Scanning electron microscopy of adult Echinoparyphium recurvatum (Von Linstow, 1873) (Digenea: Echinostomatidae) from Britain

2011 ◽  
Vol 85 (4) ◽  
pp. 453-457 ◽  
Author(s):  
A.M. McCarthy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sensu Stricto ◽  
The Body ◽  
Korean Isolate ◽  
Asian Origin ◽  
Spine Shape ◽  
Old Adult ◽  
The Relationship ◽  
Scanning Electron

AbstractThe surface topography of 15-day-old adult Echinoparyphium recurvatum (Von Linstow, 1873) sensu stricto, from an isolate of the parasite utilizing Lymnaea peregra as first intermediate host in southern England, is described and illustrated using scanning electron microscopy, and is compared to those of other Echinoparyphium species from Europe, and with those of E. recurvatum of East Asian origin. The general morphology of tegumental features was found to be very similar to that of worms of the same age observed in a previous study on a Korean isolate of E. recurvatum. Comparison of collar and body spination of E. recurvatum with other 45-collar-spined members of the genus in Europe revealed some significant differences in morphology. Collar spines of E. recurvatum were found to be shorter and more pointed than those of Echinoparyphium mordwilkoi. The body spines of E. recurvatum are rounded and scale-like, extending just beyond the ventral sucker, contrasting with the pointed, thorn-shaped body spines of E. mordwilkoi, extending posteriorly to the level of the second testis. Body spine shape and distribution in E. recurvatum were found to be more similar to those of Echinoparyphium pseudorecurvatum. The value of SEM studies in elucidating the relationship between members of the genus Echinoparyphium in Britain/Europe and those in Africa, Asia and North America is suggested.

scholarly journals An unusually elongate endogeic centipede from Sardinia (Chilopoda: Geophilidae)

2016 ◽  
Author(s):  
Lucio Bonato ◽  
Marzio Zapparoli ◽  
Leandro Drago ◽  
Alessandro Minelli
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Body Surface ◽  
Sensu Stricto ◽  
The Body ◽  
European Species ◽  
Scanning Electron ◽  
Trunk Segments

Endogeophilus ichnusae gen. et sp. nov. (Chilopoda: Geophilidae sensu stricto) is described based on three specimens from two localities in south-western Sardinia, examined by light and scanning electron microscopy. The new centipede resembles the rare Ibero-Pyrenean genus Galliophilus Ribaut & Brolemann, 1927 in some features, especially in the forcipular segment, and the temperate European species Geophilus electricus (Linnaeus, 1758) in other features, especially in the ultimate leg-bearing segment. However, the true affinities of E. ichnusae gen. et sp. nov. are uncertain, because the new species departs significantly from the majority of geophilids for the higher number of legs (91–107 pairs in the specimens examined), the slender trunk segments (the sternites being longer than wide), the relatively stout legs (the tarsus being only about twice as long as wide) and the very short setae (≤ 15 mm) scattered on the body surface. All these features are probably derived and suggest adaptation to a more strictly endogeic habit than other geophilids.

scholarly journals Three-dimensional relationships between tumor cells and microcirculation with double cyanine immunolabeling, laser scanning confocal microscopy, and computer-assisted reconstruction: an alternative to cast corrosion preparations.

1994 ◽  
Vol 42 (5) ◽  
pp. 681-686 ◽  
Author(s):  
V Rummelt ◽  
L M Gardner ◽  
R Folberg ◽  
S Beck ◽  
B Knosp ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Vessels ◽  
Tumor Cells ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Melanoma Cells ◽  
Tumor Blood Vessels ◽  
The Relationship ◽  
Scanning Electron

The morphology of the microcirculation of uveal melanomas is a reliable market of tumor progression. Scanning electron microscopy of cast corrosion preparations can generate three-dimensional views of these vascular patterns, but this technique sacrifices the tumor parenchyma. Formalin-fixed wet tissue sections 100-150 microns thick from uveal melanomas were stained with the lectin Ulex europaeus agglutinin I (UEAI) and proliferating cell nuclear antigen (PCNA) to demonstrate simultaneously the tumor blood vessels and proliferating tumor cells. Indocarbocyanine (Cy3) was used as a fluorophore for UEAI and indodicarbocyanine (Cy5) was used for PCNA. Double labeled sections were examined with a laser scanning confocal microscope. Images of both stains were digitized at the same 5-microns intervals and each of the two images per interval was combined digitally to form one image. These combined images were visualized through voxel processing to study the relationship between melanoma cells expressing PCNA and various microcirculatory patterns. This technique produces images comparable to scanning electron microscopy of cast corrosion preparations while permitting simultaneous localization of melanoma cells expressing PCNA. The microcirculatory tree can be viewed from any perspective and the relationship between tumor cells and the tumor blood vessels can be studied concurrently in three dimensions. This technique is an alternative to cast corrosion preparations.

