A note on fibre number and diameter in muscles of large and small lines of mice compared at a fixed body weight

1976 ◽  
Vol 22 (1) ◽  
pp. 131-133 ◽  
Author(s):  
A. C. B. Hooper ◽  
J. C. McCarthy

SummaryM. biceps brachii and m. tibialis anterior were studied at a fixed body weight in mice selected for high and low body weight at a fixed age. Muscle weights were approximately equal in the two lines. In general, muscles from high-line mice appeared to contain a larger number of smaller fibres than low-line mice in which there were fewer but wider fibres than in the unselected control population from which the lines were derived.

1973 ◽  
Vol 17 (2) ◽  
pp. 187-196 ◽  
Author(s):  
Ignatius Byrne ◽  
J. C. Hooper ◽  
J. C. Mccarthy

SUMMARYThe effects of selection for increased and decreased body weight in mice at 5 and at 10 weeks of age on the weight and mean fibre diameter and number of seven different muscles were studied by dissecting and histologically examining animals from large, small and unselected control lines. The purpose was to learn how selection for body size affects muscle weight and its components. Selection for increased body weight significantly increased the weight of m. biceps brachii, m. tibialis anterior, m. pectoralis major, m. brachio-radialis, m. soleus, m. rectus femoris and m. psoas major. These increases in muscle weight reflected increases in both fibre diameter and number although there were a few exceptions. Selection for low body weight produced decreases in the weight of all muscles. These reflected significant reductions in mean fibre diameter and, in most cases, fibre number. Significant estimates of heterosis in mean fibre diameter were obtained in m. biceps brachii and m. tibialis anterior from crosses between the Large and Small lines selected at 5 weeks and also between the lines selected at 10 weeks of age, respectively.


1982 ◽  
Vol 47 (3) ◽  
pp. 417-431 ◽  
Author(s):  
K. S. Bedi ◽  
A. R. Birzgalis ◽  
M. Mahon ◽  
J. L. Smart ◽  
A. C. Wareham

1. Male rats were undernourished either during the geslational and suckling periods or for a period of time immediately following weaning. Some rats were killed at the end of the period of undernutrition; others were nutritionally rehabilitated for lengthy periods of time before examination. Two muscles, the extensor digitorum longus (EDL) and soleus (SOL) were studied from each rat. Histochemically-stained transverse sections of these muscles were used to determine total number of fibres, the fibre cross-sectional areas and the relative frequency of the various fibre types.2. All rats killed immediately following undernutrition showed significant deficit sin body-weight, muscle weight and fibre cross-sectional area compared to age-matched controls.3. Animals undernourished during gestation and suckling and then fed normally for 5 months showed persistent and significant deficits in body-weight, muscle weight and total fibre number. There were also significant deficits in mean fibre cross-sectional area of each fibre type except for red fibres in the EDL. No difference in the volume proportion of connective tissue was found.4. Rats undernourished after weaning and then fed ad lib. for approximately 7 months had normal body-and muscle weights. Their muscles showed no significant differences in total fibre number, relative frequency of the various fibre types, fibre size or volume proportion of connective lissue.5. These results indicate that, although the effects on rat skeletal muscle of a period of undernutrition after weaning can be rectified, undernutrition before weaning causes lasting deficits.


1966 ◽  
Vol 17 (4) ◽  
pp. 557 ◽  
Author(s):  
GH Brown ◽  
HN Turner ◽  
SSY Young ◽  
CHS Dolling

Estimates were made of the effects of the following factors on 10 fleece and body characteristics measured on breeding ewes aged 1½ to 10½ years in three mating groups over a period of 15 years: age of ewe, single or twin birth, age of dam, the ewe's own lambing performance, the year in which measurements were made, and the year in which each set of ewes was born. Two groups (S and MS) were under selection for high clean wool weight at 15–16 months, with a ceiling on wrinkle score and fibre diameter, while the third (C) was a random control. Changes with age were present in all characteristics and were similar in the three groups. The finding that selection on wool weight at an early age had no effect on subsequent age changes in any characteristic is of considerable importance. Greasy and clean wool weight reached a maximum at 34 years, then declined by 0.3–0.2 1b per year. Percentage clean yield, fibre diameter, body weight, and wrinkle score had maxima at 5½ to 6½ years. Staple length fell consistently by approximately 0.2 cm per year, while face cover rose consistently but slightly. Crimp number rose, fell, and rose again, while fibre number rose, fell, and remained constant from 4½ years. The chief source of increase in wool weight from l½ to 3½ years was an increase in the total number of fibres. The chief source of the subsequent fall was a decrease in fibre volume, with a minor contribution from a fall in total fibre number after 6½ years. Twin-born ewes cut 0.21 lb (4.2% of the mean) less clean wool per year over their lifetime than single-born ewes, while the progeny of 2-year-old ewes cut 0.32 lb (6.4%) less than the progeny of adults. The main source of lower weight in each case was a lower total fibre number. Pregnancy lowered clean wool weight more than lactation, the separate effects being 0.87 and 0.38 lb respectively (17.4 and 7.7% of the mean) and the combined effect 1.25 1b or 25.1%. Pregnancy lowered total fibre number but lactation had no further effect. Mean clean wool weights over all ages in the C group varied from year to year, the range being from 1.08 lb (21.6%)below the mean to 0.97 lb (19.4%) above. Differences in total fibre number contributed between one-third and two-thirds of the variation. Ewes born in consecutive years in the S and MS groups showed marked upward trends in clean wool weight, fibre number, and staple length, with a marked downward trend in crimp number and a slight upward trend in body weight. These trends demonstrate direct and correlated responses to the strong selection for high clean wool weight at 15–16 months of age, and the associated slight selection against fibre diameter and wrinkle score. The mean annual increases in clean wool weight were 0.15 and 0.11 Ib (3.0 and 2.2%) in the S and MS groups, approximately 40% of the increase arising from increased total fibre number and 40% from increased staple length. The effects of age and lambing performance can be used to predict productivity in flocks of differing age structures. As the casting age rises to 54 years changes in productivity are negligible. With a rise in casting age to 7½ years the average clean wool weight of the flock would fall by 0.14 lb, with a slight decrease in staple length and crimp number. These changes need to be balanced against any increased lambing percentage or decreased annual genetic gain due to increased generation interval. Comparison with other available figures indicates that age changes may vary from one area to another.


