Protection of Thiophenone in Catfish (Clarias sp.) Larvae When Challenged with Aeromonas hydrophila

Author(s):  
Ana Indriyanti ◽  
Pande Gde Sasmita Julyantoro ◽  
Ni Putu Putri Wijayanti

This research aimed to determine the ability of quorum sensing inhibitor compound thiophenone to protect catfish (Clarias sp.) larvae when challenged with the pathogenic Aeromonas hydrophila. This research was conducted at Fisheries Laboratory, Faculty of Marine Science and Fisheries, Udayana University, from December 2019 to January 2020. The experiment was setting up with four treatments and three replications for each treatment. 10 larvae of catfish were maintained in aquarium 15 cm × 15 cm × 20 cm filled with 1 liter of freshwater and used aeration for oxygen supply. The treatments were treatment A (control), treatment B (addition of A. hydrophila 106 cfu/ml), treatment C (addition of thiophenone 10 µM), and treatment D (addition of A. hydrophila 106 cfu/ml and thiophenone 10 µM). The results showed that the addition of thiophenone 10 µM increased the survival of catfish larvae up to 73% when challenged with A. hydrophila. That was significantly difference (P<0,05) compare to treatment B with survival rate of 43% within 3 days of culture period. Although it was not statistically different (P>0,05), the highest absolute weight and length were found in treatment A of 0,47 g and 0,50 cm, respectively, while the lowest absolute weight and length were found in treatment B about 0,23 g and 0,17 cm, respectively. The water quality such as temperature, pH and DO were still within the range that supported the growth and survival of catfish larvae during this study.

2021 ◽  
Vol 1 (1) ◽  
pp. 40-50
Author(s):  
Johanna L. Thenu ◽  
Lexon H. J. Tinglioy

Tilapia (Oreochromis niloticus) is one of the most popular fish species and has high economic value. Increased demand for this type of fish must be matched by cultivation efforts. Limited land for cultivation ponds, so it is necessary to look for alternatives, one of which is cultivation in seawater floating marine cages. This study aims to determine the manipulation process of freshwater tilapia into seawater, the rate of growth and survival, and analysis of its chemical composition. The research used was an experimental method. The results of measuring water quality including salinity increased by 3 ppt per day, temperatures ranged from 27-30 OC, DO was between 7.0-7.5 mg/L, pH between 7,2-7,6. The absolute weight and length were 408.5 g and 26 cm, respectively, the growth rate was 2.27%, and the survival rate was 85-90%. The chemical composition values ​​include moisture content of 71.59%, ash 1.54%, fat 4.74%, 21.3% protein, and 0.83% carbohydrates. The results showed that freshwater tilapia can be manipulated to live in seawater and have a good growth and survival rate and have a high chemical composition, especially protein and fat.


Author(s):  
Rahmad Afdillah ◽  
Yuli Andriani ◽  
Zahidah Hasan ◽  
. Rosidah ◽  
. Iskandar

This research aims was to determine the effective pressure on fine bubbles technology on the growth of Siamese catfish fry in the aquaponic system. This study uses a completely randomized design with four treatments and three replications. Treatment A (Control), Treatment B (FBs at a pressure of 4,5 atm), Treatment C (FBs at a pressure of 5 atm and treatment D (FBs at a pressure of 5,5 atm). The parameters observed were Specific Growth Rate, Survival Rate and water quality (Temperature, pH, DO, Ammonia) Data were analyzed using variance with the F test at a 95% confidence level. The best treatment for catfish growth was treatment D (5,5 atm pressure), which gave the highest SGR value of 7,24% and the highest SR value of 100%. The value of water quality parameters were in good condition for the growth and survival of catfish.


