Relationship between in vitro gas production and in situ degradability for forage components

1998 ◽  
Vol 22 ◽  
pp. 244-246
Author(s):  
T. Ichinohe ◽  
W. J. Shand ◽  
D. J. Kyle ◽  
X. B. Chen ◽  
E. R. Ørskov
Keyword(s):  
Production Rate ◽  
Nutritive Value ◽  
Kinetic Studies ◽  
Gas Production ◽  
Soluble Carbohydrates ◽  
In Vitro Gas Production ◽  
The Relationship

Recently in vitro and in situ techniques have been used for assessing forage nutritive value for ruminants instead of laborious in vivo trials. Although, Blummel and Ørskov (1993) have shown that an in vitro gas production technique gives reliable estimates of forage nutritive value, whether the estimates could be correlated with in situ degradation are not well established. There is little known about the differences in degradation characteristics of neutral-detergent fibre (NDF) and cellular contents (CC: soluble carbohydrates, lipids and crude protein) in the rumen, and also about the relationships between component degradation rate in the rumen and gas production rate for different forages. This is because most in situ kinetic studies have followed the disappearance of insoluble cell wall constituents and comparative studies of in vitro and in situ estimates was not performed sufficiently.The objective of this experiment was to investigate the relationship between in situ degradation of forage components and in vitro gas production rate for the three forages.

Rumen degradability of Mongolian pastures: a comparison of in situ and in vitro gas production techniques

1996 ◽  
Vol 1996 ◽  
pp. 227-227 ◽  
Author(s):  
A.H. Murray ◽  
D. Daalkhaijav ◽  
C.D. Wood
Keyword(s):  
Gas Production ◽  
Forest Steppe ◽  
High Mountain ◽  
In Vitro Gas Production ◽  
Production Techniques ◽  
Native Pastures ◽  
The Relationship

In Mongolia animal performance is very much dependent on the quality and quantity of natural grassland available, since certain pastoral animals may obtain as much as 98% of their annual intake from pasture. There have been few studies to date on either in vivo or in vitro degradation of native Mongolian pastures. This paper seeks to investigate the degradation characteristics of native pastures from 2 regions in Mongolia, high mountain and forest steppe. It also investigates the relationship between the in vitro gas production technique and the in sacco technique.

Relationship between in vitro gas production and near infrared reflectance spectra of gliricidia provenances

1996 ◽  
Vol 1996 ◽  
pp. 226-226
Author(s):  
S.J. Lister ◽  
M.S. Dhanoa ◽  
J.L. Stewart ◽  
M. Gill
Keyword(s):  
Nutritive Value ◽  
Near Infrared ◽  
Gas Production ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance ◽  
In Vitro Gas Production ◽  
The Relationship ◽  
Kinetics Of

The chemical composition of tropical forages is generally more complex than that of temperate forages, yet the need for simple methods to predict nutritive value is greater. Near Infrared Reflectance (NIR) spectra could provide a possible solution. NIR has been used to predict both in vitro and in vivo digestibility (Murray, 1993). Direct calibration with in vivo parameters is ideal, but factors such as different types of forages, animals and management conditions have to be taken into consideration. The gas production technique, which may be considered as an in vitro counterpart of the dacron bag technique, provides information relating to the fermentable fractions and kinetics of a forage and these parameters can be related to the NIR spectra. The aim of this study was to investigate the relationship between NIR spectra and cumulative gas production and derived parameters in tree leaves.

Relationship between in vitro gas production and near infrared reflectance spectra of gliricidia provenances

1996 ◽  
Vol 1996 ◽  
pp. 226-226
Author(s):  
S.J. Lister ◽  
M.S. Dhanoa ◽  
J.L. Stewart ◽  
M. Gill
Keyword(s):  
Nutritive Value ◽  
Near Infrared ◽  
Gas Production ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance ◽  
In Vitro Gas Production ◽  
The Relationship ◽  
Kinetics Of

The chemical composition of tropical forages is generally more complex than that of temperate forages, yet the need for simple methods to predict nutritive value is greater. Near Infrared Reflectance (NIR) spectra could provide a possible solution. NIR has been used to predict both in vitro and in vivo digestibility (Murray, 1993). Direct calibration with in vivo parameters is ideal, but factors such as different types of forages, animals and management conditions have to be taken into consideration. The gas production technique, which may be considered as an in vitro counterpart of the dacron bag technique, provides information relating to the fermentable fractions and kinetics of a forage and these parameters can be related to the NIR spectra. The aim of this study was to investigate the relationship between NIR spectra and cumulative gas production and derived parameters in tree leaves.

