Ultrastructure of the Synovial Microvasculature in Rheumatoid Arthritis (ra) and Systemic Lupus Erythematosus (SLE)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068199 ◽

1970 ◽

Vol 28 ◽

pp. 240-241

Author(s):

H. Ralph Schumacher

Keyword(s):

Lupus Erythematosus ◽

Uranyl Acetate ◽

Basement Membranes ◽

Ultrastructural Changes ◽

Lead Citrate ◽

Vascular Changes ◽

Systemic Lupus ◽

Degenerative Arthritis ◽

Cacodylate Buffer ◽

Human Joint

Synovial vascular alterations have been suggested to be important in the pathogenesis of both RA and SLE (1). Although vascular changes have been described by light microscopy the presence of significant ultrastructural changes has been questioned (2). This report describes an EM study of needle synovial biopsies from 5 patients each with classical RA and SLE. Specimens were promptly fixed in Karnovsky's paraformaldehyde-glutaraldehyde diluted 1:1 with 0.1 M cacodylate buffer at pH 7.4, washed in buffer, post-fixed in Palade's osmium-veronal, dehydrated with alcohol, embedded in epon, cut, and stained with lead citrate and uranyl acetate. Vascular changes were seen in both groups with some findings common to both diseases. Basement membranes were multilaminated. (Fig.l). This was not seen in normal rabbits and monkeys but was also present in other human joint diseases including degenerative arthritis. Venular endothelium was active appearing with filopodia extending into the lumen and with gaps demonstrable between endothelial cells (Fig.2,3). Platelets occluded some gaps (Fig.2).

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Ultrastructural changes in sciatic nerve tissue: Effects of passive maternal smoking

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100076858 ◽

1983 ◽

Vol 41 ◽

pp. 650-651

Author(s):

Amankwah K.S. ◽

A.D. Weberg ◽

R.C. Kaufmann

Keyword(s):

Sciatic Nerve ◽

Maternal Smoking ◽

Ultrastructural Level ◽

Uranyl Acetate ◽

Nerve Tissue ◽

Ultrastructural Changes ◽

Cacodylate Buffer ◽

And Control ◽

Spontaneous Delivery

Previous research has revealed that passive (involuntary inhalation) tobacco smoking during gestation can have adverse effects upon the developing fetus. These prior investigations did not concentrate on changes in fetal morphology. This study was undertaken to delineate fetal neural abnormalities at the ultrastructural level in mice pups exposed in utero to passive maternal smoking.Pregnant study animals, housed in a special chamber, were subjected to cigarette smoke daily from conception until delivery. Blood tests for determination of carbon monoxide levels were run at 15-18 days gestation. Sciatic nerve tissue from experimental and control animals were obtained following spontaneous delivery and fixed in 2.5% gluteraldehyde in 0.1M cacodylate buffer pH 7.3. The samples were post-fixed in osmium ferrocyanide (1:1 mixture of 1.5% aqueous OSO4 and 2.5% K4 Fe(CN)6). Following dehydration, the tissues were infiltrated with and embedded in Spurr. Sections were stained with uranyl acetate and lead citrate.

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Fish kidney cell line in response to heat shock

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123921 ◽

1992 ◽

Vol 50 (1) ◽

pp. 704-705

Author(s):

Li-Chu Tung ◽

Yung-Reui Chen ◽

Shiu-Nan Chen ◽

Guang-Hsiung Kuo

Keyword(s):

Heat Shock ◽

Cell Line ◽

Fetal Calf Serum ◽

Calf Serum ◽

Uranyl Acetate ◽

Ultrastructural Changes ◽

Heat Shock Treatment ◽

Acanthopagrus Schlegeli ◽

Cacodylate Buffer ◽

Fish Kidney

In the present study, the ultrastructural changes of BPK cells, a fibroblast-like cell line, derived from the kidney of juvenile black porgy Acanthopagrus schlegeli, under heat shock treatment are described.The BPK cells were maintained in L-15 medium supplemented with 10% fetal calf serum and 0.15 M NaCl at 28|C2. The heating was carried out in precalibrated water baths. Monolayers of cells, grown on coverslips in parafilm-sealed petri dishes were submerged under water for 30 min at 40|C treatments. Cells were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer supplemented with 6.6% sucrose, postfixed in 1% OsO4 and flat embedded in Spurr’s resin. Silver section were cut parallel to the substratum, stained with uranyl acetate and Reynold’s lead citrate, and examined in a Hitachi H-600 electron microscope at 75 KV.

