Immunoelectron microscopic localization of elastic tissue in archival material using an improved method

Elastic tissue is an important component of the walls of arteries and veins, of skin, of the lungs and in lesser amounts, of many other tissues. It is responsible for the rubber-like properties of the arteries and for the normal texture of young skin. It undergoes changes in a number of important diseases such as atherosclerosis and emphysema and on exposure of skin to sunlight.We have recently described methods for the localizationof elastic tissue components in normal animal and human tissues. In the study of developing and diseased tissues it is often not possible to obtain samples which have been optimally prepared for immuno-electron microscopy. Sometimes there is also a need to examine retrospectively samples collected some years previously. We have therefore developed modifications to our published methods to allow examination of human and animal tissue samples obtained at surgery or during post mortem which have subsequently been: 1. stored frozen at -35° or -70°C for biochemical examination; 2.

Download Full-text

Immunoelectron microscopic localization of elastic tissue components in archival tissue samples

Journal of Microscopy ◽

10.1111/j.1365-2818.1991.tb03146.x ◽

1991 ◽

Vol 162 (3) ◽

pp. 355-367 ◽

Cited By ~ 2

Author(s):

Joseph C. Fanning ◽

Jacinta F. White ◽

Roman Polewski ◽

Edward G. Cleary

Keyword(s):

Elastic Tissue ◽

Tissue Samples ◽

Archival Tissue ◽

Microscopic Localization

Download Full-text

Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot

Biology ◽

10.3390/biology9030059 ◽

2020 ◽

Vol 9 (3) ◽

pp. 59 ◽

Cited By ~ 2

Author(s):

Magali Humbert ◽

María Morán ◽

Patricia de la Cruz-Ojeda ◽

Jordi Muntané ◽

Tabea Wiedmer ◽

...

Keyword(s):

Human Tissue ◽

Degradation Mechanism ◽

Autophagic Flux ◽

Human Tissues ◽

Tissue Analysis ◽

Therapeutic Approaches ◽

Tissue Samples ◽

Clear Cut ◽

Autophagic Activity ◽

Made In

Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine autophagic activity in human tissues, an endeavor that is hampered by the fact that autophagy is characterized by the flux of substrates whereas histology informs only about amounts and localization of substrates and regulators at a single timepoint. Despite this challenging task, considerable progress in establishing markers of autophagy has been made in recent years. The importance of establishing clear-cut autophagy markers that can be used for tissue analysis cannot be underestimated. In this review, we attempt to summarize known techniques to quantify autophagy in human tissue and their drawbacks. Furthermore, we provide some recommendations that should be taken into consideration to improve the reliability and the interpretation of autophagy biomarkers in human tissue samples.

Download Full-text

Analytical procedure for mapping the distribution of 10B and 99Tc markers in cryo-sections of animal tissue samples by secondary ion mass spectrometry

Spectrochimica Acta Part B Atomic Spectroscopy ◽

10.1016/j.sab.2009.07.029 ◽

2009 ◽

Vol 64 (9) ◽

pp. 911-920 ◽

Cited By ~ 4

Author(s):

Ilaria Marchetti ◽

Luca Menichetti ◽

Claudia Kusmic ◽

Laura Aldave de las Heras ◽

Piero Salvadori ◽

...

Keyword(s):

Mass Spectrometry ◽

Analytical Procedure ◽

Secondary Ion Mass Spectrometry ◽

Animal Tissue ◽

Tissue Samples ◽

Ion Mass Spectrometry ◽

Secondary Ion

Download Full-text

Pathological, molecular and phylogenic study of fowlpox virus in domesticated chickens of Tikrit City, Iraq

Brazilian Journal of Veterinary Research and Animal Science ◽

10.11606/issn.1678-4456.bjvras.2021.176255 ◽

2021 ◽

Vol 58 ◽

pp. e176255

Author(s):

Ismael Ibrahim Hasan ◽

Saad Tawfik Rasheed ◽

Mohammed Khorshid Shakor

Keyword(s):

Phylogenetic Analysis ◽

Sequence Analysis ◽

Core Protein ◽

Chorioallantoic Membrane ◽

Genetic Distances ◽

Animal Tissue ◽

Economic Losses ◽

Cutaneous Lesions ◽

Fowlpox Virus ◽

Tissue Samples

Fowlpox virus (FPV) is one of the viruses affecting chickens worldwide, causing pathological and economic losses in the poultry industry. Viral lesions are easily recognizable by the eye and usually appear in the featherless areas, especially the head. Moreover, the virus could lead to blindness and mortality in some cases. This study diagnosed the suspected fowlpox cases, identified and classified the causative agent. We also analyzed the differences and similarities of closely related viruses at the neighboring and regional countries. Fifty samples were collected from three locations of Tikrit city from the domesticated chickens, which showed cutaneous lesions. Virus DNA was extracted directly from tissue samples before the nested PCR technique was performed. The virion core protein (P4b) gene is partially sequenced and analyzed with routine histological sectioning. Results showed that the virus causes pock lesions of dermal hyperplasia and hyperkeratosis. Hyperplasia and congestion of the chorioallantoic membrane were also recorded. The study also showed that the DNA of FPV could be extracted directly from animal tissue without further purification. The sequence analysis showed that the FPV was confirmed in all samples clustered in clade A identical with Iranian and Egyptian isolates. In conclusion, this study approved that the virus belongs to the classical dermal type of poxviruses and the short genetic distances between viruses related to closely neighboring countries. We also concluded that the conservative P4b gene included mutation sites that make this gene practical for diagnosing the virus and phylogenetic analysis.

