scholarly journals Effects of different LEDs light spectrum on the growth, leaf anatomy, and chloroplast ultrastructure of potato plantlets in vitro and minituber production after transplanting in the greenhouse

2020 ◽  
Vol 19 (1) ◽  
pp. 108-119 ◽  
Author(s):  
Li-li CHEN ◽  
Kai ZHANG ◽  
Xiao-chen GONG ◽  
Hao-ying WANG ◽  
You-hui GAO ◽  
...  
Keyword(s):  
Leaf Anatomy ◽  
Chloroplast Ultrastructure ◽  
Light Spectrum ◽  
Potato Plantlets

Leaf anatomy and water loss of in vitro PEG-treated ?Valiant? grape

1995 ◽  
Vol 42 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Imed Dami ◽  
Harrison Hughes
Keyword(s):  
Leaf Anatomy ◽  
Water Loss

scholarly journals Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro culture

2020 ◽  
Vol 26 (1) ◽  
pp. 18-34 ◽  
Author(s):  
Elizangela Rodrigues Santos ◽  
João Paulo Rodrigues Martins ◽  
Luiz Carlos de Almeida Rodrigues ◽  
Andreia Barcelos Passos Lima Gontijo ◽  
Antelmo Ralph Falqueto
Keyword(s):  
Photosystem Ii ◽  
In Vitro Culture ◽  
Leaf Anatomy ◽  
Culture Medium ◽  
Stomatal Density ◽  
Photosynthetic Apparatus ◽  
Fluorescence Transient ◽  
Chlorophyll A Fluorescence Transient ◽  
Concentration Interval

Abstract When propagated in vitro, explants receive all the nutrients needed for their growth, including carbohydrates, from the culture medium. However, it is not well understood how the type and concentration of carbohydrates can affect the functioning of the photosynthetic apparatus (particularly photosystem II) of these plants. The aim was to assess the morphophysiological responses of Billbergia zebrina plants in function of sources and concentrations of carbohydrates during in vitro culture. Side shoots of plants previously established in vitro were individualized and transferred to a culture medium containing fructose, glucose or sucrose in four concentrations (0, 15, 30 or 45 g L−1). After growth for 55 days, the chlorophyll a fluorescence transient, leaf anatomy and growth were analyzed. The concentration and type of carbohydrate employed during in vitro culture did not decrease the photosynthetic apparatus performance. However, concentrations above 30 g L−1 led to anatomical modifications, revealing some degree of stress suffered by the plants. When grown in concentrations of 15 and 30 g L−1, irrespective of the carbohydrate used, the plants presented greater stomatal density. The supplementation of the culture medium with monosaccharides caused alterations in the development of the xylem vessels, such as increased number and diameter, allowing adjustment to the microenvironmental conditions. The in vitro conditions influenced the photosynthetic and anatomical responses of plants. The concentration interval from 15 to 30 g L−1 sucrose had a better effect by not causing large changes in the performance of the photosynthetic apparatus and anatomy of plants.

scholarly journals Antibacterial Activity of Blue Light against Nosocomial Wound Pathogens Growing Planktonically and as Mature Biofilms

2016 ◽  
Vol 82 (13) ◽  
pp. 4006-4016 ◽  
Author(s):  
Fenella D. Halstead ◽  
Joanne E. Thwaite ◽  
Rebecca Burt ◽  
Thomas R. Laws ◽  
Marina Raguse ◽  
...  
Keyword(s):  
Blue Light ◽  
Mammalian Cells ◽  
Wide Spectrum ◽  
Great Promise ◽  
Bacterial Biofilms ◽  
Light Spectrum ◽  
Biofilm Growth ◽  
Content Type ◽  
Elizabethkingia Meningoseptica

ABSTRACTThe blue wavelengths within the visible light spectrum are intrinisically antimicrobial and can photodynamically inactivate the cells of a wide spectrum of bacteria (Gram positive and negative) and fungi. Furthermore, blue light is equally effective against both drug-sensitive and -resistant members of target species and is less detrimental to mammalian cells than is UV radiation. Blue light is currently used for treating acnes vulgaris andHelicobacter pyloriinfections; the utility for decontamination and treatment of wound infections is in its infancy. Furthermore, limited studies have been performed on bacterial biofilms, the key growth mode of bacteria involved in clinical infections. Here we report the findings of a multicenterin vitrostudy performed to assess the antimicrobial activity of 400-nm blue light against bacteria in both planktonic and biofilm growth modes. Blue light was tested against a panel of 34 bacterial isolates (clinical and type strains) comprisingAcinetobacter baumannii,Enterobacter cloacae,Stenotrophomonas maltophilia,Pseudomonas aeruginosa,Escherichia coli,Staphylococcus aureus,Enterococcus faecium,Klebsiella pneumoniae, andElizabethkingia meningoseptica. All planktonic-phase bacteria were susceptible to blue light treatment, with the majority (71%) demonstrating a ≥5-log10decrease in viability after 15 to 30 min of exposure (54 J/cm2to 108 J/cm2). Bacterial biofilms were also highly susceptible to blue light, with significant reduction in seeding observed for all isolates at all levels of exposure. These results warrant further investigation of blue light as a novel decontamination strategy for the nosocomial environment, as well as additional wider decontamination applications.IMPORTANCEBlue light shows great promise as a novel decontamination strategy for the nosocomial environment, as well as additional wider decontamination applications (e.g., wound closure during surgery). This warrants further investigation.

Interaction between jasmonic and gibberellic acids on in vitro tuberization of potato plantlets

2000 ◽  
Vol 43 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Gabriela Castro ◽  
Guillermina Abdala ◽  
Cecilia Agüero ◽  
R. Tizio
Keyword(s):  
In Vitro Tuberization ◽  
Potato Plantlets

scholarly journals Effects of Yellow, Green, and Different Blue Spectra on Growth of Potato Plantlets In Vitro

HortScience ◽  
2018 ◽  
Vol 53 (4) ◽  
pp. 541-546 ◽  
Author(s):  
Ruining Li ◽  
Wenwen Huang ◽  
Xiaoxiao Wang ◽  
Xiaoying Liu ◽  
Zhigang Xu
Keyword(s):  
Soluble Sugar ◽  
Red Light ◽  
Green Light ◽  
Dry Weight ◽  
Light Conditions ◽  
Health Index ◽  
Yellow Light ◽  
Blade Number ◽  
Potato Plantlets

The objectives of this study were to determine the effects of yellow light (Y), green light (G), and two blue lights (B) at different wavelengths in conjunction with red light (R) on the growth and morphogenesis of potato plantlets in vitro. Randomized nodal explants were cut into 1.0–1.5 cm pieces and were grown under five different light conditions: fluorescent white light (FL); the combined spectra of R, Y, and B at 445 nm (R630B445Y); the combined spectra of R, G, and B at 445 nm (R630B445G); the combined spectra of R, Y, and B at 465 nm (R630B465Y); and the combined spectra of R, G, and B at 465 nm (R630B465G). Morphogenesis and physiological parameters were investigated. The results showed that R630B445Y and R630B465Y increased the fresh weight (FW), dry weight (DW), stem diameter, blade number, leaf area, specific leaf weight (SLW), and the health index of potato plantlets in vitro; root activity increased significantly; and soluble sugar, soluble protein, and starch also increased. The addition of Y to the combined spectra of R and B contributed to the growth, development, and morphogenesis more than the combined spectra of R and B with G, and B at 445 nm was more effective at promoting plant growth than was B at 465 nm.

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