Effect of dose of Gronolone in intravaginal sponges on lambing outcome to fixed-time artificial insemination

1981 ◽  
Vol 96 (1) ◽  
pp. 253-254 ◽  
Author(s):  
P. A. Smith ◽  
M. P. Boland ◽  
I. Gordon
Keyword(s):  
Artificial Insemination ◽  
Ovarian Stimulation ◽  
Present Experiment ◽  
Intramuscular Injection ◽  
Relative Effectiveness ◽  
Fixed Time ◽  
Oestrous Cycle ◽  
High Concentration ◽  
High Degree ◽  
Pregnant Mare Serum Gonadotrophin

Research has shown that it is now possible to control the oestrous cycle of ewes with a high degree of precision (Robinson et al. 1967; Gordon, 1975; Boland, Kelleher & Gordon, 1978). There is general agreement that a high concentration of progestagen, followed by rapid withdrawal and adequate ovarian stimulation is necessary for acceptable fertility (Robinson et al. 1967; Gordon, 1975; Colas, 1975). Application of artificial insemination in France has involved the use of a 40–45 mg Cronolone sponge (Cognie, Mariana & Thimonier, 1970; Colas et al. 1973) while controlled breeding in sheep in Ireland has been used in conjunction with a 30 mg Cronolone pessary (Gordon, 1975; Smith, Boland & Gordon, 1978, 1981). The present experiment was designed to compare the relative effectiveness of two doses of Cronolone (30 or 45 mg) when administered in conjunction with two quantities(375 or 750 i.u.) of pregnant mare serum gonadotrophin (PMSG), given by intramuscular injection.

Effect of type of intravaginal progestagen on the outcome of fixed-time artificial insemination

1981 ◽  
Vol 96 (1) ◽  
pp. 243-245 ◽  
Author(s):  
P. A. Smith ◽  
M. P. Boland ◽  
I. Gordon
Keyword(s):  
Artificial Insemination ◽  
Present Experiment ◽  
Fixed Time ◽  
Field Trials ◽  
Oestrous Cycle ◽  
Conception Rates ◽  
Natural Service ◽  
Dose Levels ◽  
Pregnant Mare Serum Gonadotrophin

It is now possible to synchronize the cycle of ewes at all times of the year (Gordon, 1975). Conception rates approaching those obtained following natural service have been reported following double (Colas & Brice, 1970; Colas et al. 1973; Barlow, Pryce-Jones & Reed, 1974; Gordon, 1975) or single (Smith, Boland & Gordon, 1978) set-time inseminations. In France, synchronization of ewes' oestrous cycle is based on the use of a Cronolone pessary (Colas, 1975), while field trials conducted in Britain have used the 60 mg Medroxy progesterone acetate (MAP) sponge (Barlow et al. 1974). Robinson et al. (1967) in an evaluation of different progestagen treatments found MAP to be less predictable than Cronolone. The purpose of the present experiment therefore was to evaluate the use of a 30 mg Cronolone or a 60 mg MAP intravaginal treatment when used in conjunction with fixed-time inseminations. In addition, two dose levels of pregnant mare serum gonadotrophin (PMSG) were compared.

scholarly journals Different estrous induction methods during the non-breeding season in Kivircik ewes

2012 ◽  
Vol 51 (No. 4) ◽  
pp. 133-138 ◽  
Author(s):  
I. Dogan ◽  
Z. Nur
Keyword(s):  
Breeding Season ◽  
Medroxyprogesterone Acetate ◽  
Artificial Insemination ◽  
Intramuscular Injection ◽  
Saline Solution ◽  
Fixed Time ◽  
Sterile Saline ◽  
Time To Onset ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Fresh Semen

