In Situ Burning of Spilled Oil in Freshwater Inland Regions of the United States

2003 ◽  
Vol 8 (4) ◽  
pp. 331-335 ◽  
Author(s):  
David E Fritz
Keyword(s):  
United States ◽  
The United States ◽  
Spilled Oil

scholarly journals International Arctic Systems for Observing the Atmosphere: An International Polar Year Legacy Consortium

2016 ◽  
Vol 97 (6) ◽  
pp. 1033-1056 ◽  
Author(s):  
Taneil Uttal ◽  
Sandra Starkweather ◽  
James R. Drummond ◽  
Timo Vihma ◽  
Alexander P. Makshtas ◽  
...  
Keyword(s):  
United States ◽  
The United States ◽  
Satellite Observations ◽  
The Arctic ◽  
International Polar Year ◽  
Network Measurements ◽  
International Polar ◽  
In Situ Observations ◽  
Arctic Atmosphere

Abstract International Arctic Systems for Observing the Atmosphere (IASOA) activities and partnerships were initiated as a part of the 2007–09 International Polar Year (IPY) and are expected to continue for many decades as a legacy program. The IASOA focus is on coordinating intensive measurements of the Arctic atmosphere collected in the United States, Canada, Russia, Norway, Finland, and Greenland to create synthesis science that leads to an understanding of why and not just how the Arctic atmosphere is evolving. The IASOA premise is that there are limitations with Arctic modeling and satellite observations that can only be addressed with boots-on-the-ground, in situ observations and that the potential of combining individual station and network measurements into an integrated observing system is tremendous. The IASOA vision is that by further integrating with other network observing programs focusing on hydrology, glaciology, oceanography, terrestrial, and biological systems it will be possible to understand the mechanisms of the entire Arctic system, perhaps well enough for humans to mitigate undesirable variations and adapt to inevitable change.

scholarly journals Genomics, Exometabolomics, and Metabolic Probing Reveal Conserved Proteolytic Metabolism of Thermoflexus hugenholtzii and Three Candidate Species From China and Japan

2021 ◽  
Vol 12 ◽  
Author(s):  
Scott C. Thomas ◽  
Devon Payne ◽  
Kevin O. Tamadonfar ◽  
Cale O. Seymour ◽  
Jian-Yu Jiao ◽  
...  
Keyword(s):  
United States ◽  
Amino Acids ◽  
Draft Genome ◽  
The United States ◽  
Pentose Phosphate ◽  
High Abundance ◽  
Comparative Genomic ◽  
Labeled Compounds ◽  
Geothermal Systems

Thermoflexus hugenholtzii JAD2T, the only cultured representative of the Chloroflexota order Thermoflexales, is abundant in Great Boiling Spring (GBS), NV, United States, and close relatives inhabit geothermal systems globally. However, no defined medium exists for T. hugenholtzii JAD2T and no single carbon source is known to support its growth, leaving key knowledge gaps in its metabolism and nutritional needs. Here, we report comparative genomic analysis of the draft genome of T. hugenholtzii JAD2T and eight closely related metagenome-assembled genomes (MAGs) from geothermal sites in China, Japan, and the United States, representing “Candidatus Thermoflexus japonica,” “Candidatus Thermoflexus tengchongensis,” and “Candidatus Thermoflexus sinensis.” Genomics was integrated with targeted exometabolomics and 13C metabolic probing of T. hugenholtzii. The Thermoflexus genomes each code for complete central carbon metabolic pathways and an unusually high abundance and diversity of peptidases, particularly Metallo- and Serine peptidase families, along with ABC transporters for peptides and some amino acids. The T. hugenholtzii JAD2T exometabolome provided evidence of extracellular proteolytic activity based on the accumulation of free amino acids. However, several neutral and polar amino acids appear not to be utilized, based on their accumulation in the medium and the lack of annotated transporters. Adenine and adenosine were scavenged, and thymine and nicotinic acid were released, suggesting interdependency with other organisms in situ. Metabolic probing of T. hugenholtzii JAD2T using 13C-labeled compounds provided evidence of oxidation of glucose, pyruvate, cysteine, and citrate, and functioning glycolytic, tricarboxylic acid (TCA), and oxidative pentose-phosphate pathways (PPPs). However, differential use of position-specific 13C-labeled compounds showed that glycolysis and the TCA cycle were uncoupled. Thus, despite the high abundance of Thermoflexus in sediments of some geothermal systems, they appear to be highly focused on chemoorganotrophy, particularly protein degradation, and may interact extensively with other microorganisms in situ.

