Pseudo-outbreak ofBrevundimonas diminutaAttributed to Contamination of Culture Medium Supplement

We report an epidemiological investigation of a cluster ofBrevundimonas diminutaisolates cultured from sterile sites. Inoculation of supplement medium yielded growth ofB. diminuta. Molecular typing indicated likely contamination of the lot. NoB. diminutawas further isolated after replacement of the supplement with a new lot number.Infect Control Hosp Epidemiol2017;38:598–601

Download Full-text

Pseudo-Outbreak ofKlebsiella oxytocaSpontaneous Bacterial Peritonitis Attributed to Contamination of Multidose Vials of Culture Medium Supplement

Infection Control and Hospital Epidemiology ◽

10.1086/674857 ◽

2014 ◽

Vol 35 (2) ◽

pp. 139-143 ◽

Cited By ~ 3

Author(s):

Federico Perez ◽

Abhishek Deshpande ◽

Sirisha Kundrapu ◽

Andrea M. Hujer ◽

Robert A. Bonomo ◽

...

Keyword(s):

Blood Culture ◽

Molecular Typing ◽

Peritoneal Fluid ◽

Culture Medium ◽

Medical Center ◽

Klebsiella Oxytoca ◽

Clinical Findings ◽

Medium Supplement ◽

Cell Counts ◽

Laboratory Procedures

Objective.To determine the source of a cluster ofKlebsiella oxytocaisolates cultured from peritoneal fluid of 3 patients with cirrhosis on a single day.Design.Outbreak investigation and before-after study.Setting.A Veterans Affairs medical center.Methods.Epidemiologic investigation, analysis of antimicrobial susceptibility testing results and molecular typing ofK. oxytocaisolates with repetitive sequence-based polymerase chain reaction (rep-PCR), review of microbiology laboratory procedures for processing peritoneal fluid cultures, and comparison of peritoneal fluid contamination rates 18 months before and after modification of laboratory procedures for culturing peritoneal fluid.Results.Each of the peritoneal fluid samples that grewK. oxytocawas inoculated into blood culture bottles by different clinicians at different hospital locations. None of the patients had clinical findings suggestive of peritonitis or elevated polymorphonuclear cell counts in peritoneal fluid (range, 3-25 cells/μL). Molecular typing with rep-PCR demonstrated that theK. oxytocaisolates were genetically related (greater than 95% similarity). Laboratory procedures included the routine addition of a culture medium supplement of yeast extract and dextrose from a multidose vial into blood culture bottles with peritoneal fluid. After discontinuing use of the culture medium supplement, there was a marked reduction in the number of peritoneal fluid cultures deemed as contaminants (14.3% vs 0.9%;P<.001).Conclusion.A pseudo-outbreak ofK. oxytocaperitonitis and high rates of contamination of peritoneal fluid were attributable to contamination of a multidose culture medium supplement. This article highlights the importance of discouraging the use of multidose vials in all clinical settings.

Download Full-text

THE PREPARATION FROM HUMAN PLASMA OF A CELL CULTURE MEDIUM SUPPLEMENT THAT CAN BE PASTEURISED

Animal Cell Technology ◽

10.1016/b978-0-7506-0421-5.50033-7 ◽

1992 ◽

pp. 111-113

Author(s):

A.J. MacLeod ◽

H Hart ◽

S.J. Pelly ◽

A.A. Thompson

Keyword(s):

Cell Culture ◽

Human Plasma ◽

Culture Medium ◽

Cell Culture Medium ◽

Medium Supplement ◽

A Cell

Download Full-text

Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132005000400011 ◽

2005 ◽

Vol 48 (spe) ◽

pp. 85-95 ◽

Cited By ~ 8

Author(s):

Tássia Raffoul ◽

Kamilla Swiech ◽

Mabel Karina Arantes ◽

Álvaro Paiva Braga de Sousa ◽

Ronaldo Zucatelli Mendonça ◽

...

Keyword(s):

Performance Evaluation ◽

Culture Medium ◽

Cell Concentration ◽

Animal Cell ◽

Positive Influence ◽

Medium Supplement ◽

Co2 Environment

The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.

Download Full-text

Molecular typing and epidemiological investigation of clinical populations of Pseudomonas aeruginosa using an oligonucleotide-microarray

BMC Microbiology ◽

10.1186/1471-2180-12-152 ◽

2012 ◽

Vol 12 (1) ◽

pp. 152 ◽

Cited By ~ 17

Author(s):

Annalisa Ballarini ◽

Giovanna Scalet ◽

Malgorzata Kos ◽

Nina Cramer ◽

Lutz Wiehlmann ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Molecular Typing ◽

Oligonucleotide Microarray ◽

Epidemiological Investigation ◽

Clinical Populations

Download Full-text

Molecular typing ofCryptosporidium parvumassociated with a diarrhoea outbreak identifies two sources of exposure

Epidemiology and Infection ◽

10.1017/s0950268807009673 ◽

2007 ◽

Vol 136 (8) ◽

pp. 1147-1152 ◽

Cited By ~ 13

Author(s):

J. G. MATTSSON ◽

M. INSULANDER ◽

M. LEBBAD ◽

C. BJÖRKMAN ◽

B. SVENUNGSSON

Keyword(s):

Cryptosporidium Parvum ◽

Microsatellite Loci ◽

Molecular Typing ◽

Swimming Pool ◽

Molecular Fingerprinting ◽

Epidemiological Investigation ◽

Pool Water ◽

Pcr Products ◽

Swimming Pool Water

SUMMARYAn outbreak of cryptosporidiosis associated with exposure to outdoor swimming-pool water affected an estimated 800–1000 individuals. PCR products were obtained from faecal specimens from 30 individuals who tested positive forCryptosporidiumoocysts. RFLP and sequencing analyses showed that all individuals were infected withCryptosporidium parvum. Among the infected individuals, five had just swum in an adjacent indoor pool during the same period, and had no identified contact with individuals linked to the outdoor pool. With the use of subgenotyping based on analysis of three mini- and microsatellite loci, MS1, TP14, and GP15, we could identify two sources of exposure. One subtype was associated with the outdoor pool and another with the indoor pool. These data demonstrate that the use of mini- and microsatellite loci as markers for molecular fingerprinting ofC. parvumisolates are valuable in the epidemiological investigation of outbreaks.

