PO-1610 Proton and carbon range verification for anatomy-like objects with the use of animal tissue samples

2021 ◽  
Vol 161 ◽  
pp. S1331-S1332
Author(s):  
J. Gora ◽  
M. Bolsa-Ferruz ◽  
S. Vatnitsky ◽  
G. Kragl ◽  
A. Carlino ◽  
...  
Keyword(s):  
Animal Tissue ◽  
Tissue Samples ◽  
Range Verification

Immunoelectron microscopic localization of elastic tissue in archival material using an improved method

1990 ◽  
Vol 48 (3) ◽  
pp. 794-795
Author(s):  
J. C. Fanning ◽  
J. F. White ◽  
R. Polewski ◽  
E. G. Cleary
Keyword(s):  
Normal Animal ◽  
Animal Tissue ◽  
Elastic Tissue ◽  
Human Tissues ◽  
Improved Method ◽  
Tissue Samples ◽  
Archival Material ◽  
Immuno Electron Microscopy ◽  
Arteries And Veins ◽  
Biochemical Examination

Elastic tissue is an important component of the walls of arteries and veins, of skin, of the lungs and in lesser amounts, of many other tissues. It is responsible for the rubber-like properties of the arteries and for the normal texture of young skin. It undergoes changes in a number of important diseases such as atherosclerosis and emphysema and on exposure of skin to sunlight.We have recently described methods for the localizationof elastic tissue components in normal animal and human tissues. In the study of developing and diseased tissues it is often not possible to obtain samples which have been optimally prepared for immuno-electron microscopy. Sometimes there is also a need to examine retrospectively samples collected some years previously. We have therefore developed modifications to our published methods to allow examination of human and animal tissue samples obtained at surgery or during post mortem which have subsequently been: 1. stored frozen at -35° or -70°C for biochemical examination; 2.

scholarly journals Pathological, molecular and phylogenic study of fowlpox virus in domesticated chickens of Tikrit City, Iraq

2021 ◽  
Vol 58 ◽  
pp. e176255
Author(s):  
Ismael Ibrahim Hasan ◽  
Saad Tawfik Rasheed ◽  
Mohammed Khorshid Shakor
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Core Protein ◽  
Chorioallantoic Membrane ◽  
Genetic Distances ◽  
Animal Tissue ◽  
Economic Losses ◽  
Cutaneous Lesions ◽  
Fowlpox Virus ◽  
Tissue Samples

Fowlpox virus (FPV) is one of the viruses affecting chickens worldwide, causing pathological and economic losses in the poultry industry. Viral lesions are easily recognizable by the eye and usually appear in the featherless areas, especially the head. Moreover, the virus could lead to blindness and mortality in some cases. This study diagnosed the suspected fowlpox cases, identified and classified the causative agent. We also analyzed the differences and similarities of closely related viruses at the neighboring and regional countries. Fifty samples were collected from three locations of Tikrit city from the domesticated chickens, which showed cutaneous lesions. Virus DNA was extracted directly from tissue samples before the nested PCR technique was performed. The virion core protein (P4b) gene is partially sequenced and analyzed with routine histological sectioning. Results showed that the virus causes pock lesions of dermal hyperplasia and hyperkeratosis. Hyperplasia and congestion of the chorioallantoic membrane were also recorded. The study also showed that the DNA of FPV could be extracted directly from animal tissue without further purification. The sequence analysis showed that the FPV was confirmed in all samples clustered in clade A identical with Iranian and Egyptian isolates. In conclusion, this study approved that the virus belongs to the classical dermal type of poxviruses and the short genetic distances between viruses related to closely neighboring countries. We also concluded that the conservative P4b gene included mutation sites that make this gene practical for diagnosing the virus and phylogenetic analysis.

Characterization of a New Monoclonal Antibody Against PAX5/BASP in 1525 Paraffin-embedded Human and Animal Tissue Samples

2010 ◽  
Vol 18 (6) ◽  
pp. 561-572 ◽  
Author(s):  
Claudio Agostinelli ◽  
Elena Sabattini ◽  
Jakob Oemar Gjørret ◽  
Simona Righi ◽  
Maura Rossi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Animal Tissue ◽  
Tissue Samples

Cleanup with Two Florisil Columns for Gas Chromatographic Determination of Multiple Pyrethroid Insecticides in Products of Animal Origin

1994 ◽  
Vol 77 (6) ◽  
pp. 1634-1638 ◽  
Author(s):  
Guo-Fang Pang ◽  
Tie-Sheng Zhao ◽  
Yan-Zhong Chao ◽  
Chun-Lin Fan
Keyword(s):  
Detection Limit ◽  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Animal Tissue ◽  
Animal Origin ◽  
Tissue Samples ◽  
Pyrethroid Insecticides ◽  
Coefficients Of Variation ◽  
Gas Chromatographic Method

