scholarly journals Oestrogen Receptor, Progesterone Receptor Expression on Epithelial Tumours of Ovary and Correlation with Their Clinicopathological Features

2021 ◽  
Vol 10 (42) ◽  
pp. 3645-3648
Author(s):  
Soumya Jose ◽  
Seena A.R.
Keyword(s):  
Progesterone Receptor ◽  
Oestrogen Receptor ◽  
Hormone Receptors ◽  
Transitional Cell ◽  
Receptor Expression ◽  
Tissue Samples ◽  
Female Sex Hormones ◽  
Mucinous Tumours ◽  
Epithelial Tumours ◽  
The Many

BACKGROUND Ovarian carcinoma is one of the most lethal malignancies. Their histogenesis and complex pathogenesis remain largely unknown in spite of the many studies and research carried out in the field. The receptors for female sex hormones are implicated in the pathogenesis of ovarian tumours in many studies. This concept points out the necessity of developing a highly affected targeted therapy, which requires a proper understanding of the pathogenesis of the tumours. This study was done to evaluate the expression of these receptors on the primary epithelial tumours of the ovary and explore the possible correlation with clinical and pathological features. METHODS A hundred cases of primary epithelial tumours of the ovary were selected; tissue samples were taken from appropriate areas and processed. Tissues were cut into sections of three to five-micron thickness. Sections from the tissues were stained and examined. Once the histological type was clear, the receptor expression was assessed with immunohistochemistry markers. RESULTS Among the hundred tumours studied, serous tumours were the commonest, accounting for 65 % followed by mucinous tumours which constituted 34 %. Clear cell tumours accounted for 1 %. Endometrioid and transitional cell tumours were still rarer. Among these, oestrogen receptor (ER) was expressed in 78.5 % of serous tumours and progesterone receptor (PR) was expressed in 64.6 % of serous tumours. CONCLUSIONS Serous tumours were seen to show maximum expression of the hormone receptors among the surface tumours of ovaries. Furthermore, the expression of the receptors was more consistently seen in high-grade tumours. This finding may be of help in designing personalized hormone therapy in epithelial tumours. KEY WORDS Surface Epithelial Tumours, Receptors, ER, PR.

Expression and clinical significance of Ki-67, oestrogen and progesterone receptors in acoustic neuroma

2007 ◽  
Vol 122 (2) ◽  
pp. 125-127 ◽  
Author(s):  
S Cafer ◽  
I Bayramoglu ◽  
N Uzum ◽  
M Yilmaz ◽  
L Memis ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Acoustic Neuroma ◽  
Oestrogen Receptor ◽  
Hormone Receptors ◽  
Tumour Size ◽  
Progesterone Receptors ◽  
Inhibitory Effect ◽  
Receptor Expression ◽  
Ki 67 ◽  
Tissue Samples

AbstractObjective:The objective was to assess the presence of Ki-67, and oestrogen and progesterone hormone receptors as well as their clinical correlates in acoustic neuroma.Methods:Medical records of 59 patients who were operated on for acoustic neuroma between 1995 and 2003 were evaluated retrospectively. Formaldehyde-fixed paraffin-embedded archival acoustic neuroma specimens of the patients were used for immunohistochemical assessments of oestrogen and progesterone hormone receptors, and Ki-67 proliferative marker.Results:Tumour sizes were small (<19 mm), medium (20–39 mm) and large (>40 mm) in 21, 35 and 3 patients, respectively. On immunohistochemistry, all samples were (+) for progesterone receptor and (–) for oestrogen receptor staining. Ki-67 staining was encountered in 34 of 59 (57.6 per cent) patients, and Ki-67 values ranged from 0 per cent to 10.9 per cent (mean 1.36 per cent). There was no correlation between Ki-67, gender, tumour size and symptoms of the patients (p > 0.05).Conclusion:Oestrogen is not an important hormone in acoustic neuroma due to the absence of oestrogen receptor expression in the tissue samples. Since the progesterone receptor is expressed in all acoustic neuroma samples, further studies are necessary to find out about the inhibitory effect of antiprogesterone treatment on acoustic neuroma growth, which may be important particularly in elderly people or high-risk patients. Although Ki-67 is expressed in the majority of acoustic neuromas, it is not an important marker in clinical practice due to a lack of any correlation with the clinical parameters.

