LC method for the determination of assay and purity of sibutramine hydrochloride and its enantiomers by chiral chromatography

Abstract A rapid HPLC method for the analytical resolution of cinacalcet enantiomers was developed. Four chiral columns (two amylose and two cellulose type) were evaluated in RP systems. Excellent enantioseparation with a resolution of more than 6 was achieved on Chiralpak AY (amylose 5-chloro-2-methylphenylcarbamate chiral stationary phase) using 10 mM triethylamine (pH 8.0)–acetonitrile (40 + 60, v/v) mobile phase. Validation of the HPLC method, including linearity, LOD, LOQ, precision, accuracy, and selectivity, was performed according to the International Conference on Harmonization guidelines. The method was successfully applied for the determination of (S)-cinacalcet in enantiopure active pharmaceutical ingredient (R)-cinacalcet.

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Reversed‐Phase HPLC Determination of Sibutramine Hydrochloride in the Presence of its Oxidatively‐Induced Degradation Products

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1081/jlc-120018897 ◽

2003 ◽

Vol 26 (6) ◽

pp. 977-986 ◽

Cited By ~ 7

Author(s):

A. I. Segall ◽

E. A. Collado ◽

R. A. Ricci ◽

M. T. Pizzorno

Keyword(s):

Degradation Products ◽

Reversed Phase ◽

Hplc Determination ◽

Reversed Phase Hplc ◽

Sibutramine Hydrochloride

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A method for determination of the plasma levels of modafinil enantiomers, (±)-modafinic acid and modafinil sulphone by direct human plasma injection and bidimensional achiral–chiral chromatography

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2007.03.004 ◽

2008 ◽

Vol 46 (5) ◽

pp. 937-944 ◽

Cited By ~ 16

Author(s):

Quezia B. Cass ◽

Túlio Ferreira Galatti

Keyword(s):

Human Plasma ◽

Plasma Levels ◽

Chiral Chromatography ◽

Plasma Injection

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Chromatographic methods for determination of pork biochemical indicators

Veterinariya, Zootekhniya i Biotekhnologiya ◽

10.36871/vet.zoo.bio.202008009 ◽

2020 ◽

Vol 1 (8) ◽

pp. 71-77

Author(s):

N. V. Bogolyubova ◽

◽

O. A. Voronina ◽

S. Yu. Zaytsev ◽

◽

...

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Nutrient Content ◽

Reversed Phase ◽

Chiral Chromatography ◽

Chromatographic Methods ◽

Green Chromatography ◽

Multidimensional Hplc ◽

Porous Sorbents

The study of the qualitative composition of the muscle tissue of animals is necessary in connection with the influence of the nutrient content on the technological, taste and dietary properties of meat. The major known changes in the biochemical parameters of the animal tissues are in the content of amino acids and fatty acids, etc. The aim of the work was to compare different chromatographic methods and to identify the most effective of them for the determination of fatty acids and amino acids in pig meat. The following HPLC methods are known for analyzing food products: ultra-HPLC, «hydrophilic» chromatography, two-dimensional and multidimensional HPLC methods, chiral chromatography, express chromatography, capillary HPLC, nano-HPLC, micellar HPLC, HPLC on monolithic columns, HPLC on surface porous sorbents, immunochromatographic methods, green chromatography, high-temperature HPLC. The most common methods for the determination of amino acids and fatty acids are reversed-phase and cation-exchange HPLC.

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