In Silico and In Vitro Modeling Platforms for Stimulation of Emergent Processes in Neuronal Networks

SummaryIt is shown that the supernatant of unstirred whole blood at 37° C, stimulated by 1 μg/ml of collagen for 10 sec, produces a rapid generation of pro and antiaggregatory compounds with a final proaggregatory activity which can be detected for more than 60 min on a platelet rich plasma (PRP) by turbidometric aggregometry. A reversible aggregation wave that we have called BASIC wave (for Blood Aggregation Stimulatory and Inhibitory Compounds) is recorded. The collagen stimulation of unstirred PRP produces a similar but smaller BASIC wave. BASIC’s intensity increases if erythrocytes are added to PRP but decreases if white blood cells are added instead. Aspirin abolishes “ex vivo” the ability of whole blood and PRP to generate BASIC waves and dipyridamole “in vitro” significantly reduces BASIC’s intensity in whole blood in every tested sample, but shows little effect in PRP.

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DER EINFLUSS VON RESERPIN AUF DIE ANTITHYREOIDALE WIRKUNG VON KALIUMPERCHLORAT

Acta Endocrinologica ◽

10.1530/acta.0.0390423 ◽

1962 ◽

Vol 39 (3) ◽

pp. 423-430

Author(s):

H. L. Krüskemper ◽

F. J. Kessler ◽

E. Steinkrüger

Keyword(s):

Thyroid Gland ◽

Male Rats ◽

Normal Male ◽

Tissue Respiration ◽

List Type ◽

Morphological Alterations ◽

Hormone Synthesis ◽

Stimulation Of

ABSTRACT 1. Reserpine does not inhibit the tissue respiration of liver in normal male rats (in vitro). 2. The decrease of tissue respiration of the liver with simultaneous morphological stimulation of the thyroid gland after long administration of reserpine is due to a minute inhibition of the hormone synthesis in the thyroid gland. 3. The morphological alterations of the thyroid in experimental hypothyroidism due to perchlorate can not be prevented with reserpine.

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EFFECT OF ACTINOMYCIN D ON TSH-INDUCED STIMULATION OF THYROIDAL PROTEIN SYNTHESIS IN THE RAT

Acta Endocrinologica ◽

10.1530/acta.0.0770064 ◽

1974 ◽

Vol 77 (1) ◽

pp. 64-70 ◽

Cited By ~ 9

Author(s):

Gustav Wägar

Keyword(s):

Protein Synthesis ◽

Actinomycin D ◽

The Other ◽

Leucine Incorporation ◽

Short Term ◽

Other Hand ◽

Thyroid Glands ◽

Translational Level ◽

Stimulation Of

ABSTRACT Whether the short-term regulation of thyroidal protein synthesis by TSH occurs at the transcriptional or the translational level was tested by measuring the effect of actinomycin D (act D) on the TSH-induced stimulation of L-14C-leucine incorporation into the thyroidal proteins of rats. TSH was injected 6 h before the rats were killed. The thyroid glands were then removed and incubated in vitro in the presence of L-14C-leucine for 2 h. The pronounced stimulation of leucine incorporation in the TSH-treated animals was depressed as compared with controls but still significant even when the animals had been pre-treated with 100 μg act D 24 and 7 h before sacrifice. On the other hand, act D strongly decreased incorporation of 3H-uridine into RNA. Short-term regulation of thyroidal protein synthesis by TSH appears to be partly but not wholly dependent on neosynthesis of RNA. Hence regulation may partly occur at the translation level of protein synthesis.

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Stimulation of oxytocin and progesterone production by bovine granulosa and luteal cells in vitro

Acta Endocrinologica ◽

10.1530/acta.0.111s103 ◽

1986 ◽

Vol 113 (1_Suppl) ◽

pp. S103-S104 ◽

Cited By ~ 1

Author(s):

D. SCHAMS ◽

R. KOLL

Keyword(s):

Luteal Cells ◽

Progesterone Production ◽

Stimulation Of

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IN VITRO/IN SILICO CHARACTERIZATION OF ACTIVE AND PASSIVE STRESSES IN CARDIAC MUSCLE

International Journal for Multiscale Computational Engineering ◽

10.1615/intjmultcompeng.2011002352 ◽

2012 ◽

Vol 10 (2) ◽

pp. 171-188 ◽

Cited By ~ 7

Author(s):

Markus Boel ◽

Oscar J. Abilez ◽

Ahmed N Assar ◽

Christopher K. Zarins ◽

Ellen Kuhl

Keyword(s):

Cardiac Muscle ◽

In Silico ◽

In Silico Characterization

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Role of 4-O Galloylchlorogenic Acid in Lung Cancer- An Insilico Approach

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i5.8595 ◽

2017 ◽

Vol 9 (5) ◽

Author(s):

Jaynthy C. ◽

N. Premjanu ◽

Abhinav Srivastava

Keyword(s):

Lung Cancer ◽

Cell Proliferation ◽

In Silico ◽

Computational Study ◽

Regulation Mechanism ◽

Down Regulation ◽

Fruit Extract ◽

In Vitro Techniques ◽

Discovery Studio

Cancer is a major disease with millions of patients diagnosed each year with high mortality around the world. Various studies are still going on to study the further mechanisms and pathways of the cancer cell proliferation. Fucosylation is one of the most important oligosaccharide modifications involved in cancer and inflammation. In cancer development increased core fucosylation by FUT8 play an important role in cell proliferation. Down regulation of FUT8 expression may help cure lung cancer. Therefore the computational study based on the down regulation mechanism of FUT8 was mechanised. Sapota fruit extract, containing 4-Ogalloylchlorogenic acid was used as the inhibitor against FUT-8 as target and docking was performed using in-silico tool, Accelrys Discovery Studio. There were several conformations of the docked result, and conformation 1 showed 80% dock score between the ligand and the target. Further the amino acids of the inhibitor involved in docking were studied using another tool, Ligplot. Thus, in-silico analysis based on drug designing parameters shows that the fruit extract can be studied further using in-vitro techniques to know its pharmacokinetics.

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