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Dextran Sulfate (Selesorb) Plasma Apheresis Improves Vascular Changes in Systemic Lupus Erythematosus

Therapeutic Apheresis ◽

10.1046/j.1526-0968.2002.00408.x ◽

2002 ◽

Vol 6 (6) ◽

pp. 471-477 ◽

Cited By ~ 14

Author(s):

Norbert Braun ◽

Michael Jünger ◽

Reinhild Klein ◽

Sylvia Gutenberger ◽

Michael Guagnin ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Dextran Sulfate ◽

Vascular Changes ◽

Systemic Lupus

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Gap junction associated filaments in testis interstitial cells of the rat

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010008362x ◽

1978 ◽

Vol 36 (2) ◽

pp. 550-551

Author(s):

J.F. David-Ferreira ◽

K.L. David-Ferreira ◽

M.H. Miranda ◽

J.C. Lemos

Keyword(s):

Gap Junction ◽

Freeze Fracture ◽

Interstitial Cells ◽

Uranyl Acetate ◽

Lead Citrate ◽

Thin Sections ◽

Cacodylate Buffer ◽

Old Rats ◽

The Relationship ◽

Made In

The testis interstitial cells of the rat are closely associated forming small aggregates in the vicinity of the vessels. The finality of the present study is to analise the relationship between the interstitial cells and to describe some particularities of their junctions.The observations were made in thin sections from testis of 2 to 3 months old rats fixed by immersion or perfusion with 2 to 3% glutaral- dehyde in s-collidine or cacodylate buffer followed by 2% osmium tetro-xyde in the same buffers. Some specimens have been prepared following the technique of Shea. The thin sections obtained after embedding in Epon were double stained in uranyl acetate and lead citrate. For the cryofracture study the tissues were fixed in 3% glutaraldehyde in cacodylate buffer, impregnated in 28% glycerol, frozen in Freon 22 and stored in liquid nitrogen. Freeze fracture and platinum-carbon shadowing were done in a Balzers 300.

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Connective Tissue Disorders in Otolaryngology

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348947408300306 ◽

1974 ◽

Vol 83 (3) ◽

pp. 314-322 ◽

Cited By ~ 6

Author(s):

Frank E. Lucente

Keyword(s):

Connective Tissue ◽

Lupus Erythematosus ◽

Respiratory Function ◽

Vascular Changes ◽

Connective Tissue Disorders ◽

Systemic Lupus ◽

Fatal Disease ◽

Temporomandibular Joints ◽

Life Threatening ◽

Cricoarytenoid Arthritis

Connective tissue disorders may present with various otolaryngologic symptoms. The manifestations may represent vasculopathy, arthopathy, neuropathy or a combination of pathological processes. Rheumatoid arthritis may involve the middle ear, temporomandibular joints and larynx with mild symptoms or may be life-threatening in instances of cricoarytenoid arthritis. Dermatomyositis with its cutaneous and muscular symptoms prevalent in the head and neck is frequently associated with malignancy in patients over 40. Scleroderma not only affects the gastrointestinal tract but can also produce significant limitations of respiratory function. Systemic lupus erythematosus, polyarteritis modosa, Wegener's granulomatosis and giant cell arteritis produce numerous vascular changes in structures of otolaryngologic significance. In this puzzling group of disorders, protean symptoms may precede the development of a fulminant, widespread and fatal disease.

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Association of Renal Resistive Index with Markers of Extrarenal Vascular Changes in Patients with Systemic Lupus Erythematosus

Ultrasound in Medicine & Biology ◽

10.1016/j.ultrasmedbio.2015.12.025 ◽

2016 ◽

Vol 42 (5) ◽

pp. 1103-1110 ◽

Cited By ~ 3

Author(s):

Massimiliano Morreale ◽

Giuseppe Mulè ◽

Angelo Ferrante ◽

Francesco D'ignoto ◽

Santina Cottone

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Resistive Index ◽

Renal Resistive Index ◽

Vascular Changes ◽

Systemic Lupus

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“Further Ultrastructure Observations of the Salamander Heart Maintained in Vitro”

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006012x ◽

1967 ◽

Vol 25 ◽

pp. 22-23

Author(s):

Edward W. Millhouse

Keyword(s):