The efficiency of medroxyprogesterone acetate (MAP) sponges in combination with either pregnant mare serum gonadotrophin (PMSG) or cloprostenol (PGF<sub>2&alpha;</sub>) for inducing and synchronizing the estrous cycle in non-lactating Kivircik ewes was investigated during the natural non-breeding season. All ewes (n = 69) were treated with 60 mg MAP sponges for 12 days. In addition, each ewe received an intramuscular injection of either 1.5 ml sterile saline solution (n = 18); 125 &mu;g PGF<sub>2&alpha;</sub> (n = 14); 500 IU PMSG (n = 18) or 500 IU PMSG and 125 &mu;g PGF<sub>2&alpha;</sub> (n = 19), 48 h before the sponge removal. Cervical artificial insemination (AI) with diluted fresh semen was performed at a fixed time (48 and 60 h) following progestagen withdrawal. The different groups estrous response for the first 24 &plusmn; 6 h and within 120 h, time to onset and duration of the induced estrous, and pregnancy rate was found to be 36.2%, 81.6%, 41.7 &plusmn; 2.3 h, 29.6 &plusmn; 1.5 h, and 54.5%, respectively. There were significant differences between groups MAP and MAP/PGF<sub>2&alpha;</sub> and their with the two latter groups (MAP/PMSG, MAP/PMSG/PGF<sub>2&alpha;</sub>) in terms of the onset of induced estrous (P &lt; 0.05) and between groups MAP and MAP/PGF<sub>2&alpha;</sub> in terms of the duration of induced estrous (P &lt; 0.05) and between the first two groups (MAP, MAP/PGF<sub>2&alpha;</sub>) and the latter two groups (MAP/PMSG, MAP/PMSG/PGF<sub>2&alpha;</sub>) in terms of estrous response at the first 24 &plusmn; 6 h (P &lt; 0.05). These results indicate that, the use of MAP/PMSG, rather than MAP or MAP/PGF<sub>2&alpha;</sub>, was effective in the attainment of early and compact induction and synchronization of estrous in non-lactating Kivircik ewes during the natural non-breeding season.

Control of oestrus and ovulation by progesterone and cronolone administered either intramuscularly or intravaginally and subsequent fertility

1970 ◽  
Vol 21 (2) ◽  
pp. 371 ◽  
Author(s):  
PJ Holst ◽  
NW Moore
Keyword(s):  
Artificial Insemination ◽  
Intramuscular Injection ◽  
Oestrous Cycle ◽  
Subsequent Fertility ◽  
Excellent Control ◽  
Natural Service

A factorial test involving 960 cyclic Merino ewes was carried out to compare the effectiveness of two progestagens (progesterone and 17~-acetoxy-901-fluoro-11Phydroxypregn- 4-ene-3,20-dione: Cronolone) administered either by daily intramuscular injection (i/m) or by intravaginal sponge (i/vag) for the control of oestrus and ovulation. Other factors included in the test were dose of progestagen (three doses for each progestagen and method of administration), day of oestrous cycle on which treatment commenced (day 2, 6, 10, or I4), and method of mating (natural service or artificial insemination). An additional 160 untreated ewes were included as controls. Of the 960 ewes which were treated, 677 were detected in oestrus within 5 days after treatment and the onset of oestrus was earlier and more precise after intravaginal than after intramuscular treatment. There were significant effects of method of administration and of dose of progestagen on the number of ewes in which oestrus was controlled, but there were no effects of day of commencing treatment. Overall, treatment depressed fertility to both artificial insemination and natural service. Of the 677 ewes in which oestrus was controlled, 362 (54%) lambed compared with 110 of 160 control ewes (69%). However, two particular treatments, 12 mg/day progesterone by injection and 24 mg Cronolone by sponge, gave lambing performances comparable with that of control ewes. In addition both treatments gave excellent control of oestrus and ovulation.

