MATURASI IKAN LELE MUTIARA Clarias gariepinus BETINA DENGAN ESTRADIOL-17? MELALUI PAKAN

Some problems in catfish culture are the spawning circle, female catfish begin to mature gonad at 10 months, and after 1,5 months can spawn again. The purpose of this study was to evaluate the process of accelerating maturation female mutiara catfish with the addition Estradiol-17? by oral treatments. The study was design used 3 treatments : 0 ml/kg feed, 0,4 ml/kg feed, 0,6 ml/kg feed with three individual replications. The result showed that the addition of Estradiol-17? significantly affected on reproductive performance : Gonado Somatic Index (GSI) 102,3% and eggs diameter 16,4% in female mutiara catfish compared with 0 ml/kg feed. Key words : Clarias gariepinus, Eggs Diameter, Estradiol-17ß, Gonadosomatic Index (GSI), Reproductive Performance.

LAMA WAKTU PENCEGAHAN SERANGAN Aeromonas hydrophilla PADA IKAN GABUS (Channa striata) YANG DIBERI PAKAN MENGANDUNG JINTAN HITAM (Nigella sativa)

Black cumin (Nigela sativa) is immunostimulant that is able to improve the mechanism of cellular and humoral immunity response of fish. The aimed of this study was to determine the effect of the different injection times of Aeromonas hydrophilla after feeding with black cumin 3.5% for 14 days on snakehead on the prevalence, total leukocyte, hematocrit, survival rate and absolute growth of snakehead. All the test fish were fed with 3.5% black cumin for 14 days. Fish were fed three times a day at satiation. The treatment P1: injected A. hydrophilla at 10 days after feeding, P2: injected A. hydrophilla at 15 days after feeding and P3: injected with A. hydrophilla 20 days after feeding. The parameters observed were prevalence, total leukocyte, hematocrit, survival rate and absolute growth of snakehead. The results showed the treatment of the difference in injection time significantly effect on the prevalence but did not significantly effect on the survival rate and absolute growth of snakehead. Feeding containing 3.5% black cumin for 14 days can protect snakehead for 20 days after feeding. Prevalence of fish, total leukocyte, hematocrit, survival rate, length growth and weight growth fish in treatment P3, were 26.67%, 3.36 x 104sel.mm-3, 38%, 53%, 1.24 cm and 2.07 g, respectively. Keywords: A. hydrophilla, Black cumin, Snakehead.

PENGARUH JENIS BAKTERI PROBIOTIK TERHADAP PERTUMBUHAN IKAN LELE MUTIARA YANG DIPELIHARA MENGGUNAKAN SISTEM BIOFLOK

The main problems faced by fish farmers are waste, uneaten feed and feces. Which can reduce water quality and have a significant impact on fish growth and survival. This study aims to determine the effect of giving various types of probiotics on the growth rate and viability of Mutiara catfish raised using the biofloc system. We used Lactobacillus sp., Bacillus subtilis, and Nitrobacter as probiotic bacteria. This study used a completely randomized design experimental method with 4 treatments and 3 replications, namely K = probiotic less/control, P1= Lactobacillus probiotic, P2= Bacillus subtilis probiotic, P3= Nitrobacter probiotic. Catfish are reared for 30 days, feeding 3 times a day with FR 5% of body biomass. The parameters observed were absolute length growth, survival, Feed Conversion Ratio (FCR) and Specific Growth Rate (SGR) which were analyzed using ANOVA (P <0.05) and Duncan's test to see the differences between treatments. The results showed that the bacteria probiotic treatment had no significant effect on FCR and fish survival, but had a Lactobacillus sp significant effect on growth in absolute length (3.96 ± 0.06 cm) and the growth rate of daily specific weight (5,45±0,18 %/day). To increase the length and weight growth of catfish fry, probiotic bacteria of the type Lactobacillus sp. on cultivation media.Key words :biofloc, growth rate, lactobacillus, mutiara catfish, probiotics

KAJIAN PEMBERIAN PAKAN BERBAHAN BAKU LOKAL DENGAN KANDUNGAN PROTEIN YANG BERBEDA TERHADAP PERTUMBUHAN IKAN GURAME (Osphronemus goramy Lac.)

