Cellular response in normal human cells exposed to chronically low-dose radiation in heavy-ion radiation field

2002 ◽  
Vol 1236 ◽  
pp. 323-326
Author(s):  
Masao Suzuki ◽  
Hiroshi Yasuda ◽  
Ryonfa Lee ◽  
Chisa Ohira ◽  
Hideyuki Majima ◽  
...  
Keyword(s):  
Radiation Field ◽  
Cellular Response ◽  
Low Dose ◽  
Heavy Ion ◽  
Human Cells ◽  
Low Dose Radiation ◽  
Normal Human ◽  
Heavy Ion Radiation ◽  
Normal Human Cells ◽  
Dose Radiation

scholarly journals Phosphoprotein profiles of candidate markers for early cellular responses to low-dose γ-radiation in normal human fibroblast cells

2017 ◽  
Vol 58 (3) ◽  
pp. 329-340 ◽  
Author(s):  
Ji-Hye Yim ◽  
Jung Mi Yun ◽  
Ji Young Kim ◽  
In Kyung Lee ◽  
Seon Young Nam ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Human Fibroblast ◽  
Low Dose ◽  
High Dose ◽  
Normal Human Fibroblast ◽  
Low Dose Radiation ◽  
Γ Radiation ◽  
Normal Human ◽  
Dose Radiation ◽  
In Cells

Abstract Ionizing radiation causes biological damage that leads to severe health effects. However, the effects and subsequent health implications caused by exposure to low-dose radiation are unclear. The objective of this study was to determine phosphoprotein profiles in normal human fibroblast cell lines in response to low-dose and high-dose γ-radiation. We examined the cellular response in MRC-5 cells 0.5 h after exposure to 0.05 or 2 Gy. Using 1318 antibodies by antibody array, we observed ≥1.3-fold increases in a number of identified phosphoproteins in cells subjected to low-dose (0.05 Gy) and high-dose (2 Gy) radiation, suggesting that both radiation levels stimulate distinct signaling pathways. Low-dose radiation induced nucleic acid–binding transcription factor activity, developmental processes, and multicellular organismal processes. By contrast, high-dose radiation stimulated apoptotic processes, cell adhesion and regulation, and cellular organization and biogenesis. We found that phospho-BTK (Tyr550) and phospho-Gab2 (Tyr643) protein levels at 0.5 h after treatment were higher in cells subjected to low-dose radiation than in cells treated with high-dose radiation. We also determined that the phosphorylation of BTK and Gab2 in response to ionizing radiation was regulated in a dose-dependent manner in MRC-5 and NHDF cells. Our study provides new insights into the biological responses to low-dose γ-radiation and identifies potential candidate markers for monitoring exposure to low-dose ionizing radiation.

scholarly journals Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

2019 ◽  
Author(s):  
Nadezhda S. Kudryasheva ◽  
Ekaterina S. Kovel
Keyword(s):  
Cellular Response ◽  
Low Dose ◽  
Fluorescent Proteins ◽  
Statistical Processing ◽  
Exposure Dose ◽  
Physiological Parameter ◽  
Low Dose Radiation ◽  
Low Intensity ◽  
Proper Number ◽  
Dose Radiation

Current paper reviews applications of luminescence bioassays for monitoring low-intensity factors, namely, radioactivity of different types (alpha, beta and gamma), and bioactive compounds (humic substances and fullerenols). Luminescence intensity is taken as a physiological parameter of luminous organisms. High rates of luminescence response can provide (1) a proper number of experiments under comparable conditions and, therefore, proper statistical processing, with this being highly important for ‘noisy’ low-dose exposures; (2) non-genetic, i.e. biochemical and physicochemical mechanisms of cellular response, in accordance to “exposome” concept. Bioassays based on luminous marine bacteria, their enzymes, and fluorescence coelenteramide-containing proteins were used to compare results of low-intensity exposures at cellular, biochemical and physicochemical levels, respectively. Results of the cellular exposures were discussed in terms of hormesis concept. Bioluminescence time dependence under low-dose radiation exposures corresponded to hormesis or threshold models; no bioluminescence monotonic dependency on intensity of exposure (dose rate, radioactivity, concentration) was found. Bioluminescence activation and absence of its dependency on intensity of exposure can be accepted as features of cellular adaptive response. Changes of biological luminescence were analyzed and discussed for bioassays of lower organization level – enzymes and florescent protein.

scholarly journals Radiation-quality Dependent Cellular Response in Mutation Induction in Normal Human Cells

2009 ◽  
Vol 50 (5) ◽  
pp. 395-399 ◽  
Author(s):  
Masao SUZUKI ◽  
Chizuru TSURUOKA ◽  
Yukio UCHIHORI ◽  
Hisashi KITAMURA ◽  
Cui Hua LIU
Keyword(s):  
Cellular Response ◽  
Human Cells ◽  
Mutation Induction ◽  
Radiation Quality ◽  
Normal Human ◽  
Normal Human Cells

scholarly journals The response of human mesenchymal stem cells to internal exposure to tritium β-rays

2019 ◽  
Vol 60 (4) ◽  
pp. 476-482 ◽  
Author(s):  
Yi Quan ◽  
Jinxian Lin ◽  
Bing Deng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cellular Response ◽  
Low Dose ◽  
Biological Effects ◽  
Tritiated Water ◽  
Human Mesenchymal Stem Cells ◽  
Internal Exposure ◽  
Low Dose Radiation ◽  
Dose Radiation

Abstract There is no doubt that estimating the exposure risk of external and internal low-dose radiation is an imperative issue in radiobiological study. Human mesenchymal stem cells (hMSCs) are multipotent and self-renewing, supporting the regeneration of damaged tissue, including tissue damaged by radiation. However, the responses of hMSCs to internal exposure to radionuclides are still insufficiently understood. In order to evaluate the adverse effects produced by internal exposure to tritiated water (HTO) at a low dose, hMSCs were exposed to 2 × 107 Bq/ml HTO, and the biological effects after the exposure were examined. Apoptosis and DNA double-strand breaks (DSBs) were assayed to analyze the cellular response to the damage induced by HTO. Slight enhancement of apoptosis was found after treatment, except at the dose of 9 mGy. The number of DSBs at 24 h post-irradiation showed that the DNA damage was able to be efficiently repaired by the hMSCs. Moreover, the increasing proportion of the cell population in S phase proved that the persistence of residual γH2AX foci at lower concentrations of HTO was attributable to the secondary production of DSBs in DNA replication. Our work adds to the available data, helping us understand the risk of stem cell transformation due to internal exposure and its correlation with low-dose radiation–induced carcinogenesis.

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