Ultrastructural changes in sciatic nerve tissue: Effects of passive maternal smoking

1983 ◽  
Vol 41 ◽  
pp. 650-651
Author(s):  
Amankwah K.S. ◽  
A.D. Weberg ◽  
R.C. Kaufmann
Keyword(s):  
Sciatic Nerve ◽  
Maternal Smoking ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Nerve Tissue ◽  
Ultrastructural Changes ◽  
Cacodylate Buffer ◽  
And Control ◽  
Spontaneous Delivery

Previous research has revealed that passive (involuntary inhalation) tobacco smoking during gestation can have adverse effects upon the developing fetus. These prior investigations did not concentrate on changes in fetal morphology. This study was undertaken to delineate fetal neural abnormalities at the ultrastructural level in mice pups exposed in utero to passive maternal smoking.Pregnant study animals, housed in a special chamber, were subjected to cigarette smoke daily from conception until delivery. Blood tests for determination of carbon monoxide levels were run at 15-18 days gestation. Sciatic nerve tissue from experimental and control animals were obtained following spontaneous delivery and fixed in 2.5% gluteraldehyde in 0.1M cacodylate buffer pH 7.3. The samples were post-fixed in osmium ferrocyanide (1:1 mixture of 1.5% aqueous OSO4 and 2.5% K4 Fe(CN)6). Following dehydration, the tissues were infiltrated with and embedded in Spurr. Sections were stained with uranyl acetate and lead citrate.

Fish kidney cell line in response to heat shock

1992 ◽  
Vol 50 (1) ◽  
pp. 704-705
Author(s):  
Li-Chu Tung ◽  
Yung-Reui Chen ◽  
Shiu-Nan Chen ◽  
Guang-Hsiung Kuo
Keyword(s):  
Heat Shock ◽  
Cell Line ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Uranyl Acetate ◽  
Ultrastructural Changes ◽  
Heat Shock Treatment ◽  
Acanthopagrus Schlegeli ◽  
Cacodylate Buffer ◽  
Fish Kidney

In the present study, the ultrastructural changes of BPK cells, a fibroblast-like cell line, derived from the kidney of juvenile black porgy Acanthopagrus schlegeli, under heat shock treatment are described.The BPK cells were maintained in L-15 medium supplemented with 10% fetal calf serum and 0.15 M NaCl at 28|C2. The heating was carried out in precalibrated water baths. Monolayers of cells, grown on coverslips in parafilm-sealed petri dishes were submerged under water for 30 min at 40|C treatments. Cells were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer supplemented with 6.6% sucrose, postfixed in 1% OsO4 and flat embedded in Spurr’s resin. Silver section were cut parallel to the substratum, stained with uranyl acetate and Reynold’s lead citrate, and examined in a Hitachi H-600 electron microscope at 75 KV.

Ultrastructure of the Synovial Microvasculature in Rheumatoid Arthritis (ra) and Systemic Lupus Erythematosus (SLE)

1970 ◽  
Vol 28 ◽  
pp. 240-241
Author(s):  
H. Ralph Schumacher
Keyword(s):  
Lupus Erythematosus ◽  
Uranyl Acetate ◽  
Basement Membranes ◽  
Ultrastructural Changes ◽  
Lead Citrate ◽  
Vascular Changes ◽  
Systemic Lupus ◽  
Degenerative Arthritis ◽  
Cacodylate Buffer ◽  
Human Joint

Synovial vascular alterations have been suggested to be important in the pathogenesis of both RA and SLE (1). Although vascular changes have been described by light microscopy the presence of significant ultrastructural changes has been questioned (2). This report describes an EM study of needle synovial biopsies from 5 patients each with classical RA and SLE. Specimens were promptly fixed in Karnovsky's paraformaldehyde-glutaraldehyde diluted 1:1 with 0.1 M cacodylate buffer at pH 7.4, washed in buffer, post-fixed in Palade's osmium-veronal, dehydrated with alcohol, embedded in epon, cut, and stained with lead citrate and uranyl acetate. Vascular changes were seen in both groups with some findings common to both diseases. Basement membranes were multilaminated. (Fig.l). This was not seen in normal rabbits and monkeys but was also present in other human joint diseases including degenerative arthritis. Venular endothelium was active appearing with filopodia extending into the lumen and with gaps demonstrable between endothelial cells (Fig.2,3). Platelets occluded some gaps (Fig.2).

