scholarly journals Screening for Epstein–Barr virus (EBV) infection status in university freshmen: acceptability of a gingival swab method

2019 ◽  
Vol 147 ◽  
Author(s):  
J. M. Grimm-Geris ◽  
S. K. Dunmire ◽  
L. M. Duval ◽  
E. A. Filtz ◽  
H. J. Leuschen ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
College Freshmen ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Efficacy Trial ◽  
Cross Sectional ◽  
Freshman Year ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

AbstractProphylactic vaccines against Epstein–Barr virus (EBV) are under development. EBV-naïve college freshmen are ideal candidates for an efficacy trial, because their incidence of infectious mononucleosis (mono) during freshman year is as high as 20%. To assess perceptions about mono and a mono vaccine, and to learn if EBV immune status could be determined using a gingival swab rather than phlebotomy, we performed a cross-sectional study of 235 healthy students at the beginning of their freshman year. Subjects completed questionnaires and donated oral washes, gingival swabs and venous blood. Overall, 90% of students found the swab easy to use and 80% preferred the swab over venepuncture. Of the 193 students with sufficient samples, 108 (56%) had EBV antibodies in bloodvs.87 (45.1%) in the gingival swab. The sensitivity and specificity of the swab compared with blood for detecting EBV antibodies was 75.9% and 94.1%, respectively, with an accuracy of 89.3%. EBV DNA was detected in the oral wash and swab of 39.2% and 30.4% of blood-antibody-positive individuals, respectively. In conclusion, 44% of our freshmen were EBV-naïve and thus vaccine candidates, the gingival swab was an acceptable alternative to phlebotomy for detecting EBV antibody but needs improved sensitivity, and the perceived value of EBV vaccine was high (72% believed they would benefit).

Epstein-Barr Virus Load in Whole Blood Correlates With HIV Surrogate Markers and Lymphoma: A French National Cross-Sectional Study

2009 ◽  
Vol 50 (4) ◽  
pp. 427-429 ◽  
Author(s):  
Corinne Amiel ◽  
Jérôme LeGoff ◽  
François Xavier Lescure ◽  
Marianne Coste-Burel ◽  
Claire Deback ◽  
...  
Keyword(s):  
Whole Blood ◽  
Epstein Barr Virus ◽  
Cross Sectional Study ◽  
Surrogate Markers ◽  
Sectional Study ◽  
Cross Sectional ◽  
Virus Load ◽  
Barr Virus ◽  
Epstein Barr

Simultaneous occurrence of Epstein-Barr virus (EBV) in periodontal pockets and in oral squamous cell carcinoma: a cross-sectional study

2021 ◽  
Author(s):  
Humberto Jácome-Santos ◽  
Naira da Silva e Silva ◽  
Renata Gonçalves Resende ◽  
Helder Henrique Costa Pinheiro ◽  
Luiz Fernando Almeida Machado ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Epstein Barr Virus ◽  
Cross Sectional Study ◽  
Simultaneous Occurrence ◽  
Sectional Study ◽  
Cross Sectional ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Association Between Environmental Factors and Oral Epstein-Barr Virus DNA Loads: A Multicenter Cross-sectional Study in China

2018 ◽  
Vol 219 (3) ◽  
pp. 400-409 ◽  
Author(s):  
Yong-Qiao He ◽  
Xiao-Yu Liao ◽  
Wen-Qiong Xue ◽  
Ya-Fei Xu ◽  
Feng-Hua Xu ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Odds Ratio ◽  
Epstein Barr Virus ◽  
Southern China ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Barr Virus ◽  
Associated Diseases ◽  
Epstein Barr

Abstract Background Oral Epstein-Barr virus (EBV) status reflects host EBV activity and potentially links to EBV-associated diseases, however, factors influencing oral EBV loads or reactivation, such as environmental exposures or host factors, are not fully understood. Methods A 2-stage, multicenter, cross-sectional study of 6558 subjects from 21 administrative cities of southern China and 3 populations from representative geographical areas in China (referred to as the south, north, and northeastern populations) was performed. The relationships between demographical factors and environmental exposures to EBV loads were analyzed by logistic regression models. Results Current smoking, with a dose-response effect, was found to be strongly associated with higher oral EBV loads in the pooled data, with an odds ratio of 1.58 (95% confidence interval, 1.39–1.79), as well as in each of the separate populations. The odds ratio increased to 3.06 when current smokers in southern China were compared to never smokers in northern China. Additionally, higher oral EBV loads tended to be detected in older participants, male participants, and participants in southern China. Conclusions This study provided evidence linking the effect of host-environmental factors, particularly smoking, to oral EBV activity. It could strengthen our understanding of the possible causal roles of EBV-related diseases, which may help to prevent or mitigate EBV-associated diseases.

scholarly journals Hubungan Antara Kadar DNA Plasma Virus Epstein Barr Dengan Stadium Klinis Karsinoma Nasofaring WHO Tipe 3

