scholarly journals Larval morphology of the family Parthenopidae, with the description of the megalopa stage of Derilambrus angulifrons (Latreille, 1825) (Decapoda: Brachyura), identified by DNA barcode

2014 ◽  
Vol 95 (3) ◽  
pp. 513-521 ◽  
Author(s):  
Elena Marco-Herrero ◽  
J. Ignacio González-Gordillo ◽  
José A. Cuesta
Keyword(s):  
Dna Barcode ◽  
Larval Morphology ◽  
Dna Barcodes ◽  
Previous Description ◽  
Larval Stages ◽  
The Family ◽  
First Time

Although Parthenopidae is a brachyuran decapod family comprising almost 140 species, there is little knowledge about its larval morphology. There are only two complete larval developments reared in the laboratory and some larval stages described for seven species. In the present work these data are compared and analysed. A summary is made of the larval features that characterize parthenopids that can be used to distinguish them from other brachyuran larvae. In addition, the megalopa stage of Derilambrus angulifrons and Parthenopoides massena was collected from plankton and identified by DNA barcodes. The morphology of the megalopa of D. angulifrons is described for the first time, and that of P. massena is compared with a previous description.

scholarly journals A new species of Litarachna Walter, 1925 (Acari: Hydrachnidia: Pontarachnidae) from Corozal Bay (Belize), described based upon morphology and DNA barcodes

Acarologia ◽  
2021 ◽  
Vol 61 (3) ◽  
pp. 602-613
Author(s):  
Lucia Montes-Ortiz ◽  
Tom Goldschmidt ◽  
Lourdes Vásquez-Yeomans ◽  
Manuel Elías-Gutiérrez
Keyword(s):  
Dna Barcode ◽  
Morphological Description ◽  
Estuarine System ◽  
Dna Barcodes ◽  
Sem Images ◽  
The Family ◽  
Coi Sequences ◽  
First Time ◽  
A New Species ◽  
Electronic Microscope

A new planktonic species of the marine water mites of the family Pontarachnidae Koenike, 1910 is described from Corozal Bay, an estuarine system in Belize. The morphological description includes Scanning Electronic Microscope (SEM) images and is augmented by an analysis of DNA cytochrome c oxidase I (COI) sequences, the DNA barcode, used for the first time for a species description in this group.

scholarly journals Designation of a new family group name, Tonzidae fam. nov., for the genus Tonza (Lepidoptera, Yponomeutoidea), based on immature stages of Tonza citrorrhoa

2018 ◽  
Author(s):  
Shigeki Kobayashi ◽  
Haruka Matsuoka ◽  
Masaaki Kimura ◽  
Jae-Cheon Sohn ◽  
Yutaka Yoshiyasu ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Molecular Data ◽  
Systematic Position ◽  
Family Group ◽  
Immature Stages ◽  
Dna Barcodes ◽  
New Family ◽  
The Family ◽  
Family Level ◽  
First Time

The systematic position of Tonza Walker, 1864 is re-evaluated, based on the characteristics of immature stages and DNA barcodes. Larvae and pupae of Tonza citrorrhoa Meyrick, 1905 are described and illustrated for the first time. Larvae of this species form a loose web among the leaves and branches of the host plant, Putranjiva matsumurae Koidz. (Putranjivaceae Endl.). The immature stages of Tonza exhibit four unique apomorphies including: in the larva, the prolegs on A5 and A6 absent, and the seta L2 on the A1–A8 very small; in the pupa, four minute knobs are positioned in the middle portion on abdominal segments V and VI; while its caudal processes possess a W-shaped spine with numerous minute spines. These characteristics clearly distinguish Tonza from other yponomeutoid families and hence, we propose a new family group name, Tonzidae Kobayashi & Sohn fam. nov., for the genus Tonza. Existing DNA barcode data suggest a relationship with Glyphipterigidae Stainton, 1854. The family level status of Tonzidae fam. nov. provides a hypothesis that needs to be tested with larger molecular data.

