Larval morphology and DNA barcodes as valuable tools in early detection of marine invaders: a new pea crab found in European waters

2017 ◽  
Vol 98 (7) ◽  
pp. 1675-1683 ◽  
Author(s):  
Elena Marco-Herrero ◽  
Pilar Drake ◽  
Jose A. Cuesta
Keyword(s):  
Early Detection ◽  
Iberian Peninsula ◽  
Dna Barcoding ◽  
Dna Markers ◽  
Larval Morphology ◽  
Dna Barcodes ◽  
Marine Waters ◽  
Larval Stages ◽  
Unknown Species ◽  
Pea Crab

Four species of Pinnotheridae inhabit European marine waters,Afropinnotheres monodi,Nepinnotheres pinnotheres,Pinnotheres pectunculiandPinnotheres pisum. For these four species there are data available on the morphology of their larval stages as well as DNA markers. This information has allowed us to detect some larvae in plankton samples from the Gulf of Cadiz (SW Iberian Peninsula) that do not belong to any of these European pinnotherid species and to be confirmed by DNA barcoding. In this study these findings are shown as a case of early detection of a newly introduced and unknown species in European marine waters.

scholarly journals Exploring unknown life stages of Arctic Tanytarsini (Diptera: Chironomidae) with DNA barcoding

Zootaxa ◽  
2011 ◽  
Vol 2743 (1) ◽  
pp. 27 ◽  
Author(s):  
ELISABETH STUR ◽  
TORBJØRN EKREM
Keyword(s):  
Dna Barcoding ◽  
Coi Gene ◽  
Morphological Identification ◽  
Life Stages ◽  
Larval Morphology ◽  
Gene Sequences ◽  
Larval Stages ◽  
Identification Keys ◽  
Close Resemblance ◽  
First Results

We present the first results from a project on Spitsbergen and Bear Island where the ultimate goal is to provide genetic and morphological identification keys to all species. Five Tanytarsini species have been recorded from Svalbard. By sequencing partial COI gene sequences, we have associated larvae of all of these, and describe the hitherto undescribed larval stages of Microspectra insignilobus Kieffer, M. radialis Goetghebuer and Tanytarsus heliomesonyctios Langton. We also present keys to larva, pupa and imagines of all Tanytarsini species found on Svalbard and discuss larval morphology in relationship to characters preserved in subfossil material. Of particular interest is the mandible and mentum of T. heliomsonyctios which show close resemblance to the tentatively associated larvae of Corynocera oliveri Lindeberg. An overview over Tanytarsus species with “lugens-type” larvae is given and the phylogenetic value of mandibular accessory teeth is briefly discussed.

scholarly journals Identification of Nepeta olgae Regel and phylogenetic status of some genera in subtribe Nepetinae (Lamiaceae) using DNA markers

2021 ◽  
Vol 38 ◽  
pp. 00087
Author(s):  
Elena Nikitina ◽  
Abdurashid Rakhmatov
Keyword(s):  
Dna Barcoding ◽  
Large Scale ◽  
Dna Barcode ◽  
Dna Barcodes ◽  
Biodiversity Monitoring ◽  
Reference Library ◽  
Accurate Identification ◽  
Unknown Species ◽  
Phylogenetic Status ◽  
Nuclear Its

The species level diversity is the reference unit for biodiversity accounting, should be systematized and include full information about the species. Reliable identification of any species is critical for a large-scale biodiversity monitoring and conservation. A DNA barcode is a DNA sequence that identifies a species by comparing the sequence of an unknown species with barcodes of a known species sequence database. Accurate identification of important plants is essential for their conservation, inventory. The species diversity assessing exampled on the subtribe Nepetinae (Lamiaceae) representatives, growing in Uzbekistan is given, using DNA barcoding method. The study was aimed to identify indigenous important plants with the nuclear (ITS) and plastid (matK, rbcL, trnL-F) genomes. This work demonstrates the phylogenetic relationships of some genera within the subtribe Nepetinae Coss. & Germ. (Lamiaceae), based on ITS locus gene. All results indicate that the DNA barcoding tool can be successfully used to reliably identify important plants, to inventory the botanical resources of Uzbekistan and to create a reference library of DNA barcodes. So, the combination of three-four locus gene is a good candidate for this approach.

scholarly journals A new species of pea crab from south-western Europe (Crustacea, Decapoda, Brachyura): species description, geographic distribution and population structure with an identification key to European Pinnotheridae

