Suppression of expulsion ofAspiculuris tetrapterain hydrocortisone and methotrexate treated mice

Hydrocortisone treated male and female mice, given a primary infection withAspiculuris tetraptera, did not reject the worms during the third week of infection. Mice given hydrocortisone during the first week of infection had elevated worm burdens on day 10, suggesting that some worm loss was encountered during the anterior migration in control mice. Furthermore, this temporary period of treatment was sufficient totally to suppress rejection and to allow the parasite to persist until day 28. Methotrexate also significantly delayed rejection, but larval growth was retarded in treated mice. These results, it is suggested, add strength to the hypothesis that the loss ofA. tetrapterain a primary infection in mice, is an immunological phenomenon.

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Aspiculuris tetraptera in wild Mus musculus. The prevalence of infection in male and female mice

Journal of Helminthology ◽

10.1017/s0022149x0002318x ◽

1975 ◽

Vol 49 (2) ◽

pp. 85-90 ◽

Cited By ~ 22

Author(s):

Jerzy M. Behnke

Keyword(s):

Small Mammals ◽

Mus Musculus ◽

Mouse Population ◽

Male And Female ◽

Female Mice ◽

Distribution Studies ◽

Syphacia Obvelata ◽

Regent's Park ◽

Aspiculuris Tetraptera ◽

Wild House Mouse

AbstractA survey was carried out of the levels of infection with Aspiculuris tetraptera and Syphacia obvelata in a wild house mouse population living in the Charles Clore Small Mammals Pavilion at the London Zoo in Regent's Park. The extent of infection with A. tetraptera is analysed according to the sex of the host. It is shown that the prevalence of infection was greater in male than in female mice and frequency distribution studies suggest that this is not only because fewer female mice become infected but also because females resist larvae more effectively than do males.

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Concomitant development of oral dyskinesia and memory deficits in reserpine-treated male and female mice

Behavioural Brain Research ◽

10.1016/s0166-4328(01)00409-0 ◽

2002 ◽

Vol 132 (2) ◽

pp. 171-177 ◽

Cited By ~ 41

Author(s):

Regina H Silva ◽

Vanessa C Abı́lio ◽

Danila Torres-Leite ◽

Marcelo Bergamo ◽

Cibele C Chinen ◽

...

Keyword(s):

Memory Deficits ◽

Male And Female ◽

Treated Male ◽

Female Mice ◽

Oral Dyskinesia

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Herpes simplex virus infections in male and female mice following pinna inoculation: Responses to primary infection and artificially induced recurrent disease

Journal of Medical Virology ◽

10.1002/jmv.1890290419 ◽

1989 ◽

Vol 29 (4) ◽

pp. 320-326 ◽

Cited By ~ 10

Author(s):

Joseph M. Blondeau ◽

Juan A. Embil ◽

E. Sandra McFarlane

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Primary Infection ◽

Recurrent Disease ◽

Virus Infections ◽

Male And Female ◽

Female Mice ◽

Simplex Virus

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Induction of murine α-foetoprotein synthesis by oestradiol

Acta Endocrinologica ◽

10.1530/acta.0.1060141 ◽

1984 ◽

Vol 106 (1) ◽

pp. 141-144 ◽

Cited By ~ 5

Author(s):

J. Hau ◽

J. Chemnitz ◽

B. Teisner ◽

D. Tornehave ◽

P. Svendsen

Keyword(s):

Liver Tissue ◽

Late Pregnancy ◽

Serum Levels ◽

Immunohistochemical Examination ◽

Male And Female ◽

Treated Male ◽

Female Mice ◽

Adult Mice

Abstract. The synthesis of murine α-foetoprotein (m-AFP) was induced in 43 out of 47 adult mice of both sexes by sc administration of 100 μg oestradiol-17β every second day. The m-AFP serum levels of the oestrogen treated male and female mice were 7 and 17%, respectively, of the levels in mice during late pregnancy. Immunohistochemical examination of liver tissue revealed the intracellular presence of m-AFP in less than 0.5% of the hepatocytes scattered throughout the liver of the oestrogen treated mice.

