Combined effect of ascorbic acid and selenium supplementation on alcohol-induced oxidative stress in guinea pigs

We examined whether prolonged marginal ascorbic acid deficiency induces oxidative stress in the retina of guinea pigs. Male guinea pigs aged four weeks were given a scorbutic diet (20 g/animal per day) with either marginally deficient ascorbic acid (0.5 mg/animal per day) or adequate ascorbic acid (1 g/animal per day) in drinking water for three and six months. The retinal contents of the reduced form of ascorbic acid in the deficient group at three and six months were 68.1 and 43.5%, respectively, of that in the corresponding adequate group. The retinal contents of the oxidized form of ascorbic acid in the deficient group at three and six months were 1.9- and 2.7-fold, respectively, higher than that in the corresponding adequate group. The content of retinal thiobarbituric acid reactive substances (TBARS), an index of lipid peroxidation, in the deficient group was 2.5-fold higher than that in the adequate group at six months. The retinal contents of reduced glutathione (GSH) in the deficient group at three and six months were 84.8 and 66.7%, respectively, of that in the corresponding adequate group. The deficient group had 37.5% of retinal vitamin E content of the adequate group at six months. The deficient group had higher serum vitamin E concentration than the adequate group in both experimental periods. There were no differences in serum TBARS and GSH concentrations between the groups at both periods. These results indicate that prolonged marginal ascorbic acid deficiency induces oxidative stress in the retina of guinea pigs without systemic oxidative stress.

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Ascorbic acid supplementation down-regulates the alcohol induced oxidative stress, hepatic stellate cell activation, cytotoxicity and mRNA levels of selected fibrotic genes in guinea pigs

Free Radical Research ◽

10.3109/10715762.2011.647691 ◽

2012 ◽

Vol 46 (2) ◽

pp. 204-213 ◽

Cited By ~ 25

Author(s):

P. A. Abhilash ◽

R. Harikrishnan ◽

M. Indira

Keyword(s):

Oxidative Stress ◽

Ascorbic Acid ◽

Hepatic Stellate Cell ◽

Guinea Pigs ◽

Cell Activation ◽

Stellate Cell ◽

Mrna Levels ◽

Acid Supplementation ◽

Hepatic Stellate Cell Activation ◽

Ascorbic Acid Supplementation

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Combined effect of ascorbic acid deficiency and underfeeding on the hepatic carnitine level in guinea-pigs

Cellular and Molecular Life Sciences ◽

10.1007/bf01965181 ◽

1983 ◽

Vol 39 (5) ◽

pp. 512-513 ◽

Cited By ~ 14

Author(s):

A. Sandor ◽

Gy. Kispal ◽

J. Kerner ◽

I. Alkonyi

Keyword(s):

Ascorbic Acid ◽

Guinea Pigs ◽

Combined Effect ◽

Carnitine Level ◽

Ascorbic Acid Deficiency ◽

Acid Deficiency

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Short-term ascorbic acid deficiency induced oxidative stress in the retinas of young guinea pigs

Journal of Biomedical Science ◽

10.1007/bf02256560 ◽

2004 ◽

Vol 11 (2) ◽

pp. 172-178 ◽

Cited By ~ 1

Author(s):

Yoshiji Ohta ◽

Taku Okubo ◽

Takafumi Niwa ◽

Takashi Yamasaki ◽

Masayuki Horiguchi

Keyword(s):

Oxidative Stress ◽

Ascorbic Acid ◽

Guinea Pigs ◽

Short Term ◽

Ascorbic Acid Deficiency ◽

Acid Deficiency

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Effect of Exposure to Mixture of Heavy Metals Arsenic, Cadmium and Lead on Reproductive Function and Oxidative Stress in Female Rats

INTERNATIONAL JOURNAL OF TOXICOLOGICAL AND PHARMACOLOGICAL RESEARCH ◽

10.25258/ijtpr.v9i03.9075 ◽

2017 ◽

Vol 9 (03) ◽

Author(s):

Choudhuri D. ◽

Bhattacharjee T.

