Differential radiosensitisation of bladder cancer cell lines by gemcitabine and its relationship to P53 status and cell cycle perturbations

2002 ◽  
Vol 1 (1) ◽  
pp. 42
Author(s):  
Vijay Sangar ◽  
Richard Cowan ◽  
Steve Roberts ◽  
Geoff Margison ◽  
Jolyon Hendry ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bladder Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
P53 Status

Evaluation of a novel irreversible pan-HER inhibitor in bladder cancer models.

2013 ◽  
Vol 31 (6_suppl) ◽  
pp. 253-253
Author(s):  
Petros Grivas ◽  
Andreas Karatsinides ◽  
Kathleen C. Day ◽  
Priya Kunju ◽  
Alyssa Paul ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bladder Cancer ◽  
Cell Viability ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Phase Iii ◽  
Cytostatic Effect ◽  
Cancer Models

253 Background: Human epidermal receptors (HER) are overexpressed and HER signaling is biologically relevant in bladder cancer and may mediate chemotherapy resistance. Dacomitinib is a novel, potent, irreversible pan-HER inhibitor with activity against several solid tumors, currently in a phase III clinical trial in NSCLC. We hypothesized that dacomitinib has antitumor activity in bladder cancer models. Methods: Expression level of EGFR and HER2 protein was measured semi-quantitatively in 8 bladder cancer cell lines. We treated UM-UC-3, UM-UC-6, UM-UC-9 cell lines with dacomitinib (1nM-10uM) for 24-72 hours, and measured cell viability, proliferation, apoptosis, and cell cycle effects. Correlations between dose and cell viability were measured by two-way ANOVA (GraphPad Prism 5.0). We injected age-matched male NOD/SCID mice SC with 1x106 UM-UC-6 and UM-UC-9 cells, respectively, generating xenografts. Mice were randomized and treated with dacomitinib, 6mg/kg p.o. daily, starting 1 day or 1 week after cell injection; controls were treated with vehicle. Mice were monitored daily, weighed weekly, sacrificed at 4 weeks and tumors weighed. Results: In vitro, significant cytostatic effect was noted with as low as 50nM in UM-UC6 cells and 100nM in UM-UC9 cells. UM-UC3 cells did not exhibit cytostatic effect even with 1000nM, corresponding to differential target protein (HER) expression. Dacomitinib (2uM) induced apoptosis (UM-UC-6), and G1 cell cycle arrest in both cell lines. These effects corresponded to dacomitinib-mediated inhibition of EGFR, ERK, AKT phosphorylation. In vivo, xenograft weights in both cell lines were significantly lower in dacomitinib-treated mice vs control (p<0.001), corresponding to pharmacodynamic effects (decreased E-cadherin, p-EGFR, p-ERK, mitotic count). Dacomitinib 6mg/kg p.o. daily resulted in significantly lower tumor weights vs lapatinib 50 mg/kg p.o. daily in UM-UC-9 xenograft model (p=0.0052). Conclusions: Dacomitinib demonstrated single-agent activity in bladder cancer cell lines and xenografts. Induction of apoptosis and G1 phase arrest are the suggested mechanisms for anti-tumor activity. Further investigation of this inhibitor in bladder cancer models is being pursued.

Toxicogenomic activity of gemcitabine in two TP53-mutated bladder cancer cell lines: special focus on cell cycle-related genes

2012 ◽  
Vol 39 (12) ◽  
pp. 10373-10382 ◽  
Author(s):  
Glenda Nicioli da Silva ◽  
Elaine Aparecida de Camargo ◽  
Daisy Maria Favero Salvadori
Keyword(s):  
Cell Cycle ◽  
Bladder Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Special Focus

618: Identification of Apoptosis and Cell Cycle Related Genes Induced by Connexin-26 Expression in Human Bladder Cancer Cell Lines

2006 ◽  
Vol 175 (4S) ◽  
pp. 199-199
Author(s):  
Loleta D. Harris ◽  
I-Ling Lee ◽  
David Gold ◽  
Kevin Coombes ◽  
Anita Sabichi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bladder Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Connexin 26 ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Human Bladder Cancer Cell

scholarly journals Exposure to continuous bromodeoxyuridine (BrdU) differentially affects cell cycle progression of human breast and bladder cancer cell lines

2004 ◽  
Vol 37 (2) ◽  
pp. 195-206 ◽  
Author(s):  
S. Diermeier ◽  
E. Schmidt-Bruecken ◽  
M. Kubbies ◽  
L. A. Kunz-Schughart ◽  
G. Brockhoff
Keyword(s):  
Cell Cycle ◽  
Bladder Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cell Cycle Progression ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Human Breast ◽  
Cycle Progression

Effect of adenovirus-mediated hepaCAM gene expression on cell cycle of bladder cancer cell lines

2013 ◽  
Vol 33 (10) ◽  
pp. 1151-1154
Author(s):  
Chang-kun LV ◽  
Qi LIU ◽  
Fei-fei MA ◽  
Chun-li LUO ◽  
Xin XU
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Bladder Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell

CHEMOSENSITIZATION OF BLADDER CANCER CELL LINES BY HUMAN TELOMERASE REVERSE TRANSCRIPTASE ANTISENSE TREATMENT

2004 ◽  
Vol 172 (5) ◽  
pp. 2023-2028 ◽  
Author(s):  
KAI KRAEMER ◽  
SUSANNE FUESSEL ◽  
MATTHIAS KOTZSCH ◽  
SHUANGLI NING ◽  
UTA SCHMIDT ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Reverse Transcriptase ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Human Telomerase

scholarly journals Protein expression profile related to cisplatin resistance in bladder cancer cell lines detected by two-dimensional gel electrophoresis

2015 ◽  
Vol 36 (4) ◽  
pp. 253-261 ◽  
Author(s):  
Yoshinori TAOKA ◽  
Kazumasa MATSUMOTO ◽  
Kazuya OHASHI ◽  
Satoru MINAMIDA ◽  
Masahiro HAGIWARA ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Protein Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Expression Profile ◽  
Cisplatin Resistance ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Two Dimensional ◽  
Protein Expression Profile

Expression of glutathione-related enzymes in human bladder cancer cell lines

1990 ◽  
Vol 39 (11) ◽  
pp. 1817-1820 ◽  
Author(s):  
Shivendra V. Singh ◽  
Hassan Ahmad ◽  
Awtar Krishan
Keyword(s):  
Bladder Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Human Bladder Cancer Cell
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