Effect of phytochemicals on phase II enzyme expression in infant human primary skin fibroblast cells

Phase II metabolising enzymes enable the metabolism and excretion of potentially harmful substances in adults, but to date it is unclear whether dietary phytochemicals can induce phase II enzymes differently between adults and infants. We investigated the expression of phase II enzymes in an in vitro model of primary skin fibroblasts at three different developmental stages, 1 month, 2 years and adult, to examine potential differences in age-related phase II enzymes in response to different phytochemicals (5–20 μm) including sulphoraphane, quercetin and catechin. Following phytochemical treatment, a significant increase in mRNA of glutathione S-transferase A1 (GSTA1) and NAD(P)H:quinone oxidoreductase 1 (NQO1) was observed, with the most marked increases seen in response to sulphoraphane (3–10-fold for GSTA1, P = 0·001, and 6–35-fold for NQO1, P = 0·001–0·017). Catechin also induced 3–5-fold changes in NQO1 transcription, whereas quercetin had less effect on NQO1 mRNA induction in infant cells. Moreover, NQO1 protein levels were significantly increased in 2-year-old and adult cell models in response to sulphoraphane treatment. These results suggest that metabolic plasticity and response to xenobiotics may be different in infants and adults; and therefore the inclusion of phytochemicals in the infant diet may modulate their induction of phase II metabolism, thereby providing increased protection from potentially harmful xenobiotics in later life.

Download Full-text

NO-donating aspirin induces phase II enzymes in vitro and in vivo

Carcinogenesis ◽

10.1093/carcin/bgi262 ◽

2005 ◽

Vol 27 (4) ◽

pp. 803-810 ◽

Cited By ~ 30

Author(s):

Jianjun Gao ◽

Khosrow Kashfi ◽

Xiaoping Liu ◽

Basil Rigas

Keyword(s):

Phase Ii ◽

Phase Ii Enzymes

Download Full-text

Mutagenic activation and detoxification of benzo[a]pyrene in vitro by hepatic cytochrome P450 1A1 and phase II enzymes in three meat-producing animals

Food and Chemical Toxicology ◽

10.1016/j.fct.2010.06.026 ◽

2010 ◽

Vol 48 (8-9) ◽

pp. 2526-2531 ◽

Cited By ~ 13

Author(s):

W. Darwish ◽

Y. Ikenaka ◽

E. Eldaly ◽

M. Ishizuka

Keyword(s):

Cytochrome P450 ◽

Phase Ii ◽

Cytochrome P450 1A1 ◽

Phase Ii Enzymes ◽

Hepatic Cytochrome

Download Full-text

High-expression of ROCK1 modulates the apoptosis of lens epithelial cells in age-related cataracts by targeting p53 gene

10.21203/rs.3.rs-48570/v2 ◽

2020 ◽

Author(s):

Shanshan Hu ◽

Dongmei Su ◽

Lei Sun ◽

Zhongying Wang ◽

Lina Guan ◽

...

Keyword(s):

Epithelial Cells ◽

Protein Level ◽

P53 Protein ◽

P53 Gene ◽

Lens Epithelial Cells ◽

Protein Levels ◽

P53 Signaling ◽

Age Related ◽

Gene And Protein Expression

Abstract Background: Age-related cataract (ARC) is a serious visual impairment disease, and its pathogenesis is unclear. This article aims to investigate the role of ROCK1 in the apoptosis of lens epithelial cells (LECs) in age-related cataracts. Methods: We collect anterior capsule samples from normal people, patients with age-related cataracts, young mice and naturally aging cataract mice. The Oxidative stress-induced apoptosis model was constructed by cultivating HLE-B3 cells with H2O2. MTT, Hoechst 33342, and TUNEL assay were performed to explore proliferation and apoptosis. HE assay was used to observe cell morphology. The gene and protein expression were assessed by quantitative real-time PCR, western blot, immunofluorescence, and immunohistochemical staining.Result: The results from the clinic and mice experiments showed that the numbers of lens epithelial cells from cataract individuals were less than the control individuals. In vitro, the apoptotic cells were increased in lens epithelial cells under H2O2 treatment. The ROCK1 protein level increased in the lens epithelial cells from age-related cataract patients and the old mice, respectively. Meanwhile, the up-regulation of the ROCK1 gene was associated with H2O2-induced HLE-B3 cells apoptosis. MTT and apoptosis assay showed ROCK1 was necessary in mediating H2O2-induced lens epithelial cells apoptosis through ROCK1 over-expression and knockdown experiment, respectively. Further investigation showed that p53 protein levels had been increased during ROCK1-mediated apoptosis in response to H2O2. Besides, ROCK1 phosphorylated p53 at ser15 to up-regulate its protein level. Conclusions: This study established the novel association of ROCK1/p53 signaling with lens epithelial cells apoptosis and age-related cataract genesis.