Download Full-text

An improved method for semiquantification of gene amplification from archival material.

Genome Research ◽

10.1101/gr.4.3.178 ◽

1994 ◽

Vol 4 (3) ◽

pp. 178-184 ◽

Cited By ~ 1

Author(s):

M A Underwood ◽

J M Bartlett ◽

T G Cooke

Keyword(s):

Gene Amplification ◽

Improved Method ◽

Archival Material

Download Full-text

Determination of Vitamin B12 With an Escherichia Coli Mutant

Clinical Chemistry ◽

10.1093/clinchem/4.1.22 ◽

1958 ◽

Vol 4 (1) ◽

pp. 22-26 ◽

Cited By ~ 8

Author(s):

J Aronovitch ◽

Nathan Grossowicz

Keyword(s):

Escherichia Coli ◽

Vitamin B12 ◽

Animal Tissue ◽

Animal Origin ◽

Improved Method ◽

Sensitive Mutant ◽

E Coli ◽

Tissue Extracts ◽

Human Sera

Abstract An improved method for the determination of vitamin B12, using a more sensitive mutant of Escherichia coli, which enables determination of 4-20 µµg./ml. of the vitamin, has been described. Comparative determinations of vitamin B12 content of human sera and of animal tissue extracts gave similar values when assayed with both E. coli and O. malhamensis. Although O. malhamensis is more specific in its response to cyanocobalamin than E. coli, the latter organism seems just as reliable for determination of vitamin B12 in serum and in tissue of human and animal origin. The simplicity, rapidity, and sensitivity of the improved E. coli assay make it very suitable for clinical use.

Download Full-text

An Improved Method for Measuring Absolute Metabolite Concentrations in Small Biofluid or Tissue Samples

Journal of Proteome Research ◽

10.1021/acs.jproteome.8b00773 ◽

2019 ◽

Vol 18 (4) ◽

pp. 1503-1512 ◽

Cited By ~ 1

Author(s):

Tharindu Fernando ◽

Annick Sawala ◽

Andrew P. Bailey ◽

Alex P. Gould ◽

Paul C. Driscoll

Keyword(s):

Improved Method ◽

Tissue Samples ◽

Metabolite Concentrations

Download Full-text

Characterization of a New Monoclonal Antibody Against PAX5/BASP in 1525 Paraffin-embedded Human and Animal Tissue Samples

Applied Immunohistochemistry & Molecular Morphology ◽

10.1097/pai.0b013e3181e79013 ◽

2010 ◽

Vol 18 (6) ◽

pp. 561-572 ◽

Cited By ~ 15

Author(s):

Claudio Agostinelli ◽

Elena Sabattini ◽

Jakob Oemar Gjørret ◽

Simona Righi ◽

Maura Rossi ◽

...

Keyword(s):

Monoclonal Antibody ◽

Animal Tissue ◽

Tissue Samples

Download Full-text

High Resolution Immuno-Electron Microscopy Reveals That Fetal Skin Contains Microfibrils which are Heteropolymers of Fibrillin-1 and Fibrillin-2

Microscopy and Microanalysis ◽

10.1017/s1431927600025939 ◽

1998 ◽

Vol 4 (S2) ◽

pp. 1162-1163

Author(s):

D.R. Keene ◽

N.L. Charbonneau ◽

B.J. Dzamba ◽

D.P. Reinhardt ◽

C.C. Ridgway ◽

...

Keyword(s):

Electron Microscopy ◽

Monoclonal Antibodies ◽

Electron Microscope ◽

Fetal Development ◽

Human Tissues ◽

Fetal Skin ◽

Transmission Electron ◽

Immuno Electron Microscopy ◽

The Matrix ◽

Fibrillin 1

Immunolocalization studies of neonate and older human tissues have previously demonstrated that fibrillin-1 is a component of elastin and non-elastin associated microfibrils. In sections taken from fixed, dehydrated and embedded tissue, microfibrils appear in the transmission electron microscope as hollow rods, 6-8 nm in diameter. When isolated from tissue by homogenization and observed following negative staining or rotary shadowing, microfibrils appear as beaded strings with a degree of extendibility. Recently, a closely related glycoprotein, fibrillin-2, has been described, which is expressed in early fetal development, prior to fibrillin-1, but then disappears in most tissues just prior to birth. We demonstrate here the characterization monoclonal antibodies specific for fibrillin-2. The antibodies are shown by ELISA and immunoblots to be fibrillin-2 specific. The matrix from a variety of tissues, including aorta, tendon, and eye are shown to contain fibrillin-2 by immunofluorescence in a pattern similar to that of fibrillin-1.

Download Full-text