochemistry. Of these, eight had lesions compatible in the brain and heart and two also in skeletal muscle and liver. Three brains showed antigen identification in immunohistochemistry. Brain and heart tissues were subjected to DNA extraction for PCR, whose product of 588 bp of ITS-1 region was sequenced in three samples. We obtained 96% similarity with dozens of sequences N. caninum deposited in GenBank. The phylogenetic tree showed great conservation among isolates of N. caninum in this study and those deposited in GenBank, while well-defined and specific branches were generated against other Apicomplexa. PCR for this region is useful as a diagnosis, with good analytical specificity, but the ITS-1 region is not suitable for genetic differentiation intra species because the sequences obtained were identical to the others analyzed.

scholarly journals Neospora caninum in properties in the west region of Paraná, Brazil: prevalence and risk factors

2018 ◽  
Vol 27 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Alessandra Snak ◽  
Felipe Gustavo Garcia ◽  
Arielle Aparecida Lara ◽  
Hilda Fátima Jesus Pena ◽  
Silvia Cristina Osaki
Keyword(s):  
Risk Factors ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Intermediate Hosts ◽  
Serum Samples ◽  
West Region ◽  
Bovine Fetuses ◽  
History Of ◽  
Polymerase Chain

Abstract Neospora caninum is a heteroxenous protozoa, whose definitive hosts are canids and intermediate hosts are herbivores, and is of great importance in cattle. The objectives of this study were to determine the prevalence of N. caninum in dairy cattle and dogs, to detect the presence of the protozoa at the molecular level in aborted fetuses, and to identify the risk factors associated with infection in properties in the western region of the state of Paraná. For this study, 600 bovine serum samples from 60 properties, 163 canine serum samples from 52 properties and 17 bovine fetuses from nine properties were collected. Data were collected using an epidemiological questionnaire to verify the risk factors. Serum samples were analyzed using the indirect fluorescent antibody test. Fetal tissues were analyzed using polymerase chain reaction and subsequent DNA sequencing. Of the bovine samples, 23.67% were positive for N. caninum. Among the canine samples, 11.66% were positive for N. caninum. Risk factors in cattle were history of abortion, low milk production, extensive breeding, and Jersey breed (p<0.05). Protozoan DNA was detected in 52.94% of the 17 fetuses and the sequencing presented high similarity with N. caninum.

scholarly journals Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR

2009 ◽  
Vol 18 (04) ◽  
pp. 14-19 ◽  
Author(s):  
A. D. Cabral ◽  
C. N. Camargo ◽  
N. T. C. Galleti ◽  
L. H. Okuda ◽  
E. M. Pituco ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Neospora Caninum ◽  
Bovine Fetuses

Vertical transmission of Neospora caninum in bovine fetuses from a slaughterhouse in Brazil

2019 ◽  
Vol 51 (6) ◽  
pp. 1751-1755 ◽  
Author(s):  
Lanna Vivien de Aquino Diniz ◽  
Ana Flávia Minutti ◽  
Beatriz de Souza Lima Nino ◽  
Letícia Ramos Costa ◽  
Maria Rachel Melo Bosculo ◽  
...  
Keyword(s):  
Vertical Transmission ◽  
Neospora Caninum ◽  
Bovine Fetuses

scholarly journals Current trends in bovine abortion in Argentina

2019 ◽  
Vol 39 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Eleonora L. Morrell ◽  
Carlos M. Campero ◽  
Germán J. Cantón ◽  
Anselmo C. Odeón ◽  
Dadín P. Moore ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Economic Losses ◽  
Dairy Herds ◽  
Diarrhea Virus ◽  
Current Trends ◽  
Infectious Origin ◽  
Bovine Fetuses ◽  
Bovine Abortion

ABSTRACT: Bovine abortion is an important cause of significant economic losses in beef and dairy herds. This syndrome is usually difficult to diagnose. The aim of this study was to characterize bovine abortion causes in Argentina by standard diagnosis procedures (histology, bacterial and viral isolation) and other diagnostic tests like direct fluorescent antibody test (DFAT), fetal serology, immunohistochemistry (IHC), and PCR, showing their specific advantages and limitations. Necropsies were performed in 150 aborted bovine fetuses submitted to the diagnostic laboratories of Instituto Nacional de Tecnología Agropecuaria (INTA) Balcarce, Argentina. Etiological diagnosis was confirmed in 78 fetuses (52% of the cases). Most causes of abortion were of infectious origin, being Neospora caninum (14.67%), Campylobacter fetus sp. (9.33%), Leptospira spp. (7.33%) and Brucella abortus (6.65%) the main microorganisms identified. Bovine viral diarrhea virus (BVDV) and bovine herpes virus (BHV) were diagnosed in 2 (1.33%) and 3 (2%) cases, respectively. This study showed a better characterization of bovine abortion compared with previous researches done on this topic.

scholarly journals The occurrence of anti-Neospora caninum in bovine female animals and bovine fetuses in Nossa Senhora do Livramento County, Mato Grosso

2016 ◽  
Vol 37 (6) ◽  
pp. 4161
Author(s):  
Robson Souza Rodrigues ◽  
Michelle Igarashi ◽  
Livia Saab Muraro ◽  
Ana Helena Benetti Gomes ◽  
Daniel Moura Aguiar ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Bos Indicus ◽  
Parasitic Disease ◽  
Immunofluorescence Technique ◽  
Mato Grosso ◽  
Blood Samples ◽  
Obligate Intracellular ◽  
Bovine Fetuses

Neosporosis is a parasitic disease caused by the obligate intracellular protozoan Neospora caninum, which is responsible for miscarriage cases in cattle countrywide. The vertical or transplacental route with prevalent miscarriages between the 3rd and 6rd months of pregnancyis the main transmission form in cattle. The aim of the present study is to investigate the presence of anti-N.caninum antibodies in (Bos indicus) Nellore female animals and fetuses in Nossa Senhora do Livramento County - MT. Blood samples from 489 female bovines and from 128 bovine fetuses over three months of pregnancy were colleted. The research about the anti N. caninum antibodies in bovine females and fetuses was conducted through the Immunofluorescence technique (IFA). We found 2.45% (12) seropositive and 3.91% (5) seropositive to N. caninum in the present study.

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