GIS, Spatial Analysis, and Modeling

2019 ◽  
pp. 1284-1297
Author(s):  
Khadijeh Rouzbehani ◽  
Ghazaleh Sajjadi ◽  
Mohamad Rahim Hatami
Keyword(s):  
Breast Cancer ◽  
United States ◽  
At Risk ◽  
The United States ◽  
Background Information ◽  
Health Related Research ◽  
Health Related ◽  
Physical Environmental Factors ◽  
International Strategies

Breast cancer is a major health issue in all countries affecting thousands of women. Its causes are unknown and the national and international strategies to reduce its morbidity and mortality levels are based on early detection of cancer through screening and treatment according to clinical guidelines. Thus, knowledge of which women are at risk and why they are at risk is therefore essential component of disease prevention and screening. In 2015, an estimated 231,840 new cases of invasive breast cancer are expected to be diagnosed in women in the United States, along with 60,290 new cases of non-invasive (in situ) breast cancer. The purpose of this study is to provide a more detailed analysis of the breast cancer distribution in the United States by comparing the spatial distribution of breast cancer cases against physical environmental factors using Geographic Information System (GIS). Further, it gives background information to the GIS and its applications in health-related research.

GIS, Spatial Analysis, and Modeling

2017 ◽  
pp. 16-29
Author(s):  
Khadijeh Rouzbehani ◽  
Ghazaleh Sajjadi ◽  
Mohamad Rahim Hatami
Keyword(s):  
Breast Cancer ◽  
United States ◽  
At Risk ◽  
The United States ◽  
Background Information ◽  
Health Related Research ◽  
Health Related ◽  
Physical Environmental Factors ◽  
International Strategies

Breast cancer is a major health issue in all countries affecting thousands of women. Its causes are unknown and the national and international strategies to reduce its morbidity and mortality levels are based on early detection of cancer through screening and treatment according to clinical guidelines. Thus, knowledge of which women are at risk and why they are at risk is therefore essential component of disease prevention and screening. In 2015, an estimated 231,840 new cases of invasive breast cancer are expected to be diagnosed in women in the United States, along with 60,290 new cases of non-invasive (in situ) breast cancer. The purpose of this study is to provide a more detailed analysis of the breast cancer distribution in the United States by comparing the spatial distribution of breast cancer cases against physical environmental factors using Geographic Information System (GIS). Further, it gives background information to the GIS and its applications in health-related research.

Efficacy of soluble sodium tripolyphosphate amendments for the in-situ immobilisation of uranium

2007 ◽  
Vol 4 (5) ◽  
pp. 293 ◽  
Author(s):  
Dawn M. Wellman ◽  
Eric M. Pierce ◽  
Michelle M. Valenta
Keyword(s):  
United States ◽  
Drinking Water ◽  
Sodium Tripolyphosphate ◽  
The United States ◽  
Water Soluble ◽  
Column Experiments ◽  
Site Specific ◽  
Water Saturated ◽  
Phosphate Compounds