Download Full-text

Coffees rich in chlorogenic acid or N-methylpyridinium induce chemopreventive phase II-enzymes via the Nrf2/ARE pathway in vitro and in vivo

Molecular Nutrition & Food Research ◽

10.1002/mnfr.201100115 ◽

2011 ◽

Vol 55 (5) ◽

pp. 798-802 ◽

Cited By ~ 51

Author(s):

Ute Boettler ◽

Nadine Volz ◽

Gudrun Pahlke ◽

Nicole Teller ◽

Christine Kotyczka ◽

...

Keyword(s):

Chlorogenic Acid ◽

Phase Ii ◽

Phase Ii Enzymes

Download Full-text

Xanthohumol induces phase II enzymes via Nrf2 in human hepatocytes in vitro

Toxicology in Vitro ◽

10.1016/j.tiv.2012.10.008 ◽

2013 ◽

Vol 27 (1) ◽

pp. 149-156 ◽

Cited By ~ 28

Author(s):

Violetta Krajka-Kuźniak ◽

Jarosław Paluszczak ◽

Wanda Baer-Dubowska

Keyword(s):

Phase Ii ◽

Human Hepatocytes ◽

Phase Ii Enzymes

Download Full-text

Nrf2-regulated phase II enzymes are induced by chronic ambient nanoparticle exposure in young mice with age-related impairments

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2012.02.042 ◽

2012 ◽

Vol 52 (9) ◽

pp. 2038-2046 ◽

Cited By ~ 93

Author(s):

Hongqiao Zhang ◽

Honglei Liu ◽

Kelvin J.A. Davies ◽

Constantinos Sioutas ◽

Caleb E. Finch ◽

...

Keyword(s):

Phase Ii ◽

Phase Ii Enzymes ◽

Nanoparticle Exposure ◽

Age Related ◽

Young Mice

Download Full-text

High-expression of ROCK1 modulates the apoptosis of lens epithelial cells in age-related cataracts by targeting p53 gene

Molecular Medicine ◽

10.1186/s10020-020-00251-6 ◽

2020 ◽

Vol 26 (1) ◽

Author(s):

Shanshan Hu ◽

Dongmei Su ◽

Lei Sun ◽

Zhongying Wang ◽

Lina Guan ◽

...

Keyword(s):

Epithelial Cells ◽

Protein Level ◽

P53 Protein ◽

P53 Gene ◽

Lens Epithelial Cells ◽

Protein Levels ◽

P53 Signaling ◽

Age Related ◽

Gene And Protein Expression

Abstract Background Age-related cataract (ARC) is a serious visual impairment disease, and its pathogenesis is unclear. This article aims to investigate the role of ROCK1 in the apoptosis of lens epithelial cells (LECs) in age-related cataracts. Methods We collect anterior capsule samples from normal people, patients with age-related cataracts, young mice and naturally aging cataract mice. The oxidative stress-induced apoptosis model was constructed by cultivating HLE-B3 cells with H2O2. MTT, Hoechst 33342, and TUNEL assay were performed to explore proliferation and apoptosis. HE assay was used to observe cell morphology. The gene and protein expression were assessed by quantitative real-time PCR, western blot, immunofluorescence, and immunohistochemical staining. Result The results from the clinic and mice experiments showed that the numbers of lens epithelial cells from cataract individuals were less than the control individuals. In vitro, the apoptotic cells were increased in lens epithelial cells under H2O2 treatment. The ROCK1 protein level increased in the lens epithelial cells from age-related cataract patients and the old mice, respectively. Meanwhile, the up-regulation of the ROCK1 gene was associated with H2O2-induced HLE-B3 cells apoptosis. MTT and apoptosis assay showed ROCK1 was necessary in mediating H2O2-induced lens epithelial cells apoptosis through ROCK1 over-expression and knockdown experiment, respectively. Further investigation showed that p53 protein levels had been increased during ROCK1-mediated apoptosis in response to H2O2. Besides, ROCK1 phosphorylated p53 at ser15 to up-regulate its protein level. Conclusions This study established the novel association of ROCK1/p53 signaling with lens epithelial cells apoptosis and age-related cataract genesis.

