scholarly journals The Effect of Narcotics on the Endogenous Respiration and Succinate Oxidation in Oyster Muscle

1948 ◽  
Vol 27 (2) ◽  
pp. 504-512
Author(s):  
George Frederick Humphrey
Keyword(s):  
Salicylic Acid ◽  
Oxygen Consumption ◽  
Benzoic Acid ◽  
Succinic Acid ◽  
Chloral Hydrate ◽  
Endogenous Respiration ◽  
Succinate Oxidation ◽  
Adductor Muscles

The action of the following compounds on homogenates of the adductor muscles of Saxostrea commercialis was studied: urethane, phenyl urethane, chloral hydrate, salicylic acid, morphine, caffeine, barbitone, benzoic acid, salicylamide and benzamide.It was found that, except for 0·05 M urethane, the endogenous oxygen consumption was reduced by all these compounds when present in concentrations ranging from o·001 to 0·05M. The oxidation of succinic acid was partially inhibited by these substances.These findings are discussed in relation to similar studies on other tissues.

scholarly journals Alteration of Pseudomonas aeruginosa respiration by 4-(1-adamantyl)-phenol derivative

Biologija ◽  
2018 ◽  
Vol 64 (3) ◽  
Author(s):  
Daria M. Dudikova ◽  
Nina O. Vrynchanu ◽  
Valentyna I. Nosar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Oxygen Consumption ◽  
Oxidative Phosphorylation ◽  
Respiratory Chain ◽  
Structural Integrity ◽  
Respiratory Control ◽  
Endogenous Respiration ◽  
Phenol Derivative ◽  
Succinate Oxidation ◽  
Intact Cells

Derivatives of 4-(1-adamantyl)-phenol are a promising class of antimicrobials affecting the structural integrity and functions of the bacterial cell membrane. The functioning of Pseudomonas aeruginosa respiratory chain and related system of oxidative phosphorylation was investigated before and after treatment with a derivative of 4-(1-adamantyl)-phenol (compound KVM-97). Oxygen consumption was measured polarographically with a Clark-type oxygen electrode. KVM-97 was tested at 0.5× and 1.0× MIC (minimum inhibitory concentration). Specific substrates of the respiratory chain (either 3.0 mM glutamate with 2.0 mM malonate or 3.0 mM succinate with 5.0 μM rotenone) were used. All reactions were stimulated by addition of ADP (0.2 mmol). It was found that at tested concentrations, KVM-97 inhibited the endogenous respiration and substrate oxidation in P. aeruginosa cells. The inhibiting effect was dose-dependent and more pronounced with succinate oxidation rather than glutamate oxidation. The respiratory control index value (RCI) in compound-treated cells was in average 1.5 times lower compared to the intact cells. The decrease in the RCI was related to changing the oxygen uptake rates in state 3 and state 4, which indicate the uncoupling of respiration and oxidative phosphorylation. The data obtained showed that 4-(1-adamantyl)-phenol derivative inhibits oxygen consumption and has uncoupling effects in P. aeruginosa cells.

scholarly journals Free and Conjugated Benzoic Acid in Tobacco Plants and Cell Cultures. Induced Accumulation upon Elicitation of Defense Responses and Role as Salicylic Acid Precursors

2001 ◽  
Vol 125 (1) ◽  
pp. 318-328 ◽  
Author(s):  
Julie Chong ◽  
Marie-Agnès Pierrel ◽  
Rossitza Atanassova ◽  
Danièle Werck-Reichhart ◽  
Bernard Fritig ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Benzoic Acid ◽  
Cell Cultures ◽  
Defense Responses ◽  
Tobacco Plants

Electron Transport and Coupled Energy Generation in Pseudomonas saccharophila

1971 ◽  
Vol 49 (11) ◽  
pp. 1175-1182 ◽  
Author(s):  
M. Ishaque ◽  
A. Donawa ◽  
M. I. H. Aleem
Keyword(s):  
Oxygen Consumption ◽  
Electron Transport ◽  
Cytochrome C ◽  
Atp Synthesis ◽  
Succinate Oxidation ◽  
Atp Formation ◽  
Low Concentrations ◽  
Oxidase Enzyme ◽  
Succinate Oxidase ◽  
Pseudomonas Saccharophila