2019 ◽  
Vol 48 (2) ◽  
pp. 165
Author(s):  
Putranti Dyahayu Roziaty ◽  
Soehartono Soehartono ◽  
Hendradi Surjotomo
Keyword(s):  
Computed Tomography ◽  
Ct Scan ◽  
Epstein Barr Virus ◽  
Tumor Type ◽  
Computed Tomography Scan ◽  
Cross Sectional ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr ◽  
Tomography Scan

Latar Belakang: Karsinoma nasofaring (KNF) merupakan keganasan yang tersering ditemukan, dan berdampak pada penurunan kualitas hidup serta memiliki mortalitas tinggi. Penanganan KNF selama ini terkendala oleh waktu tunggu yang cukup lama dalam menentukan staging KNF terutama untuk antrian pemeriksaan computed tomography scan (CT scan) dan Ultrasonography (USG). Pemeriksaan kadar DNA EBV (Deoxyribonucleic acid Epstein-Barr Virus) pada pasien yang relatif lebih mudah dan terjangkau dapat digunakan untuk memprediksi stadium dan prognosis KNF. Dengan mengetahui prognosis KNF lebih dini, maka diharapkan penanganan terhadap KNF dapat segera dilakukan. Tujuan: Mengetahui apakah kadar DNA EBV dapat dipakai untuk memprediksi stadium dan prognosis KNF dengan cara mencari hubungan antara kadar DNA EBV dengan stadium KNF. Metode: Penelitian cross sectional melibatkan 15 subjek penelitian yang terdiagnosis KNF WHO tipe 3 kemudian dilakukan staging dengan CT scan, USG abdomen, dan foto toraks, serta diambil sampel darah untuk diukur kadar DNA EBV. Hasil: Seluruh subjek penelitian mengalami peningkatan kadar DNA EBV sesuai dengan peningkatan stadium KNF. Peningkatan stadium KNF berhubungan signifikan dengan peningkatan kadar DNA EBV (p=0,001). Ukuran tumor (T) berhubungan signifikan dengan kadar DNA EBV (p=0,023), ukuran nodul (N) berhubungan signifikan dengan kadar DNA EBV (p=0,005), ada tidaknya metastasis tidak berhubungan signifikan dengan kadar DNA EBV (p=0,398). Nilai cut off kadar DNA EBV sebesar 952 kopi/ml. Kesimpulan: Terdapat hubungan yang signifikan antara kadar DNA EBV dengan stadium klinis, dengan demikian kadar DNA EBV dapat dipertimbangkan untuk digunakan sebagai prediktor stadium dan prognosis KNF. Background: Nasopharyngeal carcinoma (NPC) is the predominant tumor type arising in the nasopharynx,  with a high mortality and affecting quality of life.  NPC treatment  management is hindered by long queues of Computed Tomography Scan (CT scan) and Ultrasonography (USG) examinations to ascertain the NPC staging. The examination of Epstein-Barr Virus (EBV) DNA level is relatively simpler and inexpensive  to predict the NPC staging and prognosis, thus, it can speed up NPC treatment. Objective: To determine whether EBV DNA level can be used to predict the NPC  stage and prognosis by finding a correlation between EBV DNA level and NPC stage. Method: This was  a cross-sectional study involving 15 respondents who were diagnosed as WHO type 3 NPC, and examined by CT scan, abdominal ultrasound, chest X-ray,  and blood  test for measuring the levels of EBV DNA to determine the stage. Results: All respondents had elevated levels of EBV DNA in accordance with NPC stage elevation. Increased NPC stages were significantly correlated with elevated levels of EBV DNA (p=0.001). The size of tumor (T) was significantly correlated with EBV DNA (p=0.023), the size of nodule (N) was significantly correlated with EBV DNA (p=0.005).  The presence or absence of metastasis did not significantly correlate with EBV DNA (p=0.398). The EBV DNA cut off value was 952 copies/ml. Conclusions: There  was a significant correlation between EBV DNA levels and clinical stages, hence EBV DNA can be considered to be used as NPC staging and prognosis predictor.   

scholarly journals COMPARISON OF POLYMORPHISM rs3813865 CYTOCHROME P450 FAMILY 2 SUBFAMILY E POLYPEPTIDE 1 (CYP2E1) GENE IN VARIOUS CLINICAL STAGE OF UNDIFFERENTIATED TYPE NASOPHARYNGEAL CARCINOMA

2019 ◽  
Vol 12 (04) ◽  
pp. 2055-2061
Author(s):  
I Ketut Suanda ◽  
I Gde Ardika Nuaba ◽  
Ni Made Alit Ardianti
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Clinical Stage ◽  
Univariate Analysis ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Barr Virus ◽  
Cyp2e1 Gene ◽  
Undifferentiated Type ◽  
Epstein Barr