17 INFLUENCE OF DIFFERENT CONCENTRATIONS OF PROGESTERONE DURING FIXED-TIME ARTIFICIAL INSEMINATION PROTOCOLS ON OVULATION AND PREGNANCY RATES IN NELORE HEIFERS

2008 ◽  
Vol 20 (1) ◽  
pp. 89
Author(s):  
M. F. Pegorer ◽  
R. L. Ereno ◽  
C. M. Barros
Keyword(s):  
Artificial Insemination ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
High Concentration ◽  
Serum Progesterone ◽  
Significant Difference ◽  
Intravaginal Device

Previous reports indicate that a high concentration of serum progesterone (P4) decreases LH pulse frequency (Burke et al. 1996 Anim. Reprod. Sci. 45, 13–28) and, consequently, follicular growth. This may reduce the efficiency of fixed-time artificial insemination (FTAI) protocols that utilize P4 in Bos indicus heifers (Marques et al. 2005 Acta Sci. Vet.). The objective of the present work was to evaluate the influence of different P4 concentrations on the ovulation and pregnancy rates of zebu heifers subjected to hormonal protocols for FTAI. Four hormonal protocols with different P4 concentrations were used to induce synchronization of ovulation in Nelore (Bos indicus) heifers (n = 292). Before the beginning of the treatments, the animals were subjected to ovary evaluations by ultrasonography (Aloka SSD 500, 5-MHz probe; Aloka, Tokyo, Japan) twice in an interval of 10 days. Only heifers with a corpus luteum (CL) in at least one of the ultrasonographic evaluations were utilized. At a random stage of the estrous cycle, the selected heifers received an intravaginal progesterone-releasing device containing 0.558 g of P4 [Triu�, Biogenesis, Buenos Aires, Argentina; Treatment 1 (T1); n = 76], or 1 g of P4 [Cronipress�, Biogenesis; Treatment 2 (T2); n = 70], or 0.558 g of P4 and prostaglandin F2α (PGF2α) [150 �g d-cloprostenol, IM, Croniben�, Biogenesis; Treatment 3 (T3), n = 75], or 1 g of P4 and PGF2α [Treatment 4 (T4), n = 71]. At the time of intravaginal device insertion (Day 0 = D0), all animals received 2 mg of estradiol benzoate (EB, Bioestrogen�, IM, Biogenesis), and PGF2α was administered only in animals from groups T3 and T4. Eight days later (D8), PGF2α (0.150 g d-cloprostenol) was administered to all heifers and the P4 source was removed. Twenty-four h after removal of the intravaginal device, the heifers were treated with EB (1 mg, IM), and 30 to 36 h later all animals were fixed-time inseminated (FTAI, D10), without estrus detection. In a subset of heifers from each group (T1 = 28; T2 = 34; T3 = 33; T4 = 31), ovarian ultrasonography was performed on D9 (at 8:00 A.M.) and D11 (at 14:00 P.M.) in order to determine ovulation rate after each treatment. The data were analyzed by logistic regression (GENPROC, SAS; SAS Institute, Inc., Cary, NC, USA). Ovulation rates after treatments T1, T2, T3, and T4, were, respectively, 71.4% (20/28), 76.5% (26/34), 84.9% (28/33), and 70.9% (22/31), and there was no significant difference among groups (P > 0.05). Pregnancy rates, ascertained by ultrasonography 40 days after AI, were 30.2% (23/76), 25.7% (18/70), 33.3% (25/75), and 28.2% (20/71), respectively, for T1, T2, T3, and T4, and likewise there was no significant difference among groups (P > 0.05). It is concluded that reduction of progesterone concentration in the intravaginal device (1 g v. 0.558 g) associated or not with administration of PGF2α at the beginning of treatments did not significantly influence ovulation and pregnancy rates in Nelore heifers submitted to a FTAI protocol. Additional experiments are underway to confirm or not the present results. This work was supported by FAPESP (Brazil) and BIOGENESIS (Argentina).

Induction of oestrus in ewes using a controlled internal drug release device and PMSG

1986 ◽  
Vol 106 (1) ◽  
pp. 201-203 ◽  
Author(s):  
W. M. C. Maxwell ◽  
D. R. Barnes
Keyword(s):  
Drug Release ◽  
Artificial Insemination ◽  
Day Treatment ◽  
Fixed Time ◽  
Oestrous Cycle ◽  
Release Device

A number of methods have been developed for synchronization of oestrus in sheep (reviewed by Maxwell, 1984), but the most successful have been based on the suppression of the oestrous cycle by treatment with a polyurethane progestagenimpregnated intravaginal sponge (Robinson, 1965). A precise synchronization of oestrus and ovulation for artificial insemination (A.I.) is obtained following a 12- to 14-day treatment with the intravaginal sponge and an injection of pregnant mares serum gonadotrophin (PMSG) at sponge removal (Robinson & Smith, 1967). This allows a single fixed time A.I. to be performed on all ewes without the need for detection of oestrus.