Ultrastructural Characteristics of Vaginal Epithelium of Persistent Estrous Rats

1985 ◽  
Vol 43 ◽  
pp. 658-659
Author(s):  
Khosho Francis K. ◽  
Kaufmann Robert C. ◽  
Amankwah Kofi S.
Keyword(s):  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Ruthenium Red ◽  
Female Rats ◽  
Constant Light ◽  
Ultrastructural Changes ◽  
Vaginal Smears ◽  
Thin Sections ◽  
Cacodylate Buffer ◽  
Ultrastructural Characteristics

Adult female rats exposed to constant light will develop anovulatory acyclicity characterized by persistent vaginal cornification (PE) and formation of multiple large cystic follicles on the ovaries. The purpose of the present communication is to describe the ultrastructural changes in vaginal epithelia in PE rats as compared to that in normal estrous rats.Persistent vaginal estrous with PCO was induced in a group of Sprague-Dawely rats by exposure to constant light for 50-150 days. Rats in normal estrous, as determined by vaginal smears, were used as controls. Nembutal- anethesized rats were perfused through the aorta with 2.5% gluteraldehyde in 1M sodium cacodylate buffer (pH 7.3). The mucosa of the vaginal folds just inferior to the cervix were dissected by microsurgery, postfixed, stained with 0.5% ruthenium red in 1% osmium tetroxide, dehydrated, and embedded in polybed. Thick sections (1μ) were stained with toludine blue for light microscopy studies. Thin sections were stained with uranyl acetate and lead citrate.

Fine structure of human sperm entry into zona-free hamster oocyte

1978 ◽  
Vol 36 (2) ◽  
pp. 540-541
Author(s):  
C. Barros ◽  
J. González ◽  
E. Herrera ◽  
E. Bustos-Obregón
Keyword(s):  
Osmium Tetroxide ◽  
Human Sperm ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Human Spermatozoa ◽  
Low Viscosity ◽  
Cacodylate Buffer ◽  
Ultrathin Sections ◽  
Hamster Oocyte

Zona-free mammalian oocytes have been observed to fuse, under in vitro conditions, With non-homologous spermatozoa. Taking advantage of this heterologous gamete fusion, we designed a bioassay to test -by means of zona-free hamster oocytes- the fertile ability of human spermatozoa, from semen samples of patients attending an Infertility Clinic. To further validate our bioassay, which Was reported elsewhere, we studied the behavior of gamete membranes during fusion at the ultrastructural level.Zona-free hamster oocytes were mixed in vitro with human spermatozoa. At different times after the start of incubation, oocytes were fixed in 1% glutaraldehyde in 0. 25M cacodylate buffer pH 7. A and post-fixed in 196 osmium tetroxide. After dehydration in acetone, they were embedded in a low viscosity epoxy resin. Ultrathin sections were stained with uranyl acetate and lead citrate and examined with a Phillips 300 electron microscope.

Ultrastructural Changes of the Symbiotic Chlorella-Like Alga Isolated from Hydra Viridis

1982 ◽  
Vol 40 ◽  
pp. 320-321
Author(s):  
K.W. Lee ◽  
R.H. Meints ◽  
D. Kuczmarski ◽  
J.L. Van Etten
Keyword(s):  
Time Course ◽  
Reciprocal Cross ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Symbiotic Relationship ◽  
Cell Recognition ◽  
Green Hydra ◽  
Different Strains ◽  
Hydra Viridis

The physiological, biochemical, and ultrastructural aspects of the symbiotic relationship between the Chlorella-like algae and the hydra have been intensively investigated. Reciprocal cross-transfer of the Chlorellalike algae between different strains of green hydra provide a system for the study of cell recognition. However, our attempts to culture the algae free of the host hydra of the Florida strain, Hydra viridis, have been consistently unsuccessful. We were, therefore, prompted to examine the isolated algae at the ultrastructural level on a time course.

Effects of renovascular hypertension on rat adrenal zona glomerulosa

1978 ◽  
Vol 36 (2) ◽  
pp. 582-583
Author(s):  
G. Mazzocchi ◽  
P. Rebuffat ◽  
C. Robba ◽  
P. Vassanelli ◽  
G. G. Nussdorfer
Keyword(s):  
Renovascular Hypertension ◽  
Left Kidney ◽  
Renin Angiotensin System ◽  
Plasma Renin ◽  
Zona Glomerulosa ◽  
Ultrastructural Changes ◽  
Albino Rats ◽  
Left Renal Artery ◽  
Angiotensin System ◽  
And Control

It is well known that the rat adrenal zona glomerulosa steroidogenic activity is controlled by the renin-angiotensin system. The ultrastructural changes in the rat zona glomerulosa cells induced by renovascular hypertension were described previously, but as far as we are aware no correlated biochemical and morphometric investigations were performed.Twenty adult male albino rats were divided into 2 experimental groups. One group was subjected to restriction of blood flow to the left kidney by the application of a silver clip about the left renal artery. The other group was sham-operated and served as a control. Renovascular hypertension developed in about 10 days: sistolic blood pressure averaged 165 ± 6. 4 mmHg, whereas it was about 110 ± 3. 8 mmHg in the control animals. The hypertensive and control rats were sacrificed 20 days after the operation. The blood was collected and plasma renin activity was determined by radioimmunological methods. The aldosterone concentration was radioimmunologically assayed both in the plasma and in the homogenate of the left capsular adrenal gland.

