An Overview in Green Chromatographic Analysis from Pharmaceutical Preparations

Background: Green Chemistry has emerged as a new field of study in chemistry in the past 15 years and is intended to be sustainable at the molecular level. Green Chemistry has focused on harmless chemical technologies to meet both environmental and targets. The purpose of Green Chromatography is to protect the environment and the analyst like Green Chemisty. Objective: The main target is to reduce the consumption of hazardous solvent and to replacement of toxic and environmentally hazardous solvents with more benign alternatives. Methods: In addition, reducing the flow rate (decrease column interdiameter etc.) in order to miniaturizing the waste generation is also within the field of green chromatography. On the other hand, the most effective technique for the “Green” approach can be miniaturized completely. Flow rate decreases from mL to microliter per minute. Thus, waste production and solvent consumption are significantly reduced. In recent studies, studies without organic solvents such as SFC, SBWC are frequently encountered. Results: Chromatographic analysis has the potential to become greener at all stages from sample collection and preparation to separation and final determination. Liquid chromatography is often used in pharmaceutical analyzes. Tons of wastes are produced every year for pharmaceutical analysis. For this reason, greening approach in liquid chromatography becomes very important for human and environmental health in pharmaceutical analysis. Conclusion: This review discusses the approaches used to achieve the goals of green chromatography, aimed at protecting the environment and the analyst in pharmaceutical analysis. While one cannot completely convert the analysis to the green, the steps taken to green are very valuable particularly in widerange used HPLC both in drug quality control laboratories in industry and in reaserch studies.

Chromatographic methods for determination of pork biochemical indicators

The study of the qualitative composition of the muscle tissue of animals is necessary in connection with the influence of the nutrient content on the technological, taste and dietary properties of meat. The major known changes in the biochemical parameters of the animal tissues are in the content of amino acids and fatty acids, etc. The aim of the work was to compare different chromatographic methods and to identify the most effective of them for the determination of fatty acids and amino acids in pig meat. The following HPLC methods are known for analyzing food products: ultra-HPLC, «hydrophilic» chromatography, two-dimensional and multidimensional HPLC methods, chiral chromatography, express chromatography, capillary HPLC, nano-HPLC, micellar HPLC, HPLC on monolithic columns, HPLC on surface porous sorbents, immunochromatographic methods, green chromatography, high-temperature HPLC. The most common methods for the determination of amino acids and fatty acids are reversed-phase and cation-exchange HPLC.

Development and validation of green chromatography for the determination of anthocyanins in haskap berry, mulberry and blackberry

This study was aimed at developing and validating a green HPLC-DAD method to determine anthocyanins using ethanol and an alpha-hydroxy acid aqueous solution as a mobile phase.