Gourami fish (Osphronemus goramy Lac.) is a freshwater fish commodity that has high economic value because its very popular with all people. But the feed commonly used by gourami fish cultivators is factory-made feed, which is a commercial feed that usually costs quite a lot of money due to the material for making commercial feed, some of which still import from abroad. Therefore it is necessary to find a solution to reduce the cost of cultivation production by replacing commercial feed with homemade feed made from local raw materials. The purpose of this study was to examine the use of local raw materials formulated to make feed suitable for the growth of gourami fish (Osphronemus goramy Lac.) And to analyze the production costs of goramy feed made from local raw materials. This study consisted of 4 treatments and 3 replications using the completely randomized design (CRD) method. The treatments consisted of treatment A (30% protein), treatment B (33% protein), treatment C (36% protein), and treatment D (commercial). The results of this study indicate that the treatment of feed A, B, and C produced the same growth as commercial feed, and treatment of feed A resulted in the lowest feed production costs compared to other treatments. Key word: feed, growth, local raw materials, Oshphronemus goramy

MATURASI LELE MUTIARA Clarias gariepinus BETINA MELALUI PENAMBAHAN HORMON OODEV DAN ESTRADIOL-17ß PADA PAKAN

The problem in Mutiara strain of North African catfish Clarias gariepinus culture is that the production of both larvae and seeds is still inhibited by the seasonal reproductive cycle where the catfish only spawn once during the spawning season. The purposes of this study were to evaluate the effect of administration of oodev and estradiol-17ß on the maturity process and to determine the best doses in accelerating the maturion of female Mutiara strain. This study used a Completely Randomized Design with 5 treatments and 3 individual replications. The treatments were combination between dose Oodev (PMSG and Anti-Dopamine) and dose Estradiol-17ß in feed (both doses in ml/kg feed):A(0+0), B(0.5+0), C(0+0.6), D(0.5+0,6), E(1+1). The results of this study showed that the administration of Oodev and Estradiol-17ß significant affect on gonadal maturity index about 82.21% compared to control and acclerating maturation of female mutiara strain with doses the best is the treatment E (oodev 1 ml/kg feed+estradiol-17ß 1 ml/kg feed).Key words: Clarias gariepinus, dose, estradiol-17ß, maturation, oodev

PEMANFAATAN EKSTRAK DAUN SEMBUNG (Blumea balsamifera L) UNTUK PENGOBATAN PENYAKIT MOTIL AEROMONAS SEPTICAEMIA PADA IKAN LELE

Nga champora is one of the plants than can be antibacterial agent to treat Motile Aeromonas Septicemia (MAS) disease. Nga champora contain more than a hundred active compounds, but most of what is done for research is about flavonoids, alkaloids, steroids, tannin, glicosides and atsiri, which have bioactivity effects both in vivo and in vitro. The purpose of this study is to determine the effectiveness of MAS disease treatment using nga champora. The experimental method was used in this research using with five treatments, thai is: A (500 ppm), B (600 ppm), C (700 ppm), and Kontrol treatment. The results showed that the higher has the higher resistance to A. hydrophillawith a value of 5.7 mm (500 ppm) to 8.3 mm (700 ppm), and a life speed of up to 60% (700 ppm). Then it can be concluded that sembung leaf extract is effectively used as a cure for MAS disease Keywords: alternatif treatment, bacterial disease, immune respon, maseration

POTENSI ANTIOKSIDAN EKSTRAK METANOL DIATOM Amphora sp.

Diatoms are a type of microalgae with a quite strong potential in the health sector as antioxidants, anticancer, and antivirals. But until now in Indonesia is still very rarely carried out research on the antioxidant potential of diatoms. The purpose of this study was to determine the antioxidant potential of Amphora sp. extracted with methanol. The method used in the extraction process is maceration, with a ratio of sample and methanol 1: 3 (300 g: 900 ml). The evaporation process compacted maserate resulting from maceration, then analyzed the total chlorophyll content, total flavonoids, and total phenols. Amphora sp. extraction results showed good potential with 7.08 ppm chlorophyll content, total flavonoids 6,299 ppm, and total phenol 7,085 ppm, and IC50 DPPH values of 3332.5 ppm. The IC50 value of the extract of Amphora sp. still classified as very weak antioxidant activity, but in general the extract of Amphora sp. has other bioactive potentials such as chlorophyll, flavonoids, and total phenol which can be used as material for further studies for the development of natural medicinal ingredients from Amphora sp.Key words: antioxidant, diatome, DPPH, macaration, methanol.

