scholarly journals Methamphetamine Administration Increases Proceptive Behavior by Activating Src Kinase and Upregulating Progesterone Receptor in the Medial Amygdala

2018 ◽  
Author(s):  
Katrina M Williams ◽  
Sarah A Rudzinskas ◽  
Jessica A Mong
Keyword(s):  
Progesterone Receptor ◽  
Phosphorylation Site ◽  
Progesterone Receptors ◽  
Receptor Expression ◽  
Medial Amygdala ◽  
Specific Manner ◽  
Receptor Action ◽  
Necessary And Sufficient ◽  
Motivated Behaviors

Methamphetamine, a psychostimulant drug of abuse, increases sexual motivation both in humans and in rodent models. The activation of dopamine type-1 receptors (D1Rs) within the medial amygdala, in the presence of ovarian hormones (EB+P), are both necessary and sufficient for increases in proceptive, or sexually motivated, behaviors. Here, we demonstrate that methamphetamine increases progesterone receptor expression in the medial amygdala independently of D1R activation, and that lentiviral overexpression of the progesterone receptor was able to recapitulate the methamphetamine-induced enhancement of proceptive behaviors. Furthermore, we found that within the medial amygdala, these progesterone receptors show an increase in phosphorylation of serine 294 of the progesterone receptor in a region-specific manner. The involvement of this phosphorylation site suggests a role for cytosolic kinases, which may be responsible for enhanced progesterone receptor action. The phosphorylation of serine 294 is blocked by D1R antagonists, and by inhibiting cSrc and ERK1/2, downstream of D1R signaling, we identified that Src and ERK1/2 are required for enhanced proceptive behavior. Taken together, we propose that within the medial amygdala, methamphetamine enhances progesterone receptors sensitivity to its cognate ligand via interaction with cSrc kinase and ERK1/2, as well as an increase total progesterone receptors, thus leading to enhanced proceptive behaviors in the rat.

Effect of nutrition on endometrial progesterone receptor expression in ewes

2003 ◽  
Vol 2003 ◽  
pp. 81-81
Author(s):  
J.A. Abecia ◽  
C. Sosa ◽  
J.M. Lozano ◽  
C. Viñoles ◽  
F. Forcada ◽  
...  
Keyword(s):  
Food Intake ◽  
Progesterone Receptor ◽  
Progesterone Receptors ◽  
Cell Types ◽  
Receptor Expression ◽  
Progesterone Receptor Expression ◽  
Endometrial Cell ◽  
Embryo Survival ◽  
Plasma Progesterone ◽  
Uterine Environment

It has been postulated that undernourishment could affect embryo survival through changes in the uterine environment (Abecia et al., 1995). Moreover, we have shown that undernourished ewes had higher plasma progesterone (P4) concentrations and a lower endometrial content of P4 (Lozano et al., 1998), suggesting that this lower endometrial content could be due to a decrease in the content of endometrial progesterone receptors (PR). The aim of this study was to investigate the effect of low and high levels of food intake on PR in different endometrial cell types.

Gonadal steroids modulated hypocretin/orexin type-1 receptor expression in a brain region, sex and daytime specific manner

2009 ◽  
Vol 158 (1-3) ◽  
pp. 121-126 ◽  
Author(s):  
Patricia Silveyra ◽  
Natalia I. Cataldi ◽  
Victoria Lux-Lantos ◽  
Carlos Libertun
Keyword(s):  
Brain Region ◽  
Receptor Expression ◽  
Gonadal Steroids ◽  
Specific Manner

Expression and clinical significance of Ki-67, oestrogen and progesterone receptors in acoustic neuroma

2007 ◽  
Vol 122 (2) ◽  
pp. 125-127 ◽  
Author(s):  
S Cafer ◽  
I Bayramoglu ◽  
N Uzum ◽  
M Yilmaz ◽  
L Memis ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Acoustic Neuroma ◽  
Oestrogen Receptor ◽  
Hormone Receptors ◽  
Tumour Size ◽  
Progesterone Receptors ◽  
Inhibitory Effect ◽  
Receptor Expression ◽  
Ki 67 ◽  
Tissue Samples

