scholarly journals Ultrasound-assisted extraction of phenolic compounds from Cratoxylum formosum ssp. formosum leaves using central composite design and evaluation of its protective ability against H2O2-induced cell death

2014 ◽  
Vol 7 ◽  
pp. S497-S505 ◽  
Author(s):  
Bancha Yingngam ◽  
Marlene Monschein ◽  
Adelheid Brantner
Keyword(s):  
Cell Death ◽  
Phenolic Compounds ◽  
Central Composite Design ◽  
Ultrasound Assisted Extraction ◽  
Protective Ability ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Central Composite

scholarly journals Ultrasound-Assisted Phytochemical Extraction Condition Optimization Using Response Surface Methodology from Perlette Grapes (Vitis vinifera)

Processes ◽  
2019 ◽  
Vol 7 (10) ◽  
pp. 749 ◽  
Author(s):  
Muhammad Kaleem ◽  
Asif Ahmad ◽  
Rai Muhammad Amir ◽  
Ghazala Kaukab Raja
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Acetic Acid ◽  
Vitis Vinifera ◽  
Central Composite Design ◽  
Flavonoid Content ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Central Composite

In the current study, bioactive compounds of Vitis vinifera (Perlette) were extracted using an ultrasound-assisted extraction technique. The central composite design of response surface methodology (RSM) was used to determine the effect of time, temperature, and concentration of acetic acid on response variables that include extract yield, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of Vitis vinifera extracts. The results of the central composite design of RSM revealed that the quadratic polynomial model is best fitted to experimental results, with all the responses having a regression coefficient greater than 0.9. Optimized extraction levels include 26.5 min, an extraction temperature of 59 °C, and an acetic acid concentration of 62.9% with good extraction yield results of 34.95 g/100 g dry weight (DW) of grapes, TPC 34.38 mg gallic acid equivalent per gram (GAE/g) DW, flavonoid content 10.21 mg quercetin equivalents per gram (QEQ/g) DW, and antioxidant activity of 9.11 mg/100 mL ascorbic acid equivalent. The present study showed that acetic acid can be effectively used for the ultrasound-assisted extraction of polyphenols, flavonoids, and antioxidants of Vitis vinifera. These optimized conditions can be used for the extraction of bioactive compounds that can be for the development of nutraceutical products.

scholarly journals OPTIMASI KONDISI EKSTRAKSI ULTRASONIKASI PADA VITAMIN C BUAH DELIMA (Punica granatum L.) MENGGUNAKAN RESPON PERMUKAAN

2021 ◽  
Vol 9 (2) ◽  
pp. 143-158
Author(s):  
S. Rosalinda ◽  
Hilda Ayu Aulia ◽  
Asri Widyasanti ◽  
Efri Mardawati
Keyword(s):  
Response Surface Methodology ◽  
Vitamin C ◽  
Central Composite Design ◽  
Response Surface ◽  
Punica Granatum ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Punica Granatum L ◽  
Central Composite

Buah Delima (Punica granatum L.) merupakan tanaman yang memiliki banyak manfaat, diantaranya memiliki kandungan antioksidan. Kandungan antioksidan dalam buah delima yang utama adalah vitamin C. Vitamin C sebagai antioksidan dibutuhkan dalam sistem imun untuk menjaga kekebalan tubuh dan juga dapat menjaga elastisitas kulit. Kandungan vitamin C dari buah delima dapat diperoleh dengan cara ekstraksi. Vitamin C tidak tahan pada suhu yang tinggi, sehingga dibutuhkan teknologi ekstraksi yang tepat. Ultrasound Assisted Extraction (UAE) merupakan ekstraksi ultrasonikasi yang dipilih karena tidak menggunakan suhu yang tinggi dan waktu ekstraksi lebih singkat. Penelitian ini bertujuan menentukan kondisi UAE optimum yang meliputi kombinasi 3 variabel, yaitu amplitudo, waktu, dan jumlah pelarut yang menghasilkan vitamin C optimum dari ekstrak daging buah delima. Tipe alat UAE yang digunakan pada penelitian ini adalah tipe Qsonica – Q500 (500 W, 20 kHz). Metode penelitian adalah eksperimental laboratorium menggunakan RSM (Response Surface Methodology) tipe CCD (Central Composite Design) dengan amplitudo minimum 40% dan maksimum 50%; waktu minimum 20 menit dan maksimum 25 menit; dan jumlah pelarut minimum 150 ml dan maksimum 225 ml. Hasil penelitian menyimpulkan bahwa banyaknya jumlah pelarut memberikan pengaruh yang nyata, sedangkan amplitudo dan lama waktu ekstraksi tidak berpengaruh nyata. Model signifikan pada nilai P < 0,05 dengan kondisi UAE optimum pada kombinasi perlakuan amplitudo 50%, waktu 25 menit, dan jumlah pelarut 150 ml yang menghasilkan nilai kadar vitamin C optimum sebesar 47,79 mg/100 gram.