ANALISIS MUTU AIR AKIBAT AKTIVITAS PENAMBANG PASIR DENGAN METODE STORET DI SUNGAI SP PADANG OKI

Sand mining in the village of Sp Padang is carried out in an open pit mining model through several processes, for example the washing process which is carried out to separate sand from other components.When the sludge in the form of mud and fine sand enters the river, this part causes the quality of river water around the sand mining location to decline. The purpose of this research was to determine the condition of water quality due to sand mining in the river Sp.Padang.The type of data collected is primary data. The research was conducted at 3 stations on the river Sp.Padang.The physico-chemical parameters of river water are used, namely: turbidity, temperature, pH, TSS, DO, BOD, COD, water discharge and current velocity. The data obtained from the laboratory were then analyzed, then comparisons were made with the Storet method.Sampling for water quality replaces the surrounding area with direct measurements and measurements made in the laboratory.The use of the Storet method refers to PP No. 82 of 2001. The principle of Storet is to integrate river water quality data with river water standards and then adjust it according to its use, by classifying water quality into four classes. The results of the data analysis of the quality of river water in Sp.Padang as a result of the sand mining activities are categorized as good class B (lightly polluted) with a score of -6. Sand mining activity affects the water quality of the Sirah River in Padang Island, OKI Regency.Key words : OKI, sand miners, storet method, water quality

KEANEKARAGAMAN JENIS PAKAN PADA LAMBUNG IKAN SEPAT SIAM (Trichogaster pectoralis) DI RAWA BANJIRAN DESA SEDANG KECAMATAN SUAK TAPEH KABUPATEN BANYUASIN

The research on the diversity of feed types in the snakeskin gouramy hull was carried out in August to Desember in swamp flood, Sedang village, Suak Tapeh subdistrict, Banyuasin District. The method used is a field study with determination of stations carried out by purposive random sampling of 3 stations, namely the Nebong Kuning swamp station, the Poron swamp station and the Banan swamp station. This study aims to determine the diversity of feed types in the Sepat Siam fish hull in swamp flood, Sedang village, Suak Tapeh subdistrict, Banyuasin District. Based on the result of study found the type of feed in the sepat siam fish hull consisted of 2 groups, namely phytoplankton and zooplankton, which consisted of 5 classes namely Bacillariophyceae, Clorophyceae, Cyanophyceae, Crustaceae and Maxillopoda. Based on the diversity index of the type of snakeskin gouramy hull in Nebong Kuning Swamp station, the Poron swamp station and the Banan swamp station are included in the medium category which indicates that the habitat of the aquatic environment in which the snakeskin gouramy live namely swamp flood, Sedang village, Suak Tapeh subdistrict, Banyuasin District quite balanced and moderate ecological pressure. Keywords : Diversity, Feed, Flood Swamp, Hull, Snakeskin Gouramy

PENGGUNAAN BATANG PISANG KEPOK (Musa paradisiaca formmatypica) DENGAN DOSIS BERBEDA DALAM MENUMBUHKAN PAKAN ALAMI

This research was conducted for a month, in the laboratory of the Faculty of Marine and Fisheries of Nusa Cendana University. The study aims to determine the use of a banana kepok stem (Musa paradisiaca formmatypica) with different doses to see the type of natural feeds growing and the abundance of it. The banana kepok stem (Musa paradisiaca formmatypica) generally contains nitrogen, phosphorus and potassium. Nitrogen and phosphorus important to growing of natural feeds, while potassium important to increase the body durability of it. This study uses different dosages to see the abundance of natural feed. The dose used is 50 g.L-1, 75 g.L-1, 100 g. L-1. The results of the study showed the type of natural feed that grows are Daphnia sp and Infusoria and the highest amount of natural feed density in the treatment with dose of 75 g that is Infusoria as much as 356x104 cells.ml-1 and Daphnia sp as much as 168x104 cells.ml-1. The use of stem banana kepok (Musa paradisiaca formmatypica) can be used as feed for fish. Keywords: Banana kepok stem (Musa paradisiaca formmatypica), Dosages, Natural feed