Environmental context. Contamination of groundwater and sediments by heavy metals and radioactive metals is a significant problem within the United States Department of Energy complex as a result of past nuclear operations. One way to remediate these metals is through reaction with phosphate compounds, which can immobilise the metals by forming highly stable metal phosphate compounds. Long-chain, water-soluble phosphate compounds provide a means to inject phosphate into subsurface contaminant plumes, to precipitate metal ions from solution. Results presented here illustrate that application of a soluble sodium tripolyphosphate to sediment contaminated with uranium will rapidly reduce the concentration of uranium in the pore water to concentrations near or below drinking water limits under water-saturated and unsaturated conditions. Abstract. A series of conventional water-saturated and pressurised unsaturated flow column experiments were conducted to evaluate the effects of using soluble polyphosphate amendments for in-situ, subsurface remediation of uranium. Experiments were conducted under mildly alkaline, calcareous conditions, representative of conditions commonly encountered at sites across the arid western United States. Results presented here illustrate that application of a soluble polyphosphate amendment to sediment contaminated with uranium will rapidly reduce the concentration of uranium released to the porewater to near or below drinking water limits under water-saturated and -unsaturated conditions. Column experiments conducted in the absence of polyphosphate illustrate sustained release of aqueous uranium at concentrations well above drinking water standards in excess of over 25 pore volumes under water-saturated conditions and over 50 pore volumes under unsaturated conditions. In the presence of tripolyphosphate, the concentration of aqueous uranium released from the sediment was below drinking water limits within 10 to 35 pore volumes under water-saturated and unsaturated conditions, respectively. Moreover, results indicate the necessity of conducting site-specific dynamic tests to tailor phosphate-based remediation technology to site specific geochemical and hydrological conditions.

An Overview of US EPA’s Current Radioactive Waste Management and General Radiation Protection Efforts

2009 ◽  
Author(s):  
R. Thomas Peake ◽  
Daniel Schultheisz ◽  
Loren W. Setlow ◽  
Brian Littleton ◽  
Reid Rosnick ◽  
...  
Keyword(s):  
United States ◽  
Radioactive Waste ◽  
Radiation Protection ◽  
Nuclear Power ◽  
Fuel Cycle ◽  
The United States ◽  
Uranium Mining ◽  
Radioactive Waste Disposal ◽  
Environmental Standards

The United States Environmental Protection Agency’s (EPA) Radiation Protection Division is the portion of EPA (or the Agency) that develops environmental standards for radioactive waste disposal in the United States. One current issue of concern is the disposal of low activity radioactive waste (LAW), including wastes that would be produced by a radiological dispersal device (RDD), for which current disposal options may be either inconsistent with the hazard presented by the material or logistically problematic. Another major issue is related to the resurgence in uranium mining. Over the past several years, demand for uranium for nuclear power plant fuel has increased as has the price. The increase in price has made uranium mining potentially profitable in the US. EPA is reviewing its relevant regulations, developed primarily in the 1980s, for potential revisions. For example, in-situ leaching (also known as in-situ recovery) is now the technology of choice where applicable, yet our current environmental standards are focused on conventional uranium milling. EPA has two actions in process, one related to the Clean Air Act, the other related to revising the environmental standards that implement the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA). Separately, but related, EPA has developed over the last several years uranium mining documents that address technologically enhanced natural occurring radioactive materials (TENORM) from abandoned uranium mines, and wastes generated by active uranium extraction facilities. Lastly, in 1977 EPA developed environmental standards that address nuclear energy, fuel fabrication, reprocessing, and other aspects of the uranium fuel cycle. In light of the increased interest in nuclear power and the potential implementation of advanced fuel cycle technologies, the Agency is now reviewing the standards to determine their continued applicability for the twenty-first century.

