Expression and clinical significance of Ki-67, oestrogen and progesterone receptors in acoustic neuroma

2007 ◽  
Vol 122 (2) ◽  
pp. 125-127 ◽  
Author(s):  
S Cafer ◽  
I Bayramoglu ◽  
N Uzum ◽  
M Yilmaz ◽  
L Memis ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Acoustic Neuroma ◽  
Oestrogen Receptor ◽  
Hormone Receptors ◽  
Tumour Size ◽  
Progesterone Receptors ◽  
Inhibitory Effect ◽  
Receptor Expression ◽  
Ki 67 ◽  
Tissue Samples

AbstractObjective:The objective was to assess the presence of Ki-67, and oestrogen and progesterone hormone receptors as well as their clinical correlates in acoustic neuroma.Methods:Medical records of 59 patients who were operated on for acoustic neuroma between 1995 and 2003 were evaluated retrospectively. Formaldehyde-fixed paraffin-embedded archival acoustic neuroma specimens of the patients were used for immunohistochemical assessments of oestrogen and progesterone hormone receptors, and Ki-67 proliferative marker.Results:Tumour sizes were small (<19 mm), medium (20–39 mm) and large (>40 mm) in 21, 35 and 3 patients, respectively. On immunohistochemistry, all samples were (+) for progesterone receptor and (–) for oestrogen receptor staining. Ki-67 staining was encountered in 34 of 59 (57.6 per cent) patients, and Ki-67 values ranged from 0 per cent to 10.9 per cent (mean 1.36 per cent). There was no correlation between Ki-67, gender, tumour size and symptoms of the patients (p > 0.05).Conclusion:Oestrogen is not an important hormone in acoustic neuroma due to the absence of oestrogen receptor expression in the tissue samples. Since the progesterone receptor is expressed in all acoustic neuroma samples, further studies are necessary to find out about the inhibitory effect of antiprogesterone treatment on acoustic neuroma growth, which may be important particularly in elderly people or high-risk patients. Although Ki-67 is expressed in the majority of acoustic neuromas, it is not an important marker in clinical practice due to a lack of any correlation with the clinical parameters.

scholarly journals Oestrogen Receptor, Progesterone Receptor Expression on Epithelial Tumours of Ovary and Correlation with Their Clinicopathological Features

2021 ◽  
Vol 10 (42) ◽  
pp. 3645-3648
Author(s):  
Soumya Jose ◽  
Seena A.R.
Keyword(s):  
Progesterone Receptor ◽  
Oestrogen Receptor ◽  
Hormone Receptors ◽  
Transitional Cell ◽  
Receptor Expression ◽  
Tissue Samples ◽  
Female Sex Hormones ◽  
Mucinous Tumours ◽  
Epithelial Tumours ◽  
The Many

BACKGROUND Ovarian carcinoma is one of the most lethal malignancies. Their histogenesis and complex pathogenesis remain largely unknown in spite of the many studies and research carried out in the field. The receptors for female sex hormones are implicated in the pathogenesis of ovarian tumours in many studies. This concept points out the necessity of developing a highly affected targeted therapy, which requires a proper understanding of the pathogenesis of the tumours. This study was done to evaluate the expression of these receptors on the primary epithelial tumours of the ovary and explore the possible correlation with clinical and pathological features. METHODS A hundred cases of primary epithelial tumours of the ovary were selected; tissue samples were taken from appropriate areas and processed. Tissues were cut into sections of three to five-micron thickness. Sections from the tissues were stained and examined. Once the histological type was clear, the receptor expression was assessed with immunohistochemistry markers. RESULTS Among the hundred tumours studied, serous tumours were the commonest, accounting for 65 % followed by mucinous tumours which constituted 34 %. Clear cell tumours accounted for 1 %. Endometrioid and transitional cell tumours were still rarer. Among these, oestrogen receptor (ER) was expressed in 78.5 % of serous tumours and progesterone receptor (PR) was expressed in 64.6 % of serous tumours. CONCLUSIONS Serous tumours were seen to show maximum expression of the hormone receptors among the surface tumours of ovaries. Furthermore, the expression of the receptors was more consistently seen in high-grade tumours. This finding may be of help in designing personalized hormone therapy in epithelial tumours. KEY WORDS Surface Epithelial Tumours, Receptors, ER, PR.

scholarly journals Insufficient Angiogenesis: Cause of Abnormally Thin Endometrium in Subfertile Patients?

