The potential of mass spectrometry for the global profiling of parasite metabolomes

Parasitology ◽  
2009 ◽  
Vol 137 (9) ◽  
pp. 1409-1423 ◽  
Author(s):  
D. G. WATSON

SUMMARYThe strengths and limitations of existing mass spectrometry methods for metabolite detection and identification are discussed. A brief review is made of the methods available for quenching and extraction of cells or organisms prior to instrumental analysis. The techniques available for carrying out mass spectrometry-based profiling of metabolomes are discussed using the analysis of extracts from trypanosomes to illustrate various points regarding methods of separation and mass spectrometric analysis. The advantages of hydrophilic interaction chromatography (HILIC) for the analysis of polar metabolites are discussed. The challenges of data processing are outlined and illustrated using the example of ThermoFisher's Sieve software. The existing literature on applications of mass spectrometry to the profiling of parasite metabolomes is reviewed.

2015 ◽  
Vol 7 (23) ◽  
pp. 9808-9816 ◽  
Author(s):  
Steven L. Reeber ◽  
Sneha Gadi ◽  
Sung-Ben Huang ◽  
Gary L. Glish

Paper spray ionization enables the rapid mass spectrometric analysis of environmental samples without the use of chromatography or sample cleanup techniques.


The Analyst ◽  
2016 ◽  
Vol 141 (19) ◽  
pp. 5520-5526 ◽  
Author(s):  
Eric Janusson ◽  
G. Bryce McGarvey ◽  
Farhana Islam ◽  
Christine Rowan ◽  
J. Scott McIndoe

A simple chemical derivatization technique was developed for electrospray ionization mass spectrometry (ESI-MS) in which thiols and disulfides may be selectively analyzed in a complex matrix and easily characterized.


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