Download Full-text

Developmental changes in LH secretion by male pituitaries in vitro: from the infantile to adult period

Journal of Endocrinology ◽

10.1677/joe.0.1600333 ◽

1999 ◽

Vol 160 (3) ◽

pp. 333-341 ◽

Cited By ~ 7

Author(s):

J Bello-Pineda ◽

J Luna ◽

MC Romano ◽

ME Mendoza

Keyword(s):

Primary Cultures ◽

Male Rats ◽

Male Rat ◽

Pituitary Development ◽

Age Related ◽

Rat Pituitary ◽

Adult Cell ◽

Increased Sensitivity ◽

Lh Secretion

The secretion of LH from the anterior pituitary of male rats was studied at different periods of postnatal development. According to an established classification we used rats 14 (infantile), 23 (juvenile), 45 (pubertal) and 90 (adult) days old. By using an in vitro incubation system, both basal and stimulated LH secretion were studied in the same gland. Age-related differences were observed in basal LH secretion, with juvenile and pubertal pituitaries showing higher secretion compared with infantile and adult pituitaries. However, the GnRH-induced secretory response was significantly higher in the infantile rats than in other ages. LH secretion was also studied in primary cultures from infantile or adult pituitaries. In 24 and 48 h cultures, infantile cells showed a significantly larger response to GnRH than that of adult cells. In the infantile pituitary LH-immunopositive cells showed differences in size at different locations in the gland. At the periphery of the lobes the predominant cells were smaller and angular shaped, whereas in the center of the gland the majority of the cells were ovoid shaped. In the adult pituitary, the predominant LH-positive cells were ovoid in shape and larger in size. Furthermore, 10% more LH-positive cells were observed in infantile pituitaries. On the basis of these data we propose that at the infantile period the male rat pituitary has two populations of LH-secreting cells, one with adult secretory function and shape and a second with increased sensitivity to GnRH and with a morphology atypical of the adult cell. The results presented support the hypothesis that the infantile period is a transitional stage in the rat pituitary development.

Download Full-text

Cyclooxygenases in Rat Leydig Cells: Effects of Luteinizing Hormone and Aging

Endocrinology ◽

10.1210/en.2006-0925 ◽

2007 ◽

Vol 148 (2) ◽

pp. 735-742 ◽

Cited By ~ 20

Author(s):

Haolin Chen ◽

Lindi Luo ◽

June Liu ◽

Barry R. Zirkin

Keyword(s):

Leydig Cell ◽

Leydig Cells ◽

Close Correlation ◽

Dibutyryl Camp ◽

Protein Levels ◽

Testosterone Production ◽

Age Related ◽

The Relationship

Previous studies suggested that increased Leydig cell cyclooxygenase (COX)2 expression may be involved in the reduced testosterone production that characterizes aged Leydig cells. Our objective herein was to further elucidate the relationships among LH stimulation, Leydig cell COX2 and COX1 expression, aging, and testosterone production. Incubation of Leydig cells from young or aged rats with LH or dibutyryl cAMP resulted in increases in both intracellular COX2 protein expression and testosterone production. COX1 expression did not respond to LH or dibutyryl cAMP. Incubation of adult cells with a protein kinase A inhibitor suppressed the stimulatory effects of LH on COX2 and testosterone production. Short-term incubation of Leydig cells with TGF-α or IL-1β also increased COX2 protein levels; IGF-I had no effect. In vivo, LH also was found to stimulate both COX2 and testosterone, but not COX1. As reported previously, COX2 expression was greater in old than in young cells, and old Leydig cells responded to inhibition of COX2 in vitro with increased testosterone production. However, the effects of the COX2 inhibitors were not restricted to old cells; young Leydig cells also responded to COX2 inhibition with increased testosterone production. This and the observation that the incubation of young or old cells with LH resulted in increased COX2 and testosterone production in both cases suggests that the relationship between COX2 and testosterone production is not unique to aged Leydig cells. Moreover, the close correlation between increases in COX2 and testosterone in LH-stimulated young and aged Leydig cells is difficult to reconcile with the contention that the increased expression of COX2 in aged cells is responsible for age-related suppression of Leydig cell testosterone production.

Download Full-text