scholarly journals Determination of the In Vitro Gas Production and Potential Feed Value of Olive, Mulberry and Sour Orange Tree Leaves

2018 ◽  
Vol 13 (1) ◽  
pp. 269-278 ◽  
Author(s):  
Mustafa Olfaz ◽  
Unal Kilic ◽  
Mustafa Boga ◽  
Abdiwali Mohamoud Abdi
Keyword(s):  
Nutritive Value ◽  
Condensed Tannin ◽  
Gas Production ◽  
Net Energy ◽  
Sour Orange ◽  
Tree Leaves ◽  
Randomized Design ◽  
In Vitro Gas Production

AbstractThis study was conducted to determine the potential nutritive value andin vitrogas production (IVGP) parameters ofOlea europaea L. (Olive = OL),Morus alba L. (Mulberry = ML) andCitrus aurantium L. (Sour orange = SOL) tree leaves. Hohenheim gas test was used to determine thein vitrogas productions of the leaves. The gas production of samples over time was recorded for 3, 6, 9, 12, 24, 48, 72 and 96 h after incubation. Completely Randomized Design was used to compare gas production, and gas production kinetics of samples. The findings of the present study suggested that there were differences among the tree leaves in terms of crude protein, NDF,in vitrogas productions, organic matter digestibility (OMD), metabolisable energy (ME), net energy lactation (NEL) and relative feed values (RFV) (P<0.01). ML had the highest condensed tannin contents (P<0.05),in vitrogas production (IVGP), OMD and energy values (P<0.01). SOL had highest RFV values. OL showed the lowest IVGP when compared to SOL and ML. Low NDF and ADF contents of SOL would probably increase the voluntary intake, digestibility and relative feed values of these leaves by ruminants. In conclusion, it was determined that OL, ML and SOL used in the study have lowin vitrogas production and can be utilized as alternative roughage feed in ruminants. However, it is recommended that the results obtained from this research should be tested inin vivostudies.

Palatability,in situandin vitronutritive value of dried sainfoin (Onobrychis viciifolia)

2010 ◽  
Vol 148 (6) ◽  
pp. 723-733 ◽  
Author(s):  
H. KHALILVANDI-BEHROOZYAR ◽  
M. DEHGHAN-BANADAKY ◽  
K. REZAYAZDI
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Nutritive Value ◽  
Nutrient Content ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Acid Detergent Fibre

SUMMARYThe current study was conducted to determine chemical composition, nutrient content and availability, metabolizable energy (ME) content and nutritive value of sainfoin hay for ruminants. Three ruminally cannulated Holstein cows were used forin situandin vivoexperiments, to determine rumen degradability and digestibility of sainfoin hay. Apparent total tract digestibility of nutrients was determined with feeding of sainfoin hay as the sole diet to achieve 10% more than maintenance energy requirements. Six Zandi ewes were used in the palatability experiment. Means for dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF) and condensed tannins (CTs) of sainfoin hay were: 940·4 g/kg and 93·43, 12·13, 47·87, 43·33 and 2·13 g/kg DM, respectively.In situeffective degradability of CP and DM were 0·38 and 0·54 g/g with a ruminal outflow rate of 0·05/h, respectively. OM apparent digestibility was in the range of 0·592–0·689, respectively, for Tilley & Terry and total faecal collection assays. ME content of sainfoin hay, according to different methods (gas production,in vitroandin vivodetermined digestible organic matter in dry matter (DOMD)) was in the range 6·87–10·11 MJ/kg DM. Metabolizable protein (MP) content was 483·4 g/kg CP. Sainfoin was more palatable than alfalfa for sheep. It was concluded that sainfoin has a potential use in ruminant rations, especially if environmental conditions are not suitable for alfalfa production.

