Advantages of fungal enzyme production in solid state over liquid fermentation systems

2003 ◽  
Vol 13 (2-3) ◽  
pp. 157-167 ◽  
Author(s):  
Gustavo Viniegra-González ◽  
Ernesto Favela-Torres ◽  
Cristobal Noe Aguilar ◽  
Sergio de Jesus Rómero-Gomez ◽  
Gerardo Dı́az-Godı́nez ◽  
...  
Keyword(s):  
Solid State ◽  
Enzyme Production ◽  
Liquid Fermentation ◽  
Fungal Enzyme

scholarly journals Comparative Evaluation of Agroindustrial Byproducts for the Production of Alkaline Protease by Wild and Mutant Strains ofBacillus subtilisin Submerged and Solid State Fermentation

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hamid Mukhtar ◽  
Ikramul Haq
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Alkaline Protease ◽  
Enzyme Production ◽  
Enhanced Production ◽  
Industrial Byproducts ◽  
Agroindustrial Byproducts

The present study describes the screening of different agroindustrial byproducts for enhanced production of alkaline protease by a wild and EMS induced mutant strain ofBacillus subtilisIH-72EMS8. During submerged fermentation, different agro-industrial byproducts were tested which include defatted seed meals of rape, guar, sunflower, gluten, cotton, soybean, and gram. In addition to these meals, rice bran, wheat bran, and wheat flour were also evaluated for protease production. Of all the byproducts tested, soybean meal at a concentration of 20 g/L gave maximum production of the enzyme, that is, 5.74  ±  0.26 U/mL from wild and 11.28  ±  0.45 U/mL from mutant strain, during submerged fermentation. Different mesh sizes (coarse, medium, and fine) of the soybean meal were also evaluated, and a finely ground soybean meal (fine mesh) was found to be the best. In addition to the defatted seed meals, their alkali extracts were also tested for the production of alkaline protease byBacillus subtilis, but these were proved nonsignificant for enhanced production of the enzyme. The production of the enzyme was also studied in solid state fermentation, and different agro-industrial byproducts were also evaluated for enzyme production. Wheat bran partially replaced with guar meal was found as the best substrate for maximum enzyme production under solid state fermentation conditions.

scholarly journals Screening of winery and olive mill wastes for lignocellulolytic enzyme production from Aspergillus species by solid-state fermentation

2013 ◽  
Vol 4 (3) ◽  
pp. 201-209 ◽  
Author(s):  
José Manuel Salgado ◽  
Luís Abrunhosa ◽  
Armando Venâncio ◽  
José Manuel Domínguez ◽  
Isabel Belo
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Aspergillus Species ◽  
Olive Mill Wastes ◽  
Olive Mill

scholarly journals Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse

2020 ◽  
pp. 2097-2116 ◽  
Author(s):  
Priscila Aparecida Casciatori Frassatto ◽  
Fernanda Perpétua Casciatori ◽  
João Cláudio Thoméo ◽  
Eleni Gomes ◽  
Maurício Boscolo ◽  
...  
Keyword(s):  
Solid State ◽  
Sugarcane Bagasse ◽  
Enzyme Production ◽  
Packed Bed ◽  
Aeration Rate ◽  
Hydrolysis Time ◽  
Enzymatic Extract ◽  
Packed Bed Bioreactor ◽  
High Concentrations ◽  
Hydrolysis Of

Cellulases are essential for the hydrolysis of lignocellulosic materials in the production of second generation ethanol. Solid-state cultivation is a process that provides high concentrations of enzymes that can be used in this hydrolysis. The objectives of this work were to produce cellulases by cultivating the fungus Myceliophthora thermophila I-1D3b in a packed bed bioreactor with sugarcane bagasse (SCB) and wheat bran (WB) as substrate and to evaluate the efficiency of the enzymatic extract in the hydrolysis of SCB in natura (BIN) and pretreated with ozone, alkali and ultrasound (BOU). The conditions for enzyme production in the bioreactor were SCB:WB at a ratio of 2.3:1 (w/w), 75 % moisture content; 45 ºC; aeration rate 240 L h-1 and 96 h. The enzyme production was evaluated by endoglucanase, xylanase, filter paper (FPU) and ?-glycosidase activities. For the application of the enzymes, a central composed response surface design with 5 repetitions of the central point was used, taking enzyme volume and hydrolysis time as factors. Such cultivation yielded the following enzymatic activities: 723 U gss-1 of endoglucanases, 2024 U gss-1 of xylanase, 12.6 U gss-1 of FPU and 41 U gss-1 of ?-glucosidase. The results of the application tests indicated the best conditions as 7.0 ml of the enzyme extract (4.2 FPU) and 6 hours for BIN and BOU. The best cellulose-glucose conversions were obtained for BOU, reaching 32.1 % at 65 ºC. In conclusion, the enzyme production in the packed bed bioreactor was efficient and BOU pretreatment improved the hydrolysis of biomass, increasing the efficiency of conversion of cellulose to glucose.

scholarly journals APROVEITAMENTO DE RESÍDUOS AGROINDUSTRIAIS: PRODUÇÃO DE ENZIMAS A PARTIR DA CASCA DE COCO VERDE

2001 ◽  
Vol 19 (1) ◽  
Author(s):  
MARIA ALICE ZARUR COELHO ◽  
SELMA GOMES FERREIRA LEITE ◽  
MORSYLEIDE DE FREITAS ROSA ◽  
ANGELA APARECIDA LEMOS FURTADO
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Industrial Production ◽  
Enzyme Production ◽  
Raw Material ◽  
Production Time ◽  
Maximum Production ◽  
E 32

Investigou-se o aproveitamento da casca do coco verde, mediante fermentação semisólida, para produção de enzimas. A casca de coco foi previamente desidratada, moída e classificada em três diferentes granulometrias, ou seja, 14, 28 e 32 mesh Tyler. Todas as enzimas obtidas tiveram sua produção máxima na faixa de 24 e 96 horas, o que corresponde ao tempo de produção industrial corrente. Cada granulometria produziu complexos enzimáticos ricos em diferentes atividades. O estudo realizado validou a hipótese do aproveitamento do resíduo da casca do coco verde na produção de enzimas por Aspergillus niger. Abstract The utilization of immature coconut peel as substrate for enzyme production by solid state fermentation was investigated. The coconut peel was previously dehydrated, milled and classified in three distinct granulometries: 14, 28 and 32 mesh Tyler. All the enzymes obtained had its maximum production in 24 to 96 hour interval, which correspond to the current industrial production time. Each granulometry produced rich enzymatic complexes with different activities. This study validates the hypothesis of benefit immature coconut peel as raw material for enzyme production by Aspergillus niger.

scholarly journals Optimization of Fungal Enzyme Production byTrichoderma harzianumKUC1716 through Surfactant-Induced Morphological Changes

Mycobiology ◽  
2017 ◽  
Vol 45 (1) ◽  
pp. 48-51 ◽  
Author(s):  
Hanbyul Lee ◽  
Young Min Lee ◽  
Young Mok Heo ◽  
Joo-Hyun Hong ◽  
Seokyoon Jang ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Morphological Changes ◽  
Fungal Enzyme
Sign in / Sign up

Export Citation Format

Share Document