Breeding ewes using natural service or artificial insemination

1986 ◽  
Vol 1986 ◽  
pp. 67-67 ◽  
Author(s):  
Margaret E. King ◽  
Beverly J. Keeling
Keyword(s):  
Artificial Insemination ◽  
Fixed Time ◽  
Small Scale ◽  
Financial Investment ◽  
Commercial Scale ◽  
Frozen Semen ◽  
The Cost ◽  
Natural Service ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Fresh Semen

Artificial insemination of ewes has become commercially available in Britain on a small scale over the last few years. It allows the farmer to benefit from MLC recorded rams which have been selected for growth rate and more recently for carcass quality. However, proximity to an A.I. centre is an important consideration as A.I. of ewes requires the use of fresh semen. Frozen semen has not vet achieved the quality and reliability necessary on a commercial scale. Insemination is carried out at a fixed time after the removal of Intra-vaginal pessaries and an injection of Pregnant Mare Serum Gonadotrophin (PMSG). Synchronisation of oestrus using sponges and PMSG adds to the cost of using a commercial A.I. service. If A.I. is not used then one ram per ten ewes is necessary for a synchronised mating which either requires a ram sharing group or considerable financial investment if rams of equivalent quality to those used in A.I. centres are to be purchased.

scholarly journals Nutzung einer terminorientierten Besamung beim Rind auf der Grundlage einer Prostaglandin – GnRH-Behandlung

2004 ◽  
Vol 47 (1) ◽  
pp. 27-36
Author(s):  
F. Becker ◽  
E. Kanitz ◽  
F. Schneider ◽  
G. Nürnberg ◽  
W. Kanitz
Keyword(s):  
Artificial Insemination ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Oestrous Cycle ◽  
Lh Surge ◽  
Dairy Heifers ◽  
65 H ◽  
The Mean ◽  
Timed Artificial Insemination ◽  
Predetermined Time

Abstract. Title of the paper: Practical application of timed artificial insemination in cattle using a GnRH and Prostaglandin treatment Three experiments were conducted to establish a regime of oestrous synchronization and artificial insemination at a predetermined time in dairy cattle. In experiment 1, dairy heifers (n = 43) were synchronized by administering PGF2α on day 5, 8, 11 or 14 and the LH surge was recorded. Release of the LH surge ranged from 57.0 ± 8.6 h to 76.3 ± 10.0 h depending upon the day of induced luteolysis. The release of LH was followed by ovulations. The day of PGF2α administration did not affect the interval from LH peak to ovulation. In experiment 2, heifers (n = 30) were synchronized on days 8, 11 or 14 of the oestrous cycle. Animals received an injection of GnRH 65 h after induction of luteolysis. Uniform intervals from PGF2α application to LH surge were observed in animals given PGF2α on day 11 and 14. Variation for this parameter decreased also in the group of animals where luteolysis was induced on day 8. There were no significant differences due to treatment in the mean interval from LH-peak to ovulation. In experiment 3, fixed time insemination was tested on the basis of data from Experiments 1 and 2. Synchronized heifers and high yielding cows received GnRH 65 hours after PGF2α administererd between d 8 and 14 of the oestrous cycle. Artificial insemination was carried out 13 h after GnRH application. Pregnancy rates show, that fixed time artificial insemination, independent of the recorded onset of estrus can result in high pregnancy rates. Pregnancy rates in animals at different locations were higher after induction of ovulation and timed artificial insemination (79.0, 76.0 and 47.1, 53.3 %) than in control groups with insemination after detected estrus (69.8, 63.2 and 38.6, 45.0 %).