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12 Sex-related differential susceptibility to ponatinib-induced cardiotoxicity and its relationship to modulation of notch signalling in a muine model

European Heart Journal Supplements ◽

10.1093/eurheartj/suab130.005 ◽

2021 ◽

Vol 23 (Supplement_G) ◽

Author(s):

Rosalinda Madonna ◽

Damiana Pieragostino ◽

Maria Concetta Cufaro ◽

Piero Del Boccio ◽

Angela Pucci ◽

...

Keyword(s):

Cell Death ◽

Cardiac Fibrosis ◽

Kinase Inhibitor ◽

Left Ventricular Systolic Dysfunction ◽

Differential Susceptibility ◽

Left Ventricular ◽

Male Mice ◽

Male And Female ◽

Treated Male ◽

Female Mice

Abstract Aims Ponatinib (PON), a tyrosine kinase inhibitor approved in chronic myeloid leukaemia, has proven cardiovascular toxicity. Although sex is a risk factor for PON-induced cardiotoxicity in humans, little is known about its mechanisms, in general, and sex-related mechanisms in particular. To determine the mechanisms of sex-related PON-induced cardiotoxicity and identify potential rescue strategies in a murine model. Methods Twenty-four-month-old male and female C57B5 mice were treated with 3 mg/kg/day of PON or vehicle via oral gavage for 28 days, with/without siRNA-Notch1 or siRNA-scrambled via tail vein every 3 days. Results PON + scrambled siRNA-treated male mice had a higher number of TUNEL-positive cells, a higher percentage of senescence-associated β-galactosidase positive senescent cardiac areas, as well as a lower reactivity degree for the survival marker Bmi1 than female counterparts. Proteomics analysis of cardiac tissue showed upstream activation of nitric oxide synthase (NOS) type 2, downstream activation of cell death and production of reactive oxygen species in PON + scrambled siRNA- compared to vehicle or PON + Notch1 siRNA-treated male mice. Upstream analysis showed beta-estradiol activation, while downstream analysis showed activation of cell survival and inhibition of cell death in PON + scrambled siRNA compared to vehicle-treated female mice. PON + scrambled siRNA-treated mice also showed a down-regulation of cardiac actin, which was more marked in male; as well as vessel density, which was more marked in female mice. Female hearts showed a greater extent of cardiac fibrosis than male counterparts at baseline, with no significant changes after PON treatment. In contrast, PON + scrambled siRNA-treated mice had less fibrosis than vehicle or PON + Notch1-siRNA-treated mice. Left ventricular systolic dysfunction shown in PON + scrambled siRNA-treated male mice and—to a lesser extent—by female mice was similarly reversed in both PON + Notch1-siRNA-treated male and female mice (Table 1). Conclusions We found a sex-related differential susceptibility and Notch1 modulation in PON-induced cardiotoxicity. This can improve our understanding of sex-related differences and help identify biomarkers in PON cardiotoxicity.

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Sex Differences in Animal Models of Sodium-Valproate-Induced Autism in Postnatal BALB/c Mice: Whole-Brain Histoarchitecture and 5-HT2A Receptor Biomarker Evidence

Biology ◽

10.3390/biology11010079 ◽

2022 ◽

Vol 11 (1) ◽

pp. 79

Author(s):

Balaji Gouda ◽

Sukesh Narayan Sinha ◽

Meram Chalamaiah ◽

Validandi Vakdevi ◽

Patangay Shashikala ◽

...