2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Muhammad Junaidi ◽  
Mat Sardi Hamzah

ABSTRACT The development of lobster farming in floating net cage in Ekas Bay caused an environmental degradation such as decrease water quality due to some aquaculture wastes. The purposes of this study were to determine the status of water quality and their effect on growth and survival rate of lobster reared in floating net cages (FNC) in the Ekas Bay, West Nusa Tenggara Province. Water sample collection and handling referred to the APHA (1992). Analyses of water quality data were conducted using Principal Component Analysis. Determination of the water quality status of Ekas Bay was performed with STORET system. Multivariate analyses were used to determine the relationship between water quality, growth, and survival rate of lobster reared in FNC. Results showed that Ekas Bay water quality status was categorized in class C (medium contaminated), which exceeded some quality standard parameters such as ammonia (0.3 mg/l), nitrate (0.008 mg/l), and phosphate (0.015 mg/l). During lobster farming activities feeding with trash fish for 270 days, we obtained daily growth rate of  0.74% (lower than normal growth rate of 0.86%), survival rate of 66% (lower than normal survival rate of 86.7%), and feed conversion ratio of 11.15. Ammonia was found as a dominant factor reducing growth  and survival rate of lobster reared in FNC. Keywords: water quality, lobsters, growth, survival, Ekas Bay


2018 ◽  
Vol 13 (2) ◽  
pp. 63
Author(s):  
Haryati Haryati ◽  
Yushinta Fujaya ◽  
Early Septiningsih

Live foods such as rotifers and Artemia are commonly used as foods in larval rearing of mud crab (S. olivacea). However, the continuous availability and nutritional consistency of live foods are difficult to control. Thus, the development of artificial diets to partially or fully replaced live foods is needed to overcome the limitations of live foods. The purpose of this research was to determine the best stage at which mud crab larvae can be weaned from live foods to artificial diets. The research experiment consisted of: treatment-1, the larvae were fed with live foods from zoea-1 to megalopa stages as the control treatment; treatment-2, the larvae were fed with artificial diet from zoea-2 to megalopa stages; and treatment-3, the larvae were fed with artificial diet from zoea-3 to megalopa stages. In treatment-4, artificial diet was given from zoea-4 to megalopa stages. The growth and survival rate of larvae in treatment-1, 3, and 4 were not significantly different (P>0.05) but significantly different with treatment-2. Based on the present results, this study suggests that artificial diet can be given to mud crab larvae (S. olivacea) from the third zoea stage.


2021 ◽  
Vol 273 ◽  
pp. 03008
Author(s):  
Evgeny Tikhonov ◽  
Valentin Bazykin ◽  
Igor Grigorev ◽  
Sergey Solovyov ◽  
Elena Radkevich

The aim of the study is to determine the effect of the feed type (Daphnia pulex-based feed and Dajana nano gran brand) on the water quality (presence of microorganisms and suspended solids) and on the growth and survival of Macrobrachium rosenbergii shrimp larvae. The study implementation plan involved 4 groups of shrimps placed under the same conditions, which were evaluated for 14 weeks through periodic observations and biometrics. The data obtained recorded significant differences between the groups where the best growth and survival results were obtained in organisms that received Dajana nano gran feed, regardless of water quality. In addition, the values of protein in shrimp meat up to 45% and different amounts of fatty acids were obtained. It was determined that the Daphnia pulex-based feed showed lower results in the growth rate and survival rate of Macrobrachium rosenbergii shrimp.


Author(s):  
Abdul Rakhfid ◽  
Udin Mauga

Vannamei shrimp (Litopenaeus vannamei) is a commodity that is expected to not only increase options for farmers but also sustain the rise of shrimp farming business in Indonesia. One important factor in shrimp farming is the availability of feed. In addition to the availability of natural feed during cultivation, stocking density is also very influential in the survival rate and growth of shrimp vannamei. The research was conducted in September-December 2016 farms Bonea Village District of Lasalepa Muna with Test the different dosages with different stocking density. The study used a Random Group Factorial, which is based fertilization (Factor A) with three levels and stocking density (Factor B) as many as three levels, each with three replications so that all 27 units experimental unit. The results showed that the combination of factors dose of 0.7 g of urea + 0.9 g TSP and stocking density factor of 10 individuals per container provides prawn post larvae growth vannamei most excellent and a combination of factors Urea fertilizer dosage of 0.9 g TSP + 1.1 g and stocking density factor of 20 individuals per container provides a survival rate of post larvae vannamei most good. Water quality media for research in the range that is optimal for the growth and survival of post larvae vannamei.