The complete description of larval stages of the lobster shrimpLeonardsaxius amurensis(Kobjakova, 1937) (Decapoda: Axiidea: Axiidae) identified by DNA barcoding

2017 ◽  
Vol 98 (6) ◽  
pp. 1435-1453 ◽  
Author(s):  
Elena S. Kornienko ◽  
Darya D. Golubinskaya ◽  
Olga M. Korn ◽  
Svetlana N. Sharina
Keyword(s):  
Larval Development ◽  
Sequence Data ◽  
Molecular Genetic ◽  
Sympatric Species ◽  
Molecular Genetic Analysis ◽  
Mitochondrial Coi ◽  
Larval Stages ◽  
The Family ◽  
Rdna Sequence Data ◽  
First Time

The complete larval development of the lobster shrimpLeonardsaxius amurensis(Kobjakova, 1937) (Decapoda: Axiidea: Axiidae) is described and illustrated for the first time. The first zoeae of this species were collected from the plankton samples and reared in the laboratory before moulting to the megalopa. A molecular genetic analysis based on comparison of partial mitochondrial COI, 12S rDNA and 16S rDNA sequence data confirmed the identity of axiid larvae found in the plankton andL. amurensisadults collected in the same area. The larval development ofL. amurensisincludes five zoeal stages and a single megalopa. Zoeae I ofL. amurensisare characterized by the presence of one short posterodorsal spine on the fifth pleonite in contrast to the larvae of related sympatric speciesBoasaxius princepshaving four posterodorsal spines on the pleonites 2–5.Leonardsaxius amurensisoccupies an intermediate position between lobster shrimps with abbreviated pelagic development (2–3 zoeal stages) and species with long development (up to eight zoeal stages). Thus, the number of zoeal stages in the family Axiidae varies widely, similarly to that in the families Callianassidae and Upogebiidae.

New records and their associated DNA barcodes of the butterfly family Hesperiidae in Tibet, China

Zootaxa ◽  
2019 ◽  
Vol 4674 (4) ◽  
pp. 426-438
Author(s):  
CHENGLONG CAO ◽  
SIYAO HUANG ◽  
YONGQIANG XU ◽  
HAOMIN WU ◽  
TIANPENG CHEN ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Morphological Study ◽  
New Records ◽  
Coi Gene ◽  
Dna Barcodes ◽  
Species Cluster ◽  
The Family ◽  
Interspecific Genetic Distance ◽  
Coi Sequences ◽  
First Time

The specimens of the family Hesperiidae collected from Tibet during 2016–2018 are identified using morphology. COI sequences of 76 individuals are newly obtained. The result of our morphological study is congruent with COI gene analyses. Maximum likehood (ML) and Bayesina inferences (BI) analyses reveal that individuals identified morphologically as the same species cluster cohesively. The minimum interspecific genetic distance is 1.7% between Halpe aucma and H. filda, and the genetic distance between conspecific individuals ranged from 0 to 0.2% for the genus Halpe. A total of 51 species are recognized, and six of them, Celaenorrhinus consanguineus Leech, 1891, Barca bicolor (Oberthür, 1896), Aeromachus propinquus Alphéraky, 1897, Pedesta bivitta (Oberthür, 1886), Baoris penicillata chapmani Evans, 1937, and Ochlodes brahma Moore, 1878, are reported from Tibet for the first time, and the last species is new to China. 

Larval morphology and DNA barcodes as valuable tools in early detection of marine invaders: a new pea crab found in European waters

2017 ◽  
Vol 98 (7) ◽  
pp. 1675-1683 ◽  
Author(s):  
Elena Marco-Herrero ◽  
Pilar Drake ◽  
Jose A. Cuesta
Keyword(s):  
Early Detection ◽  
Iberian Peninsula ◽  
Dna Barcoding ◽  
Dna Markers ◽  
Larval Morphology ◽  
Dna Barcodes ◽  
Marine Waters ◽  
Larval Stages ◽  
Unknown Species ◽  
Pea Crab

Four species of Pinnotheridae inhabit European marine waters,Afropinnotheres monodi,Nepinnotheres pinnotheres,Pinnotheres pectunculiandPinnotheres pisum. For these four species there are data available on the morphology of their larval stages as well as DNA markers. This information has allowed us to detect some larvae in plankton samples from the Gulf of Cadiz (SW Iberian Peninsula) that do not belong to any of these European pinnotherid species and to be confirmed by DNA barcoding. In this study these findings are shown as a case of early detection of a newly introduced and unknown species in European marine waters.

