The consolidation of microscopy technology into a university research support facility: Reflections on the 1980's and projections for the 1990's at the university of iowa

1993 ◽  
Vol 51 ◽  
pp. 476-477
Author(s):  
Kenneth C. Moore
Keyword(s):  
Laser Scanning ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Research Support ◽  
University Of Iowa ◽  
Staff Members ◽  
The Past ◽  
Research Grade ◽  
The University ◽  
Support Facility

The University of Iowa Central Electron Microscopy Research Facility(CEMRF) was established in 1981 to support all faculty, staff and students needing this technology. Initially the CEMRF was operated with one TEM, one SEM, three staff members and supported about 30 projects a year. During the past twelve years, the facility has replaced all instrumentation pre-dating 1981, and now includes 2 TEM's, 2 SEM's, 2 EDS systems, cryo-transfer specimen holders for both TEM and SEM, 2 parafin microtomes, 4 ultamicrotomes including cryoultramicrotomy, a Laser Scanning Confocal microscope, a research grade light microscope, an Ion Mill, film and print processing equipment, a rapid cryo-freezer, freeze substitution apparatus, a freeze-fracture/etching system, vacuum evaporators, sputter coaters, a plasma asher, and is currently evaluating scanning probe microscopes for acquisition. The facility presently consists of 10 staff members and supports over 150 projects annually from 44 departments in 5 Colleges and 10 industrial laboratories. One of the unique strengths of the CEMRF is that both Biomedical and Physical scientists use the facility.

scholarly journals HUME Lab: výzkumná infrastruktura pro podporu experimentálního výzkumu v sociálních a humanitních vědách

E-psychologie ◽  
2021 ◽  
Vol 15 (4) ◽  
pp. 98-100
Author(s):  
Eva Kundtová Klucová
Keyword(s):  
Social Sciences ◽  
International Research ◽  
Research Infrastructure ◽  
Research Teams ◽  
The Past ◽  
Laboratory Services ◽  
Humanities And Social Sciences ◽  
The University ◽  
Support Facility

HUME Lab is a research infrastructure at the Faculty of Arts of Masaryk University University (FF MU). As a support facility, it helps with the implementation of experiment methodology within research in the humanities and social sciences. The laboratory services are available primarily to researchers from FF MU, but they are also open for any interested researchers across the university and beyond. Various projects using the HUME Lab equipment and services have been carried out in the past involving, for example, CEITEC, BUT, or various international research teams usually with the participation of FF MU researchers.

Quick-Freezing to Catch the Membrane Changes that Occur During Exocytosis

1977 ◽  
Vol 35 ◽  
pp. 676-679
Author(s):  
J.E. Heuser
Keyword(s):  
Synaptic Vesicle ◽  
Structural Changes ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Frog Neuromuscular Junction ◽  
The Past ◽  
Synaptic Vesicle Exocytosis ◽  
Quick Freezing ◽  
Vesicle Exocytosis ◽  
Membrane Changes

The technique that we have used to capture synaptic vesicle exocytosis at the frog neuromuscular junction - that of quick-freezing muscles followed by freeze fracture (3) or freeze substitution (6) - works sufficiently well now that it may be useful in other sorts of membrane studies, or studies of fast structural changes with the electron microscope. This note reviews the quickfreezing technique we use, and describes its application to the problem of synaptic vesicle exocytosis and recycling at the synapse.Here, many of the membrane changes of interest occur during the brief delay in synaptic transmission, on a time scale of milliseconds or fractions of milliseconds, and leave only traces thereafter. In the past, we have studied these left-over traces in tissues fixed with the standard chemicals for electron microscopy (1), and have inferred from them that vesicles discharge the quanta of neurotransmitters, as the physiologists would predict.