Nasopharyngeal carcinoma is caused by interaction of Epstein-Barr virus chronic infection, environtment, and genetic factors. Epstein-Barr virus (EBV) infect nasopharyngeal ephitelial cell in latent period. This infection will cause mutation and further causing malignancy. This is a cross-sectional study in undifferentiated type NPC patients after hystopatological examination and were examined in RSUP Sanglah Denpasar from January 2017 to December 2018. This study is using 62 subjects who meets inclusion criteria. Univariate analysis was done to show subject characteristics which include age, gender, occupation, clinical stage, gene allele and rs3813865 polymorphism CYP2E1 gene. Mean age of subject is 48.05 years with standard deviation of 10.86 years. The youngest is 17 years old and the oldest is 73 years old. The most are men as many as 47 subjects (75.8%), and the most occupation are government employee as many as 17 subjects (27.4%). The most clinical stage of undifferentiated type NPC are stage II as many as 7 subjects (11.3%). Based on TNM, the most are T4 as many as 32 subjects (51.6%), N3 as many as 21 subjects (33.9%), and M0 as many as 60 subjects (96.8%).

scholarly journals The frequency of Epstein-Barr virus among hemodialysis patients, Ahvaz, Iran

2019 ◽  
Author(s):  
Rahil Nahid Nahid Samiei ◽  
Shahab Mahmoudvand ◽  
Somayeh Shokri ◽  
Manoochehr Makvandi ◽  
Heshmatol- lah Shahbazian ◽  
...  
Keyword(s):  
Organ Transplantation ◽  
Epstein Barr Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Cross Sectional ◽  
Barr Virus ◽  
Ebv Reactivation ◽  
Study Results ◽  
Ebv Dna ◽  
Epstein Barr

Background and Objectives: Epstein-Barr virus (EBV) has infected more than 90% of adults worldwide. EBV infection is asymptomatic in healthy individuals and is controlled by a robust immune response while in individuals with weakened immunesystems including Hemodialysis (HD) patients and transplant recipients leads to serious illnesses. This study was aimed to investigate the frequency of EBV among the HD patients. Materials and Methods: The cross-sectional study was carried out on 84 HD patients. These sera were checked for an- ti-EBV (VCA) IgG Ab assessment using enzyme-linked immunosorbent assay (ELISA). The DNA was extracted from the sera samples and tested for EBV DNA using nested PCR. Results: 52/84 (61.9%) of HD were males and 32/84 (38.1%) were females. The average age of participants was varying from 18 to 85 years while the mean age was 52 ± 1.57 SD years. 81 of 84 (96.42%); including 49/52 (94.23%) male and 32/32 (100%) female, were positive for anti-EBV (VCA) IgG antibody while 3 (3.58%) were negative. No significant dif- ferences were observed between the subjects regarding gender (P=0.28). EBV DNA was detected in 7 (8.33%) individuals, including 6 (11.53%) and 1 (3.12%) in male and female, respectively (P=0.24). Conclusion: Our study results showed that high prevalence of anti-EBV (VCA) IgG antibody (96.42%) were observed among the HD patients. Although the status of EBV latency was not performed, but it seems many of these patients are at risk of EBV-reactivation during the organ transplantation. As a result, it is recommended that the detection of EBNA-1 gene as a marker of EBV latency should be implemented for all HD patients to prevent EBV reactivation during organ transplantation.

scholarly journals Deteksi Gen Litik BRLF1 Epstein-Barr Virus pada Penderita Karsinoma Nasofaring

Biosfera ◽  
2018 ◽  
Vol 35 (1) ◽  
pp. 29
Author(s):  
Tri Yulia Ningsih ◽  
Daniel Joko Wahyono ◽  
Nur Signa Aini Gumilas
Keyword(s):  
Molecular Markers ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Venous Blood ◽  
Lytic Cycle ◽  
Conventional Pcr ◽  
Cross Sectional ◽  
Barr Virus ◽  
Pcr Method ◽  
Epstein Barr

Rosenmuller fossa. Epithelial malignancy is often found in Chinese populations and Southeast Asia including Indonesia. Undifferentiated nasopharyngeal carcinoma (NPC WHO-3) type is 100% associated with Epstein-Barr virus (EBV) infection. Bam-HI R Leftward Reading Frame 1 (BRLF1) lytic gene has an important function as a transition mediator of latent phase to the lytic phase in EBV cycle. Detection of BRLF1 gene by PCR can be used for NPC diagnosis. The aim of this study is to identify BRLF1 lytic genes as molecular markers of Epstein-Barr virus in nasopharyngeal carcinoma patients with conventional PCR method and to determine the sensitivity of conventional PCR method to detect BRLF1 gene. The research design was cross sectional study. A total of 22 DNA samples were isolated from venous blood of NPC patients from RSUD Prof dr Margono Soekarjo, Purwokerto with informed consent. BRLF1 gene identification is done with conventional PCR technique. The results of this research showed that BRLF1 genes as molecular markers lytic cycle of Epstein-Barr virus in nasopharyngeal carcinoma patients can be identified conventional PCR technique that will produced DNA 157 bp. BRLF1 gene was detected in 16 samples (72.73%) of 22 samples of this study.

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