2013 ◽  
Vol 5 (2) ◽  
pp. 363-370 ◽  
Author(s):  
M. I. Hossain ◽  
M. I. Hossain ◽  
M. M. Kamal ◽  
M. A. Mannan ◽  
M. A. B. Bhuyain

The study was carried out for 138 days to know the growth and survival rate of P. monodon by applying probiotics in Gazi Fish Culture Ltd. Dacope, Khulna, Bangladesh. Six experimental ponds (4000 m2 in size i.e. one acre) were selected of which three were probiotic ponds and three were controlled. After pond preparation, PL15 (average weight of each 4.75±0.09 g) was stocked at the rate of 13 per m2 following polymerase chain reaction (PCR) test. CP NASA shrimp feed was used during the study period.  Transparency, salinity, pH, dissolved oxygen (DO), temperature, total Ammonia Nitrogen (TAN) were recorded by standard measurements. The average final body weight of the harvested shrimp is 37.67±1.15 g in probiotics ponds and 27.33±0.58 g in controlled ponds and the difference was significant (P < 0.01) between these two productions. The average survival rate was 90.67±1.15 % in probiotic pond and 71.00±3.0 % in controlled pond. The average daily gain (ADG) in weight was 0.27±0.01 g and 0.19±0.01 g in probiotic and controlled ponds, respectively. The result showed that probiotic plays an important role in maintaining water quality parameters, soil quality and health management as well as increases the growth and survival of shrimp.Key words: Probiotics; P. monodon; Water quality parameter; Growth rate; Survival rate.© 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v5i2.11815         J. Sci. Res. 5 (2), 363-370 (2013)


2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Muhammad Junaidi ◽  
Mat Sardi Hamzah

<p><strong><em>ABSTRACT</em></strong></p> <p><em>The development of lobster farming in floating net cage in Ekas Bay caused an environmental degradation such as decrease water quality due to some aquaculture wastes. The purposes of this study were to determine the status of water quality and their effect on growth and survival rate of lobster reared in floating net cages (FNC) in the Ekas Bay, West Nusa Tenggara Province. Water sample collection and handling referred to the APHA (1992). Analyses of water quality data were conducted using Principal Component Analysis. Determination of the water quality status of Ekas Bay was performed with STORET system. Multivariate analyses were used to determine the relationship between water quality, growth, and survival rate of lobster reared in FNC. Results showed that Ekas Bay water quality status was categorized in class C (medium contaminated), which exceeded some quality standard parameters such as ammonia (0.3 mg/l), nitrate (0.008 mg/l), and phosphate (0.015 mg/l). During lobster farming activities feeding with trash fish for 270 days, we obtained daily growth rate of  0.74% (lower than normal growth rate of 0.86%), survival rate of 66% (lower than normal survival rate of 86.7%), and feed conversion ratio of 11.15. Ammonia was found as a dominant factor reducing growth  and survival rate of lobster reared in FNC.</em></p> <p><em> </em></p> <strong><em>Keywords:</em></strong><em> water quality, lobsters, growth, survival, Ekas Bay</em>


