Description of two new species of the genus Thaparocleidus Jain, 1952 (Monogenea, Dactylogyridae) from freshwater fish in India: morphological and molecular phylogenetic evidence

2012 ◽  
Vol 87 (2) ◽  
pp. 160-173 ◽  
Author(s):  
A. Chaudhary ◽  
H.S. Singh
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Closely Related Species ◽  
Molecular Biological Analysis ◽  
Wallago Attu ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Secondary Structure Models ◽  
Large Subunit Rdna

AbstractThe present paper describes the taxonomy of two new monogeneans, namely, Thaparocleidus longiphallus sp. n. and T. siloniansis sp. n., based on morphological, morphometric and molecular biological analysis, collected from the fish Wallago attu (Bloch & Schn.) and Silonia silondia (Ham.), respectively, at Meerut, UP, India. Genetic characterization of the two new species is based on sequence analyses of the rDNA 28S gene using neighbour-joining and maximum-parsimony techniques. These methods are congruent in depicting T. longiphallus sp. n. and T. siloniansis sp. n. as closely related species, but distinct from each other and forming a subclade with other species of the genus Thaparocleidus Jain, 1952. Secondary-structure models of the large subunit rDNA of the two species were also predicted using a combined comparative and thermodynamic approach. Molecular morphometric and phylogenetic relationships of the isolates of the Thaparocleidus species are discussed in detail.

Molecular phylogenetic analysis reveals two new species of Discosia from Italy

Phytotaxa ◽  
2015 ◽  
Vol 203 (1) ◽  
pp. 37 ◽  
Author(s):  
Wenjing Li ◽  
Jian-kui Liu ◽  
D. Jayarama Bhat ◽  
Erio Camporesi ◽  
Dong-QING DAI ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Morphological Characters ◽  
Fruiting Bodies ◽  
Forensic Dna ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Large Subunit Rdna

Two fresh collections of Discosia were made from dead leaves of Fagus sylvatica in Italy. As these collections could not be cultured, the fruiting bodies were directly used for sequencing using a Forensic DNA Extraction Kit. Based on analyses of the concatenated internal transcribed spacer regions of the nrDNA operon (ITS) and large subunit rDNA (LSU) gene sequences, as well as morphological characters, the fresh collections are introduced as two new species, namely D. italica and D. fagi. Phylogenetically, these two species are distinct from all other Discosia species. Morphologically, D. italica is somewhat similar with D. fagi, but can be distinguished using dimension of conidiomata and conidiogenous cells. Descriptions and illustrations of the new taxa are provided herein.

Two new rock-inhabiting species of Cyphellophora from karst habitats in China

Phytotaxa ◽  
2019 ◽  
Vol 397 (1) ◽  
pp. 23
Author(s):  
WEI SUN ◽  
BINGJIE LIU ◽  
RONG FU ◽  
XINGZHONG LIU ◽  
MEICHUN XIANG
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Its Region ◽  
Closely Related Species ◽  
Two New Species ◽  
Rock Inhabiting Fungi ◽  
Karst Habitats

During survey on rock-inhabiting fungi from karst habitats in Guizhou, China, two new species in Cyphellophora were discovered and identified. Phylogenetic analysis based on combined sequences of the nuclear large subunit (nucLSU) and internal transcribed spacer (ITS) region of ribosomal DNA revealed that the tested isolates, clustered into two clades that well affiliated in the genus of Cyphellophora. Morphological characteristics were also supported the estabolishment of the new species. Herewith Cyphellophora botryose sp. nov. and Cyphellophora guizhouensis sp. nov. were described and their differences from closely related species were discussed.

Two new species of Tomentella (Thelephorales, Basidiomycota) from Lesser Xingan Mts., northeastern China

Phytotaxa ◽  
2018 ◽  
Vol 369 (2) ◽  
pp. 80 ◽  
Author(s):  
XU LU ◽  
YAN-HONG MU ◽  
HAI-SHENG YUAN
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Maximum Likelihood ◽  
Morphological Characteristics ◽  
Northeastern China ◽  
Phylogenetic Position ◽  
Closely Related Species ◽  
Molecular Analyses ◽  
Two New Species ◽  
Molecular Phylogenetic

Two new species of Tomentella from the Lesser Xingan Mts. of northeastern China, T. pallidocastanea and T. tenuirhizomorpha, are described and illustrated using morphological characteristics and molecular phylogenetic analysis. T. pallidocastanea is characterized by mucedinoid basidiocarps adherent to the substrate, a light reddish brown to pinkish grey hymenophoral surface, absence of rhizomorphs and cystidia, generative hyphae with clamps, rarely with simple septa and echinulate, and subglobose to lobed basidiospores (echinuli up to 1.5 μm long). T. tenuirhizomorpha is characterized by mucedinoid basidiocarps separable from the substrate, a greyish brown to dark brown hymenophoral surface, thin rhizomorphs in the subiculum and margins, an absence of cystidia, generative hyphae with clamps rarely with simple septa and echinulate, and subglobose to globose basidiospores (echinuli up to 1.5 μm long). Molecular analyses using Maximum Likelihood, Maximum Parsimony and Bayesian Analysis confirm the phylogenetic position of the two new species. The discriminating characters of these two new species and their closely related species are discussed in this study.