Intrauterine injection of ovine interferon-τ alters oestrogen receptor and oxytocin receptor expression in the endometrium of cyclic ewes

1995 ◽  
Vol 15 (2) ◽  
pp. 203-220 ◽  
Author(s):  
T E Spencer ◽  
N H Ing ◽  
T L Ott ◽  
J S Mayes ◽  
W C Becker ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Oestrogen Receptor ◽  
Plasma Concentrations ◽  
Oxytocin Receptor ◽  
Receptor Expression ◽  
Glandular Epithelium ◽  
Receptor Density ◽  
Receptor Mrna ◽  
Prostaglandin F

ABSTRACT This study determined the effects of intrauterine injections of recombinant ovine interferon-τ (roIFN-τ; 2 × 107 antiviral units/day) or control proteins (6 mg/day) from day 11 to day 14 post-oestrus (oestrus=day 0) on endometrial expression of receptors for oestrogen, progesterone and oxytocin in cyclic ewes. Plasma concentrations of progesterone were greater on day 15 in ewes receiving roIFN-τ compared with control proteins (P<0·02, treatment × day). Ewes injected with roIFN-τ had lower endometrial levels of oestrogen receptor mRNA (P<0·10) and protein (P<0·01) on day 15 compared with ewes receiving control proteins. In situ hybridization analysis indicated that oestrogen receptor mRNA was more abundant in the luminal and glandular epithelium of control ewes compared with roIFN-τ-treated ewes. Immunoreactive oestrogen receptor was also present in the luminal and glandular epithelium of control, but not roIFN-τ-treated ewes. Endometrial levels of progesterone receptor mRNA and protein were not different (P>0·10) between control and roIFN-τ-treated ewes. In situ hybridization analyses indicated that progesterone receptor mRNA abundance was low in endometrial epithelium and stroma of both control and roIFN-τ-injected ewes. Immunoreactive progesterone receptors were present in the endometrial stroma and epithelium of control ewes, but confined to the stroma of roIFN-τ-treated ewes. Oxytocin receptor density was lower (P<0·10) in the endometrium of ewes injected with roIFN-τ than control proteins; however, oxytocin receptor affinity was not affected (P>0·10) by treatment. Concentrations of 13,14-dihydro-15-keto-prostaglandin F2α (PGFM) were not increased by exogenous oxytocin administration in control and roIFN-τ-treated ewes on days 10 or 12 post-oestrus. However, on day 14, control ewes responded to oxytocin with increased plasma concentrations of PGFM, whereas ewes receiving roIFN-τ remained unresponsive to oxytocin. These results indicate that the antiluteolytic effects of IFN-τ are to prevent increases in endometrial oestrogen receptor mRNA and protein and oxytocin receptor density which abrogates uterine release of prostaglandin F2α during maternal recognition of pregnancy. IFN-τ may inhibit the synthesis of oestrogen receptor mRNA by a transcriptional or post-transcriptional regulatory mechanism to suppress oxytocin receptor formation during early pregnancy in ewes.

scholarly journals Insufficient Angiogenesis: Cause of Abnormally Thin Endometrium in Subfertile Patients?

2017 ◽  
Vol 77 (07) ◽  
pp. 756-764 ◽  
Author(s):  
Joachim Alfer ◽  
Lars Happel ◽  
Ralf Dittrich ◽  
Matthias Beckmann ◽  
Arndt Hartmann ◽  
...  
Keyword(s):  
Hormone Receptors ◽  
Steroid Hormone ◽  
Receptor Activation ◽  
Receptor Expression ◽  
Steroid Hormone Receptors ◽  
Uterine Disease ◽  
Secretory Phase ◽  
Ki 67 ◽  
Tissue Samples ◽  
Thin Endometrium

Abstract Introduction This study investigated subfertile patients with abnormally thin endometrium after infertility treatment. As they had adequate serum concentrations of hormones, an endometrial factor for subfertility was suspected. Methods To elucidate the cause of subfertility, endometrial biopsies were taken in each patient in the late proliferative and mid-secretory phases of one menstrual cycle. Endometrial biopsies from women with normal menstrual cycles and confirmed fertility who were undergoing hysterectomy for benign uterine disease were used as positive controls. The tissue samples were investigated for steroid hormone receptor expression and for the proliferation marker Ki-67. Immunohistochemistry was performed with antibodies against the marker molecules for endometrial receptivity – β3 integrin, VEGF, LIF, and CD56 (large granular lymphocytes, LGLs). Results The steroid hormone receptors for estrogen (E2) and progesterone (P) were expressed normally (at the first biopsy) and were down-regulated (at the second biopsy) within the cycle. Strikingly, all of the marker molecules investigated showed negative or weak and inadequate expression in the mid-secretory phase. Numbers of LGLs remained as low as in the proliferative phase. In contrast, fertile patients were found to express these marker molecules distinctly in the mid-secretory phase. Conclusions It may be hypothesized that a severe deficiency of these angiogenesis-related marker molecules leads to defective development of the endometrium, which remains thin. Deficient angiogenetic development may thus provide an explanation for the endometrial factor that causes infertility. Further investigations will need to focus on identifying the regulating factors that act between steroid receptor activation and the expression of these marker molecules.