2019 ◽  
Vol 99 (5) ◽  
pp. 1141-1152 ◽  
Author(s):  
Jose A. Cuesta ◽  
J. E. García Raso ◽  
Pere Abelló ◽  
Elena Marco-Herrero ◽  
Luis Silva ◽  
...  
Keyword(s):  
New Species ◽  
Geographic Distribution ◽  
Western Europe ◽  
Female Size ◽  
Gulf Of Cadiz ◽  
Larval Stages ◽  
Unknown Species ◽  
Gulf Of Cádiz ◽  
Pea Crab ◽  
A New Species

AbstractAfter the recent detection, by both morphology and DNA barcodes, of the larval stages of an unknown species of pea crab (Pinnotheres sp.) in European waters, adults of this crab are herein reported and described as a new species. The current known geographic distribution of the species comprises the Gulf of Cádiz in the eastern Atlantic and the adjacent Mediterranean waters of the south of the Iberian Peninsula (Alboran Sea), where this crab is well-established inside the anomiid bivalve Anomia ephippium. In the Gulf of Cádiz, the species displayed a relatively high prevalence: on average, 55.6–77.7%, in A. ephippium samples. The dominant demographic categories of the new species were soft females (61.8–77.0%) with fewer males (17.7–21.10%). Most of the host bivalves carried only one crab; in bivalves harbouring two crabs, heterosexual pairs were collected more frequently than expected by chance, which suggests that they could be mated pairs. A strong correlation between host size and soft female size was found (r = 0.73, P < 0.01) indicating that space availability within hosts seems to be relevant in determining the size of the sedentary phase of the new crab species.

scholarly journals Larval morphology of the family Parthenopidae, with the description of the megalopa stage of Derilambrus angulifrons (Latreille, 1825) (Decapoda: Brachyura), identified by DNA barcode

2014 ◽  
Vol 95 (3) ◽  
pp. 513-521 ◽  
Author(s):  
Elena Marco-Herrero ◽  
J. Ignacio González-Gordillo ◽  
José A. Cuesta
Keyword(s):  
Dna Barcode ◽  
Larval Morphology ◽  
Dna Barcodes ◽  
Previous Description ◽  
Larval Stages ◽  
The Family ◽  
First Time

Although Parthenopidae is a brachyuran decapod family comprising almost 140 species, there is little knowledge about its larval morphology. There are only two complete larval developments reared in the laboratory and some larval stages described for seven species. In the present work these data are compared and analysed. A summary is made of the larval features that characterize parthenopids that can be used to distinguish them from other brachyuran larvae. In addition, the megalopa stage of Derilambrus angulifrons and Parthenopoides massena was collected from plankton and identified by DNA barcodes. The morphology of the megalopa of D. angulifrons is described for the first time, and that of P. massena is compared with a previous description.

scholarly journals Peering into the Darkness: DNA Barcoding Reveals Surprisingly High Diversity of Unknown Species of Diptera (Insecta) in Germany

Insects ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 82
Author(s):  
Caroline Chimeno ◽  
Axel Hausmann ◽  
Stefan Schmidt ◽  
Michael J. Raupach ◽  
Dieter Doczkal ◽  
...  
Keyword(s):  
Species Richness ◽  
Dna Barcoding ◽  
Dna Barcodes ◽  
Insect Fauna ◽  
Southern Germany ◽  
Unknown Species ◽  
High Diversity ◽  
Malaise Traps

Determining the size of the German insect fauna requires better knowledge of several megadiverse families of Diptera and Hymenoptera that are taxonomically challenging. This study takes the first step in assessing these “dark taxa” families and provides species estimates for four challenging groups of Diptera (Cecidomyiidae, Chironomidae, Phoridae, and Sciaridae). These estimates are based on more than 48,000 DNA barcodes (COI) from Diptera collected by Malaise traps that were deployed in southern Germany. We assessed the fraction of German species belonging to 11 fly families with well-studied taxonomy in these samples. The resultant ratios were then used to estimate the species richness of the four “dark taxa” families (DT families hereafter). Our results suggest a surprisingly high proportion of undetected biodiversity in a supposedly well-investigated country: at least 1800–2200 species await discovery in Germany in these four families. As this estimate is based on collections from one region of Germany, the species count will likely increase with expanded geographic sampling.

scholarly journals DNA Barcoding of Endangered Paphiopedilum species (Orchidaceae) of Peninsular Malaysia