scholarly journals Description of three new species of Automeris Hübner, 1819 from Colombia and Brazil (Lepidoptera, Saturniidae, Hemileucinae)

ZooKeys ◽  
2021 ◽  
Vol 1031 ◽  
pp. 183-204
Author(s):  
Thibaud Decaëns ◽  
Frédéric Bénéluz ◽  
Liliana Ballesteros-Mejia ◽  
Diego Bonilla ◽  
Rodolphe Rougerie
Keyword(s):  
New Species ◽  
Dna Barcode ◽  
Comparative Morphology ◽  
Dna Barcodes ◽  
Eastern Cordillera ◽  
Brazilian Amazonia ◽  
Number Of Species ◽  
The Family ◽  
Wing Patterns ◽  
Three New Species

The Saturniidae is one of the most emblematic families of moths, comprising nearly 3000 species distributed globally. In this study, DNA barcode analysis and comparative morphology were combined to describe three new species within the genus Automeris, which is the most diverse genus in the family. Automeris llaneros Decaëns, Rougerie & Bonilla, sp. nov., Automeris mineros Decaëns, Rougerie & Bonilla, sp. nov., and Automeris belemensis Decaëns, Rougerie & Bénéluz, sp. nov. are described from the Colombian Orinoco watershed, the Colombian Eastern Cordillera, and the area of endemism of Belém in the Brazilian Amazonia, respectively. They all belong to the Automeris bilinea (Walker, 1855) species subgroup, which comprises a number of species that are sometimes difficult to distinguish from each other using morphology alone. Here, the description of these three new species is based on significant differences from their closest relatives, either in terms of wing patterns, genitalia, DNA barcodes or a combination of these features.

scholarly journals Revision of the Afrotropical species of the hover fly genus Mesembrius Rondani (Diptera, Syrphidae) using morphological and molecular data

ZooKeys ◽  
2021 ◽  
Vol 1046 ◽  
pp. 1-141
Author(s):  
Kurt Jordaens ◽  
Georg Goergen ◽  
Jeffrey H. Skevington ◽  
Scott Kelso ◽  
Marc De Meyer
Keyword(s):  
Dna Barcode ◽  
Molecular Data ◽  
Junior Synonym ◽  
Dna Barcodes ◽  
Identification Keys ◽  
Males And Females ◽  
Morphological And Molecular Data ◽  
First Time

The Afrotropical representatives of the hover fly genus Mesembrius Rondani, 1857 (Diptera) are divided into two subgenera, namely Mesembrius s.s. and Vadonimyia Séguy, 1951 and, in this present work, the subgenus Mesembrius s.s. is revised. A total of 23 Mesembrius s.s. species are recognised for the Afrotropics. Known species are re-described and six species new to science are described: Mesembrius arcuatussp. nov., M. copelandisp. nov., M. longipilosussp. nov., M. sulcussp. nov., M. tibialissp. nov. and M. vockerothisp. nov. Mesembrius africanus (Verrall, 1898) is considered a junior synonym of M. senegalensis (Macquart, 1842), M. ctenifer Hull, 1941 a junior synonym of M. caffer (Loew, 1858), M. lagopus (Loew, 1869) a junior synonym of M. capensis (Macquart, 1842) and M. platytarsis Curran, 1929 a junior synonym of M. simplicipes Curran, 1929. The females of Mesembrius chapini Curran, 1939, M. rex Curran, 1927 and M. regulus (Hull, 1937) are described for the first time. Lectotypes are designated for Mesembrius caffer, M. capensis, M. cyanipennis (Bezzi, 1915), M. minor (Bezzi, 1915), M. senegalensis, M. strigilatus (Bezzi, 1912) and M. tarsatus (Bigot, 1883). Separate identification keys for males and females are presented. We obtained 236 DNA barcodes for 18 species. The relationships amongst the different Mesembrius species are briefly discussed, based on morphological and DNA barcode data.