Download Full-text

Comparison of serological and molecular typing methods for epidemiological investigation of Tenacibaculum species pathogenic for fish

Applied Microbiology and Biotechnology ◽

10.1007/s00253-018-8825-8 ◽

2018 ◽

Vol 102 (6) ◽

pp. 2779-2789 ◽

Cited By ~ 5

Author(s):

Clara Fernández-Álvarez ◽

Yolanda Torres-Corral ◽

Ysabel Santos

Keyword(s):

Molecular Typing ◽

Epidemiological Investigation ◽

Typing Methods

Download Full-text

Hemoglobin Extracted From Perinereis aibuhitensis Acts as a Cell Culture Medium Supplement to Reduce Apoptosis

Frontiers in Marine Science ◽

10.3389/fmars.2021.659934 ◽

2021 ◽

Vol 8 ◽

Author(s):

Qianyu Huo ◽

Jing Liu ◽

You Cheng ◽

Bin Cao ◽

Ming Lei ◽

...

Keyword(s):

Oxygen Concentration ◽

Culture Medium ◽

Oxygen Carrier ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Mass Spectrometry Analysis ◽

Binding Property ◽

Medium Supplement ◽

Apoptosis Rate ◽

Perinereis Aibuhitensis

The environmental oxygen concentration is a crucial factor affecting cell proliferation. Owing to the reversible binding property of hemoglobin to oxygen, it can be utilized to regulate the oxygen concentration in vitro, and its ability to reduce apoptosis can be evaluated. In this study, a process comprising isolation, purification, and extraction was used to obtain hemoglobin from Perinereis aibuhitensis, a polychaete invertebrate. Extracts were separated and characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Extract component identity was confirmed by matrix-assisted laser desorption time-of-flight mass spectrometry analysis, with the molecular weight determined as 412,216.6875 Da. The oxygen carrying capacity of P. aibuhitensis hemoglobin was comparable with that of human hemoglobin. P. aibuhitensis hemoglobin remarkably downregulated the apoptosis rate. Reactive oxygen species (ROS) assays confirmed the reduction in ROS production, enabling a better elucidation of the mechanism underlying the decrease in apoptosis. These results suggested that P. aibuhitensis hemoglobin is a natural oxygen carrier, that, owing to its low-cost and accessibility, can be considered a candidate for culture medium supplement to reduce the apoptosis rate.

Download Full-text

Human platelet lysate as a potential clinical-translatable supplement to support the neurotrophic properties of human adipose-derived stem cells

Stem Cell Research & Therapy ◽

10.1186/s13287-020-01949-4 ◽

2020 ◽

Vol 11 (1) ◽

Author(s):

Silvia Palombella ◽

Martino Guiotto ◽

Gillian C. Higgins ◽

Laurent L. Applegate ◽

Wassim Raffoul ◽

...

Keyword(s):

Stem Cells ◽

Growth Factor ◽

Peripheral Nerve ◽

Culture Medium ◽

Human Platelet ◽

Platelet Lysate ◽

Clinical Translation ◽

Adipose Derived Stem Cells ◽

Medium Supplement ◽

Human Platelet Lysate

Abstract Background The autologous nerve graft, despite its donor site morbidity and unpredictable functional recovery, continues to be the gold standard in peripheral nerve repair. Rodent research studies have shown promising results with cell transplantation of human adipose-derived stem cells (hADSC) in a bioengineered conduit, as an alternative strategy for nerve regeneration. To achieve meaningful clinical translation, cell therapy must comply with biosafety. Cell extraction and expansion methods that use animal-derived products, including enzymatic adipose tissue dissociation and the use of fetal bovine serum (FBS) as a culture medium supplement, have the potential for transmission of zoonotic infectious and immunogenicity. Human-platelet-lysate (hPL) serum has been used in recent years in human cell expansion, showing reliability in clinical applications. Methods We investigated whether hADSC can be routinely isolated and cultured in a completely xenogeneic-free way (using hPL culture medium supplement and avoiding collagenase digestion) without altering their physiology and stem properties. Outcomes in terms of stem marker expression (CD105, CD90, CD73) and the osteocyte/adipocyte differentiation capacity were compared with classical collagenase digestion and FBS-supplemented hADSC expansion. Results We found no significant differences between the two examined extraction and culture protocols in terms of cluster differentiation (CD) marker expression and stem cell plasticity, while hADSC in hPL showed a significantly higher proliferation rate when compared with the usual FBS-added medium. Considering the important key growth factors (particularly brain-derived growth factor (BDNF)) present in hPL, we investigated a possible neurogenic commitment of hADSC when cultured with hPL. Interestingly, hADSC cultured in hPL showed a statistically higher secretion of neurotrophic factors BDNF, glial cell-derived growth factor (GDNF), and nerve-derived growth factor (NFG) than FBS-cultured cells. When cocultured in the presence of primary neurons, hADSC which had been grown under hPL supplementation, showed significantly enhanced neurotrophic properties. Conclusions The hPL-supplement medium could improve cell proliferation and neurotropism while maintaining stable cell properties, showing effectiveness in clinical translation and significant potential in peripheral nerve research.

Download Full-text