Preparation of Alcohol-Preserved Larvae of Culicidae (Diptera) for Scanning Electron Microscopy

1972 ◽  
Vol 3 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Christine Dahl
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
The Body ◽  
Taxonomic Studies ◽  
Scanning Electron ◽  
Sem Studies

AbstractA method for preparation of alcohol-preserved culicid larvae for Scanning Electron Microscope (SEM) studies is described. It is based on dehydration by ethanol-xylol and fast evaporation of xylol in +8o° C. for ten minutes. For taxonomic studies such as examination of pecten teeth, comb scales and microtrichiae in magnifications up to 6oooX the method is suitable. For studies of receptor structures on hair-tufts and microstructures of the body integument alcohol preserved material is less satisfactory. The microstructure of the comb scales is figured and their function discussed. Differences in the ultrastructure of the abdominal hair-tufts are pointed out.

scholarly journals Congenital infection with Setaria digitata and Setaria marshalli in the thoracic cavity of a Korean calf: a case report

2010 ◽  
Vol 55 (No. 6) ◽  
pp. 275-280 ◽  
Author(s):  
NS Kim ◽  
HC Kim ◽  
C. Sim ◽  
JR Ji ◽  
NS Kim ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Case Report ◽  
Congenital Infection ◽  
The Body ◽  
Thoracic Cavity ◽  
Setaria Digitata ◽  
Scanning Electron

In March 2010, a 3.5-month-old Korean native calf was anatomized and two nematode worms were detected in the thoracic cavity. The worms were identified and classified by light and scanning electron microscopy on the basis of features at the anterior and posterior parts of the worms. The worms were female Setaria digitata and Setaria marshalli and numerous eggs which contained microfilaria were detected in the uterus of both species. The body lengths of the S. digitata and S. marshalli were 78 mm and 117 mm, respectively. Mosquitoes act as the vector for Setaria nematodes but these are inactivated in winter in Korea. Therefore, we concluded that this case represented setariasis with congenital infection occurring during the summer prenatal stage of life.

scholarly journals Balantidium grimi n. sp. (Ciliophora, Litostomatea), a new species inhabiting the rectum of the frog Quasipaa spinosa from Lishui, China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 29 ◽  
Author(s):  
Weishan Zhao ◽  
Can Li ◽  
Dong Zhang ◽  
Runqiu Wang ◽  
Yingzhen Zheng ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
New Species ◽  
Body Shape ◽  
Ssu Rdna ◽  
The Body ◽  
Molecular Phylogenetic ◽  
A New Species ◽  
Scanning Electron

Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is “V”-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.

Navicula gogorevii—a new, large-celled diatom species from Vietnam (Southeast Asia)

Phytotaxa ◽  
2020 ◽  
Vol 428 (1) ◽  
pp. 60-66
Author(s):  
MAXIM KULIKOVSKIY ◽  
DMITRY CHUDAEV ◽  
ANTON GLUSHCHENKO ◽  
IRINA KUZNETSOVA ◽  
ZINAIDA KRIVOVA ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Characteristic Feature ◽  
Southeast Asia ◽  
Sensu Stricto ◽  
Diatom Species ◽  
Scanning Electron

We describe a new member from the genus Navicula Bory sensu stricto using light and scanning electron microscopy. The new species was found in Vietnam, and is a large-celled species with longitudinal grooves present on the valve exterior. Taxa sharing this feature are known from the genus Navicula, as well as the genera Haslea Simonsen, Craticula Grunow, Gyrosigma Hassall and Proschkinia Karajeva. The new species from Vietnam is compared with other, similar taxa of Navicula, and comments are presented about the systematics and biogeography of the group of Navicula species with this characteristic feature.

Composition and Microstructure of a Natural Nano-Structure Mineral: Six-Ring Rock

2011 ◽  
Vol 295-297 ◽  
pp. 869-872
Author(s):  
Qing Shan Li ◽  
Xin Wang ◽  
Jun Liu ◽  
Guang Zhong Xing
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Energy Dispersive Spectrometer ◽  
The Body ◽  
X Ray Diffraction ◽  
Nano Structure ◽  
X Ray ◽  
Transmission Electron ◽  
Nano Size ◽  
Scanning Electron

Six-ring Rock is widely used as containers of water and additives to produce health care products. In this paper, the composition and microstructure of Six-ring Rock have been investigated by using scanning electron microscopy, energy dispersive spectrometer, transmission electron microscopy, x-ray diffraction and other technologies. Results show that Six-ring Rock is composed of CaMg(CO3)2, SiO2 and KAlSi3O8. Fe atoms exist in CaMg(CO3)2 by replacing Mg atoms. Six-ring Rock shows nano-size lamellar and acerose microstructures on the surface, and nano-size monocrystals in the body. Six-ring Rock is a natural nano structure mineral.

Research Based on Scanning Electron Microscopy to Determine Original Water-Cement Ratio of Hardened Concrete

2014 ◽  
Vol 936 ◽  
pp. 1485-1489 ◽  
Author(s):  
Yue Li ◽  
Huan Tong ◽  
Jin Bo Yang ◽  
Wan Guo Dong
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Experimental Results ◽  
Hardened Concrete ◽  
Capillary Porosity ◽  
Cement Ratio ◽  
Water Cement Ratio ◽  
The Relationship ◽  
Original Water ◽  
Scanning Electron

In order to detect the influence of age on the experiment that use scanning electron microscopy (SEM) to determinate the original water-cement ratio (w/c) of hardened concrete, a series of concrete mixes are tested. Experimental results show that early ages do have an effect on the water-cement ratio of hardened concrete that determined from scanning electron microscopy, this effect will decrease while age increases. The relationship between capillary porosity, w/c and age is analyzed and a calculation equation is given.

Scanning electron microscopy of the cuticular armature of the nematode Gnathostoma spinigerum Owen, 1836 from cats in Laos

1990 ◽  
Vol 64 (3) ◽  
pp. 255-262 ◽  
Author(s):  
Tomáš Scholz ◽  
Oleg Ditrich
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Spatial Arrangement ◽  
Felis Catus ◽  
The Body ◽  
Immature Female ◽  
Gnathostoma Spinigerum ◽  
First Time ◽  
Scanning Electron

ABSTRACTThe body surface of an immature female Gnathostoma spinigerum found for the first time in the definitive host (Felis catus f. domestica) in Laos was studied using a scanning electron microscope. All types of cuticular spines, which are one of the most important features for species identification of gnathostomid nematodes, together with their spatial arrangement, are described and figured.

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