Additions and Revisions to the Genera of North American Caddisflies of the Family Brachycentridae With Special Reference to the Larval Stages (Trichoptera)

1965 ◽  
Vol 97 (10) ◽  
pp. 1089-1106 ◽  
Author(s):  
Glenn B. Wiggins
Keyword(s):  
New Species ◽  
North American ◽  
Larval Stage ◽  
New Genus ◽  
New Genera ◽  
Larval Morphology ◽  
Larval Stages ◽  
The North ◽  
The Family ◽  
A New Species

AbstractLarval characterizations for the genera of North American Brachycentridae are revised and two new genera created, bringing to five the total number now recognized on this continent. Previous concepts of larval morphology inMicrasemaare emended. Discovery of the larval stage ofBrachycentrus(Amiocentrus)aspilusRoss necessitates elevation of this subgenus to generic status. Association of larvae with the two North American species placed inOligoplectrumnecessitates removal of one,O.dimicki(Milne), toMicrasema. Discovery of all stages of a new species on Mount Hood, Oregon, necessitates creation of a new genus. A provisional larval key to the genera of the North American Brachycentridae is proposed and the phylogenetic relationships of the genera are discussed. Validity of the Asian genusOligoplectrodesis questioned.

scholarly journals Identification of species and materia medica withinSaussureasubg.Amphilaenabased on DNA barcodes

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6357 ◽  
Author(s):  
Jie Chen ◽  
Yong-Bao Zhao ◽  
Yu-Jin Wang ◽  
Xiao-Gang Li
Keyword(s):  
Internal Transcribed Spacer ◽  
Evolutionary History ◽  
Dna Barcode ◽  
Dna Barcodes ◽  
Additional Species ◽  
Plant Materials ◽  
Materia Medica ◽  
The Family ◽  
Identification Of Species ◽  
Medical Plants

Saussureais one of the most species-rich genera in the family Asteraceae, where some have a complex evolutionary history, including radiation and convergent evolution, and the identification of these species is notoriously difficult. This genus contains many plants with medical uses, and thus an objective identification method is urgently needed.Saussureasubg.Amphilaenais one of the four subgenera ofSaussureaand it is particularly rich in medical resources, where 15/39 species are used in medicine. To test the application of DNA barcodes in this subgenus, five candidates were sequenced and analyzed using 131 individuals representing 15 medical plants and four additional species from this subgenus. Our results suggested that internal transcribed spacer (ITS) +rbcL or ITS +rbcL +psbA-trnH could distinguish all of the species, while the ITS alone could identify all of the 15 medical plants. However, the species identification rates based on plastid barcodes were low, i.e., 0% to 36% when analyzed individually, and 63% when all four loci were combined. Thus, we recommend using ITS +rbcL as the DNA barcode forS.subg.Amphilaenaor the ITS alone for medical plants. Possible taxonomic problems and substitutes for medicinal plant materials are also discussed.

scholarly journals First Descriptions of Larva and Pupa of Bagous claudicans Boheman, 1845 (Curculionidae, Bagoinae) and Systematic Position of the Species Based on Molecular and Morphological Data

Insects ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 166
Author(s):  
Rafał Gosik ◽  
Miłosz A. Mazur ◽  
Natalia Sawka-Gądek
Keyword(s):  
Feeding Habits ◽  
Morphological Characters ◽  
Systematic Position ◽  
Morphological Data ◽  
Larval Morphology ◽  
Mature Larva ◽  
Larval Stages ◽  
First Time ◽  
Specimen Identification ◽  
First Descriptions

In this paper, the mature larva and pupa of Bagous claudicans are described and illustrated for the first time. Measurements of younger larval instars are also given. The biology of the species is discussed in association with larval morphology and feeding habits. Overall larval and pupal morphological characters of the genus Bagous are presented. Confirmation of the larva identification as Bagous claudicans species was conducted by cytochrome oxidase I (COI) sequencing. DNA barcoding was useful for specimen identification of larval stages. The systematic position of the species within the Bagous collignensis-group, based on morphological and molecular results, is also discussed.

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