Relationship between in vitro gas production and in vivo energy digestibility in growing pigs

1996 ◽  
Vol 1996 ◽  
pp. 142-142
Author(s):  
A.C. Longland ◽  
S.P. Bray ◽  
A.E. Brooks ◽  
M.K. Theodorou ◽  
A.G. Low
Keyword(s):  
Pressure Transducer ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Growing Pigs ◽  
Digestible Energy ◽  
In Vitro Gas Production

In vitro methods which can be used to predict the nutritive value of feedstuflfs for livestock are attractive in terms of both speed and economy. The in vitro pressure transducer technique (PTT) whereby the gas evolved during the in vitro fermentation of feedstuffs in rumen fluid is quantified by a pressure transducer, has been used to predict the nutritive value of ruminant feedstuffs. Here the potential for the PTT in predicting the digestible energy contents of eight feedstuffs varying in non-starch polysaccharide (NSP) content fed to growing pigs, was investigated.

A comparison between in vitro digestibility, in situ degradability and a gas production technique for predicting the in vivo digestibility of whole-crop wheat

1998 ◽  
Vol 22 ◽  
pp. 33-35
Author(s):  
A. T. Adesogan ◽  
E. Owen ◽  
D. I. Givens
Keyword(s):  
Nutritive Value ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Production Technique ◽  
Grass Silage ◽  
Laboratory Techniques

Several published reports on the nutritive value of whole-crop wheat (WCW) have been based on estimations from laboratory techniques, some of which were developed for grass silage. However, there is little information on the accuracy of such estimations. Therefore the aim of this study was to evaluate the suitability of predicting the in vivo digestibility of WCW from various less animal-dependent techniques.

Relationship between in vitro gas production and in vivo energy digestibility in growing pigs

1996 ◽  
Vol 1996 ◽  
pp. 142-142
Author(s):  
A.C. Longland ◽  
S.P. Bray ◽  
A.E. Brooks ◽  
M.K. Theodorou ◽  
A.G. Low
Keyword(s):  
Pressure Transducer ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Growing Pigs ◽  
Digestible Energy ◽  
In Vitro Gas Production

In vitro methods which can be used to predict the nutritive value of feedstuflfs for livestock are attractive in terms of both speed and economy. The in vitro pressure transducer technique (PTT) whereby the gas evolved during the in vitro fermentation of feedstuffs in rumen fluid is quantified by a pressure transducer, has been used to predict the nutritive value of ruminant feedstuffs. Here the potential for the PTT in predicting the digestible energy contents of eight feedstuffs varying in non-starch polysaccharide (NSP) content fed to growing pigs, was investigated.

A Comparison between in vitro digestibility, in situ degradability and a gas production technique for predicting the in vivo digestibility of whole crop wheat

1995 ◽  
Vol 1995 ◽  
pp. 118-118
Author(s):  
A T Adesogan ◽  
D I Giverts ◽  
E Owen
Keyword(s):  
Nutritive Value ◽  
Animal Experimentation ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Production Technique ◽  
Non Invasive ◽  
Evaluation Techniques

Public aversion to animal experimentation demands the replacement of typically expensive, laborious and lengthy in vivo forage evaluation techniques with non-invasive, laboratory-based techniques. This study aimed to assess the suitability of three less animal-dependent techniques (in vitro digestibility (Tilley and Terry, 1963), in situ degradability (Ørskov and McDonald, 1979) and gas production (Theodorou et al., 1994)) for predicting the in vivo digestibility of whole crop wheat (WCW). The study forms part of an on-going MAFF LINK project that aims to develop equations for the laboratory-based prediction of the nutritive value of WCW.

A Comparison between in vitro digestibility, in situ degradability and a gas production technique for predicting the in vivo digestibility of whole crop wheat

1995 ◽  
Vol 1995 ◽  
pp. 118-118
Author(s):  
A T Adesogan ◽  
D I Giverts ◽  
E Owen
Keyword(s):  
Nutritive Value ◽  
Animal Experimentation ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Production Technique ◽  
Non Invasive ◽  
Evaluation Techniques

Public aversion to animal experimentation demands the replacement of typically expensive, laborious and lengthy in vivo forage evaluation techniques with non-invasive, laboratory-based techniques. This study aimed to assess the suitability of three less animal-dependent techniques (in vitro digestibility (Tilley and Terry, 1963), in situ degradability (Ørskov and McDonald, 1979) and gas production (Theodorou et al., 1994)) for predicting the in vivo digestibility of whole crop wheat (WCW). The study forms part of an on-going MAFF LINK project that aims to develop equations for the laboratory-based prediction of the nutritive value of WCW.

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