Formal and Informal Microscopy Education at the University of Iowa Central Microscopy Research Facility: Project Centered Training

2001 ◽  
Vol 7 (S2) ◽  
pp. 814-815
Author(s):  
Randy Nessler ◽  
Ryan Potter ◽  
Jodi Stahl ◽  
Katina Wilson ◽  
Thomas Moninger ◽  
...  
Keyword(s):  
Freeze Fracture ◽  
Research Facility ◽  
University Of Iowa ◽  
Technological Advances ◽  
Transmission Electron ◽  
Mössbauer Spectrometer ◽  
History Of ◽  
Electron Microscopes ◽  
Scanning Electron Microscopes ◽  
The University

The University of Iowa Central Microscopy Research Facility (CMRF) has been in existence for 27 years. Starting out as a transmission electron microscopy (TEM) research laboratory, the facility has offered formal college courses. These courses require that students identify a project to investigate during the semester. Theory from the formal lecture is reinforced by work performed in the laboratory session. From its modest beginnings, the CMRF has continually grown. Currently, the facility offers two Confocal microscopes, two Scanning Electron Microscopes, a Scanning Probe Microscope, Energy Dispersive Spectroscopy, a Mossbauer spectrometer, a PTI Ion Imaging/Ratio system, a Freeze Fracture apparatus, and three light microscopes equipped with CCD cameras. Techniques range from routine histology to in-situ hybridization. Technological advances over the history of the facility have not been confined to the lab. in the past, most lectures were given using overheads and 35mm slides.

Southeast Asia in Australasian Universities

1971 ◽  
Vol 2 (1) ◽  
pp. 78-85
Author(s):  
Nicholas Tarling
Keyword(s):  
Southeast Asia ◽  
Second World War ◽  
World War ◽  
Undergraduate Level ◽  
Staff Members ◽  
The Past ◽  
Graduate Research ◽  
Research Students ◽  
The University ◽  
Second World

Australasian universities are tied to Southeast Asia in a number of ways. The most obvious is through their teaching. Since the second world war, but more particularly in the past ten or fifteen years, this has paid more attention to Southeast Asia, its peoples, their history and their languages, than at any time since the first university institutions were founded in Australia and New Zealand in the nineteenth century. There are other, perhaps less obvious, ties. The teaching has been primarily at undergraduate level, but the staff members involved have, of course, been involved in research in the area. Possibly the majority would have been trained overseas, if not themselves of overseas origin; but quite recently, and especially at one or two centres, the number of graduate research students has become significant, at least in Australia. Some of the research has been published in book or monograph form by existing Australasian or overseas publishers, or by the university presses, whose numbers have augmented over the past decade.

A University-Based Model for Evaluating Inventions

2003 ◽  
Vol 4 (4) ◽  
pp. 225-235 ◽  
Author(s):  
Cynthia Wagner Weick
Keyword(s):  
New Product Development ◽  
Early Stage ◽  
Development Models ◽  
Staff Members ◽  
The Past ◽  
Graduate Research ◽  
The Pacific ◽  
The University ◽  
Research Assistants ◽  
Similar Programme

There is a need for programmes that provide independent inventors with balanced, data-based evaluations of their early-stage ideas. These inventors do not have access to the evaluation processes used in many companies to assess whether or not an idea warrants further investment of time and resources. In addition, invention promotion services that focus on independent inventors are often costly; and some of these have been accused of fraud. The business school at the University of the Pacific (UOP) launched a pilot invention evaluation service (IES) in autumn 1999. The process used in the IES is based on new product development models used in companies, and addresses basic market, technical and financial potential. The programme benefits not only the community of inventors: because IES staff members are graduate research assistants, it also provides a unique experiential learning opportunity for students. This article details the design of the service and the manner in which it has been implemented at UOP. Data from surveys of the inventors who have been served over the past three years is presented, which indicates that they have found the IES to be very effective in improving their decision making. Other universities may benefit from establishing a similar programme.

Creating Academic Web Space For Faculty: Research And Teaching Initiatives At The University of Iowa Libraries

2020 ◽  
Author(s):  
Carol Ann Hughes ◽  
Paul Soderdahl ◽  
Karen Zimmerman
Keyword(s):  
Web Sites ◽  
Academic Staff ◽  
Teaching Staff ◽  
Border Crossings ◽  
Home Pages ◽  
University Of Iowa ◽  
Web Based ◽  
Staff Members ◽  
Web Space ◽  
The University

The University of Iowa has several projects that are reshaping options for teaching staff and librarians as they work to build new types of academic resources. Two of these are Bailiwick and TWIST. Bailiwick is a web space where academic passions are realised in HTML and creative home pages. Bailliwick is home to Web sites that are experimental in form, like Border crossings, which provides comprehensive and in-depth resources, or that take on a narrow, highly specialised topic like French Feminists. In the Teaching with Innovative Style and Technology Project (TWIST), teaching staff are paired with librarians partners to create Web-based learning environments. These partners are called TWISTed Pairs. This semester 27 academic staff members from 13 departments are paired with 11 librarians from various departments, creating 35 course-related Web sites.

