Expression of strictosidine β-D-glucosidase cDNA from Catharanthus roseus, involved in the monoterpene indole alkaloid pathway, in a transgenic suspension culture of Nicotiana tabacum

2001 ◽  
Vol 39 (9) ◽  
pp. 763-769 ◽  
Author(s):  
Rafael Zárate ◽  
Marc Bonavia ◽  
Arjan Geerlings ◽  
Robert van der Heijden ◽  
Robert Verpoorte
Keyword(s):  
Nicotiana Tabacum ◽  
Suspension Culture ◽  
Catharanthus Roseus ◽  
Indole Alkaloid

scholarly journals Development of a Cell Suspension Culture System for Promoting Alkaloid and Vinca Alkaloid Biosynthesis Using Endophytic Fungi Isolated from Local Catharanthus roseus

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 672
Author(s):  
Tran My Linh ◽  
Nguyen Chi Mai ◽  
Pham Thi Hoe ◽  
Ninh Thi Ngoc ◽  
Phan Thi Hong Thao ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Endophytic Fungi ◽  
Catharanthus Roseus ◽  
Cell Suspension Culture ◽  
Indole Alkaloids ◽  
Vinca Alkaloid ◽  
Indole Alkaloid ◽  
Cell Suspension Cultures ◽  
Cell Extracts

Cell and tissue cultures of Catharanthus roseus have been studied extensively as an alternative strategy to improve the production of valuable secondary metabolites. The purpose of this study was to produce C. roseus callus and suspension cell biomass of good quality and quantity to improve the total alkaloids and bis-indole alkaloids. The young stem derived-callus of C. roseus variety Quang Ninh (QN) was grown on MS medium supplemented with 1.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) plus 1.5 mg/L kinetin, and the growth rate increased by 67-fold after 20 days. The optimal conditions for maintaining the cell suspension culture were 150 mg/50 mL cell inoculum, a medium pH of 5.5 and a culture temperature of 25 °C. The low alkaloid content in the culture was compensated for by using endophytic fungi isolated from local C. roseus. Cell extracts of endophytic fungi—identified as Fusarium solani RN1 and Chaetomium funicola RN3—were found to significantly promote alkaloid accumulation. This elicitation also stimulated the accumulation of a tested bis-indole alkaloid, vinblastine. The findings are important for investigating the effects of fungal elicitors on the biosynthesis of vinblastine and vincristine, as well as other terpenoid indole alkaloids (TIAs), in C. roseus QN cell suspension cultures.

Differential expression of indole alkaloid pathway genes across Catharanthus roseus plant organs

2020 ◽  
Vol 52 (4) ◽  
Author(s):  
Ahmed M. Ramadan ◽  
Muna A. Abdulgader ◽  
Thana Khan ◽  
Nour O. Gadalla ◽  
Ahmed Bahieldin
Keyword(s):  
Differential Expression ◽  
Catharanthus Roseus ◽  
Indole Alkaloid ◽  
Plant Organs

scholarly journals Effect of Aspergillus flavus Fungal Elicitor on the Production of Terpenoid Indole Alkaloids in Catharanthus roseus Cambial Meristematic Cells

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3276
Author(s):  
Chuxin Liang ◽  
Chang Chen ◽  
Pengfei Zhou ◽  
Lv Xu ◽  
Jianhua Zhu ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Aspergillus Flavus ◽  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
Fungal Elicitor ◽  
Control Group ◽  
Terpenoid Indole Alkaloids ◽  
Meristematic Cells ◽  
Qrt Pcr ◽  
Induction Process

This study reported the inducing effect of Aspergillus flavus fungal elicitor on biosynthesis of terpenoid indole alkaloids (TIAs) in Catharanthus roseus cambial meristematic cells (CMCs) and its inducing mechanism. According to the results determined by HPLC and HPLC-MS/MS, the optimal condition of the A. flavus elicitor was as follows: after suspension culture of C. roseus CMCs for 6 day, 25 mg/L A. flavus mycelium elicitor were added, and the CMC suspensions were further cultured for another 48 h. In this condition, the contents of vindoline, catharanthine, and ajmaline were 1.45-, 3.29-, and 2.14-times as high as those of the control group, respectively. Transcriptome analysis showed that D4H, G10H, GES, IRS, LAMT, SGD, STR, TDC, and ORCA3 were involved in the regulation of this induction process. The results of qRT-PCR indicated that the increasing accumulations of vindoline, catharanthine, and ajmaline in C. roseus CMCs were correlated with the increasing expression of the above genes. Therefore, A. flavus fungal elicitor could enhance the TIA production of C. roseus CMCs, which might be used as an alternative biotechnological resource for obtaining bioactive alkaloids.

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