Keyword(s):

Oxidative Stress ◽

Heavy Metals ◽

Ascorbic Acid ◽

Reproductive Function ◽

Reproductive Organs ◽

Female Rats ◽

Corpora Lutea ◽

Oestrus Cycle ◽

Implantation Sites ◽

Live Fetus

Background : Toxicological consequences arising from exposure to mixtures of heavy metals especially at low, chronic and environmentally relevant doses are poorly recognised. In the present study, we evaluated effects of chronic exposure to combinations of three metals arsenic (As), cadmium (Cd) and lead (Pb) present frequently in drinking water on reproductive function and oxidative damage caused to reproductive organs of female rats. Method : Female rats were exposed to mixture of metals (As, Cdand Pb) for 90 consecutive days. The gain in body weight and weight of reproductive organs were recorded following autopsy on 91 stday. The oestrus cycle were monitored during entire treatment period. Numbers of corpora lutea, implantation sites, live fetus and survival of the fetus were evaluated in rats mated successfully with untreated male after completion of their respective treatment. Ovarian cholesterol, protein, ascorbic acid and enzyme Δ 5 -3β HSD levels were estimated. Serum levels of steroid hormones oestrogen and progesterone were estimated. Histopathological picture of both ovary and uterus were assessed. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidise (GPX) activity, amount of reduced glutathione (GSH) and malondyaldehyde (MDA) in blood, ovary and uterus were measured as biomarkers of oxidative stress. Results : The treated rats showed reduced body weight gain and reduction in the weight of ovary and uterus. Oestrus cycle was disrupted with continuous diestrous in treated animals. Number of corpora lutea, implantation sites and live fetus and the survival of fetus evaluate were reduced significantly in treated groups. The levels of ovarian cholesterol and ascorbic acid increased in treated rats with decrease Δ5 -3β HSD level. There was reduction in serum level of both the ovarian steroid hormones oestrogen and progesterone. The protein levels did not differ between the groups. There was a significant increase in levels of MDA and decrease in levels of all the antioxidant enzymes in treated group. Conclusion : The results revealed there was disruption to reproductive functions with decrease in stereoidogenic activity and associated oxidative stress in female rats treated with combination of mixture of metals (Cd, As and Pb) at low dose for 90 consecutive days.

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Recurrent Indoor Environmental Pollution and Its Impact on Health and Oxidative Stress of the Textile Workers in Bangladesh

Environmental Health Insights ◽

10.1177/1178630220938393 ◽

2020 ◽

Vol 14 ◽

pp. 117863022093839

Author(s):

Tania Rahman ◽

Ar-Rafi Md. Faisal ◽

Tahura Khanam ◽

Hossain Uddin Shekhar

Keyword(s):

Oxidative Stress ◽

Ascorbic Acid ◽

Occupational Health ◽

Environmental Pollution ◽

Control Area ◽

Textile Workers ◽

Control Subjects ◽

Oxidative Stresses ◽

Healthy Control ◽

Textile Industries

Perennial indoor environmental pollution in the textile industrial area is a potential health hazard for workers engaged in this line of work, resulting in mental aberration to severe health risks. This study was designed to investigate the indoor environmental quality of textile industries and correlate its effect on the occupational health and well-being of the textile workers by measuring plasma oxidative stress status in textile workers and healthy control subjects. Environmental samples were collected from 15 textile industries located in Dhaka division, and 30 volunteer textile workers and 30 volunteer office workers (control) aged 18 to 57 years participated in the study. The concentration of plasma ascorbic acid (P-ASC), plasma malondialdehyde (P-MDA), and plasma conjugated diene (P-CD) was measured in both groups. The noise level (78.0 ± 0.68 dB) and the formaldehyde level (141.80 ± 4.47 µg/m3) were found to be significantly higher in the indoor environmental area compared with those in the control area (70.17 ± 0.25 dB and 108.0 ± 0.76 µg/m3, respectively). Furthermore, the daily average concentration of suspended particulate matters (PMs), that is, PM2.5 (322.2 ± 13.46 µg/m3) and PM10 (411.0 ± 17.57 µg/m3), was also found to be significantly higher in the indoor environmental air compared with that in the control area (78.59 ± 1.66 and 174.0 ± 2.33 µg/m3, respectively). The levels of P-MDA (0.37 ± 0.03 nmol/L) and P-CD (14.74 ± 0.61 nmol/L) were significantly increased, whereas the level of P-ASC level (0.46 ± 0.04 mg/dL) was markedly decreased in the textile workers compared with the healthy control subjects (0.18 ± 0.01 nmol/L of P-MDA, 10.04 ± 0.44 nmol/L of P-CD, and 1.29 ± 0.06 mg/dL of P-ASC). The textile plants were found to have significantly elevated levels of indoor environmental pollutants compared with those in the control area, and the textile workers were significantly exposed to oxidative stresses compared with the control subjects. The use of noise pads and high-efficiency air filters is perhaps highly instrumental to put an end to this prevailing situation. Moreover, to overcome the oxidative stresses among workers, supplementation of antioxidant vitamins (ie, ascorbic acid and/or vitamin E) may be beneficial. In addition, to prevent serious health-related issues, proper precautions should be taken to protect the occupational health of the textile workers.

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