Innovative Extraction Techniques for the Optimum Extraction of Phenolic Compounds from Peanut Meal and Evaluation of Their Biological Activities

2019 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Phenolic Compounds ◽  
Carcinoma Cell ◽  
Biological Activities ◽  
Peanut Meal ◽  
Ultrasound Assisted Extraction ◽  
Carcinoma Cell Lines ◽  
Assisted Extraction ◽  
Phenolic Extract ◽  
Ultrasound Assisted ◽  
Water Ratio

There is a worldwide demand for phenolic compounds (PC) because they exhibit several biological activities. This work aimed at extracting phenolic compounds from peanut meal. The methods of extraction were mainly: conventional solvent extraction (traditional methods) and ultrasound assisted extraction (recent methods) and comparing their results. Peanut meal (PM) was prepared by defatting with n-hexane, and then extracted by the two previous methods. First, the conventional solvents used were 80% methanol, ethanol, acetone, isopropanol, and distilled water. Then studied Different parameters such as meal: water ratio, also the effect of temperature and the pH on the extraction process. Second, ultrasonic assisted extractions (USAE), the parameters investigated were temperature, time and speed of sonication. Finally, all the extracts were analyzed by HPLC for their phenolic contents. Results indicated that the highest extracted PC achieved by solvents was in distilled water where 1:100, Meal: Water ratio which extracted 40 mg PC / g PM at 30& 35°C. Highest extracted PC was achieved by alkaline medium at pH 12 more than acidic and neutral medium. While (USAE) at speed 8 ultrasonication and temperature 30ᵒC, extracted 49.2mg PC /g PM. Sothe ultrasound assisted extraction exhibited great influence on the extraction of phenolic compounds from peanut meal. The ultrasonic peanut extract was examined for its antioxidant, antimicrobial and anticarcinogenic activities. The antioxidant activity of PM phenolic extract prepared by ultrasonic technique, was measured by, β-carotene, and DPPH methods, and reducing antioxidant power. Results revealed values: 84.57, 57.72 and 5960 respectively. The PM extract showed different levels of antimicrobial activity against the pathogenic bacteria used. As for the anticarcinogenic effect PM phenolic extract most effective on inhibiting colon carcinoma and lung carcinoma cell lines with IC50 = 20.7 and 20.8 µ/ml., respectively. This was followed by intestinal carcinoma and liver carcinoma cell lines with IC50= 39.6 and 40.2µ/ml.

Greener ultrasound-assisted extraction of bioactive phenolic compounds in Croton heliotropiifolius Kunth leaves

2020 ◽  
Vol 159 ◽  
pp. 105525 ◽  
Author(s):  
Cheila B. do C. de Sousa ◽  
Gilvanda L. dos Anjos ◽  
Rafaela S.A. Nóbrega ◽  
Andréia da S. Magaton ◽  
Fabrício M. de Miranda ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

scholarly journals Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

2020 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Cassiano Brown da Rocha ◽  
Caciano Pelayo Zapata Noreña
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Grape Pomace ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

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