Relationships of brown planthopper resistance to tungro virus and grain characteristics in rice

Brown planthopper, Nilaparvata lugens (Stal), is one of the most serious insect pests of rice (Oryza sativa L.) throughout Asia. Introduction of resistant varieties could be an effective means of minimizing losses from the pest. Several hundred rice cultivars resistant to the pest have been identified and the genetics of resistance has been analysed. Studies have shown that the genes for resistance to brown planthopper, bph-4, and to green leafhopper, Glh-3, are linked (Sidhu & Khush, 1979). Ideka & Kaneda (1983) reported that bph-2 for brown planthopper resistance was linked with the gene d2 for dwarfness. The present study sought to ascertain relationships between resistance to brown planthopper and tungro virus and grain characteristics.

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Genetic mapping of bph20(t) and bph21(t) loci conferring brown planthopper resistance to Nilaparvata lugens Stål in rice (Oryza sativa L.)

Euphytica ◽

10.1007/s10681-011-0437-7 ◽

2011 ◽

Vol 183 (2) ◽

pp. 161-171 ◽

Cited By ~ 16

Author(s):

L. Yang ◽

R. B. Li ◽

Y. R. Li ◽

F. K. Huang ◽

Y. Z. Chen ◽

...

Keyword(s):

Oryza Sativa ◽

Genetic Mapping ◽

Oryza Sativa L ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Brown Planthopper Resistance ◽

Nilaparvata Lugens Stål

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Determination of The Presence of Brown Planthopper Resistance Genes (Nilaparvata lugens Stål.) in Rice (Oryza sativa L.)

International Journal of Environment Agriculture and Biotechnology ◽

10.22161/ijeab.53.32 ◽

2020 ◽

Vol 5 (3) ◽

pp. 796-804

Author(s):

Pham Thi Kim Vang ◽

Nguyen Thi Lang ◽

Luong Minh Chau ◽

Tran Ngoc He

Keyword(s):

Oryza Sativa ◽

Resistance Genes ◽

Oryza Sativa L ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Brown Planthopper Resistance ◽

Nilaparvata Lugens Stål

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Overexpression of an Osa-miR162a Derivative in Rice Confers Cross-Kingdom RNA Interference-Mediated Brown Planthopper Resistance without Perturbing Host Development

International Journal of Molecular Sciences ◽

10.3390/ijms222312652 ◽

2021 ◽

Vol 22 (23) ◽

pp. 12652

Author(s):

Wenzhong Shen ◽

Shanni Cao ◽

Jinhui Liu ◽

Wenqing Zhang ◽

Jie Chen ◽

...

Keyword(s):

Rna Interference ◽

Target Genes ◽

Insect Pests ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Environmental Concerns ◽

Reproduction Process ◽

Brown Planthopper Resistance ◽

Global Population ◽

Host Development

Rice is a main food crop for more than half of the global population. The brown planthopper (BPH, Nilaparvata lugens) is one of the most destructive insect pests of rice. Currently, repeated overuse of chemical insecticides represents a common practice in agriculture for BPH control, which can induce insect tolerance and provoke environmental concerns. This situation calls for innovative and widely applicable strategies for rice protection against BPH. Here we report that the rice osa-miR162a can mediate cross-kingdom RNA interference (RNAi) by targeting the NlTOR (Target of rapamycin) gene of BPH that regulates the reproduction process. Through artificial diet or injection, osa-miR162a mimics repressed the NlTOR expression and impaired the oviposition of BPH adults. Consistently, overproduced osa-miR162a in transgenic rice plants compromised the fecundity of BPH adults fed with these plants, but meanwhile perturbed root and grain development. To circumvent this issue, we generated osa-miR162a-m1, a sequence-optimized osa-miR162a, by decreasing base complementarity to rice endogenous target genes while increasing base complementarity to NlTOR. Transgenic overexpression of osa-miR162a-m1 conferred rice resistance to BPH without detectable developmental penalty. This work reveals the first cross-kingdom RNAi mechanism in rice-BPH interactions and inspires a potentially useful approach for improving rice resistance to BPH. We also introduce an effective strategy to uncouple unwanted host developmental perturbation from desirable cross-kingdom RNAi benefits for overexpressed plant miRNAs.