Novae in the Magellanic Clouds

1979 ◽  
Vol 46 ◽  
pp. 96-101
Author(s):  
J.A. Graham
Keyword(s):  
Large Magellanic Cloud ◽  
Magellanic Cloud ◽  
Magellanic Clouds ◽  
University Of Michigan ◽  
The Past ◽  
Spectroscopic Information ◽  
Objective Prism ◽  
The University ◽  
Small Cloud ◽  
Low Dispersion

During the past several years, a systematic search for novae in the Magellanic Clouds has been carried out at Cerro Tololo Inter-American Observatory. The Curtis Schmidt telescope, on loan to CTIO from the University of Michigan is used to obtain plates every two weeks during the observing season. An objective prism is used on the telescope. This provides additional low-dispersion spectroscopic information when a nova is discovered. The plates cover an area of 5°x5°. One plate is sufficient to cover the Small Magellanic Cloud and four are taken of the Large Magellanic Cloud with an overlap so that the central bar is included on each plate. The methods used in the search have been described by Graham and Araya (1971). In the CTIO survey, 8 novae have been discovered in the Large Cloud but none in the Small Cloud. The survey was not carried out in 1974 or 1976. During 1974, one nova was discovered in the Small Cloud by MacConnell and Sanduleak (1974).

Ca-Histochemistry in slime mold plasmodia

1978 ◽  
Vol 36 (2) ◽  
pp. 130-131
Author(s):  
Ulrich Dierkes
Keyword(s):  
Physarum Polycephalum ◽  
Carbon Coating ◽  
Freeze Drying ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Uranyl Acetate ◽  
Slime Mold ◽  
Staining Procedure ◽  
Protoplasmic Streaming ◽  
Intracellular Ca

Calcium is supposed to play an important role in the control of protoplasmic streaming in slime mold plasmodia. The motive force for protoplasmic streaming is generated by the interaction of actin and myosin. This contraction is supposed to be controlled by intracellular Ca-fluxes similar to the triggering system in skeleton muscle. The histochemical localisation of calcium however is problematic because of the possible diffusion artifacts especially in aquous media.To evaluate this problem calcium localisation was studied in small pieces of shock frozen (liquid propane at -189°C) plasmodial strands of Physarum polycephalum, which were further processed with 3 different methods: 1) freeze substitution in ethanol at -75°C, staining in 100% ethanol with 1% uranyl acetate, and embedding in styrene-methacrylate. For comparison the staining procedure was omitted in some preparations. 2)Freeze drying at about -95°C, followed by immersion with 100% ethanol containing 1% uranyl acetate, and embedding. 3) Freeze fracture, carbon coating and SEM investigation at temperatures below -100° C.

Techniques for cryoultramicrotomy of propane jet frozen biological samples

1986 ◽  
Vol 44 ◽  
pp. 260-261
Author(s):  
B. Craig ◽  
L. Hawkey ◽  
A. LeFurgey
Keyword(s):  
Freeze Fracture ◽  
Freeze Substitution ◽  
Heart Cells ◽  
Crystal Formation ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Chick Heart ◽  
Embryonic Chick Heart ◽  
A New Technique

Ultra-rapid freezing followed by cryoultramicrotomy is essential for the preservation of diffusible elements in situ within cells prior to scanning transmission electron microscopy and quantitative energy dispersive x-ray microanalysis. For cells or tissue fragments in suspension and for monolayer cell cultures, propane jet freezing provides cooling rates greater than 30,000°C/sec with regions up to 40μm in thickness free of significant ice crystal formation. While this method of freezing has frequently been applied prior to freeze fracture or freeze substitution, it has not been widely utilized prior to cryoultramicrotomy and subsequent x-ray microanalytical studies. This report describes methods devised in our laboratory for cryosectioning of propane jet frozen kidney proximal tubule suspensions and cultured embryonic chick heart cells, in particular a new technique for mounting frozen suspension specimens for sectioning. The techniques utilize the same specimen supports and sample holders as those used for freeze fracture and freeze substitution and should be generally applicable to any cell suspension or culture preparation.

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