Phytotaxa ◽  
2019 ◽  
Vol 387 (2) ◽  
pp. 94-104 ◽  
Author(s):  
MICHEAL C. RAJARAM ◽  
CHRISTINA S.Y. YONG ◽  
JUALANG A. GANSAU ◽  
RUSEA GO
Keyword(s):  
Phylogenetic Tree ◽  
Dna Barcoding ◽  
Dna Markers ◽  
High Accuracy ◽  
Peninsular Malaysia ◽  
Dna Barcodes ◽  
Accurate Identification ◽  
Barcoding Gap ◽  
Preliminary Identification ◽  
Sequence Quality

In this study, the efficacy of four DNA markers and their combinations (rbcL, matK, ITS, trnH-psbA) as barcode markers were tested across the endangered Paphiopedilum species from Peninsular Malaysia. Four species of Paphiopedilum were sampled and barcoded. The DNA barcodes reliabilities were evaluated using NCBI BLASTn program, phylogenetic tree via Neighbour-Joining method with 1000 bootstrap replicates in MEGA 6 and barcoding gap assessment. matK is the most promising barcode with high sequence quality (100%), high accuracy in BLASTn (100%), clear resolution of species in Neighbour-Joining phylogenetic tree (100%) and a distinct barcoding gap followed by ITS, trnH-psbA and rbcL. The combination of barcode regions revealed the lack of variation in rbcL and trnH-psbA but they are still useful for preliminary identification followed up by matK for accurate identification.

scholarly journals Benchmarking DNA barcodes: an assessment using available primate sequences

Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 851-854 ◽  
Author(s):  
Mehrdad Hajibabaei ◽  
Gregory AC Singer ◽  
Donal A Hickey
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Species Identification ◽  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Primate Species ◽  
Dna Barcodes ◽  
Global Biodiversity ◽  
Efficient Sequence

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.

scholarly journals First record of roving coral grouper Plectropomus pessuliferus (Fowler, 1904) from Saint Martin’s Island, Bangladesh based on Morphology and DNA Barcoding

2021 ◽  
Vol 32 (2) ◽  
pp. 293-301
Author(s):  
MD JAYEDUL ISLAM ◽  
SHARMIN AKTER ◽  
PROVAKOR SARKAR ◽  
MOHAMMAD RASHED ◽  
IREEN PARVIN ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Bay Of Bengal ◽  
New Record ◽  
First Record ◽  
Morphological Characters ◽  
Marine Waters ◽  
First Report ◽  
Saint Martin ◽  
Northern Bay Of Bengal

A new record of Plectropomus pessuliferus (Serranidae: Epinephelinae) wasdocumented based on morphological characters and DNA barcoding. The species was collectedduring a regular survey for making an inventory of reef associated fishes in Saint Martin`sIsland, Bangladesh. This is the first report of roving coral grouper from the marine waters ofBangladesh validated by morpho-meristic analysis and DNA barcoding. This is also the firstreport from the northern Bay of Bengal.

scholarly journals Parasitic wasps as natural enemies of aphid populations in the Mashhad region of Iran: New data from DNA barcodes and SEM

2011 ◽  
Vol 63 (4) ◽  
pp. 1225-1234 ◽  
Author(s):  
Reyhaneh Darsouei ◽  
Javad Karimi ◽  
Mehdi Modarres-Awal
Keyword(s):  
Dna Barcoding ◽  
Modern Method ◽  
Diaeretiella Rapae ◽  
Coi Gene ◽  
Dna Barcodes ◽  
Parasitic Wasps ◽  
Pome Fruit ◽  
Initial Attempt ◽  
Morphometric Characters ◽  
Preliminary Identification

DNA barcoding is a modern method for the identification of different species, including insects. Among animals, the major emphasis of DNA barcoding is on insects. Due to this global trend we addressed this approach for surveying a group of insects. The parasitic wasps (including primary and hyperparasitoids) of pome fruit orchard aphids were collected from Iran-Mashhad during 2009-2010. Preliminary identification of this group was performed by using morphological and morphometric characters and SEM. The COI gene in the specimens was amplified and sequenced. In this survey, Aphidius matricariae, Binodoxys angelicae, Diaeretiella rapae, Ephedrus persicae, Lysiphlebus fabarum and Praon volucre parasitoids and Alloxysta sp., Asaphes suspensus, Dendrocerus carpenteri, Pachyneuron aphidis, Syrphophagus aphidivorus hyperparasitoids were studied. Based on intra-interspecies distances and phylogenetic analysis using NJ, all species possess diagnostic barcode sequences. The results of this study show that the COI sequence could be useful in identification study of this group of insects. Here we have provided the first GenBank data for the COI gene of the above-mentioned hyperparasitoids as well as an initial attempt toward preparing DNA barcodes for Iranian parasitoid and hyperparasitoid aphids.

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