Transmission and Scanning Electron Microscopy of the Follicle in the Human Ovary

1974 ◽  
Vol 32 ◽  
pp. 142-143
Author(s):  
I. M. Baccarini
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Granulosa Cells ◽  
Osmium Tetroxide ◽  
Human Ovary ◽  
Scanning Microscope ◽  
Electron Microscopic ◽  
Ultrastructural Studies ◽  
Microscopic Studies ◽  
Scanning Electron

Several ultrastructural studies demonstrated that the granulosa cells changed the structure since their differentiation, maturation and degeneration. However, little is known about the organization of the granulosa cells in the follicle for instance, the connection between the cells and cellular interchanges.Fragments of ovary obtained from the surgery room were fixed in 3% buffered glutaraldehyde for scanning microscope and post-fixed in 1% buffered osmium tetroxide for electron microscopic studies.

Scanning Electron Microscopy of Murine Oncornavirus Infected Cells

1974 ◽  
Vol 32 ◽  
pp. 252-253 ◽  
Author(s):  
S. Panem ◽  
P.S.D. Lin ◽  
A. V. Crewe ◽  
W. H. Kirsten
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscopic ◽  
Viral Budding ◽  
Transmission Electron ◽  
Virion Structure ◽  
Microscopic Studies ◽  
Infected Cells ◽  
Viral Morphogenesis ◽  
Scanning Electron

Although oncornaviruses have been the subject of numerous transmission electron microscopic studies, questions concerning virion structure and the maturation of virus at the infected cell membrane remain unanswered. We have begun to study viral morphogenesis with particular emphasis on the distribution of viral budding sites and the appearance of virus during maturation using scanning electron microscopy.

Scanning Electron Microscopic Studies on the Tegument of Trematodes

1974 ◽  
Vol 32 ◽  
pp. 182-183
Author(s):  
J. E. Ubelaker ◽  
R. D. Specian ◽  
V. F. Allison
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Host Response ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Microscopic Studies ◽  
Physiological Demands ◽  
Electron Microscopes ◽  
Scanning Electron Microscopes ◽  
Scanning Electron

Among the parasitic flatworms, only members of the trematoda have exploited nearly every conceivable niche. Since physiological demands in each habitat present special problems in eluding the host response as well as obtaining nourishment the surface epithelia of such organisms warrant special attention. To gain an appreciation of tegumental diversity in the trematoda, representative trematodes from numerous habitats in their respective hosts were examined by scanning electron microscopySpecimens were collected from natural infections, fixed in paraformaldehyde and dehydrated in alcohol. Ethanol was exchanged with amyl acetate prior to CO2 drying in a Denton DCP-1 critical point dryer. The dried specimens were mounted on metal holders, outgassed and rotary coated with gold-palladium. These were then examined with the ISI Mini-SEM and AMR 1000 scanning electron microscopes.

Light and scanning electron microscopic studies on Trichodina labrisomi n.sp. from Labrisomas nuchipinnis (Osteichthyes: Labrisomidae) from Mangrove Lake, Bermuda

1993 ◽  
Vol 71 (9) ◽  
pp. 1855-1860
Author(s):  
Thomas G. Rand
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Body Shape ◽  
The Body ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Microscopic Studies ◽  
Oral Surface ◽  
Scanning Electron

Trichodina labrisomi n.sp. from the gills of the hairy blenny, Labrisomas nuchipinnis (Quoy and Gaimard), in Mangrove Lake, Bermuda, is described using light microscopy and scanning electron microscopy (SEM). Light microscopy reveals that the dimensions and morphology of T. labrisomi are similar to those given for only 13 previously described species of Trichodina (T. acuta Lom, 1961; T. baicalensis Dogiel, 1957; T. cottomephori Stein, 1979; T. dalli Zhukov, 1964; Trichodina domerguei domerguei Lom and Shtein 1966; T. elegans Stein, 1979; T. fultoni Davis, 1947; T. jadranica Haider, 1964; T. jadranica noblei Lom, 1970; T. partidisci Haider, 1964; T. reticulata Hirschmann et Partsch, 1955; T. tenuidens Faure-Fremiet, 1943; and T. tenuiformis Stein, 1979). However, compared with these similar forms, T. labrisomi n.sp. is distinguished by the dimensions of its body and denticulate ring and (or) the appearance of its silver-impregnated adhesive disc and denticles. Features of T. labrisomi examined by SEM include the body shape, pellicle, aboral pellicular pores, border membrane, aboral ciliary complex, and adoral ciliature, which are described and compared with those of other trichodinids studied using SEM. SEM also revealed that bacilli were distributed circumequatorially over the oral surface of T. labrisomi.