The respiratory chain system of heterotrophically grown Pseudomonas saccharophila contained cytochromes of the b, c, a, and o types and also the NADH and succinate oxidase enzyme systems. Cell-free extracts catalyzed phosphorylation coupled to the oxidation of NADH, succinate, and ascorbate (plus cytochrome c). The P/O ratios were in the range of 1.00 for generated NADH, 0.29 for added NADH, 0.50 for succinate, and 0.25 for ascorbate (plus cytochrome c).The oxidative phosphorylation was uncoupled by 2,4-dinitrophenol, 2,6-dibromophenol, pentachlorophenol, m-chlorocarbonyl cyanide phenylhydrazone, and dicumarol without any inhibition of oxygen consumption. Phosphorylation coupled to NADH oxidation was completely inhibited by the flavoprotein inhibitors such as rotenone, amytal, and atabrine; these inhibitors had no effect, however, on the ATP synthesis associated with succinate oxidation. Antimycin A or 2-n-nonyl-4-hydroxyquinoline-N-oxide as well as cyanide or azide at low concentrations completely inhibited the phosphate esterification coupled to the oxidation of NADH or succinate, but had little or no effect on the oxygen consumption. Relatively higher concentrations of oligomycin were required for a complete inhibition of the electron-transport-linked ATP formation.

Réaction du sulfure de bore avec quelques composés difonctionnels

1972 ◽  
Vol 50 (11) ◽  
pp. 1675-1677 ◽  
Author(s):  
S. Jerumanis ◽  
P. A. Begin ◽  
D. Vu Cong
Keyword(s):  
Salicylic Acid ◽  
Succinic Acid ◽  
Phthalic Anhydride ◽  
Maleic Acid ◽  
Phthalic Acid ◽  
Quantitative Yield

Catechol reacts with boron sulfide to give 2,2′-o-phenylenedioxybis-(1,3,2-benzodioxaborole) (1) while only the hydroxyl function of salicylic acid reacts to give a quantitative yield of tris(o-carboxylphenoxy)borane (3). Phthalic acid is dehydrated to phthalic anhydride, but succinic acid and maleic acid have been found inert to the action of the boron sulfide.

scholarly journals Two tobacco genes induced by infection, elicitor and salicylic acid encode glucosyltransferases acting on phenylpropanoids and benzoic acid derivatives, including salicylic acid

FEBS Letters ◽  
1998 ◽  
Vol 437 (3) ◽  
pp. 319-323 ◽  
Author(s):  
Laurence Fraissinet-Tachet ◽  
Rachel Baltz ◽  
Julie Chong ◽  
Serge Kauffmann ◽  
Bernard Fritig ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Benzoic Acid ◽  
Benzoic Acid Derivatives

Simultaneous Gas Chromatographic Determination of Carboxylic Acids in Soft Drinks and Jams

1985 ◽  
Vol 68 (5) ◽  
pp. 902-905
Author(s):  
Taizo Tsuda ◽  
Hiroshi Nakanishi ◽  
Takashi Morita ◽  
Junko Takebayashi
Keyword(s):  
Benzoic Acid ◽  
Succinic Acid ◽  
Tartaric Acid ◽  
Carboxylic Acids ◽  
Fumaric Acid ◽  
Malic Acid ◽  
Sorbic Acid ◽  
Chromatographic Determination ◽  
Dehydroacetic Acid

Abstract A method was developed for simultaneous gas chromatographic determination of sorbic acid, dehydroacetic acid, and benzoic acid used as preservatives, and succinic acid, fumaric acid, malic acid, and tartaric acid used as acidulants in soft drinks and jams. A sample was dissolved in NH4OH-NH4CI pH 9 buffer solution, and an aliquot of the solution was passed through a QAE-Sephadex A 25 column. The column was washed with water, and the carboxylic acids were eluted with 0.1N HC1. Sorbic acid, dehydroacetic acid, and benzoic acid were extracted with ethyl ether-petroleum ether (1 + 1), and determined on a 5% DEGS + 1% H3PO4 column. Succinic acid, fumaric acid, malic acid, and tartaric acid in the lower layer were derivatized with N,0- bis(trimethylsilyl)acetamide and trimethylchlorosilane, and determined on a 3% SE-30 column. Recoveries from soft drink and jam samples fortified with 0.1% each of 7 carboxylic acids ranged from 92.4 to 102.6% for preservatives, and from 88.1 to 103.2% for acidulants.

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