1970 ◽  
Vol 21 (6) ◽  
pp. 955 ◽  
Author(s):  
HN Turner ◽  
MG Brooker ◽  
CHS Dolling

Direct and correlated responses are reported for 10 traits in eight pairs of lines under selection for high and low values of single characteristics : clean wool weight per head, clean wool weight per unit skin area (measured only in the groups under selection for it), body weight, wrinkle score, fibre number per unit skin area (adjusted for body weight), fibre diameter, staple length, and percentage clean yield. All groups showed overall divergence in the character under selection, though response was not always symmetrical, and in two cases (long staple and low fibre number) there was no continuing response after the initial response to extreme selection of the base parents. In all except one case, whenever previous estimates of genetic correlation were at the level of 0.2 or higher, correlated responses were in the predicted direction, though not always symmetrical in magnitude. The exception was a previously reported negative correlation between fibre number and staple length, which was not exhibited under selection for either character. Where two characters of a pair were under selection, reciprocal responses agreed in sign; magnitude will be examined in a later paper. Wool per unit area was shown to have a greater influence on clean wool weight than that of surface area, with staple length on this occasion being the most important component of wool per unit area. Reasons for an apparent decrease in the relative importance of fibre number are discussed. Three other points of importance in sheep breeding are again emphasized by these results : 1. Crimp in several pairs of groups was a poor indicator of fibre diameter. 2. Increases in clean wool weight were associated with falls in crimp number, even though fibre diameter actually became finer; diameter, not crimp, should therefore be used as a guide to wool quality. 3. The absence of genetic correlations DL and NL indicates that selection can be for high N (fibre number) with high L (staple length) and low D (fibre diameter) -in other words, a desirable fleece -with no impeding genetic correlations.


1984 ◽  
Vol 51 (3) ◽  
pp. 479-483 ◽  
Author(s):  
A. C. B. Hooper

1. The dietary intake of male mice from a line selected for high body-weight was restricted to 60% by weight of the ad lib. intake of the control litter-mates between days 21 and 42 post partum.2. One group was killed and compared with controls at 42 d of age. A further group then resumed ad lib. feeding. Mice which had regained the control body-weight by 61 d of age were killed and also compared with controls.3. Muscle weight, fibre length, sarcomere length, sarcomere number per fibre, actin length and myosin length were measured in the biceps brachii and tibialis anterior muscles.4. Muscle weight, fibre length and the number of sarcomeres per fibre were significantly reduced in both muscles following dietary restriction, but regained their control values following a resumption of normal feeding. The other indices remained unchanged throughout the study.5. The pattern of fibre length changes, due entirely to alterations in sarcomere number, is similar to that reported previously during growth, senescence and immobilization and following selection for high and low body-weights.


1994 ◽  
Vol 72 (2) ◽  
pp. 173-180 ◽  
Author(s):  
Catherine M. Dwyer ◽  
Neil C. Stickland

A 60 % reduction in maternal feed intake is known to cause a reduction of approximately 20 % in biceps brachii fibre number in the guinea-pig fetus. This investigation was designed to isolate the dietary component responsible by reducing all dietary components to 60 % of the ad lib. level and supplementing the protein, carbohydrate or fat component to the level of the ad lib. intake. Fetal muscles were examined at 50 d gestation to determine numbers of primary and secondary fibres, and at term to determine total fibre number. Fetal and neonatal weights were reduced in all restricted groups (P < 0.05) when compared with ad lib. controls. At term this reduction was significantly less (P < 0.05) in the protein-supplemented group (20%) than in the 60 %-restricted and fat-supplemented groups (43%) and the carbohydrate-supplemented group (34%). Biceps brachii fibre numbers were reduced in the 60%-restricted and fat-supplemented groups by 14–16%, but fibre numbers were similar in control, protein-supplemented and carbohydrate-supplemented groups. Any reduction in fibre number was in the secondary fibre component of total fibre number. Therefore, biceps brachii fibre numbers were reduced only when maternal diets were deficient in both protein and carbohydrate.


