Membranous diffusion of Dil (a phospholipid-like fluorescent probe) in retrogradely labeled neurons: Analysis with the EM

1990 ◽  
Vol 48 (3) ◽  
pp. 896-897
Author(s):  
D.E. Cunningham ◽  
C.S. von Bartheld ◽  
E.W Rubel
Keyword(s):  
Fluorescent Probe ◽  
Phosphate Buffer ◽  
Lateral Diffusion ◽  
Chick Embryos ◽  
White Leghorn ◽  
Fixed Tissue ◽  
Normal Diffusion ◽  
Intracellular Pathways ◽  
Diffusion Properties

The compound 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (Dil) is a fluorescent molecule with diffusion properties similar to those of phospholipids. Intramembranous (“lateral”) diffusion of Dil is preserved in aldehyde-fixed tissues and has been used to label neuronal pathways. We found that Dil-label, when illuminated (“photo-converted”) in the presence of diaminobenzidine (DAB), forms an electron-dense reaction product that is suitable for EM analysis. We show that the Dil molecule has a surprising diffusion ability and differential affinitiy to internal membranes after retrograde diffusion along neurites in fixed tissue. This finding may have implications for normal diffusion of phospholipids along intracellular pathways in vivo.Two 20-day old White Leghorn chick embryos were anesthetized and perfused intracardially with 0.1M phosphate buffer (PB, pH 7.4) followed by perfusion with 2% paraformaldehyde and 1% glutaraldehyde in PB.

Dexamethasone Effect on Embryonic Chick Respiratory Epithelium

1982 ◽  
Vol 40 ◽  
pp. 248-249
Author(s):  
M.R. Richter ◽  
R.V. Blystone
Keyword(s):  
Lung Development ◽  
Critical Role ◽  
Respiratory Epithelium ◽  
Chick Embryos ◽  
Embryonic Chick ◽  
White Leghorn ◽  
Lung Maturation ◽  
Synthetic Analogs ◽  
Lung Physiology ◽  
Avian Lung

Dexamethasone and other synthetic analogs of corticosteroids have been employed clinically as enhancers of lung development. The mechanism(s) by which this steroid induction of later lung maturation operates is not clear. This study reports the effect on lung epithelia of dexamethasone administered at different intervals during development. White Leghorn chick embryos were used so as to remove possible maternal and placental influences on the exogenously applied steroid. Avian lung architecture does vary from mammals; however, respiratory surfactant produced by the lung epithelia serves an equally critical role in avian lung physiology.

Development of the Foramen Secundum in the Chick as Observed by Scanning Electron Microscopy

1976 ◽  
Vol 34 ◽  
pp. 212-213
Author(s):  
M.J.C. Hendrix ◽  
D.E. Morse
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Procaine Hydrochloride ◽  
Cardiac Anomaly ◽  
Chick Embryos ◽  
Congenital Cardiac Anomaly ◽  
Septal Defects ◽  
Critical Point Drying ◽  
Scanning Electron

Atrial septal defects are considered the most common congenital cardiac anomaly occurring in humans. In studying the normal sequential development of the atrial septum, chick embryos of the White Leghorn strain were prepared for scanning electron microscopy and the results were then extrapolated to the human heart. One-hundred-eighty chick embryos from 2 to 21 days of age were removed from their shells and immersed in cold cacodylate-buffered aldehyde fixative . Twenty-four embryos through the first week post-hatching were perfused in vivo using cold cacodylate-buffered aldehyde fixative with procaine hydrochloride. The hearts were immediately dissected free and remained in the fixative a minimum of 2 hours. In most cases, the lateral atrial walls were removed during this period. The tissues were then dehydrated using a series of ascending grades of ethanol; final dehydration of the tissues was achieved via the critical point drying method followed by sputter-coating with goldpalladium.