Author(s):  
Hikmah Nurazizah ◽  
I Wayan Arthana ◽  
Gde Raka Anggara Kartika

Stocking density is an important factor in cultivation, it can save land use, and increase the efficiency of fish farming. The purpose of this research was to determine the different density information on tilapia seeds measuring 2-3 cm as an alternative to using tilapia (O. niloticus) seeds 7-9 cm in size so that they can be developed in Batur Lake. The research was conducted in January - March 2020 in the floating net cages in Trunyan Kintamani village, Bangli, Bali. The research used a completely randomized design method with three treatments and three repetitions. The Tilapia seeds used are 2-3 cm with different stocking densities, 100 ind/m3, 150 ind/m3, and 200 ind/m3. The parameters observed were SGR (Specific Growth Rate), absolute growth, SR (Survival Rate), FCR (Feed Conversion Ratio), and water quality including temperature, DO, pH, TDS. The results of this study indicated that stocking density did not have a significant effect on the growth and survival rate of tilapia seeds, because the temperature was too cold so that the tilapia seeds failed to adapt properly at the beginning of stocking and the physiological conditions of the fish were disturbed so that the fish experienced stress and went to died. Water quality parameters measured include temperature with a range value of 25.86-25.880C, pH with a range value of 9.08-9.13, DO (Dissolved oxygen) with a range value of 7.9-8 mg/L, and TDS (Dissolved solids) with a range value of 1040.25-1041.58 mg/L.


2015 ◽  
Vol 2 (2) ◽  
pp. 90
Author(s):  
Magfirah Magfirah ◽  
Saiful Adhar ◽  
Riri Ezraneti

Penelitian ini bertujuan untuk mengetahui bagaimana pengaruh surfaktan terhadap pertumbuhan, kelangsungan hidup dan histologi insang benih ikan nila. Penelitian dilaksanakan pada bulan Oktober hingga November 2014 diLaboratorium Hatchery dan Teknologi Program Studi Budidaya Perairan Fakultas Pertanian Universitas Malikussaleh Aceh Utara. Ikan diberi perlakuan dengan konsentrasi deterjen yang berbeda, perlakuan yang diberikan yaitu: perlakuan A (Kontrol), B (deterjen 3 %), C (Konsentrasi deterjen 6 %) dan D (Konsentrasi deterjen 9 %). Pengambilan data dilakukan setiap 7 hari sekali. Adapun rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan 3 ulangan dan dilanjutkan dengan Uji Beda Nyata Jujur jika terdapat perbedaan. Parameter yang diamati adalah parameter pada laju pertumbuhan, kelangsungan hidup, histologi insang dan efisiensi pakan serta parameter kualitas air (suhu dan pH). Hasil penelitian menunjukkan bahwa ikan nila yang diberi perlakuan konsentrasi deterjen 3 %, 6 %, 9 % berpengaruh terhadap pertumbuhan dan kelangsungan hidup. Perlakuan kontrol menghasilkan nilai, laju pertumbuhan dan efisiensi paling baik, yaitu masing-masing sebesar 2,84 gram, 97,36 %, sedangkan untuk kelangsungan hidup menunjukkan hasil yang terbaik pada perlakuan konsentrasi deterjen 3 % yaitu 100 %. Parameter kualitas air selama penelitian yang diukur antara lain adalah suhu air dengan kisaran 26,6-28,1 ᵒC, dan pH 7,1-7,8. This study aimed to know the effect of surfactant on growth, survival rate and gill histology of tilapia fingerling. It carried out on October to November 2014 at Hatchery and Aquaculture Technology Laboratory, Aquaculture Department Agriculture Faculty Malikussaleh University North Aceh. Experimented fish was given different concentrations of detergent. The treatments were A: control, B (detergent 3%), C (detergent 6%), and D (detergent 9%). Sampling data was done every seven days. Experimental design used was Completely Randomized Design with four treatments and three replications then it was continued by BNT test. Observed parameters were growth rate, survival rate, gill histology, feed efficiency, and water quality (temperature and pH). The result showed that different concentrations of detergent (3%, 6%, 9%) affected on growth and survival rate of tilapia fish. Control gave the best growth rate and feed efficiency which were 2,84 grams and 97,36%. While the highest survival rate was obtained in treatment of detergent 3% which was 100%. The water quality parameters during experiment were temperature ranged 26,6-28,1 ᵒC and pH ranged 7,1-7,8.


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