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Effects of elevated CO2 on resistant and susceptible rice cultivar and its primary host, brown planthopper (BPH), Nilaparvata lugens (Stål)

Scientific Reports ◽

10.1038/s41598-021-87992-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Sengottayan Senthil-Nathan

Keyword(s):

Insect Pests ◽

Brown Planthopper ◽

Rice Cultivar ◽

Nilaparvata Lugens ◽

Carbon Accumulation ◽

Rice Cultivars ◽

Ambient Conditions ◽

Primary Host ◽

Peroxidase Enzyme ◽

Nilaparvata Lugens Stål

AbstractThe elevated CO2 (eCO2) has positive response on plant growth and negative response on insect pests. As a contemplation, the feeding pattern of the brown plant hopper, Nilaparvata lugens Stål on susceptible and resistant rice cultivars and their growth rates exposed to eCO2 conditions were analyzed. The eCO2 treatment showed significant differences in percentage of emergence and rice biomass that were consistent across the rice cultivars, when compared to the ambient conditions. Similarly, increase in carbon and decrese in nitrogen ratio of leaves and alterations in defensive peroxidase enzyme levels were observed, but was non-linear among the cultivars tested. Lower survivorship and nutritional indices of N. lugens were observed in conditions of eCO2 levels over ambient conditions. Results were nonlinear in manner. We conclude that the plant carbon accumulation increased due to eCO2, causing physiological changes that decreased nitrogen content. Similarly, eCO2 increased insect feeding, and did alter other variables such as their biology or reproduction.

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Ketahanan padi (Oryza sativa L.) varietas IPB 3S terhadap wereng batang cokelat (Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Jurnal Entomologi Indonesia ◽

10.5994/jei.17.2.97 ◽

2020 ◽

Vol 17 (2) ◽

pp. 97

Author(s):

Andi Nurdaaniyah ◽

Dadang Dadang ◽

I Wayan Winasa

Keyword(s):

Sex Ratio ◽

Insect Pests ◽

Oryza Sativa L ◽

Feeding Activity ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Population Increase ◽

Rice Varieties ◽

High Loss ◽

Rice Plants

Brown planthopper (Nilaparvata lugens (Stål)) is one of the major rice insect pests that is known to cause high loss of rice production. One of the strategies to control this pest is by using resistant rice varieties. The aim of this research was to study the resistance of IPB 3S variety to BPH by measuring BPH fecundity, sex ratio, feeding activity as well as population increase compared to Ciherang variety against brown planthopper (N. lugens). Fecundity test used a pair of BPH adult infested on 21 days after planting (DAP) of rice plants. Eggs laid in rice stalks and the rest of eggs in the ovary were counted. In order to know the change of sex ratio, a pair of BPH adults was infested on 30 DAP rice plants and allowed to lay eggs. The calculation of sex ratio was conducted at adult stage of BPH by segregation between male and female adults. Feeding activity of BPH was evaluated using ninhydrin and analyzed qualitatively using spectrophotometer. Ten fourth instar nymphs of BPH was infested on 30 DAP old of rice seedlings. Honeydew secreted by BPH was collected to filter paper sprayed with ninhydrin. Population increased test was conducted by infesting five pairs of BPH adults on 35 DAP old rice plants. Observations was conducted on nymph and adult stages. Each test was replicated 10 times. In general, IPB 3S is slightly resistant to BPH, compared to Ciherang variety. The feeding activity of BPH is higher in Ciherang compared to IPB3S. However, the fecundity and population increased of BPH in Ciherang is similar to IPB3S.

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