Keyword(s):

Cerebral Cortex ◽

Purkinje Cells ◽

Morphological Changes ◽

Autism Spectrum ◽

Nervous System Development ◽

Receptor Protein ◽

Morphological Observation ◽

Male And Female ◽

Treated Male ◽

Female Mice

Autism spectrum disorder (ASD) is characterised by problems with social interaction, verbal and nonverbal communication and repetitive behaviour. In mice, the 14th postnatal day is believed to correspond to the third trimester of human embryonic development and is considered a vital period for central nervous system development. It has been shown that ASD affects 2 to 3 times more male than female individuals. In the present study, ASD was induced in 14 postnatal day (PND) BALB/c mice using valproic acid (VPA). VPA administration brought about substantial differences in the histoarchitecture of the brain in both male and female mice, linked to behavioural deficits. We observed that both male and female mice showed similar morphological changes in the prefrontal cortex, hippocampus and Purkinje cells. We also observed hair loss from PND 17 to 25, which was again similar between male and female mice. However, there were higher rates of change in the cerebral cortex, frontal cortex and temporal lobe and hippocampus in VPA-treated male animals. With respect to the cerebellum, we did not observe any alterations by haematoxylin and eosin (H&E) staining, but detailed morphological observation using scanning electron microscopy (SEM) showed a higher rate of phenotype changes in VPA-treated male animals. Moreover, 5-HT2A receptor protein levels were upregulated in the cerebral cortex, hippocampus and Purkinje cells in VPA-treated male mice compared with control animals and VPA-treated female mice, as shown by immunohistochemical analysis. Based on all these findings, we conclude that male animals are more susceptible to VPA-induced ASD than females.

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Sex-related differential susceptibility to ponatinib-induced cardiotoxicity and its relationship to modulation of Notch signaling in a murine model

European Heart Journal ◽

10.1093/eurheartj/ehab724.3266 ◽

2021 ◽

Vol 42 (Supplement_1) ◽

Author(s):

R Madonna ◽

D Pieragostino ◽

M C Cufaro ◽

P Del Boccio ◽

A Pucci ◽

...

Keyword(s):

Cell Death ◽

Murine Model ◽

Kinase Inhibitor ◽

Left Ventricular Systolic Dysfunction ◽

Differential Susceptibility ◽

Left Ventricular ◽

Male Mice ◽

Male And Female ◽

Treated Male ◽

Female Mice

Abstract Background Ponatinib (PON), a tyrosine kinase inhibitor approved in chronic myeloid leukemia, has proven cardiovascular toxicity. Although sex is a risk factor for PON-induced cardiotoxicity in humans, little is known about its mechanisms in general, and sex-related mechanisms in particular. Objectives To determine the mechanisms of sex-related PON-induced cardiotoxicity and identify potential rescue strategies in a murine model. Methods 24-months-old male and female C57B5 mice were treated with 3 mg/kg/day of PON or vehicle via oral gavage for 28 days, with/without siRNA-Notch1 or siRNA-scrambled via tail vein every 3 days. Results PON + scrambled siRNA-treated male mice had a higher number of TUNEL-positive cells, a higher percentage of senescence-associated β-galactosidase positive senescent cardiac areas, as well as a lower reactivity degree for the survival marker Bmi1 than female counterparts. Proteomics analysis of cardiac tissue showed upstream activation of nitric oxide synthase (NOS) type 2, downstream activation of cell death and production of reactive oxygen species in PON + scrambled siRNA- compared to vehicle or PON + Notch1 siRNA-treated male mice. Upstream analysis showed beta-estradiol activation, while downstream analysis showed activation of cell survival and inhibition of cell death in PON + scrambled siRNA compared to vehicle-treated female mice. PON + scrambled siRNA-treated mice also showed a downregulation of cardiac actin, which was more marked in male; as well as vessel density, which was more marked in female mice. Female hearts showed a greater extent of cardiac fibrosis than male counterparts at baseline, with no significant changes after PON treatment. In contrast, PON + scrambled siRNA-treated mice had less fibrosis than vehicle or PON + Notch1-siRNA-treated mice. Left ventricular systolic dysfunction shown in PON + scrambled siRNA-treated male mice and - to a lesser extent - by female mice was similarly reversed in both PON + Notch1-siRNA-treated male and female mice (Table 1). Conclusions We found a sex-related differential susceptibility and Notch1 modulation in PON-induced cardiotoxicity. This can improve our understanding of sex-related differences and help identify biomarkers in PON cardiotoxicity. FUNDunding Acknowledgement Type of funding sources: None.

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