scholarly journals HISTOPATHOLOGICAL PATTERNS OF SURFACE EPITHELIAL TUMOURS OF OVARY WITH OESTROGEN AND PROGESTERONE RECEPTOR EXPRESSION

2016 ◽  
Vol 5 (67) ◽  
pp. 4783-4789
Author(s):  
Arun Harke B ◽  
Ramamurthy Madhumittha ◽  
Sekar Manjani ◽  
Thukkaram Chitra ◽  
Ekambaranathan Saravanan ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Receptor Expression ◽  
Progesterone Receptor Expression ◽  
Epithelial Tumours

Evidence for oestrogenic regulation of heat shock protein expression in human endometrium and steroid-responsive cell lines

1995 ◽  
Vol 133 (5) ◽  
pp. 598-605 ◽  
Author(s):  
Pei-Zhong Tang ◽  
Michael J Gannon ◽  
Alison Andrew ◽  
David Miller
Keyword(s):  
Menstrual Cycle ◽  
Heat Shock ◽  
Progesterone Receptor ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Cell Lines ◽  
Oestrogen Receptor ◽  
Receptor Expression ◽  
Heat Shock Protein Expression ◽  
Responsive Cell

Tang P-Z, Gannon MJ, Andrew A, Miller D. Evidence for oestrogenic regulation of heat shock protein expression in human endometrium and steroid-responsive cell lines. Eur J Endocrinol 1995;133:598–605. ISSN 0804–4643 Gene amplification with target-specific primers (reverse-transcription polymerase chain reaction (RTPCR)) was used to monitor the relative expression of oestrogen and progesterone receptor mRNAs alongside the mRNAs for heat shock proteins HSP 90α, HSP 90β and HSP 70a in normal samples of human endometrial tissue over the whole menstrual cycle and in short-term cultures of steroidresponsive (T47-D) and unresponsive (HRT-18) cell lines exposed to oestradiol and progesterone over a 24-h incubation period. In endometrium, oestrogen and progesterone receptors followed the expected patterns of expression at the protein level during the menstrual cycle and also showed a positive correlation of expression with each other throughout (r = 0.514). Of the HSPs only HSP 90α expression correlated positively with oestrogen receptor (r = 0.687), while HSP 70a expression, which peaked in the late secretory stage, displayed a significantly inverse correlation with HSP 90β expression (r = −0.526). All p values < 0.05. In T47-D cell cultures, oestrogen receptor expression was stimulated transiently by oestradiol (10−7 mol/l) and more persistently by progesterone (10−7 mol/l). Progesterone receptor expression was depressed by progesterone and weakly stimulated by oestradiol. HSP 70a and HSP 90α expression were stimulated by oestradiol. Progesterone generally depressed HSP 90α expression and simultaneous addition of both oestradiol and progesterone to the culture medium was antagonistic to HSP 90α expression. No clear effect of agonist addition on HSP mRNA expression was apparent in the HRT-18 cultures. A possible mechanism for observed oestrogenic effects on HSP expression is put forward. David Miller, Institute of Pathology, Department of Clinical Medicine, Algernon Firth Building, University of Leeds, Leeds, LS2 9JT UK

scholarly journals Overall Survival in Advanced Breast Cancer: Relevance of Progesterone Receptor Expression and DNA Ploidy in Fine-Needle Aspirates of 392 Patients

2003 ◽  
Vol 18 (1) ◽  
pp. 7-12 ◽  
Author(s):  
A.E. Pinto ◽  
S. André ◽  
E. Mendonça ◽  
G. Silva ◽  
J. Soares
Keyword(s):  
Breast Cancer ◽  
Overall Survival ◽  
Progesterone Receptor ◽  
Advanced Breast Cancer ◽  
Hormone Receptors ◽  
Dna Ploidy ◽  
Receptor Expression ◽  
Advanced Breast ◽  
Disease Stage ◽  
Progesterone Receptor Expression