AbstractObjective:The objective was to assess the presence of Ki-67, and oestrogen and progesterone hormone receptors as well as their clinical correlates in acoustic neuroma.Methods:Medical records of 59 patients who were operated on for acoustic neuroma between 1995 and 2003 were evaluated retrospectively. Formaldehyde-fixed paraffin-embedded archival acoustic neuroma specimens of the patients were used for immunohistochemical assessments of oestrogen and progesterone hormone receptors, and Ki-67 proliferative marker.Results:Tumour sizes were small (<19 mm), medium (20–39 mm) and large (>40 mm) in 21, 35 and 3 patients, respectively. On immunohistochemistry, all samples were (+) for progesterone receptor and (–) for oestrogen receptor staining. Ki-67 staining was encountered in 34 of 59 (57.6 per cent) patients, and Ki-67 values ranged from 0 per cent to 10.9 per cent (mean 1.36 per cent). There was no correlation between Ki-67, gender, tumour size and symptoms of the patients (p > 0.05).Conclusion:Oestrogen is not an important hormone in acoustic neuroma due to the absence of oestrogen receptor expression in the tissue samples. Since the progesterone receptor is expressed in all acoustic neuroma samples, further studies are necessary to find out about the inhibitory effect of antiprogesterone treatment on acoustic neuroma growth, which may be important particularly in elderly people or high-risk patients. Although Ki-67 is expressed in the majority of acoustic neuromas, it is not an important marker in clinical practice due to a lack of any correlation with the clinical parameters.

scholarly journals AB0084 TNF RECEPTORS PROFILE CHANGES ON CYTOTOXIC T CELLS SUBSETS IN RHEUMATOID ARTHRITIS ARE ASSOCIATED WITH EFFECTIVE TREATMENT

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1341.3-1342
Author(s):  
A. Alshevskaya ◽  
J. Lopatnikova ◽  
J. Zhukova ◽  
F. Kireev ◽  
O. Chumasova ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
Effective Treatment ◽  
Expression Profile ◽  
Cytotoxic T Cells ◽  
Activity Level ◽  
Receptor Expression ◽  
Healthy Donors ◽  
Therapy Effect

Background:Previous studies of co-expression profile of receptors to tumor necrosis factor alpha (TNF) in rheumatoid arthritis (RA) have revealed a number of indicators associated with diseases activity with 93% sensitivity and 90% specificity. However, the ratio of receptors to cytokines remains poorly understood. However, the question of therapy effect and its effectiveness in various alteration of cytokine receptors balance remains under investigated.Objectives:To evaluate the dynamics of co-expression and quantitative expression of type 1 and 2 receptors for TNF in the subpopulations of CD3+CD8+ cells associated with changes in disease severity before and after effective basic therapy.Methods:Subanalysis of patients with high disease activity level successfully treated with methotrexate and oral glucocorticoids (n = 9) was performed. As a control group, we used data from 43 healthy donors, comparable by sex and age distribution. Subpopulations of cytotoxic T cells were studied, which were included in the final diagnostic models for differentiating different degrees of severity of RA: naive T cells and memory T cells. The dynamics of changes in the indicators of receptors number and proportion of cells expressing the corresponding receptor were compared.Results:For naïve cytotoxic T cells, the main revealed feature was the relative stability of the number of expressed receptors (both TNFR1 and TNFR2), regardless of the therapy, while this number did not significantly differ from healthy ones for TNFR1 and was significantly lower for TNFR2 (p <0.05 for all three fractions). At the same time, in terms of cell percentage, on the contrary, the therapy led to a change in total proportion of TNFR1 + cells closer to healthy donors indicators, and the proportion of TNFR2 + cells in the opposite direction.For cytotoxic T memory cells, it was demonstrated that after successful treatment a significant increase in the number of type 1 receptors was observed, with a decrease in TNFR1+ cells proportion, while these indicators were close to the values of healthy donors. At the same time, healthy donors were characterized by a significantly higher expression of type 2 receptors in terms of cell density of receptors. It is noteworthy that with successful therapy, a slight increase in the number of TNFR2 was observed with a sharp decrease in the proportion of TNFR2+ cells (p = 0.043).Conclusion:The balance of TNF receptor expression on cells actively involved in immunopathological processes affects both the density distribution of receptors on cells and co-expression in a subpopulation. Effective treatment of RA leads to equalization of the expression profile either by the percentage of cells or by the number of receptors, approaching the indicators of healthy donors, but not simultaneously.Disclosure of Interests:None declared

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