Comparative Analysis The Effect of Iodine, Ozone, and Peracetic Acid as A Disinfectant of Cantang Grouper Egg

Problems in the fish hatchery sector often occur such as high egg mortality or low quality of fish eggs. Optimizing biosecurity is one of the efforts to improve the quality of fish eggs, for example by adding disinfectants such as iodine, ozone, and peracetic acid. The use of chemical disinfectants will produce good results if the right dose, time, and commodity are used. In this study. This research aims (1) to determine whether the use of chemicals including iodine, ozone, and peracetic acid had an effect as a disinfectant to increase the hatching rate of grouper fish, (2) to determine the level of effectiveness and efficiency of the use of chemicals as a disinfectant. This research is an experimental research type. The research subjects included in the study were cantang grouper fish eggs at PT. Pakarti Daksa Segara who used the exploratory sampling method. The conclusion of this study indicates that there is an influence on the growth rate of the use of iodine, ozone and, peracetic acid chemicals as disinfectants of cantang grouper eggs and there is a comparison of the effectiveness and efficiency of the use of the three chemicals which includes the percentage of the hatching rate, the cost of the disinfectant material and the time, which is needed in the use of the disinfectant. Iodine has the best level of effectiveness from the comparison of hatching eggs of cantang grouper and peracetic acid is the most efficient type of chemical.

Effect of Feeding on Juvenile Growth Rate of Wader Fish (Rashbora argyrotaenia)

Aquaculture is an activity to develop, obtain, reproduce and produce a fishery with the help of human resources. One of the cultivation practices is wader fish (Rashbora argyrotaenia) which is known for its very slow growth and relatively small size. This study aims to determine the growth rate of wader fish with different feeds in Blado Kulon Village, Tegalsiwalan, Probolinggo. The feed used was feed A in the form of fish pellets as a control, feed B consisting of a mixture of conch flour, soybean flour, bran, and tapioca flour, while feed C consisted of a mixture of conch flour, cassava leaf flour, bran and tapioca flour. The study was conducted for 42 days. The parameters measured in this study were temperature, DO, and pH, as well as observing the growth in absolute weight, specific growth rate (SGR), survival rate, and feed concentration ratio (FCR). Observations were analyzed using One Way Analysis of Variance (ANOVA) and further tests were carried out using the Duncan test. The results showed that different feeding did not have a significant effect on growth rate, absolute weight, feed conversion ratio, and survival of wader fish. Water quality parameters measured include temperature with an average of 28.42oC - 28.54 oC, while pH has an average of 7.42 - 7.50, and dissolved oxygen (DO) with an average of 5.54 - 6, 23 mg / L.

Development Strategy to Improve Class Classification In Nusantara Fishery Port Pengambengan, Bali Province

In the framework of improving governance, functional dimensions, and performance of the fishing port, the Nusantara Fishery Port in Jembrana Bali compiles a "strategic plan" to improve the class classification of the Nusantara Fishing Port Pengambengan to become a class A Ocean Fishing Port, a number of management facility criteria are required as the main prerequisites. The purpose of this study was to determine the condition of the facilities at Nusantara Fishing Port Pengambengan and to determine the strategy for developing a fishing port at Nusantara Fishing Port Pengambengan. This research method uses descriptive qualitative and quantitative methods. Data collection techniques through direct observation and interviews using a questionnaire regarding the condition of the facilities at Nusantara Fishing Port Pengambengan, then data analysis using a SWOT analysis approach. Nusantara Pengambengan Fishery Port is a class B port that functions as a support for the management and utilization of fish resources and the environment, starting from pre-production, production, processing and marketing of fishery products. In supporting fishery activities, Nusantara Fishing Port Pengambengan is currently supported by various facilities, namely basic facilities, functional facilities, and supporting facilities. The results showed that there were several facilities in poor conditions, namely basic pier facilities, anchoring ponds, complex roads, and drainage, from supporting facilities, namely public toilets and guest houses. Several facilities are not available in the Nusantara Fishing Port Pengambengan construction, namely ship docks, net repair wards/places, laboratories, WWTPs, watchtowers, and perimeter fences. The strategies obtained from the results of this study include: (1) Improving the quality of the catch to provide a positive image for potential investors. (2) Improve administrative services to provide fishermen comfort when carrying out fishing operations. (3) Support is needed, especially the role of the government related to management and development.

