Pars flaccida perforation in tissue culture: morphological study

1990 ◽  
Vol 104 (2) ◽  
pp. 91-96 ◽  
Author(s):  
L.-E. Stenfors ◽  
E. G. Olsen ◽  
A. Ö. Henriksen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Tissue Culture ◽  
Inflammatory Reaction ◽  
Granulation Tissue ◽  
Contact Area ◽  
Morphological Study ◽  
Squamous Epithelium ◽  
Pars Flaccida ◽  
Scanning Electron

AbstractSubtotal pars flaccida perforation was effected in vitro in rat tympanic membranes. The drums were subsequently kept in tissue culture and after various intervals studied by means of light and scanning electron microscopy. No complete covering of the perforation was seen, though a marked thickening and hyperplasia of the outer, keratinizing, squamous epithelium (OE) was evident. The inner, tympanal epithelium (IE) appeared swollen, containing rounded structures in the cytoplasm especially close to the contact area with the OE. Ingrowth of OE onto the tympanal side of the drum was seen particularly in the areas where the IE was sparse and completely lacking. Complete covering of a drum perforation seems to be dependent on the formation of supporting granulation tissue, probably resulting from an inflammatory reaction in the healing area.

Surface structures of aural polyps: a scanning electron microscopic study

2002 ◽  
Vol 116 (6) ◽  
pp. 420-425 ◽  
Author(s):  
J. Skladzień ◽  
J. A. Litwin ◽  
M. Nowogrodzka-Zagórska ◽  
A. J. Miodoński
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Granulation Tissue ◽  
Squamous Epithelium ◽  
Surface Structures ◽  
Epithelial Lining ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Surfaces of aural polyps collected from 30 patients were examined by scanning electron microscopy. In the polyps not associated with cholesteatoma, the epithelial lining showed individually variable metaplasia towards cuboidal ‘cobblestone’-type and squamous epithelium covered with microvilli of various shapes and sizes. Squamous epithelium was present on the surface of all polyps with underlying cholesteatoma, with superficial cells possessing elongated microvilli, microplicae of different sizes, grooves and pits. Such surface structures reflect different stages of the keratinization process that seems to becharacteristic for the epithelial lining of polyps with underlying cholesteatoma. Incomplete epithelium accompanied by granulation tissue was found in several polyps; in two cholesteatoma-associated polyps plate-likecholesterol crystals were observed.

Study of the nasopharynx in man by scanning electron microscopy

1988 ◽  
Vol 102 (12) ◽  
pp. 1102-1106 ◽  
Author(s):  
M. Gulisano ◽  
G. Polli ◽  
G. Biondi ◽  
P. Pacini
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Healthy Volunteers ◽  
Squamous Epithelium ◽  
Goblet Cells ◽  
Transitional Epithelium ◽  
Ciliated Cells ◽  
Scanning Electron

AbstractTo date only few and often disagreeing studies about human nasopharynx are available.The present research has the purpose to give a contribution to the knowledge of nasopharyngeal epithelium using S.E.M The study was carried out on biopsies taken from 20 healthy volunteers.The surface of nasopharynx is covered by ciliated cells, microvilli provided cells and goblet cells.Areas covered with squamous epithelium and presumably transitional epithelium were observed.The possibility that ciliated become microvilli provided cells is discussed.

scholarly journals The mechanism of somite segmentation in the chick embryo

Development ◽  
1979 ◽  
Vol 51 (1) ◽  
pp. 227-243
Author(s):  
Ruth Bellairs
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Tissue Culture ◽  
Chick Embryo ◽  
Neural Tube ◽  
Cell Shape ◽  
Fibroblast Cell ◽  
General Shape ◽  
Major Factors ◽  
Scanning Electron

The segmentation of somites in the chick embryo has been studied by transmission and scanning electron microscopy (stages 8–14). The segmental plate mesoderm consists of loosely arranged mesenchymal cells, whereas the newly formed somites are composed of elongated, spindle-shaped cells arranged radially around a lumen, the myocoele. The diameter of each somite is thus two cells plus the myocoele. Two major factors appear to be responsible for the change in cell shape at segmentation: (1) Each prospective somite cell becomes anchored at one end to the adjacent epithelia (i.e. the neural tube, the notochord, the ectoderm, the endoderm or the aorta) by means of collagen fibrils. These fibrils are already present in the segmental plate before the somites begin to form. (2) A change in cell-to-cell adhesiveness causes the free ends of these cells to adhere to one another. (Bellairs, Curtis & Sanders, 1978). This adhesion is then supplemented by the development of tight junctions proximally in the somite. Because it is anchored at both ends, each somite cell is under tension in much the same way as a fibroblast cell in tissue culture is under tension. Each somite cell therefore becomes elongated and the somite as a whole accommodates its general shape to that of the space available between the adjacent tissues. The arrangement of the cells in the more differentiated somites (stages 17–18) has also been examined and it has been found that the chick resembles Xenopus in that the myotome cells undergo rotation and become orientated in an anteroposterior direction.

Sclerosing Peritonitis -The Contribution of Chlorhexidine in Alcohol

1985 ◽  
Vol 5 (2) ◽  
pp. 101-104 ◽  
Author(s):  
Brian J. R. Junor ◽  
J. Douglas Briggs ◽  
Margaret A. Forwell ◽  
James W. Dobbie ◽  
Iain Henderson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Peritoneal Dialysis ◽  
Peritoneal Cavity ◽  
Inflammatory Reaction ◽  
Fold Increase ◽  
Sclerosing Peritonitis ◽  
Particle Count ◽  
Fibrotic Process ◽  
Scanning Electron

Sclerosing peritonitis now is recognized as an extremely serious complication of peritoneal dialysis. In Glasgow II of 162 patients on CAPD developed sclerosing peritonitis. All 11 came from a group of 54 patients, who used a connecting system that allowed small quantities of the antiseptic spray, chlorhexidine in alcohol, to enter the abdomen. Experiments showed that I ml of this solution added to 2 litres of dialysate produced up to a 20. fold increase in the particle count of the dialysate. Scanning electron microscopy suggested that these particles probably were plastic. In rats intraperitoneal injections of a mixture of antiseptic spray and dialysate produced an inflammatory reaction in the submesothelial tissues, which might represent the early changes of a fibrotic process. To prevent possible longterm complications such as sclerosing peritonitis we should avoid the introduction into the peritoneal cavity of any unnecessary substance and in particular chlorhexidine in alcohol.

Sign in / Sign up

Export Citation Format

Share Document