Growth and survival of Aeromonas hydrophila in rice pudding (milk rice) during its storage at 4°C and 12°C

The growth and survival of Aeromonas hydrophila in three types of natural mineral waters were investigated. Mineral waters with different levels of mineral content (low, medium, and high) were experimentally contaminated with A. hydrophila, stored at different temperatures (10°C and 20°C), and analyzed at intervals over a 60-day period. Water samples that were not experimentally contaminated were investigated for indigenous A. hydrophila. The results confirmed that A. hydrophila may occur naturally in mineral waters and showed that the level of mineral content, temperature, length of storage, and, in some cases, the type of container used may favor the growth of A. hydrophila. The greatest proliferation was observed in water with a low mineral content stored in PET bottles at 10°C, in which A. hydrophila peaked at day 28 (4.47 ± 0.01 log CFU/100 ml). At 20°C, the same load was observed at day 60. The presence of high densities of A. hydrophila in bottled mineral water can constitute a risk for some groups of consumers, such as elderly and immunocompromised persons.

Download Full-text

Protection of Thiophenone in Catfish (Clarias sp.) Larvae When Challenged with Aeromonas hydrophila

Advances in Tropical Biodiversity and Environmental Sciences ◽

10.24843/atbes.2021.v05.i01.p04 ◽

2021 ◽

Vol 5 (1) ◽

pp. 23

Author(s):

Ana Indriyanti ◽

Pande Gde Sasmita Julyantoro ◽

Ni Putu Putri Wijayanti

Keyword(s):

Water Quality ◽

Survival Rate ◽

Quorum Sensing ◽

Aeromonas Hydrophila ◽

Oxygen Supply ◽

Culture Period ◽

Control Treatment ◽

Absolute Weight ◽

Growth And Survival ◽

Quorum Sensing Inhibitor

This research aimed to determine the ability of quorum sensing inhibitor compound thiophenone to protect catfish (Clarias sp.) larvae when challenged with the pathogenic Aeromonas hydrophila. This research was conducted at Fisheries Laboratory, Faculty of Marine Science and Fisheries, Udayana University, from December 2019 to January 2020. The experiment was setting up with four treatments and three replications for each treatment. 10 larvae of catfish were maintained in aquarium 15 cm × 15 cm × 20 cm filled with 1 liter of freshwater and used aeration for oxygen supply. The treatments were treatment A (control), treatment B (addition of A. hydrophila 106 cfu/ml), treatment C (addition of thiophenone 10 µM), and treatment D (addition of A. hydrophila 106 cfu/ml and thiophenone 10 µM). The results showed that the addition of thiophenone 10 µM increased the survival of catfish larvae up to 73% when challenged with A. hydrophila. That was significantly difference (P<0,05) compare to treatment B with survival rate of 43% within 3 days of culture period. Although it was not statistically different (P>0,05), the highest absolute weight and length were found in treatment A of 0,47 g and 0,50 cm, respectively, while the lowest absolute weight and length were found in treatment B about 0,23 g and 0,17 cm, respectively. The water quality such as temperature, pH and DO were still within the range that supported the growth and survival of catfish larvae during this study.

Download Full-text

GROWTH AND SURVIVAL OF AEROMONAS HYDROPHILA IN COOKING BUTTER AT DIFFERENT STORAGE TEMPERATURES

Assiut Veterinary Medical Journal ◽

10.21608/avmj.1994.184330 ◽

1994 ◽

Vol 30.2 (60) ◽

pp. 147-153

Keyword(s):

Aeromonas Hydrophila ◽

Growth And Survival ◽

Storage Temperatures

Download Full-text

EFISIENSI PAKAN, PERTUMBUHAN, KELANGSUNGAN HIDUP DAN RESPON IMUN IKAN PATIN (Pangasius sp.) YANG DIBERI PAKAN BERSINBIOTIK

Jurnal Akuakultur Rawa Indonesia ◽

10.36706/jari.v5i2.7139 ◽

2018 ◽

Vol 5 (2) ◽

pp. 140-154

Author(s):

Enromauli Pangaribuan ◽

Ade Dwi Sasanti ◽

Mohamad Amin

Keyword(s):

Immune Response ◽

Aeromonas Hydrophila ◽

Feed Efficiency ◽

Clinical Symptoms ◽

Challenge Test ◽

Growth And Survival ◽

Randomized Design ◽

Commercial Feed ◽

Completely Randomized Design

ABSTRACTSynbiotic is a combination of probiotics and prebiotics can improve the feed efficiency, growth, survival and BAL population of fish. The purpose of this research was to know the effect of synbiotic feed to feed efficiency, growth, survival and immune response of catfish. This research conducted on October to December 2016 in Laboratorium Dasar Perikanan, Departement of Budidaya Perairan, Faculty of Agriculture, Sriwijaya University. The method used in the research is Completely Randomized Design (CRD) with six treatments and three replications. Catfish with synbiotic feed that treatment with the addition of commercial feed with synbiotic P0 (Control), P1 (2.5 ml Prebiotics + Probiotics 6 ml /kg), P2 (5.0 ml Prebiotics + Probiotics 6 ml /kg), P3 (7.5 ml Prebiotics + Probiotics 6 ml /kg), P4 (10.5 ml Prebiotics + Probiotics 6 ml /kg) and P5 (12.5 ml Prebiotics + Probiotics 6 ml /kg) for 30 days. The observed parameters were feed efficiency, growth, survival, BAL populations in the gut and clinical symptoms after challenge test. The results showed that provision addition of synbiotic in feed give significant effect to feed efficiency, growth and survival of catfish. The treatment P2 with synbiotic feed (5.0 ml Prebiotics + 6 ml Probiotics /kg) gives the feed efficiency (39,27%), the highest growth of weight (4.38 g), the highest growth of length (1.74 cm), the survival (97.78%), LAB (Lactid Acid Bacteria) population (3.51 x 104 CFU.mL-1) and survival after test challenge with A. hydrophila (86.67%). Keywords: Sinbiotic, Pakan, Pangaius sp, Aeromonas hydrophila

Download Full-text

Survival and Growth Potential of Aeromonas hydrophila in Reconditioned Pork-Processing-Plant Water†

Journal of Food Protection ◽

10.4315/0362-028x-59.8.881 ◽

1996 ◽

Vol 59 (8) ◽

pp. 881-885 ◽

Cited By ~ 8

Author(s):

SAMUEL A. PALUMBO ◽

JEFFREY CALL ◽

BINH HUYNH ◽

JOSEPH FANELLI

Keyword(s):

Aeromonas Hydrophila ◽

Growth Potential ◽

Residual Chlorine ◽

Processing Plant ◽

Plant Water ◽

Survival Times ◽

Term Survival ◽

Growth And Survival ◽

Survival And Growth

The growth and survival of Aeromonas hydrophila K144 was studied in reconditioned pork-processing-plant water. Neutralization of residual chlorine by thiosulfate permitted growth and long-term survival of the bacterium at temperatures ranging from 5 to 28°C; growth was also observed at 37 and 42°C but survival times were shorter. The coliform growth response, a bioassay system to measure the amount of nutrients available for microbial growth, for the reconditioned water was 2.91 ± 0.61, which agreed with our observation that this water contained sufficient nutrients to support about 3 log units of growth of A. hydrophila. Our results indicate that residual chlorine levels are necessary to prevent the growth of any A. hydrophila which might contaminate reconditioned water.

Download Full-text