Abundance of Plankton in the Waters of Geger Beach, Badung Regency, Bali

Geger Beach is located in Peminge traditional village area, Sawangan, Nusa Dua, Bali which is a tourism area and there is seaweed cultivation managed by local people. Such human activity will affect the quality of the waters which will result in an increase in nutrients and organic matter which can further lead to changes in the structure of plankton. The purpose of this study is to find out the types of plankton and the abundance of plankton found in the waters of Geger Beach using the pour method. This research was conducted in March 2021. Sampling is conducted once a week at three observation points. The total phytoplankton species obtained as many as 32 species consisting of 6 classes, namely Bacillariophyceae (23 genera), Xanthophyceae (1 genus), Cyanophyceae (3 genera), Ulvophyceae (1 genus), Conjugatophyceae or Zygnematophyceae (2 genera), Coscinodiscophyceae (2 genera). The dominant type of phytoplankton found during the study was the Gyrosigma fasciola species in the Bacillariophyceae class. Meanwhile, the total zooplankton obtained by 11 species consisting of 5 classes, namely Maxillopoda (3 genera), Hexanauplia (3 genera), Magnoliopsida, Branchiopoda, and Copepoda (1 genus). The dominant type of zooplankton found during the study was the Cyclops sp. species in the Maxillopoda class. The average abundance of phytoplankton is 120 cells/l, while the average abundance of zooplankton is 20 cells/l. The average diversity value of phytoplankton is 2,7, while the diversity of zooplankton is 1,4. The average dominance index value of phytoplankton is 0,06, while the average zooplankton dominance index is 0,25.

Percentage of Mangrove Canopy Cover and Mollusks Abundance in Benoa Bay Mangrove Ecosystem

This research was conducted to determine the condition of the mangrove ecosystem based on its canopy cover and to determine the types of mollusks (Bivalves and Gastropods) associated with the Benoa Bay mangrove ecosystem. Hemispherical photography is a method for observing mangrove canopy cover and line transect method for mollusks. The composition of mangrove species found in the research location were five species, namely Rhizophora stylosa, Rhizophora mucronata, Rhizophora apiculata, Bruguiera gymnorrhiza, and Avicennia marina. The percentage of mangrove canopy cover in the Benoa Bay mangrove ecosystem is in a good category (average = 76.59%). There are eight types of mollusks found in the research location. There are two types of bivalves (Polymesoda bengalensis and Gafrarium pectinatum) and six types of gastropods (Nerita balteata, Nerita picea, Neritina turrita, Pila ampullacea, Cassidula aurisfelis, and Littoraria melanostoma). The relationship between the percentage of mangrove canopy cover and abundance of mollusks in the Benoa Bay mangrove ecosystem is very strong (r) of 0.920. The higher the percentage value of mangrove canopy cover, the higher the mollusks abundance.

Effects of Different Types of Feed on the Growth and Survival Rate of Betta splendens

Betta fish (Betta splendens) are freshwater ornamental fish that is very popular because it’s beautiful colors and unique behavior. This study aims to determine the growth measuring weight and length as well as the survival rate of betta fish seeds by providing different types of food that was conducted in door at Tulungagung, East Java, Indonesia. The study was experimentally designed with completely randomized design consisting of 3 treatments and 4 replications. The treatments A was fed with Tubifex sp., treatment B with artificial food (pellets), and treatment C was consisted of a combination of Tubifex sp., and artificial food. The test fish were 30 days old with a stocking density of 5/plastic bucket, where feeding was done ad-libitum. The study was conducted for 42 days. The parameters measured in this study were the growth in absolute weight and absolute length and its survival rate. Observations were analyzed using One Way Analysis of Variance (ANOVA) and further tests were carried out using the Duncan test. The results show that different feeding strategies had a significant effect on the growth of absolute weight and absolute length, and had no significant effect on the survival rate of betta fish. Water quality parameters measured include an average temperature was valued at 25.01oC-25.87oC, while the average pH was 8.42-8.47, and average dissolved oxygen (DO) was 4.13-4.17 mg/L.