Light and scanning electron microscopic studies of spore formation in Ustilago pustulata and U. scabiosae

1981 ◽  
Vol 59 (2) ◽  
pp. 122-128 ◽  
Author(s):  
G. Deml ◽  
M. Nebel ◽  
F. Oberwinkler
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Spore Formation ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Microscopic Studies ◽  
Scanning Electron

The development of sori, sporogenesis, and characteristics of the hyphae in Ustilago pustulata and U. scabiosae have been investigated by light and scanning electron microscopy. The septa of both species form clamp connections. In U. scabiosae the sporogenous cells are comparable with budding mycelia. The teliospores of Ustilago pustulata and U. scabiosae are formed endogenously in chains.

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

1971 ◽  
Vol 29 ◽  
pp. 410-411 ◽  
Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Osmium Tetroxide ◽  
External Surface ◽  
Three Dimensional ◽  
Surface Structures ◽  
Transmission Microscopy ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

A New Image Processing Technique for STEM

1974 ◽  
Vol 32 ◽  
pp. 432-433
Author(s):  
Yasushi Kokubo ◽  
Hirotami Koike ◽  
Teruo Someya
Keyword(s):  
Electron Microscopy ◽  
Image Processing ◽  
Scanning Electron Microscopy ◽  
Elastic Scattering ◽  
Field Emission ◽  
Processing Technique ◽  
Image Processing Technique ◽  
Energy Analyzer ◽  
Scanning Microscope ◽  
Scanning Electron

One of the advantages of scanning electron microscopy is the capability for processing the image contrast, i.e., the image processing technique. Crewe et al were the first to apply this technique to a field emission scanning microscope and show images of individual atoms. They obtained a contrast which depended exclusively on the atomic numbers of specimen elements (Zcontrast), by displaying the images treated with the intensity ratio of elastically scattered to inelastically scattered electrons. The elastic scattering electrons were extracted by a solid detector and inelastic scattering electrons by an energy analyzer. We noted, however, that there is a possibility of the same contrast being obtained only by using an annular-type solid detector consisting of multiple concentric detector elements.

Electron Microscopic Observation of the Longitudinal Organization of Poly (p-Phenylene Terephthalamide) Fibers

1982 ◽  
Vol 40 ◽  
pp. 680-681
Author(s):  
Li Li-Sheng ◽  
L.F. Allard ◽  
W.C. Bigelow
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Aromatic Polyamides ◽  
Radial Arrangement ◽  
Scanning Electron ◽  
Better Than

The aromatic polyamides form a class of fibers having mechanical properties which are much better than those of aliphatic polyamides. Currently, the accepted morphology of these fibers as proposed by M.G. Dobb, et al. is a radial arrangement of pleated sheets, with the plane of the pleats parallel to the axis of the fiber. We have recently obtained evidence which supports a different morphology of this type of fiber, using ultramicrotomy and ion-thinning techniques to prepare specimens for transmission and scanning electron microscopy.

A Scanning Electron Microscopic Study of the Uriniferous Tubules

1973 ◽  
Vol 31 ◽  
pp. 622-623
Author(s):  
Peter M. Andrews
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Renal Glomerulus ◽  
Electron Microscopic ◽  
Vascular Perfusion ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Collecting Tubules ◽  
Bowman's Capsule ◽  
Scanning Electron

Although there have been a number of recent scanning electron microscopic reports on the renal glomerulus, the advantages of scanning electron microscopy have not yet been applied to a systematic study of the uriniferous tubules. In the present investigation, scanning electron microscopy was used to study the ultrastructural morphology of the proximal, distal, thin loop, and collecting tubules. Material for observation was taken from rat kidneys which were fixed by vascular perfusion, sectioned by either cutting or fracturing technigues, and critically point dried.The brush border characterising proximal tubules is first detected on the luminal surface of Bowman's capsule adjacent to the urinary pole orifice. In this region one frequently finds irregular microvilli characterized by broad and flattened bases with occasional bulbous structures protruding from their surfaces.

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