In vivo assessing colitis severity by topical administration of fluorescent probe against neutrophils

Talanta ◽  
2021 ◽  
pp. 122519
Author(s):  
Yi Li ◽  
Chang Li ◽  
Yuanbiao Tu ◽  
Ji Tao ◽  
Peifei Liu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Topical Administration

Nanoscale Chlorophyll-Liposome Composite (NCLC) Fluorescent Probe for In Vivo Bio-imaging

2017 ◽  
Vol 28 (5) ◽  
pp. 2969-2977
Author(s):  
K. S. Uma Suganya ◽  
K. Govindaraju ◽  
C. Veena Vani ◽  
R. Kirubagaran ◽  
T. Ashok Kumar ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Bio Imaging

scholarly journals Loss of potassium from embryonic chick hearts in potassium-free media with and without calcium

1938 ◽  
Vol 124 (837) ◽  
pp. 446-450
Keyword(s):  
Phosphate Buffer ◽  
Direct Evidence ◽  
Watch Glass ◽  
Chick Embryos ◽  
Embryonic Chick ◽  
Intercellular Spaces ◽  
Experimental Solution ◽  
Free Media ◽  
And Control ◽  
Day By Day

Experiments already described (Murray 1938) led to the inference that the cells of the chick embryonic heart lose potassium in potassium-free media. The experiments here described provide direct evidence of this. The hearts were dissected out of 2 ½-3 day chick embryos and placed in the solution PC (Table I) until they had started to beat. They were then thoroughly washed, and were allowed to lie for 5 min. (2 min. in Exp. 1) in the last wash. This last wash is called control A. The solutions used for washing were from the same flasks as the experimental solution. After their passage through control A the hearts were transferred to 2 c.c. of the experimental solution in a Jena watch-glass. After various times in this the hearts were discarded and both the experimental solution and control A were collected. If the experiment extended over more than 1 day the experimental solution and control A were used over again day by day until all the hearts in the experiment had passed through them. The use of control A was necessary for two reasons: ( a ) to show that potassium was not still being washed out of the intercellular spaces at the end of washing ( b ) in experiments lasting over several days the washing solution was fresh each day, but the experimental solution was of course not changed. Hence any small amount of potassium being carried over from the last wash would accumulate in the experimental solution because of the daily increment and might seriously affect the result; but by leaving the hearts for several minutes in the last wash (control A) and by not changing it for fresh on successive days, any such increase would be detected in that solution. In addition to control A, a daily sample (control B) was taken from the same flasks as the solutions used for washing. Details of the solutions are given in Table I ; a phosphate buffer was always used.

Specific Two-Photon Fluorescent Probe for Cysteine Detection in Vivo

2021 ◽  
pp. 120521
Author(s):  
Yu-Xu Tu ◽  
Vijay Natarajan ◽  
Han-Xiang Ko ◽  
Yuan-Pin Lo ◽  
Velmathi Sivan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Two Photon

scholarly journals QUANTITATIVE AND HISTOCHEMICAL STUDIES ON THE DESORPTION AND READSORPTION OF ALDOLASE IN CROSS-STRIATED MUSCLE

1969 ◽  
Vol 17 (5) ◽  
pp. 314-320 ◽  
Author(s):  
H. ARNOLD ◽  
J. NOLTE ◽  
D. PETTE
Keyword(s):  
Enzyme Activity ◽  
Actin Filaments ◽  
Phosphate Buffer ◽  
Striated Muscle ◽  
Psoas Muscle ◽  
Histochemical Staining ◽  
Intracellular Location ◽  
Successive Extraction ◽  
Complete Extraction

Complete extraction of aldolase from minced rabbit psoas muscle was achieved by successive extraction steps in 0.1 M phosphate buffer. Aldolase was then readsorbed quantitatively to the depleted myofibrils. Extraction, readsorption and a final redsorption of the enzyme were followed quantitatively by enzyme activity determinations and qualitatively by histochemical staining of aldolase. The intracellular location of the readsorbed enzyme was found to be identical with that of aldolase in native muscle. In both cases, aldolase was localized within the isotropic bands. These results as well as the previously demonstrated binding of the enzyme to F-actin suggest that aldolase is located within the interfilamentary sarcoplasm of the isotropic bands and is probably also bound in vivo to the actin filaments.

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