Abstract: P1173 AGE-DEPENDENT CHANGES IN LIPID METABOLISM ARE ASSOCIATED WITH CEREBROVASCULAR DISEASE AND STROKE IN POSTMENOPAUSAL WOMEN

AbstractZbtb7c is a proto-oncoprotein that controls the cell cycle and glucose, glutamate, and lipid metabolism. Zbtb7c expression is increased in the liver and white adipose tissues of aging or high-fat diet-fed mice. Knockout or knockdown of Zbtb7c gene expression inhibits the adipocyte differentiation of 3T3-L1 cells and decreases adipose tissue mass in aging mice. We found that Zbtb7c was a potent transcriptional repressor of SIRT1 and that SIRT1 was derepressed in various tissues of Zbtb7c-KO mice. Mechanistically, Zbtb7c interacted with p53 and bound to the proximal promoter p53RE1 and p53RE2 to repress the SIRT1 gene, in which p53RE2 was particularly critical. Zbtb7c induced p53 to interact with the corepressor mSin3A-HADC1 complex at p53RE. By repressing the SIRT1 gene, Zbtb7c increased the acetylation of Pgc-1α and Pparγ, which resulted in repression or activation of Pgc-1α or Pparγ target genes involved in lipid metabolism. Our study provides a molecular target that can overexpress SIRT1 protein in the liver, pancreas, and adipose tissues, which can be beneficial in the treatment of diabetes, obesity, longevity, etc.

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APOE genotypes as a risk factor for age-dependent accumulation of cerebrovascular disease in older adults

Alzheimer s & Dementia ◽

10.1016/j.jalz.2018.08.007 ◽

2018 ◽

Vol 15 (2) ◽

pp. 258-266 ◽

Cited By ~ 4

Author(s):

Melissa Lamar ◽

Lei Yu ◽

Leah H. Rubin ◽

Bryan D. James ◽

Lisa L. Barnes ◽

...

Keyword(s):

Older Adults ◽

Risk Factor ◽

Cerebrovascular Disease ◽

Age Dependent ◽

Apoe Genotypes

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Serum osteocalcin concentrations in relation to glucose and lipid metabolism in Chinese individuals

Acta Endocrinologica ◽

10.1530/eje-09-0585 ◽

2009 ◽

Vol 161 (5) ◽

pp. 723-729 ◽

Cited By ~ 128

Author(s):

Mi Zhou ◽

Xiaojing Ma ◽

Huating Li ◽

Xiaoping Pan ◽

Junling Tang ◽

...

Keyword(s):

Regression Analysis ◽

Lipid Metabolism ◽

Glucose Tolerance ◽

Lipid Profile ◽

Postmenopausal Women ◽

Multiple Logistic Regression Analysis ◽

Premenopausal Women ◽

Diabetic Patients ◽

Model Assessment ◽

Serum Osteocalcin

ObjectivesOsteocalcin, a bone-derived protein, has recently been reported to affect energy metabolism. We investigated the relationship between serum osteocalcin and parameters of adiposity, glucose tolerance, and lipid profile in Chinese subjects.MethodsSerum osteocalcin was measured by electrochemiluminescence immunoassay in 254 men (128 with newly diagnosed type 2 diabetes mellitus (T2DM) and 126 with normal glucose tolerance (NGT)), 66 premenopausal women (33 with T2DM and 33 with NGT) as well as 180 postmenopausal women (92 with T2DM and 88 with NGT). Their associations with parameters of adiposity, glucose tolerance, and lipid profile were examined.ResultsSerum osteocalcin concentrations in diabetic patients were significantly lower than those in NGT subjects after adjusted for age, gender, and body mass index (P=0.003). Postmenopausal women had higher osteocalcin concentrations than premenopausal women and men (both P<0.001). Multiple stepwise regression analysis showed that age, %fat, high-density lipoprotein cholesterol, fasting plasma glucose, and fasting serum insulin were independently associated with osteocalcin in men (P<0.05). Age and HbA1c were independently correlated with osteocalcin in postmenopausal women. Besides age and HbA1c, serum triglyceride was also an independent factor influencing osteocalcin in premenopausal women. In addition, osteocalcin was also positively associated with homeostasis model assessment of β-cell function. Furthermore, multiple logistic regression analysis demonstrated that osteocalcin was independently associated with T2DM.ConclusionsSerum osteocalcin was closely associated with not only fat and glucose metabolism but also with lipid metabolism.

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Associations between B vitamin and amino acid intake, MTHFR genotype, atherogenic indices, and homocysteine levels in postmenopausal women

Proceedings of The Nutrition Society ◽

10.1017/s0029665120003201 ◽

2020 ◽

Vol 79 (OCE2) ◽

Author(s):

Agata Muzsik ◽

Agata Chmurzynska

Keyword(s):

Lipid Metabolism ◽

Amino Acid ◽

Lipid Profile ◽

Postmenopausal Women ◽

Folate Intake ◽

Vitamin B ◽

Vitamin Intake ◽

Atherogenic Indices ◽

Amino Acid Intake ◽

B Vitamin

AbstractIt has been hypothesized that insufficiency of one-carbon metabolism may aggravate lipid metabolism disturbances. Lipid metabolism abnormalities and metabolic syndrome (MetS)—which is associated with abnormally high levels of triglycerides (TG) and low levels of high-density lipoproteins (HDL)—often occur in postmenopausal women. The purpose of this study was thus to determine the relationships between amino acid and B vitamin intake, MTHFR genotype, lipid profile, and atherogenic indices in postmenopausal women.131 postmenopausal women were enrolled for the study. The mean macronutrient, amino acid, and B vitamin intake was evaluated using dietary records. Lipid profile and concentrations of apolipoprotein A1 (ApoA1) and B (ApoB) in serum were measured using the colorimetric method. The total homocysteine (tHcy) and glutathione (GSH) level in plasma were measured using high-performance liquid chromatography. MTHFR genotype (rs1801133) was determined using a single tube TaqMan SNP Genotyping Assay. We calculated atherogenic indices, such as Castelli's risk index I and II (CRI-I and CRI-II), the atherogenic coefficient (AC), and the TG/HDL ratio.No association was found between MetS and tHcy or GSH concentrations. MTHFR genotype and folate intake also did not affect tHcy, GSH, lipid profile, or atherogenic indices. Subjects with tHcy levels over the median had lower total protein intake, lower animal protein/plant protein ratio, lower protein/carbohydrates ratio, and higher amino acid intake (methionine, lysine, arginine, glycine, and tryptophan) than did subjects with lower tHcy levels. Women who failed to meet their vitamin B12 intake needs had almost 40% higher levels of tHcy than women who did met those needs.MTHFR genotype, folate intake, and tHcy levels in plasma are not associated with lipid metabolism in postmenopausal women. tHcy levels may depend on the intake of vitamin B12, and of protein or particular amino acids.The project was financed by the National Science Centre, Poland (grant number 2015/17/N/NZ9/04133).

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