Molecular phylogenetic identification of two new brown-rot corticioid fungi in Dacryobolus from southwestern China

Phytotaxa ◽  
2016 ◽  
Vol 265 (2) ◽  
pp. 101 ◽  
Author(s):  
HAI-SHENG YUAN ◽  
YU-HE KAN ◽  
XIAN-ZHEN WAN
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Strong Support ◽  
Brown Rot ◽  
Southwestern China ◽  
Corticioid Fungi ◽  
Chongqing Municipality ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Phylogenetic Identification

Two new brown-rot corticioid fungi, Dacryobolus gracilis and D. montanus, are described from Chongqing Municipality and Guangxi Autonomous Region, southwestern China. Dacryobolus gracilis is characterized by fragile, ceraceous basidiocarps, odontioid hymenophore with slender aculei, presence of long aculeal cystidia in the central part of the aculei and narrowly allantoid basidiospores; D. montanus is characterized by resupinate, ceraceous basidiocarps, odontioid hymenophore with thicker aculei, absence of cystidia or cystidioles, and narrowly allantoid basidiospores. Molecular phylogeny inferred from internal transcribed spacer (ITS) and ribosomal large subunit (nLSU) sequences data indicated that these two new species were clustered with the other species of Dacryobolus with a strong support. These two new species are described and illustrated in this paper. A key to known species of Dacryobolus is provided.

Two new species of Barssia from China

Phytotaxa ◽  
2018 ◽  
Vol 374 (2) ◽  
pp. 129
Author(s):  
YU-YAN XU ◽  
LI-JIE GUO ◽  
TING LI ◽  
LI FAN
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
North China ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Internal Transcribed Spacer Region ◽  
Two New Species ◽  
Molecular Phylogenetic

Two new truffle species Barssia guozigouensis and B. luyashanensis are described and illustrated from North China based on morphological and molecular evidences. Morphologically, B. guozigouensis is diagnosed by its distinctly warty ascomata and solid gleba with small and irregular chambers, and B. luyashanensis is by its red brown ascomata with fine warts and gleba without chambers. Molecular phylogenetic analyses based on the ribosomal internal transcribed spacer region and 28S large subunit nrDNA supported the placement of the new species.

The genus Tremella (Tremellales, Basidiomycota) in Russia with description of two new species and proposal of one nomenclatural combination

Phytotaxa ◽  
2015 ◽  
Vol 238 (1) ◽  
pp. 40 ◽  
Author(s):  
Vera F. Malysheva ◽  
Ekaterina F. Malysheva ◽  
Eugenia M. Bulakh
Keyword(s):  
New Species ◽  
Related Species ◽  
Morphological Data ◽  
New Combination ◽  
Strongly Correlated ◽  
Closely Related Species ◽  
Phylogenetic Reconstructions ◽  
Dichotomous Key ◽  
Two New Species ◽  
Molecular Phylogenetic

Twenty-two non-lichenicolous species of Tremella are reported from Russia. Detailed descriptions, photographs, illustrations and a dichotomous key for the identification of taxa are provided. Two species, Tremella subalpina and T. griseola, are described as new. One new combination is proposed, and an epitype for Tremella yokohamensis is designated. Molecular phylogenetic reconstructions are presented based on nrITS and nrLSU regions in Bayesian and likelihood analyses. Phylogenetic data were strongly correlated with morphological data and were useful to delimit closely related species.

Two new species and a new record of Crepidotus (Agaricomycetes) from India

2020 ◽  
Author(s):  
A. Manoj Kumar ◽  
M. Catherine Aime ◽  
K. B. Vrinda ◽  
C. K. Pradeep
Keyword(s):  
New Species ◽  
Maximum Likelihood ◽  
New Record ◽  
Large Subunit ◽  
Phylogenetic Reconstruction ◽  
Rdna Sequences ◽  
Two New Species ◽  
Large Subunit Rdna ◽  
Kerala State

During ongoing studies of the mycoflora of Kerala State, India, three species of Crepidotus, namely, C. exilis A.M.Kumar & C.K.Pradeep sp. nov., C. globisporus A.M.Kumar & C.K.Pradeep sp. nov. and C. croceotinctus Peck, were discovered. These species are described and illustrated. A phylogenetic reconstruction based on maximum-likelihood analyses of nuclear ribosomal large subunit rDNA sequences are provided.