Control of oestrus and ovulation in beef cattle in central Australia

1988 ◽  
Vol 28 (1) ◽  
pp. 21 ◽  
Author(s):  
RK Munro ◽  
J Bertram
Keyword(s):  
Beef Cattle ◽  
Intramuscular Injection ◽  
Fixed Time ◽  
Gelatin Capsule ◽  
Lactating Cows ◽  
Oestradiol Benzoate ◽  
Central Australia ◽  
Calving Rate ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Hormonal Treatments

Hormonal treatments were used to control the time of oestrus and ovulation in cattle in central Australia and to observe the efficacy of several luteolytic regimes under these conditions. All animals received intravaginal progesterone (PRID) for 7-14 days with 10 mg oestradiol benzoate (ODB) in a gelatin capsule attached to each PRID and 750 i.u. pregnant mare serum gonadotrophin (PMSG) by intramuscular injection 24 h before PRID removal. In addition half the animals received 25 mg dinoprost (PGF2�) at either 0 or 24 h before PRID removal. Differences in luteolytic treatments had no significant effects on calving rates to a single fixed-time insemination of acyclic animals (52%) or cyclic animals treated with PRID for 14 days (48%). In contrast the calving rate of cyclic animals treated with PRID for 7 days was depressed in all groups except that which received PGF2, 24 h before PRID removal (18 v. 56%, P<0.025). Independent of treatment effects lactating cows had a higher calving rate than non-lacting animals (50 v. 35%, 0.05 <P<0.10). These results indicate the importance of using effective luteolytic treatments in cyclic animals where short PRID treatments are used. The most appropriate regimes for use in extensive environments would appear to be based on a 14-day PRID treatment which eliminates the need for an injection of PGF2� before PRID removal while still producing acceptable fertility.

Effect of recombinant bovine somatotropin on pregnancy rates of Nellore cows resynchronized with the use of new DIB and third use, and inseminated at fixed-time

2015 ◽  
Vol 4 (1) ◽  
pp. 356-362
Author(s):  
Josemara Silva Santos ◽  
Tania Cavalcante ◽  
Francisca Elda Ferreira Dias ◽  
Domenica Palomaris Mariano de Souza ◽  
Alencariano J.S. Falcão ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Linear Models ◽  
Plasma Concentrations ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Recombinant Bovine Somatotropin ◽  
Equine Chorionic Gonadotropin ◽  
Rectal Palpation ◽  
Intravaginal Device

The objective of the experiment was to evaluate the effects of recombinant bovine somatropin (rbST), and the reuse of the progesterone (P4) releasing devices in resynchronization, on the pregnancy rates of Nellore cows submitted to fixed-time artificial insemination. A group of 123 multiparae Nellore cows, was submitted to a resynchronization protocol: on day 0 a Bovine Intravaginal Device (DIB® ) with 1,0g of P4 was implanted, associated with intramuscular administration of 2,0mg of estradiol benzoate (IM); on day 8 DIB was removed; and 1,0mg of estradiol cypionate, 0,15mg of prostaglandin F2? and 300 UI of equine chorionic gonadotropin were administered; on day 10, fixed-time artificial insemination was conducted (FTAI). The cows were randomized into G1 (n=12) – without rbST / with used Bovine Intravaginal Device, G2 (n=50) – without rbST / with new DIB, G3 (n=11) - with rbST / with used DIB and G4 (n=50) – with rbST/ with new DIB. rbST was introduced on the eighth day of the protocol. Sixty days after TAI, pregnancy diagnoses were conducted, via rectal palpation. Blood samples were taken on day 0, 8 and 10 of the protocol, in order to assess P4 plasma concentrations. Pregnancy rates were statistically evaluated through Generalized Linear Models Theory and their significance was tested with Analysis of Deviance. Pregnancy rates were 58%, 40%, 81% and 48% for G1, G2, G3 and G4, respectively, with significant statistical difference for G3. Plasma concentrations of P4 were not statistically different among groups, or collections. In view of the results obtained, we concluded that the administration of rbST in association with P4 DIB, used for the third time, improves pregnancy rates. Estrus resynchronization and re-insemination positively impacted pregnancy rates.

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