2017 ◽  
Vol 77 (07) ◽  
pp. 756-764 ◽  
Author(s):  
Joachim Alfer ◽  
Lars Happel ◽  
Ralf Dittrich ◽  
Matthias Beckmann ◽  
Arndt Hartmann ◽  
...  
Keyword(s):  
Hormone Receptors ◽  
Steroid Hormone ◽  
Receptor Activation ◽  
Receptor Expression ◽  
Steroid Hormone Receptors ◽  
Uterine Disease ◽  
Secretory Phase ◽  
Ki 67 ◽  
Tissue Samples ◽  
Thin Endometrium

Abstract Introduction This study investigated subfertile patients with abnormally thin endometrium after infertility treatment. As they had adequate serum concentrations of hormones, an endometrial factor for subfertility was suspected. Methods To elucidate the cause of subfertility, endometrial biopsies were taken in each patient in the late proliferative and mid-secretory phases of one menstrual cycle. Endometrial biopsies from women with normal menstrual cycles and confirmed fertility who were undergoing hysterectomy for benign uterine disease were used as positive controls. The tissue samples were investigated for steroid hormone receptor expression and for the proliferation marker Ki-67. Immunohistochemistry was performed with antibodies against the marker molecules for endometrial receptivity – β3 integrin, VEGF, LIF, and CD56 (large granular lymphocytes, LGLs). Results The steroid hormone receptors for estrogen (E2) and progesterone (P) were expressed normally (at the first biopsy) and were down-regulated (at the second biopsy) within the cycle. Strikingly, all of the marker molecules investigated showed negative or weak and inadequate expression in the mid-secretory phase. Numbers of LGLs remained as low as in the proliferative phase. In contrast, fertile patients were found to express these marker molecules distinctly in the mid-secretory phase. Conclusions It may be hypothesized that a severe deficiency of these angiogenesis-related marker molecules leads to defective development of the endometrium, which remains thin. Deficient angiogenetic development may thus provide an explanation for the endometrial factor that causes infertility. Further investigations will need to focus on identifying the regulating factors that act between steroid receptor activation and the expression of these marker molecules.

Oestrogen and progesterone receptors in acoustic neuroma

1990 ◽  
Vol 104 (11) ◽  
pp. 865-867 ◽  
Author(s):  
J. W. A. Curley ◽  
R. T. Ramsden ◽  
A. Howell ◽  
K. Healy ◽  
R. H. Lye
Keyword(s):  
Monoclonal Antibody ◽  
Isoelectric Focusing ◽  
Acoustic Neuroma ◽  
Hormone Receptors ◽  
Progesterone Receptors ◽  
Tissue Samples ◽  
Acoustic Neuromas ◽  
Dextran Coated Charcoal ◽  
Positive Results

AbstractTissue samples from fourteen consecutive (8 male: 6 female) acoustic neuromas were assayed for hormone receptors using either a monoclonal antibody (MA), dextran coated charcoal (DCC) or isoelectric focusing (IEF) technique.In this series there were no unequivocally positive results, a finding at variance with previously published results.

scholarly journals Interaction of oestrogen and progesterone receptors with specific subfractions of laying-hen oviduct chromatin

1984 ◽  
Vol 217 (1) ◽  
pp. 309-316 ◽  
Author(s):  
N Massol ◽  
M C Lebeau ◽  
E E Baulieu
Keyword(s):  
Progesterone Receptor ◽  
Oestrogen Receptor ◽  
Hormone Receptors ◽  
Steroid Hormone ◽  
Progesterone Receptors ◽  
Steroid Hormone Receptors ◽  
Target Tissue ◽  
Low Salt ◽  
High Concentrations

Salt (NaCl)-extracted nuclear oestrogen receptor from hen oviduct was incubated with salt-depleted oviduct chromatin and dialysed to low salt. The oestrogen receptor (re)associated with chromatin to form a 13-14S-sedimenting fraction, as found in ‘native’ chromatin, and saturation of this interaction was obtained for very low receptor concentrations (approx. 0.04 nM). Similarly, purified progesterone receptor from chick oviduct cytosol associated with depleted chromatin to form an 11-12S-sedimenting fraction, as in ‘native’ chromatin; this interaction tended towards saturation for much higher concentrations of progesterone receptor (approx. 8 nM) than that observed for oestrogen receptor. When the two receptors were incubated with depleted chromatin from hen kidney or erythrocytes, their s values were as for oviduct chromatin. However, no saturation of these interactions was seen, even for high concentrations of receptor. Steroid-hormone receptors can therefore bind in vitro to particular subfractions of non-target-tissue chromatin, but with a much lower affinity than to target-tissue chromatin.

scholarly journals Methamphetamine Administration Increases Proceptive Behavior by Activating Src Kinase and Upregulating Progesterone Receptor in the Medial Amygdala

2018 ◽  
Author(s):  
Katrina M Williams ◽  
Sarah A Rudzinskas ◽  
Jessica A Mong
Keyword(s):  
Progesterone Receptor ◽  
Phosphorylation Site ◽  
Progesterone Receptors ◽  
Receptor Expression ◽  
Medial Amygdala ◽  
Specific Manner ◽  
Receptor Action ◽  
Necessary And Sufficient ◽  
Motivated Behaviors