Morphological Changes ◽

Uranyl Acetate ◽

Culture Period ◽

Lead Citrate ◽

Initial Report ◽

Taricha Torosa ◽

Cacodylate Buffer

Three years ago an initial report from our laboratory demonstrated the morphological changes observed in whole larval salamander (Taricha torosa) hearts maintained in culture for six months. Since then we have examined whole hearts that have been cultured from one to four months. During the culture period all hearts continued and maintained a particular beat with fluctuations in pulsation rates indicating a 24-hour periodicity.This report will deal with the ultrastructure observations of control hearts and one, two, three, and six month cultured hearts. All tissues were fixed initially from 4 to 6 hours at 4°C in 4%, redistilled glutaraldehyde buffered with cacodylate to a pH 7.4. Then the atrium was dissected free and the ventricle cut in half and, using fresh fixative, the fixation was continued for 10 hours. The tissues were washed in cacodylate buffer and stored in the cacodylate buffer containing 7% sucrose for 12 to 24 hours. These tissues were washed in buffer and post-fixed in 2% OsO4 buffered with cacodylate to pH 7.4 for two hours at 4°C. Then the tissues were dehydrated in graded ethanols, embedded in Epon 812, and sectioned on a Porter-Blum ultramicrotome. Sections were stained for 30 minutes in saturated aqueous uranyl acetate and for 15 minutes in lead citrate and viewed in an RCA EMU-3H.

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Ultrastructural Characteristics of Vaginal Epithelium of Persistent Estrous Rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119983 ◽

1985 ◽

Vol 43 ◽

pp. 658-659

Author(s):

Khosho Francis K. ◽

Kaufmann Robert C. ◽

Amankwah Kofi S.

Keyword(s):

Osmium Tetroxide ◽

Uranyl Acetate ◽

Ruthenium Red ◽

Female Rats ◽

Constant Light ◽

Ultrastructural Changes ◽

Vaginal Smears ◽

Thin Sections ◽

Cacodylate Buffer ◽

Ultrastructural Characteristics

Adult female rats exposed to constant light will develop anovulatory acyclicity characterized by persistent vaginal cornification (PE) and formation of multiple large cystic follicles on the ovaries. The purpose of the present communication is to describe the ultrastructural changes in vaginal epithelia in PE rats as compared to that in normal estrous rats.Persistent vaginal estrous with PCO was induced in a group of Sprague-Dawely rats by exposure to constant light for 50-150 days. Rats in normal estrous, as determined by vaginal smears, were used as controls. Nembutal- anethesized rats were perfused through the aorta with 2.5% gluteraldehyde in 1M sodium cacodylate buffer (pH 7.3). The mucosa of the vaginal folds just inferior to the cervix were dissected by microsurgery, postfixed, stained with 0.5% ruthenium red in 1% osmium tetroxide, dehydrated, and embedded in polybed. Thick sections (1μ) were stained with toludine blue for light microscopy studies. Thin sections were stained with uranyl acetate and lead citrate.

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Bacterioids in the Rectal Organ of the Lantern Dug Pyrops Candelaria Linn (Homoptera: Fulgoridae)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010016649x ◽

1996 ◽

Vol 54 ◽

pp. 806-807

Author(s):

W.W.K. Cheung ◽

J.B. Wang

Keyword(s):

Fine Structure ◽

Electron Microscope ◽

Osmium Tetroxide ◽

Uranyl Acetate ◽

Sodium Cacodylate Buffer ◽

Lead Citrate ◽

Sodium Cacodylate ◽

Special Pair ◽

Adult Females ◽

Cacodylate Buffer

The lantern bug harbours three symbionts, namely a, x and i in its body. These microorganisms are supposed to be transmitted transovarially to the future progeny. The x-symbionts are found in a special pair of organs called the x-organ which bulges to form a rectal organ in adult females. The purpose of this study is to investigate into the fine structure of the x-symbionts. This will serve as a basis for understanding the interactions of this microorganism with its host.The rectum of the lantern bug Pyrops candelaria Linn, was dissected out in buffered insect saline and fixed in 2.5% glutaradehyde in 0.1M sodium cacodylate buffer (pH 7.2) for 1 hr. The rectal organ was subsequently post-fixed in 1% osmium tetroxide (pH 7.2) and dehydrated in alcohol/acetone series. These were blocked in Spurr resin and cut with a Reichert Ultratome. Sections were stained with uranyl acetate and lead citrate and examined with a JEOL JEM-1200EX electron microscope. Thick sections (1 μn) were stained with 1% toluidine blue and examined under a Nikon Optiphot light microscope.

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