The Nature of Rapidly Extracted Phycocyanin from the Blue-Green Alga Plectonema Boryanum

1971 ◽  
Vol 29 ◽  
pp. 366-367
Author(s):  
M. Kessel ◽  
R. MacColl
Keyword(s):  
Self Assembly ◽  
Analytical Ultracentrifugation ◽  
Uranyl Acetate ◽  
The Self ◽  
Major Protein ◽  
Subunit Structure ◽  
Blue Green Alga ◽  
Thin Sections ◽  
Blue Green Algae ◽  
Cacodylate Buffer

The major protein of the blue-green algae is the biliprotein, C-phycocyanin (Amax = 620 nm), which is presumed to exist in the cell in the form of distinct aggregates called phycobilisomes. The self-assembly of C-phycocyanin from monomer to hexamer has been extensively studied, but the proposed next step in the assembly of a phycobilisome, the formation of 19s subunits, is completely unknown. We have used electron microscopy and analytical ultracentrifugation in combination with a method for rapid and gentle extraction of phycocyanin to study its subunit structure and assembly.To establish the existence of phycobilisomes, cells of P. boryanum in the log phase of growth, growing at a light intensity of 200 foot candles, were fixed in 2% glutaraldehyde in 0.1M cacodylate buffer, pH 7.0, for 3 hours at 4°C. The cells were post-fixed in 1% OsO4 in the same buffer overnight. Material was stained for 1 hour in uranyl acetate (1%), dehydrated and embedded in araldite and examined in thin sections.

The Ultrastructure of Metastatic Human Mammary Carcinoma After Prolonged Estrogen Therapy

1967 ◽  
Vol 25 ◽  
pp. 180-181
Author(s):  
John H.L. Watson ◽  
John L. Swedo ◽  
R.W. Talley
Keyword(s):  
Mammary Carcinoma ◽  
Osmium Tetroxide ◽  
Estrogen Therapy ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Human Mammary Carcinoma ◽  
Surgical Biopsy ◽  
Lead Hydroxide ◽  
Preliminary Study ◽  
Hormonal Therapies

A preliminary study of human mammary carcinoma on the ultrastructural level is reported for a metastatic, subcutaneous nodule, obtained as a surgical biopsy. The patient's tumor had responded favorably to a series of hormonal therapies, including androgens, estrogens, progestins, and corticoids for recurring nodules over eight years. The pertinent nodule was removed from the region of the gluteal maximus, two weeks following stilbestrol therapy. It was about 1.5 cms in diameter, and was located within the dermis. Pieces from it were fixed immediately in cold fixatives: phosphate buffered osmium tetroxide, glutaraldehyde, and paraformaldehyde. Embedment in each case was in Vestopal W. Contrasting was done with combinations of uranyl acetate and lead hydroxide.

Morphogenesis of a New Human Herpes-Type Virus Isolate (Sasha Virus)

1973 ◽  
Vol 31 ◽  
pp. 592-593
Author(s):  
R. F. Zeigel ◽  
W. Munyon
Keyword(s):  
Virus Isolate ◽  
Calf Serum ◽  
Uranyl Acetate ◽  
Human Herpes ◽  
Human Embryonic Lung ◽  
Human Herpes Virus ◽  
Intracytoplasmic Inclusions ◽  
Hel Cells ◽  
And Control

In continuing studies on the role of viruses in biochemical transformation, Dr. Munyon has succeeded in isolating a highly infectious human herpes virus. Fluids of buccal pustular lesions from Sasha Munyon (10 mo. old) uiere introduced into monolayer sheets of human embryonic lung (HEL) cell cultures propagated in Eagles’ medium containing 5% calf serum. After 18 hours the cells exhibited a dramatic C.P.E. (intranuclear vacuoles, peripheral patching of chromatin, intracytoplasmic inclusions). Control HEL cells failed to reflect similar changes. Infected and control HEL cells were scraped from plastic flasks at 18 hrs. of incubation and centrifuged at 1200 × g for 15 min. Resultant cell packs uiere fixed in Dalton's chrome osmium, and post-fixed in aqueous uranyl acetate. Figure 1 illustrates typical hexagonal herpes-type nucleocapsids within the intranuclear virogenic regions. The nucleocapsids are approximately 100 nm in diameter. Nuclear membrane “translocation” (budding) uias observed.

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