Fine-needle aspiration cytology (FNAC) is essential for making a diagnosis in advanced breast cancer. The determination of hormone receptors in the material obtained is useful for predicting patient response to endocrine therapy, but the prognostic value of hormone receptor expression as well as the clinical utility of DNA flow cytometry are controversial. The aim of this prospective study with long-term follow-up (median: 81 months) was to evaluate these biomarkers in relation to overall survival in a series of 392 patients with advanced breast cancer (stage IIB, n=106; IIIA, n=66; IIIB, n=174; and IV, n=46) using FNAC. Estrogen and progesterone receptor expression was found in 65.1% and 46.1% of the tumors, respectively. Hormone receptors were not found to be associated with clinical staging. DNA aneuploidy was present in 70.9% of the cases and the median S-phase fraction (SPF) was 9.4%. There was a significant correlation of aneuploidy and high SPF with lack of hormone receptors. In univariate analysis, advanced disease stage, absence of hormone receptors, DNA aneuploidy and high SPF showed a statistically significant correlation with poor clinical outcome. In multivariate analysis, disease stage, progesterone receptors and DNA ploidy retained independent prognostic significance in relation to overall survival. These data indicate that progesterone receptor expression and DNA ploidy are independent prognostic factors in advanced breast cancer.

scholarly journals Interaction of oestrogen and progesterone receptors with specific subfractions of laying-hen oviduct chromatin

1984 ◽  
Vol 217 (1) ◽  
pp. 309-316 ◽  
Author(s):  
N Massol ◽  
M C Lebeau ◽  
E E Baulieu
Keyword(s):  
Progesterone Receptor ◽  
Oestrogen Receptor ◽  
Hormone Receptors ◽  
Steroid Hormone ◽  
Progesterone Receptors ◽  
Steroid Hormone Receptors ◽  
Target Tissue ◽  
Low Salt ◽  
High Concentrations

Salt (NaCl)-extracted nuclear oestrogen receptor from hen oviduct was incubated with salt-depleted oviduct chromatin and dialysed to low salt. The oestrogen receptor (re)associated with chromatin to form a 13-14S-sedimenting fraction, as found in ‘native’ chromatin, and saturation of this interaction was obtained for very low receptor concentrations (approx. 0.04 nM). Similarly, purified progesterone receptor from chick oviduct cytosol associated with depleted chromatin to form an 11-12S-sedimenting fraction, as in ‘native’ chromatin; this interaction tended towards saturation for much higher concentrations of progesterone receptor (approx. 8 nM) than that observed for oestrogen receptor. When the two receptors were incubated with depleted chromatin from hen kidney or erythrocytes, their s values were as for oviduct chromatin. However, no saturation of these interactions was seen, even for high concentrations of receptor. Steroid-hormone receptors can therefore bind in vitro to particular subfractions of non-target-tissue chromatin, but with a much lower affinity than to target-tissue chromatin.

scholarly journals Preliminary investigation of the influence of long-term dietary isoflavone intake on reproductive tract histology and sex steroid receptor expression in female domestic cats (Felis catus)

2012 ◽  
Vol 1 ◽  
Author(s):  
Katherine M. Whitehouse-Tedd ◽  
Nicholas J. Cave ◽  
Wendi D. Roe ◽  
Claudia E. Ugarte ◽  
David G. Thomas
Keyword(s):  
Oestrogen Receptor ◽  
Hormone Receptors ◽  
Reproductive Tract ◽  
Mammalian Species ◽  
Steroid Receptor ◽  
Receptor Expression ◽  
Sex Steroid ◽  
Domestic Cats ◽  
Reproductive Tissues ◽  
Steroid Receptor Expression

SummaryGenistein and daidzein are isoflavones which are reported to influence the reproductive system in a variety of mammalian species. This pilot study aimed to determine if dietary isoflavones could potentially influence reproductive tract histology or morphology in domestic cats, when consumed during the postnatal development period. Cats were maintained on either treatment (150 µg/g DM genistein and 150 µg/g DM daidzein, n = 4) or control (isoflavone free, n = 8) diets from weaning, up to 414 (±17.2) days post-weaning. Reproductive tissues were collected during routine ovario-hysterectomy and examined for histology and sex steroid receptor expression. Findings indicate that these dietary isoflavones influenced the expression of oestrogen receptor α (ERα) and oestrogen receptor β (ERβ), and progesterone receptor in feline reproductive tissues. One cat in the treatment group developed suppurative endometritis, but no evidence of uterotrophic or histological changes were found in any other cats. The potential to alter expression of hormone receptors in the reproductive tract of domestic cats exposed to genistein and daidzein warrants further investigation.

Sign in / Sign up

Export Citation Format

Share Document