Cryptic Wolbachia (Rickettsiales: Rickettsiaceae) Detection and Prevalence in Culicoides (Diptera: Ceratopogonidae) Midge Populations in the United States

2020 ◽  
Vol 57 (4) ◽  
pp. 1262-1269 ◽  
Author(s):  
Hunter Covey ◽  
Rafe H Hall ◽  
Alyssa Krafsur ◽  
Megan L Matthews ◽  
Phillip T Shults ◽  
...  
Keyword(s):  
United States ◽  
Vector Control ◽  
Disease Transmission ◽  
The United States ◽  
Hemorrhagic Disease ◽  
Human Pathogens ◽  
Control Approach ◽  
16S Gene ◽  
Culicoides Sonorensis

Abstract Culicoides midges vector numerous veterinary and human pathogens. Many of these diseases lack effective therapeutic treatments or vaccines to limit transmission. The only effective approach to limit disease transmission is vector control. However, current vector control for Culicoides midges is complicated by the biology of many Culicoides species and is not always effective at reducing midge populations and impacting disease transmission. The endosymbiont Wolbachia pipientis Hertig may offer an alternative control approach to limit disease transmission and affect Culicoides populations. Here the detection of Wolbachia infections in nine species of Culicoides midges is reported. Infections were detected at low densities using qPCR. Wolbachia infections were confirmed with the sequencing of a partial region of the 16S gene. Fluorescence in situ hybridization of Culicoides sonorensis Wirth and Jones adults and dissected ovaries confirm the presence of Wolbachia infections in an important vector of Bluetongue and Epizootic hemorrhagic disease viruses. The presence of Wolbachia in Culicoides populations in the United States suggests the need for further investigation of Wolbachia as a strategy to limit transmission of diseases vectored by Culicoides midges.

Relationships among 3 Kochia species based on PCR-generated molecular sequences and molecular cytogenetics

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 1104-1115 ◽  
Author(s):  
B S Lee ◽  
M Y Kim ◽  
R R.-C Wang ◽  
B L Waldron
Keyword(s):  
United States ◽  
In Situ Hybridization ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
5S Rdna ◽  
The United States ◽  
Cross Hybridization ◽  
Forage Kochia

Forage kochia (Kochia prostrata ssp. virescens 'Immigrant' is native to the arid and semiarid regions of central Eurasia. It was introduced into the United States in 1966 as PI 314929 and released as a perennial forage shrub in 1984. Kochia americana is a perennial native to the United States, whereas Kochia scorparia is an introduced annual species that became a weed. To assess both the breeding potential and the possibility of genetic contamination, relationships among the 3 Kochia species were analyzed using random amplified polymorphic DNA (RAPD) markers, sequence tagged site (STS) marker sequences of the chloroplast NADH dehydrogenase gene (ndhF), genomic in situ hybridization (GISH), and multicolor fluorescence in situ hybridization (MC-FISH). Seventy decamer random primers yielded 458 polymorphic bands from 9 plants of K. americana, 20 plants of K. prostrata, and 7 plants of K. scoparia. Fifty-four and 55 species-specific RAPD markers were identified for K. americana and K. prostrata, whereas 80 RAPD markers were specific to K. scoparia. Based on the presence or absence of informative RAPD markers, the 3 species always grouped into 3 distinct clusters in a NTSYSpc2.01b-generated dendrogram. The same relationships were found among the 3 Kochia species based on ndhF DNA sequence divergence. Using a set of 7 STS markers that can identify each Kochia species, we did not find a single interspecific hybrid from artificial hybridizations among the 3 Kochia species. In GISH studies, chromosomes of 1 species fluoresced in green only when they were probed by genomic DNA of the same species. Cross-hybridization by genomic DNA of another species was not observed. In FISH studies using pTa71 (for 18S–5.8S–26S rDNAs) and pScT7 (for 5S rDNA) as probes, there were 1, 1 and 3 pTa71 sites and 2, 1, and 1 pScT7 sites in each haplome of K. prostrata, K. americana, and K. scoparia, respectively. It is concluded that these 3 Kochia species are so genomically distinct that gene introgression among them would be extremely rare.Key words: RAPD, STS, ndhF, GISH, FISH, mixoploidy, forage kochia.

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