Two new species of Calocybe (Lyophyllaceae) from northeast China

Phytotaxa ◽  
2019 ◽  
Vol 425 (4) ◽  
pp. 219-232
Author(s):  
JIZE XU ◽  
XIAODONG YU ◽  
CHUNLAN ZHANG ◽  
YU LI
Keyword(s):  
New Species ◽  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Northeast China ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Liaoning Province ◽  
Two New Species ◽  
Large Subunit Rdna ◽  
Key Characters

Two new species of Calocybe (Calocybe erminea sp. nov. and C. badiofloccosa sp. nov.) are described from Liaoning province, China. Calocybe erminea is characterized mainly by its light khaki to dirty white pileus, slightly hygrophanous stipe, small basidiospores and cellular epicutis. The key characters of C. badiofloccosa are its light ocher yellow and rough pileus, villose stipe and large basidiospores. The results of phylogenetic analyses based on the internal transcribed spacer (ITS) and the nuclear large subunit rDNA (nrLSU) region indicated that they belong to Calocybe, but they are distinct from other species in the genus. They are compared morphologically with other species which are phenotypically similar and the phylogenetic relationships between them and allied taxa are discussed. These results confirm the species is new to science.

Morphological and molecular characterization of Pratylenchoides persicus n. sp. (Nematoda: Merliniidae) and additional data on two other species of the genus from Iran

Zootaxa ◽  
2016 ◽  
Vol 4205 (5) ◽  
pp. 459 ◽  
Author(s):  
KOUROSH AZIZI ◽  
ALI ESKANDARI ◽  
AKBAR KAREGAR ◽  
REZA GHADERI ◽  
SVEN VAN DEN ELSEN ◽  
...  
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Molecular Study ◽  
Closely Related Species ◽  
The Family ◽  
Lesion Nematode ◽  
Stylet Length ◽  
First Time ◽  
Morphological And Molecular Characterization

Some nematologists recently placed the genus Pratylenchoides, (“Lesion Nematode-like”) in the family Merliniidae. To investigate Pratylenchoides species diversity and their relationships with other Merliniidae genera, specimens were collected from various habitats in the northern and northwestern provinces of Iran. The morphological and molecular study yielded three species of the genus Pratylenchoides, including P. persicus n. sp. This new species is characterized by having lip region rounded or slightly flattened anteriorly with four or five fine but distinct annuli, pharyngeal glands off-set or slightly overlapping the intestine dorsally, all three gland nuclei located anterior to the pharyngo-intestinal valve and tail cylindrical with truncate to low rounded terminus. Morphologically, P. persicus n. sp. can be distinguished from the most closely related species, P. heathi by having shorter body and stylet length in females and males, as well as a shorter tail with different terminus in females. Pratylenchoides laticauda and P. cf. nevadensis are reported from Iran for the first time. Phylogenetic analysis based on the D2/D3 region of the large subunit of ribosomal DNA revealed Pratylenchoides as a monophyletic genus, and it supports the delineation of the new species, P. persicus n. sp. 

Nematodes from galls on Myrtaceae. II. Fergusobia/Fergusonina from small axillary bud ('stem') and leaf ('pea') galls in Australia, with descriptions of two new species

Zootaxa ◽  
2012 ◽  
Vol 3415 (1) ◽  
pp. 1 ◽  
Author(s):  
KERRIE DAVIES ◽  
ROBIN GIBLIN-DAVIS ◽  
WEIMIN YE ◽  
GARY TAYLOR ◽  
KELLEY THOMAS
Keyword(s):  
New Species ◽  
Large Subunit ◽  
South Australia ◽  
Eucalyptus Camaldulensis ◽  
Parthenogenetic Female ◽  
Unknown Species ◽  
North Eastern ◽  
Two New Species ◽  
Large Subunit Rdna ◽  
Tail Tip

Two new species of Fergusobia, collected from small multilocular axillary vegetative bud ('stem') and unilocular leaf 'pea' galls on, respectively, Eucalyptus camaldulensis from South Australia, and an unknown species of Corymbia growing in sub-coastal north-eastern NSW, Australia, are described. Fergusobia camaldulensae n. sp. Davies is characterized morphologically by an almost straight to open-C shaped parthenogenetic female with a broadly conoid tail, an arcuate infective female with a hemispherical tail tip, and males of varying shape with weakly sclerotised angular spicules and bursa arising at mid-body length. Fergusobia rileyi n. sp. Davies is characterized morphologically by the combination of an almost straight to arcuate parthenogenetic female with a broadly conoid tail, an arcuate infective female with relatively narrow tail with a pointed tip, and arcuate males with arcuate spicules, a relatively slender tail, and long peloderan bursa. These species are associated with larvae of undescribed fly species having dorsal shields of the 'transverse bars' form or lacking. Other apparently related forms of Fergusobia/Fergusonina associations from small 'stem' and 'shoot' galls and unilocular leaf 'pea' galls are reported, the larval shield morphology of their associated mutualistic fly species is discussed where known, and their possible relationships are outlined. DNA sequence analyses of the domain 2 and 3 segments of the large subunit rDNA gene (D2/D3) and mitochondrial DNA cytochrome oxidase subunit 1 (mtCOI) supported the new species status and their phylogenetic relationships with other sequenced species in Fergusobia.

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