1959 ◽  
Vol 10 (1) ◽  
pp. 97 ◽  
Author(s):  
JM Doney

Treatment of inbred lambs with crude pituitary extract produced a highly significant increase in griwth rate over the first 10 weeks when compared with untreated inbred lambs. Continued treatment from 10 to 23 weeks of age did not cause any further change in growth rate. Differences between treated and untreated lambs in body weight and size were maintained long after cessation of treatment. Wool production was significantly increased during, but not after, the treatment period. At 12 months of age (7 months' fleece), treated and untreated groups did not differ significantly in wool production, mean fibre weight, mean fibre length, or total fibre number. The ratio of secondary to primary follicles did not differ significantly at any stage. Similar treatment produced no detectable response in non-inbred lambs. It is suggested that the effect of inbreeding is partially derived from a reduction in pituitary activity. Treatment of inbred lambs caused a decrease in the variances of body weight and size and an increase in the variance of wool production. This may be explained by differences between different tissues in their hormone requirements for maximum response, and would suggest that the effects of inbreeding on the variance of any character depend on the physiological paths through which the effect is produced.


1975 ◽  
Vol 26 (2) ◽  
pp. 375 ◽  
Author(s):  
N Jackson ◽  
RE Chapman

The heritability of abnormal crimp in wool at various ages and the genetic and phenotypic cotreiations of crimp abnormality with several wool and body characters were estimated for Peppin Merino sheep. When examined by half-sib analyses of variance, the heritability of abnormal crimp scored at ages less than 4.5 years was low, whereas abnormality at 5.5 years and older was highly inherited. Heritabilities estimated by intra-sire dam-daughter regression analyses with fewer degrees of freedom did not show such a clear-cut pattern, although the estimates tended to increase with age. The genetic correlations of crimp abnormality scores at ages up to 4.5 years with scores at older ages were mainly low. Crimp abnormality scores at most ages had genetic correlations with wool and body characters at 15–16 months of age as follows: strong positive with fibre diameter, weak positive with greasy and clean wool weight, wrinkle score and staple length, and weak negative with fibre number. Genetic correlations with body weight, percentage clean yield, face cover score and crimp frequency were inconsistent. The phenotypic variance of crimp abnormality increased with age, owing almost entirely to an increase in the additive genetic variance. The environmental variance was approximately the same at all ages. Phenotypic correlations among crimp abnormality scores were generally higher between scores at close ages, and particularly at older ages. Crimp abnormality scores at all ages had positive phenotypic correlations with fibre diameter and wrinkle score and negative correlations with fibre number per unit area of skin and percentage clean yield.Crimp abnormality at old ages also had positive phenotypic correlations with greasy and clean wool weights. Environmental correlations of crimp abnormality with greasy wool weight, clean wool weight body weight and fibre number per unit area of skin were negative, and those with percentage clean yield and fibre number positive. Predicted correlated responses in crimp abnormality differed in some respects from correlated responses observed previously in groups of Peppin Merino sheep selected for high and low values of percentage clean yield, clean wool weight, fibre number per unit area of skin and fibre diameter. Methods of selection of sheep which would be expected to reduce crimp abnormality are outlined.


1973 ◽  
Vol 16 (1) ◽  
pp. 7-16 ◽  
Author(s):  
J. P. Hanrahan ◽  
A. C. Hooper ◽  
J. C. McCarthy

SUMMARYThe effects of selection for increased or decreased body weight in mice, at 5 or 10 weeks of age, on the fibre number, fibre diameter and weight of the m. sternomastoideus and m. anterior tibialis muscles were studied. Unselected control mice were also included. Significant effects were detected in the case of each of the traits. However, the magnitude of any particular correlated response to selection varied between muscles and between selection ages in an unsystematic fashion. Most of the changes in muscle weight resulting from selection were attributable to positively correlated changes in fibre number but they were also accompanied by changes in fibre diameter in some cases.The correlated responses described indicate the difficulty of predicting how selection for body weight changes will influence cellular structure of any given muscle.Phenotypic correlations between muscle fibre number and diameter were essentially zero. The phenotypic correlations between muscle weight and fibre number and fibre diameter were positive.Sex had no effect on fibre number but had an effect on fibre diameter which depended on the particular muscle and the age of the animal.


1970 ◽  
Vol 12 (4) ◽  
pp. 724-727 ◽  
Author(s):  
I. F. Carte ◽  
P. B. Siegel

Data are presented for 10 generations of selection for high and low body weight at 8 weeks of age. The response of males was greater than that of females in the high weight line, but not in the low weight line. The asymmetrical response of sexes in the high weight line was eliminated by adjustment for scaling effects. Although heritabilities were greater in the high line than in the low line, they were essentially equal for both sexes within a line.


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