Malassezia sp. Infection Prevalence in Dermatitis Dogs in Badung Area

Malasseziosis is a common fungal infection in dogs and it is secondary to the initial underlying dermatitis infection. These infections can worsen the prognosis of disease in dogs. The study was conducted on 26 dogs that were treated in several veterinary clinics in the Badung, Bali. This study aimed to determine the incidence rate of Malassezia sp. in dogs with dermatitis. Samples were collected using tape smear method from the skin lesion and then by microscopic examination. The results were tabulated and analyze descriptively. The results showed that 15 of the 26 samples of dogs tested positive were infected with Malassezia sp. (58%). Infection is more common in male (60%) and geriatric dogs (40%). Lesions were more common in the ear, limb, vaginal and inguinal region of infected dogs

Effect of Giving Different Doses of Vitamin E in Feed to the Level of Gonad Maturity of Tilapia (Oreochromis niloticus)

One of the nutrients that can be given to brood feed fish to improve reproductive performance and egg quality is vitamin E. Vitamin E has a very important role in increasing fish reproduction because vitamin E functions as an antioxidant that can maintain the presence of fatty acids and prevent fat oxidation in the fish cell membrane and can accelerate the secretion of reproductive hormones. This study aims to determine the effect of vitamin E in feed at different doses on the level of gonad maturity of tilapia. The research was conducted at the Fisheries Laboratory of the Faculty of Marine and Fisheries, Udayana University from July to August 2020. The samples of fish tested were tilapia (Oreochromis niloticus) obtained from the Teratai fishing pond, Sanur Bali. Vitamin E used was Ovagrow Vit E with a vitamin E content of 50%. The CRD method (completely randomized design) was used with four treatments and three replications. The doses of vitamin E that were mixed into the feed were 0, 100, 300, and 500 mg/kg of feed. The results showed that the administration of vitamin E mixed in the feed at different doses had a significant effect on the achievement of gonad maturity level, gonad somatic index (GSI), and Fecundity (P <0.05). Provision of vitamin E at a dose of 300 mg/kg of feed was the best dose for ripening tilapia gonads. The gonad maturity level developed up to maturity stage IV, the GSI value was (3.56 ± 0.46) and the fecundity value was (1379 ± 289).

Profile of Leachate and Sea Water in The Waste Processing Area Suwung, Denpasar, Bali

This study aims to determine the profile of leachate, and seawater in the Suwung waste processing area (TPA Suwung). The method used in this research was descriptive quantitative. The results showed a high profile of untreated leachate with a TSS value of 9.407 mg/L, pH with a value of 8.17, DO with a value of 0.063 mg/L, BOD with a value of 375.5 mg/L, and COD with a value of 5.776 mg/L. The treated leachate with a TSS value of 4,987 mg/L, pH with a value of 8.52, DO with a value of 0.063 mg/L, BOD with a value of 321.9 mg/L, and a COD value with a value of 3.069 mg/L. Meanwhile, the seawater profile was classified as good with parameter values of TSS with a value of 76 mg/L, DO 7.54 mg/L, pH 7.54, BOD with a value of 13.6 mg/L, COD with a value of 64 mg/L. The existence of TPA Suwung has a negative impact and influence on seawater in the area. Revitalization efforts are needed to carry out leachate water treatment so that the effluent quality can be improved for the quality of water around TPA Suwung.

Effect of Thiophenone Compound on Motility and Caseinase Production of Aeromonas hydrophila

Motility and caseinase production are part of bacterial virulence factors which are regulated by a bacterial intercellular communication system, often called quorum sensing (QS). This study aims to determine the effect of the QS inhibitor compound thiophenone in reducing those virulence factors. This research was conducted at the Laboratory of Fisheries Science, Faculty of Marine Science and Fisheries, Udayana University, from December 2019 to March 2020. This study tested 3 different treatments with 3 repetitions for each treatment. Treatment A as control (without the addition of thiophenone), treatment B (addition of 5 ?M thiophenone), and C treatment (addition of 10 ?M thiophenone). The results showed that thiophenone compounds can inhibit the QS system of Aeromonas hydrophila. It can be seen from the significantly reduced (P<0.05) motility and caseinase enzyme activity of A. hydrophila compared to without addition of thiophenone compounds. The average diameter of the caseinase enzyme production produced in treatment A at 22 hours was 21.40±0.36 mm, in treatment B was 19.70±0.2 mm and in treatment, C was 17.87±0.05 mm. Whereas in motility, the resulting average diameter in treatment A at 22 hours was 6.57±0.61 mm, in treatment B was 5.67±0.35 mm and in treatment, C was 5.10±0.6 mm. These results indicate that the QS thiophenone inhibitor compound can reduce virulence factors, namely motility and caseinase production from pathogenic bacteria A. hydrophila. Treatment C can decrease virulence factors compared to treatment A and B.