Abstract A gas chromatographic method was developed for the simultaneous determination of 9 pyrethroid insecticides in products of animal origin. The multiresidues of the pyrethroids in different samples were extracted with acetone–petroleum ether (1+1), and extracts were cleaned up on a Florisil partition column and a Florisil adsorption column. Four animal tissue samples were examined at 0.05–0.25 ppm fortification levels. The average recoveries of all insecticides were 76.9–88.0%, and the coefficients of variation were <4.6% for all insecticides except permethrin. The detection limits of the method were ca 5 ppb for all insecticides but permethrin, which had a detection limit of ca 10 ppb.

Method for the Detection of Desmethylbromethalin in Animal Tissue Samples for the Determination of Bromethalin Exposure

2015 ◽  
Vol 63 (21) ◽  
pp. 5146-5151 ◽  
Author(s):  
Michael S. Filigenzi ◽  
Adrienne C. Bautista ◽  
Linda S. Aston ◽  
Robert H. Poppenga
Keyword(s):  
Animal Tissue ◽  
Tissue Samples

scholarly journals Ekstraksi DNA total dari sumber jaringan hewan (Ikan Kerapu) menggunakan metode kit for animal tissue

2019 ◽  
Vol 3 (1) ◽  
pp. 40
Author(s):  
Yanti Ariyanti ◽  
Sister Sianturi
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Dna Analysis ◽  
Early Stage ◽  
Animal Tissue ◽  
High Yield ◽  
Tissue Samples ◽  
Organic Extraction ◽  
Cell Components ◽  
Total Dna

DNA extraction is a series of processes to separate DNA from other cell components. Extraction is the most crucial early stage in molecular research. There are two methods in DNA extraction: traditional organic extraction and non-organic adoption extraction method. Traditional organic extraction method are used by many laboratories to obtain amount of high-yield DNA. However, in recent years it has been a tendency to adopt non-organic commercial protocols, known as extraction kit. This method was relatively faster, low times-consuming and avoid the toxicity due to phenol use. This study aims to determine the steps of DNA extraction to obtain total DNA from muscle tissue of fish grouper using Extraction Kit for Animal Tissue. The Kit extraction method has an advantages that is the processing time is shorter, simple, non-toxic and high-purity DNA yields. A total of 4 tissue samples from grouper muscle were successfully extracted using an Extraction Kit that was indicated by the visualization of the band on qualitative DNA analysis in 1% agarose gel. Keywords: DNA extraction, extraction kit, high-yield DNA, molecular method

scholarly journals Variations in contamination by mercury, cadmium and lead on swine farms in the district of Hodonin in 1994 to 1999

2001 ◽  
Vol 46 (No. 5) ◽  
pp. 132-139 ◽  
Author(s):  
R. Ulrich ◽  
J. Raszyk ◽  
A. Nápravník
Keyword(s):  
Czech Republic ◽  
Environmental Samples ◽  
Animal Tissue ◽  
Tissue Samples ◽  
The Czech Republic ◽  
Swine Farms ◽  
Liver And Kidney ◽  
The Mean ◽  
Cadmium And Lead ◽  
Feed Samples

Environmental samples (n = 254) were collected at regular intervals from 1994 to 1999 on three swine farms in the area of the district town Hodonín, Czech Republic. The samples of feed mixtures (n = 85), barn dust sediments (n = 44), and muscular (n = 42), hepatic (n = 41), and renal (n = 42) tissues of feeder pigs were analysed for the concentrations of mercury, cadmium and lead. The concentrations of mercury showed decreasing tendencies in animal tissue samples (1994–1996). Compared with the mean for the Czech Republic, the concentration of mercury was markedly higher in liver and kidney samples (0.064 mg/kg and 0.114 mg/kg, respectively) collected in 1998, the concentration of cadmium was moderately higher in feed, muscle, liver, and kidney samples collected in 1996–1998. Statutory limits were exceeded in two liver and two kidney samples and one feed sample only. The concentration of lead in muscle, kidney, and feed samples (1994–1996) and barn dust samples (1996–1998) showed also a decreasing tendency. However, the limit was exceeded in 16 muscle samples (0.11 to 0.23 mg/kg) collected in 1994–1999 and two kidney samples collected in 1996 (0.74 mg/kg) and 1999 (0.77 mg/kg). Heavy metals present in dust sediments apparently did not contribute significantly to their content in animal tissue. While the health risk resulting from the contents of mercury and cadmium can be classified as moderate, the contents of lead must be regarded as markedly more dangerous.

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