Methamphetamine, a psychostimulant drug of abuse, increases sexual motivation both in humans and in rodent models. The activation of dopamine type-1 receptors (D1Rs) within the medial amygdala, in the presence of ovarian hormones (EB+P), are both necessary and sufficient for increases in proceptive, or sexually motivated, behaviors. Here, we demonstrate that methamphetamine increases progesterone receptor expression in the medial amygdala independently of D1R activation, and that lentiviral overexpression of the progesterone receptor was able to recapitulate the methamphetamine-induced enhancement of proceptive behaviors. Furthermore, we found that within the medial amygdala, these progesterone receptors show an increase in phosphorylation of serine 294 of the progesterone receptor in a region-specific manner. The involvement of this phosphorylation site suggests a role for cytosolic kinases, which may be responsible for enhanced progesterone receptor action. The phosphorylation of serine 294 is blocked by D1R antagonists, and by inhibiting cSrc and ERK1/2, downstream of D1R signaling, we identified that Src and ERK1/2 are required for enhanced proceptive behavior. Taken together, we propose that within the medial amygdala, methamphetamine enhances progesterone receptors sensitivity to its cognate ligand via interaction with cSrc kinase and ERK1/2, as well as an increase total progesterone receptors, thus leading to enhanced proceptive behaviors in the rat.

scholarly journals Ki-67 Expression is a Significant Prognostic Factor Only When Progesterone Receptor Expression is Low in Estrogen Receptor-Positive and HER2-Negative Early Breast Cancer

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Young-Joon Kang ◽  
Han-Byoel Lee ◽  
Yun Gyoung Kim ◽  
JaiHong Han ◽  
Yumi Kim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Prognostic Factor ◽  
Progesterone Receptor ◽  
Cancer Patients ◽  
Early Breast Cancer ◽  
Receptor Expression ◽  
Breast Cancer Patients ◽  
Ki 67 ◽  
End Point

Objective. While the value of Ki-67 has been recognized in breast cancer, controversy also exists. The goal of this study is to show the prognostic value of Ki-67 according to progesterone receptor (PgR) expression in patients who have estrogen receptor- (ER-) positive, human epidermal growth factor receptor 2- (HER2-) negative early breast cancer. Methods. The records of nonmetastatic invasive breast cancer patients who underwent surgery at a single institution between 2009 and 2012 were reviewed. Primary end point was recurrence-free survival (RFS), and secondary end point was overall survival (OS). Ki-67 and PgR were assessed with immunohistochemistry for the tumor after surgery. Results. A total of 1848 patients were enrolled in this study. 223 (12%) patients had high (≥10%) Ki-67, and 1625 (88%) had low Ki-67 expression. Significantly worse RFS and OS were observed in the high vs. low Ki-67 expression only when the PgR was low (<20%) (p<0.001 and 0.005, respectively, for RFS and OS). There was no significant difference in RFS and OS according to Ki-67 when the PgR was high (p=0.120 and 0.076). RFS of four groups according to high/low Ki-67 and PgR expression was compared. The low PgR and high Ki-67 expression group showed worst outcome among them (p<0.001). In a multivariate analysis, high Ki-67 was an independent prognostic factor when the PgR was low (HR 3.05; 95% CI 1.50–6.19; p=0.002). Conclusions. Ki-67 had a value as a prognostic factor only under low PgR expression level in early breast cancer. PgR should be considered in evaluating the prognosis of breast cancer patients using Ki-67.

Effect of nutrition on endometrial progesterone receptor expression in ewes

2003 ◽  
Vol 2003 ◽  
pp. 81-81
Author(s):  
J.A. Abecia ◽  
C. Sosa ◽  
J.M. Lozano ◽  
C. Viñoles ◽  
F. Forcada ◽  
...  
Keyword(s):  
Food Intake ◽  
Progesterone Receptor ◽  
Progesterone Receptors ◽  
Cell Types ◽  
Receptor Expression ◽  
Progesterone Receptor Expression ◽  
Endometrial Cell ◽  
Embryo Survival ◽  
Plasma Progesterone ◽  
Uterine Environment

It has been postulated that undernourishment could affect embryo survival through changes in the uterine environment (Abecia et al., 1995). Moreover, we have shown that undernourished ewes had higher plasma progesterone (P4) concentrations and a lower endometrial content of P4 (Lozano et al., 1998), suggesting that this lower endometrial content could be due to a decrease in the content of endometrial progesterone receptors (PR). The aim of this study was to investigate the effect of low and high levels of food intake on PR in different endometrial cell types.

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