Rapid expression screening of Caenorhabditis elegans homeobox open reading frames using a two-step polymerase chain reaction promoter-gfp reporter construction technique

Gene ◽  
1998 ◽  
Vol 212 (1) ◽  
pp. 127-135 ◽  
Author(s):  
Giuseppe Cassata ◽  
Hiroshi Kagoshima ◽  
René F. Prétôt ◽  
Gudrun Aspöck ◽  
Gisela Niklaus ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Caenorhabditis Elegans ◽  
Open Reading Frames ◽  
Chain Reaction ◽  
Construction Technique ◽  
Expression Screening ◽  
Gfp Reporter ◽  
Polymerase Chain ◽  
Reading Frames ◽  
Step Polymerase Chain Reaction

scholarly journals Study of Legionella pneumophila Detection by Two Step Polymerase Chain Reaction

1993 ◽  
Vol 67 (11) ◽  
pp. 1062-1067
Author(s):  
Michio KOIDE ◽  
Atsushi SAITO ◽  
Futoshi HIGA ◽  
Yuuko YAMASHIRO ◽  
Tomohiko ISHIMINE ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Legionella Pneumophila ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Step Polymerase Chain Reaction

scholarly journals Site-selected insertion of the transposon Tc1 into a Caenorhabditis elegans myosin light chain gene.

1993 ◽  
Vol 13 (2) ◽  
pp. 902-910 ◽  
Author(s):  
A M Rushforth ◽  
B Saari ◽  
P Anderson
Keyword(s):  
Polymerase Chain Reaction ◽  
Caenorhabditis Elegans ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Null Alleles ◽  
Chain Gene ◽  
Splice Sites ◽  
Chain Reaction ◽  
Light Chain Gene ◽  
Polymerase Chain

We used the polymerase chain reaction to detect insertions of the transposon Tc1 into mlc-2, one of two Caenorhabditis elegans regulatory myosin light chain genes. Our goals were to develop a general method to identify mutations in any sequenced gene and to establish the phenotype of mlc-2 loss-of-function mutants. The sensitivity of the polymerase chain reaction allowed us to identify nematode populations containing rare Tc1 insertions into mcl-2. mlc-2::Tc1 mutants were subsequently isolated from these populations by a sib selection procedure. We isolated three mutants with Tc1 insertions within the mlc-2 third exon and a fourth strain with Tc1 inserted in nearby noncoding DNA. To demonstrate the generality of our procedure, we isolated two additional mutants with Tc1 insertions within hlh-1, the C. elegans MyoD homolog. All of these mutants are essentially wild type when homozygous. Despite the fact that certain of these mutants have Tc1 inserted within exons of the target gene, these mutations may not be true null alleles. All three of the mlc-2 mutants contain mlc-2 mRNA in which all or part of Tc1 is spliced from the pre-mRNA, leaving small in-frame insertions or deletions in the mature message. There is a remarkable plasticity in the sites used to splice Tc1 from these mlc-2 pre-mRNAs; certain splice sites used in the mutants are very different from typical eukaryotic splice sites.

Low frequency of CYP2A6 gene polymorphism as revealed by a one-step polymerase chain reaction method

1999 ◽  
Vol 9 (3) ◽  
pp. 327-332 ◽  
Author(s):  
Gen-Fu Chen ◽  
Yong-Ming Tang ◽  
Bridgett Green ◽  
Dong-Xin Lin ◽  
F. Peter Guengerich ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Polymorphism ◽  
Low Frequency ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
One Step ◽  
Polymerase Chain ◽  
Cyp2a6 Gene ◽  
Step Polymerase Chain Reaction
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